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P69452

- LCFA_ECOL6

UniProt

P69452 - LCFA_ECOL6

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Protein
Long-chain-fatty-acid--CoA ligase
Gene
fadD, c2209
Organism
Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids By similarity.

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi213 – 22412ATP Inferred
Add
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. long-chain fatty acid-CoA ligase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Long-chain-fatty-acid--CoA ligase (EC:6.2.1.3)
Alternative name(s):
Long-chain acyl-CoA synthetase
Short name:
Acyl-CoA synthetase
Gene namesi
Name:fadD
Ordered Locus Names:c2209
OrganismiEscherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
Taxonomic identifieri199310 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000001410: Chromosome

Subcellular locationi

Membrane; Peripheral membrane protein By similarity
Note: Partially membrane-associated By similarity.

GO - Cellular componenti

  1. membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 561561Long-chain-fatty-acid--CoA ligase
PRO_0000193126Add
BLAST

Proteomic databases

PRIDEiP69452.

Interactioni

Subunit structurei

Homodimer Inferred.

Protein-protein interaction databases

STRINGi199310.c2209.

Structurei

3D structure databases

ProteinModelPortaliP69452.
SMRiP69452. Positions 60-551.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000229983.
KOiK01897.
OMAiVATRWHE.
OrthoDBiEOG6MH5BV.

Family and domain databases

InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P69452-1 [UniParc]FASTAAdd to Basket

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MKKVWLNRYP ADVPTEINPD RYQSLVDMFE QSVARYADQP AFVNMGEVMT    50
FRKLEERSRA FAAYLQQGLG LKKGDRVALM MPNLLQYPVA LFGILRAGMI 100
VVNVNPLYTP RELEHQLNDS GASAIVIVSN FAHTLEKVVD KTAVQHVILT 150
RMGDQLSTAK GTVVNFVVKY IKRLVPKYHL PDAISFRSAL HNGYRMQYVK 200
PELVPEDLAF LQYTGGTTGV AKGAMLTHRN MLANLEQVNA TYGPLLHPGK 250
ELVVTALPLY HIFALTINCL LFIELGGQNL LITNPRDIPG LVKELAKYPF 300
TAITGVNTLF NALLNNKEFQ QLDFSSLHLS AGGGMPVQQV VAERWVKLTG 350
QYLLEGYGLT ECAPLVSVNP YDIDYHSGSI GLPVPSTEAK LVDDDDNEVP 400
PGQPGELCVK GPQVMLGYWQ RPDATDEIIK NGWLHTGDIA VMDEEGFLRI 450
VDRKKDMILV SGFNVYPNEI EDVVMQHPGV QEVAAVGVPS GSSGEAVKIF 500
VVKKDPSLTE ESLVTFCRRQ LTGYKVPKLV EFRDELPKSN VGKILRRELR 550
DEARGKVDNK A 561
Length:561
Mass (Da):62,332
Last modified:March 1, 2005 - v1
Checksum:i249B0AA54B3DBFA5
GO

Sequence cautioni

The sequence AAN80668.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014075 Genomic DNA. Translation: AAN80668.1. Different initiation.
PIRiE64941. S41589.
RefSeqiNP_754103.1. NC_004431.1.

Genome annotation databases

EnsemblBacteriaiAAN80668; AAN80668; c2209.
GeneIDi1036653.
KEGGiecc:c2209.
PATRICi18282309. VBIEscCol75197_2071.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE014075 Genomic DNA. Translation: AAN80668.1 . Different initiation.
PIRi E64941. S41589.
RefSeqi NP_754103.1. NC_004431.1.

3D structure databases

ProteinModelPortali P69452.
SMRi P69452. Positions 60-551.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 199310.c2209.

Proteomic databases

PRIDEi P69452.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN80668 ; AAN80668 ; c2209 .
GeneIDi 1036653.
KEGGi ecc:c2209.
PATRICi 18282309. VBIEscCol75197_2071.

Phylogenomic databases

HOGENOMi HOG000229983.
KOi K01897.
OMAi VATRWHE.
OrthoDBi EOG6MH5BV.

Family and domain databases

InterProi IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view ]
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CFT073 / ATCC 700928 / UPEC.

Entry informationi

Entry nameiLCFA_ECOL6
AccessioniPrimary (citable) accession number: P69452
Secondary accession number(s): P29212
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: May 14, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Activity is the highest with fatty acid substrates of > 10 carbon atoms By similarity.

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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