P69484 (VP2_CAVC1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dual specificity protein phosphatase VP2
EC=3.1.3.16
EC=3.1.3.48

Gene names

Name:

VP2

Organism

Chicken anemia virus (isolate Germany Cuxhaven-1) (CAV) [Reference proteome]

Taxonomic identifier

73475 [NCBI]

Taxonomic lineage

Viruses › ssDNA viruses › Circoviridae › Gyrovirus › Chicken anemia virus

Virus host

Gallus gallus (Chicken) [TaxID: 9031]

Protein attributes

Sequence length	216 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May act as a scaffold protein in virion assembly. May also play a role in intracellular signaling during viral replication. Ref.4
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. A phosphoprotein + H2O = a protein + phosphate.
Induction	VP1 and VP2 are detected 12 hours post infection, while VP3 only after 24 hours. Ref.3
Sequence similarities	Belongs to the gyrovirus protein VP2 family.

Ontologies

Keywords
Developmental stage	Early protein
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	peptidyl-tyrosine dephosphorylation Inferred from electronic annotation. Source: GOC
Molecular_function	protein tyrosine phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 216	216	Dual specificity protein phosphatase VP2		PRO_0000223004
Sites
	Active site	95	1	Phosphocysteine intermediate By similarity
Experimental info
	Sequence conflict	153	1	V → A in AAA91822. Ref.1
	Sequence conflict	187	1	D → N in AAA42882. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P69484 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: F82B69EF88BDDE52	FASTA	216	24,139
	10 20 30 40 50 60 MHGNGGQPAA GGSESALSRE GQPGPSGAAQ GQVISNERSP RRYSTRTING VQATNKFTAV 70 80 90 100 110 120 GNPSLQRDPD WYRWNYNHSI AVWLRECSRS HAKICNCGQF RKHWFQECAG LEDRSTQASL 130 140 150 160 170 180 EEAILRPLRV QGKRAKRKLD YHYSQPTPNR KKVYKTVRWQ DELADREADF TPSEEDGGTT 190 200 210 SSDFDEDINF DIGGDSGIVD ELLGRPFTTP APVRIV

References

[1]	"Characterization of cloned chicken anemia virus DNA that contains all elements for the infectious replication cycle." Noteborn M.H.M., de Boer G.F., van Roozelaar D.J., Karreman C., Kranenburg O., Vos J.G., Jeurissen S.H.M., Hoeben R.C., Zantema A., Koch G., van Ormondt H., van der Eb A.J. J. Virol. 65:3131-3139(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Characterization of viral DNAs from cells infected with chicken anaemia agent: sequence analysis of the cloned replicative form and transfection capabilities of cloned genome fragments." Meehan B.M., Todd D., Creelan J.L., Earle J.A.P., Hoey E.M., McNulty M.S. Arch. Virol. 124:301-319(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Identification of a 24 kDa protein expressed by chicken anaemia virus." Douglas A.J., Phenix K., Mawhinney K.A., Todd D., Mackie D.P., Curran W.L. J. Gen. Virol. 76:1557-1562(1995) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
[4]	"Simultaneous expression of recombinant baculovirus-encoded chicken anaemia virus (CAV) proteins VP1 and VP2 is required for formation of the CAV-specific neutralizing epitope." Noteborn M.H., Verschueren C.A., Koch G., Van der Eb A.J. J. Gen. Virol. 79:3073-3077(1998) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M55918 Genomic DNA. Translation: AAA91822.1. M81223 Genomic DNA. Translation: AAA42882.1.
PIR	A39926. A48343.
RefSeq	NP_056773.1. NC_001427.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1494446.
Family and domain databases
InterPro	IPR004118. HEV_TT_virus_Orf2/Gyrovir_Vp2. [Graphical view]
Pfam	PF02957. TT_ORF2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

VP2_CAVC1

Accession

Primary (citable) accession number: P69484
Secondary accession number(s): P54091, Q99151

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	April 3, 2013
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program