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Protein

Carbohydrate sulfotransferase 11

Gene

Chst11

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the transfer of sulfate to position 4 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Can also sulfate Gal residues in desulfated dermatan sulfate. Preferentially sulfates in GlcA->GalNAc unit than in IdoA->GalNAc unit. Does not form 4, 6-di-O-sulfated GalNAc when chondroitin sulfate C is used as an acceptor (By similarity).By similarity

Catalytic activityi

3'-phosphoadenylyl sulfate + chondroitin = adenosine 3',5'-bisphosphate + chondroitin 4'-sulfate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi124 – 130PAPSBy similarity7
Nucleotide bindingi186 – 194PAPSBy similarity9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Carbohydrate metabolism

Enzyme and pathway databases

ReactomeiR-RNO-2022870. Chondroitin sulfate biosynthesis.
SABIO-RKP69478.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbohydrate sulfotransferase 11 (EC:2.8.2.5)
Alternative name(s):
Chondroitin 4-O-sulfotransferase 1
Chondroitin 4-sulfotransferase 1
Short name:
C4S-1
Short name:
C4ST-1
Short name:
C4ST1
Gene namesi
Name:Chst11
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi1308400. Chst11.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 16CytoplasmicSequence analysisAdd BLAST16
Transmembranei17 – 37Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini38 – 352LumenalSequence analysisAdd BLAST315

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001896661 – 352Carbohydrate sulfotransferase 11Add BLAST352

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi205N-linked (GlcNAc...)Sequence analysis1
Glycosylationi223N-linked (GlcNAc...)Sequence analysis1
Glycosylationi321N-linked (GlcNAc...)Sequence analysis1
Glycosylationi342N-linked (GlcNAc...)By similarity1

Post-translational modificationi

N-glycosylated; required for activity and stability.By similarity

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiP69478.
PRIDEiP69478.

Expressioni

Gene expression databases

BgeeiENSRNOG00000008885.
ExpressionAtlasiP69478. baseline and differential.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012017.

Family & Domainsi

Sequence similaritiesi

Belongs to the sulfotransferase 2 family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4651. Eukaryota.
ENOG4111GJR. LUCA.
GeneTreeiENSGT00760000119214.
HOGENOMiHOG000231801.
HOVERGENiHBG050952.
InParanoidiP69478.
KOiK01017.
OMAiPLNEHWQ.
OrthoDBiEOG091G0FZO.
PhylomeDBiP69478.
TreeFamiTF325581.

Family and domain databases

InterProiIPR018011. Carb_sulfotransferase-rel.
IPR005331. Sulfotransferase.
[Graphical view]
PANTHERiPTHR12137. PTHR12137. 1 hit.
PfamiPF03567. Sulfotransfer_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P69478-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKPALLEVMR MNRICRMVLA TCLGSFILVI FYFQSMLHPV MRRNPFGVDI
60 70 80 90 100
CCRKGSRSPL QELYNPIQLE LSNTAILHQM RRDQVTDTCR ANSAMSRKRR
110 120 130 140 150
VLTPNDLKHL VVDEDHELIY CYVPKVACTN WKRLMMVLSG RGKYSDPMEI
160 170 180 190 200
PANEAHVSAN LKTLNQYSIP EINHRLKSYM KFLFVREPFE RLVSAYRNKF
210 220 230 240 250
TQKYNTSFHK RYGTKIIRRQ RKNATQEALR KGDDVKFEEF VAYLIDPHTQ
260 270 280 290 300
REEPFNEHWQ TVYSLCHPCH IHYDLVGKYE TLEEDSNYVL RLAGVSGYLK
310 320 330 340 350
FPTYAKSTRT TDEMTTEFFQ NISAEHQTQL YEVYKLDFLM FNYSVPNYLK

LD
Length:352
Mass (Da):41,626
Last modified:March 15, 2005 - v1
Checksum:i3A693D10430A2EA4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti277G → Q AA sequence (PubMed:10722746).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03057909 Genomic DNA. No translation available.
AABR03059893 Genomic DNA. No translation available.
AABR03062052 Genomic DNA. No translation available.
AABR03060128 Genomic DNA. No translation available.
AABR03059314 Genomic DNA. No translation available.
AABR03058261 Genomic DNA. No translation available.
AABR03058513 Genomic DNA. No translation available.
AABR03056595 Genomic DNA. No translation available.
AABR03057359 Genomic DNA. No translation available.
AABR03058222 Genomic DNA. No translation available.
AABR03059499 Genomic DNA. No translation available.
AABR03058053 Genomic DNA. No translation available.
AABR03058580 Genomic DNA. No translation available.
AABR03056571 Genomic DNA. No translation available.
AABR03061700 Genomic DNA. No translation available.
AABR03056958 Genomic DNA. No translation available.
RefSeqiNP_001101549.1. NM_001108079.1.
XP_017450314.1. XM_017594825.1.
UniGeneiRn.105288.

Genome annotation databases

EnsembliENSRNOT00000012017; ENSRNOP00000012017; ENSRNOG00000008885.
GeneIDi314694.
KEGGirno:314694.
UCSCiRGD:1308400. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03057909 Genomic DNA. No translation available.
AABR03059893 Genomic DNA. No translation available.
AABR03062052 Genomic DNA. No translation available.
AABR03060128 Genomic DNA. No translation available.
AABR03059314 Genomic DNA. No translation available.
AABR03058261 Genomic DNA. No translation available.
AABR03058513 Genomic DNA. No translation available.
AABR03056595 Genomic DNA. No translation available.
AABR03057359 Genomic DNA. No translation available.
AABR03058222 Genomic DNA. No translation available.
AABR03059499 Genomic DNA. No translation available.
AABR03058053 Genomic DNA. No translation available.
AABR03058580 Genomic DNA. No translation available.
AABR03056571 Genomic DNA. No translation available.
AABR03061700 Genomic DNA. No translation available.
AABR03056958 Genomic DNA. No translation available.
RefSeqiNP_001101549.1. NM_001108079.1.
XP_017450314.1. XM_017594825.1.
UniGeneiRn.105288.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000012017.

Proteomic databases

PaxDbiP69478.
PRIDEiP69478.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000012017; ENSRNOP00000012017; ENSRNOG00000008885.
GeneIDi314694.
KEGGirno:314694.
UCSCiRGD:1308400. rat.

Organism-specific databases

CTDi50515.
RGDi1308400. Chst11.

Phylogenomic databases

eggNOGiKOG4651. Eukaryota.
ENOG4111GJR. LUCA.
GeneTreeiENSGT00760000119214.
HOGENOMiHOG000231801.
HOVERGENiHBG050952.
InParanoidiP69478.
KOiK01017.
OMAiPLNEHWQ.
OrthoDBiEOG091G0FZO.
PhylomeDBiP69478.
TreeFamiTF325581.

Enzyme and pathway databases

ReactomeiR-RNO-2022870. Chondroitin sulfate biosynthesis.
SABIO-RKP69478.

Miscellaneous databases

PROiP69478.

Gene expression databases

BgeeiENSRNOG00000008885.
ExpressionAtlasiP69478. baseline and differential.

Family and domain databases

InterProiIPR018011. Carb_sulfotransferase-rel.
IPR005331. Sulfotransferase.
[Graphical view]
PANTHERiPTHR12137. PTHR12137. 1 hit.
PfamiPF03567. Sulfotransfer_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCHSTB_RAT
AccessioniPrimary (citable) accession number: P69478
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: March 15, 2005
Last modified: November 30, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.