Reviewed, UniProtKB/Swiss-Prot P69476 (NEP1_NEPDI)

Names and origin

Protein names	Recommended name:     Aspartic proteinase nepenthesin-1     EC=3.4.23.12 Alternative name(s):     Nepenthesin-I
Organism	Nepenthes distillatoria (Pitcher plant)
Taxonomic identifier	122309 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Nepenthaceae › Nepenthes

Protein attributes

Sequence length	164 AA.
Sequence status	Fragments.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Extracellular proteinase found in the pitcher fluid of carnivorous plants. Digest prey for nitrogen uptake.
Catalytic activity	Similar to pepsin, but also cleaves on either side of Asp and at Lys-|-Arg.
Enzyme regulation	Inhibited by pepstatin and by diazoacetyl-D,L-norleucine methyl ester (DAN) in the presence of Cu2+ ions.
Subcellular location	Secreted. Ref.1
Tissue specificity	Parenchymal cells surrounding the secretory glands. Ref.1
Sequence similarities	Belongs to the peptidase A1 family.
Biophysicochemical properties	pH dependence: Optimum pH is 2.6. Retains 95% and 79% of the original activity after incubation for 30 days at pH 3.0 and pH 10.0 respectively. Temperature dependence: Optimum temperature is 55 degrees Celsius. Thermostable up to 50 degrees Celsius. Retains 60% of the original activity after incubation for 30 days at 50 degrees Celsius.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Glycoprotein Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›164	›164	Aspartic proteinase nepenthesin-1		PRO_0000199513
Sites
	Active site	35	1	By similarity
Amino acid modifications
	Glycosylation	93	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	31	1	S → Y
Experimental info
	Non-adjacent residues	55 – 56	2
	Non-adjacent residues	72 – 73	2
	Non-adjacent residues	100 – 101	2
	Non-adjacent residues	113 – 114	2
	Non-adjacent residues	118 – 119	2
	Non-adjacent residues	126 – 127	2
	Non-adjacent residues	148 – 149	2
	Non-terminal residue	164	1

Sequences

	Sequence		Length	Mass (Da)
	P69476-1 [UniParc]. Last modified March 15, 2005. Version 1. Checksum: 60C08491850E4F08	FASTA	164	17,271
	10 20 30 40 50 60 IGPSGVETTV YAGDGEYLMX LSIGTPAQPF SAIMDTGSDL IWTQXQPXTQ XFXQSDPQGS 70 80 90 100 110 120 SSFSTLPCGY GDSETQGSMG TETFTFGSVS IPNITFGXGE GPLPLPXQLD VAKYITLDLP 130 140 150 160 IDPSAFDLCF QTPSDPSNLQ IPTFVMHFDT GNSVVSFVSA QCGA

References

[1]

"Enzymic and structural characterization of nepenthesin, a unique member of a novel subfamily of aspartic proteinases." Athauda S.B.P., Matsumoto K., Rajapakshe S., Kuribayashi M., Kojima M., Kubomura-Yoshida N., Iwamatsu A., Shibata C., Inoue H., Takahashi K. Biochem. J. 381:295-306(2004) [PubMed: 15035659] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, VARIANT TYR-31. Tissue: Pitcher.

Cross-references

3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.4.23.12. 275086.
Family and domain databases
InterPro	IPR001461. Peptidase_A1. IPR001969. Peptidase_aspartic_AS. [Graphical view]
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF00026. Asp. 1 hit. [Graphical view]
PROSITE	PS00141. ASP_PROTEASE. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NEP1_NEPDI

Accession

Primary (citable) accession number: P69476

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 15, 2005
Last modified:	June 16, 2009
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)