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P69451

- LCFA_ECOLI

UniProt

P69451 - LCFA_ECOLI

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Protein

Long-chain-fatty-acid--CoA ligase

Gene

fadD

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids.

Catalytic activityi

ATP + a long-chain fatty acid + CoA = AMP + diphosphate + an acyl-CoA.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi213 – 22412ATPCuratedAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. fatty acid binding Source: EcoliWiki
  3. long-chain fatty acid-CoA ligase activity Source: EcoCyc
  4. oleic acid binding Source: EcoliWiki

GO - Biological processi

  1. acyl-CoA metabolic process Source: EcoCyc
  2. fatty acid beta-oxidation Source: EcoCyc
  3. fatty acid metabolic process Source: EcoliWiki
  4. lipid metabolic process Source: EcoliWiki
  5. long-chain fatty acid metabolic process Source: GOC
  6. phospholipid biosynthetic process Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:ACYLCOASYN-MONOMER.
ECOL316407:JW1794-MONOMER.
MetaCyc:ACYLCOASYN-MONOMER.
RETL1328306-WGS:GSTH-490-MONOMER.
SABIO-RKP69451.

Protein family/group databases

TCDBi4.C.1.1.4. the proposed fatty acid transporter (fat) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Long-chain-fatty-acid--CoA ligase (EC:6.2.1.3)
Alternative name(s):
Long-chain acyl-CoA synthetase
Short name:
Acyl-CoA synthetase
Gene namesi
Name:fadD
Synonyms:oldD
Ordered Locus Names:b1805, JW1794
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11530. fadD.

Subcellular locationi

Membrane By similarity; Peripheral membrane protein By similarity
Note: Partially membrane-associated.By similarity

GO - Cellular componenti

  1. cytoplasm Source: EcoliWiki
  2. cytoplasmic side of plasma membrane Source: EcoCyc
  3. cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi213 – 2131Y → A: Loss of activity. 1 Publication
Mutagenesisi214 – 2141T → A: 10% of wild-type activity. 1 Publication
Mutagenesisi216 – 2161G → A: Decreases activity. 1 Publication
Mutagenesisi217 – 2171T → A: Decreases activity. 1 Publication
Mutagenesisi219 – 2191G → A: Decreases activity. 1 Publication
Mutagenesisi222 – 2221K → A: Decreases activity. 1 Publication
Mutagenesisi361 – 3611E → A: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 561561Long-chain-fatty-acid--CoA ligasePRO_0000193125Add
BLAST

Proteomic databases

PaxDbiP69451.
PRIDEiP69451.

Expressioni

Gene expression databases

GenevestigatoriP69451.

Interactioni

Subunit structurei

Homodimer.Curated

Protein-protein interaction databases

IntActiP69451. 6 interactions.
STRINGi511145.b1805.

Structurei

3D structure databases

ProteinModelPortaliP69451.
SMRiP69451. Positions 20-551.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0318.
HOGENOMiHOG000229983.
InParanoidiP69451.
KOiK01897.
OMAiVATRWHE.
OrthoDBiEOG6MH5BV.
PhylomeDBiP69451.

Family and domain databases

InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P69451-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKVWLNRYP ADVPTEINPD RYQSLVDMFE QSVARYADQP AFVNMGEVMT
60 70 80 90 100
FRKLEERSRA FAAYLQQGLG LKKGDRVALM MPNLLQYPVA LFGILRAGMI
110 120 130 140 150
VVNVNPLYTP RELEHQLNDS GASAIVIVSN FAHTLEKVVD KTAVQHVILT
160 170 180 190 200
RMGDQLSTAK GTVVNFVVKY IKRLVPKYHL PDAISFRSAL HNGYRMQYVK
210 220 230 240 250
PELVPEDLAF LQYTGGTTGV AKGAMLTHRN MLANLEQVNA TYGPLLHPGK
260 270 280 290 300
ELVVTALPLY HIFALTINCL LFIELGGQNL LITNPRDIPG LVKELAKYPF
310 320 330 340 350
TAITGVNTLF NALLNNKEFQ QLDFSSLHLS AGGGMPVQQV VAERWVKLTG
360 370 380 390 400
QYLLEGYGLT ECAPLVSVNP YDIDYHSGSI GLPVPSTEAK LVDDDDNEVP
410 420 430 440 450
PGQPGELCVK GPQVMLGYWQ RPDATDEIIK NGWLHTGDIA VMDEEGFLRI
460 470 480 490 500
VDRKKDMILV SGFNVYPNEI EDVVMQHPGV QEVAAVGVPS GSSGEAVKIF
510 520 530 540 550
VVKKDPSLTE ESLVTFCRRQ LTGYKVPKLV EFRDELPKSN VGKILRRELR
560
DEARGKVDNK A
Length:561
Mass (Da):62,332
Last modified:March 1, 2005 - v1
Checksum:i249B0AA54B3DBFA5
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti34 – 5118ARYAD…EVMTF → GALRRSTCVCEYGGGNDL in AAA23752. (PubMed:1460045)CuratedAdd
BLAST
Sequence conflicti468 – 49023NEIED…VGVPS → TRLKMSSCSMVAYRKSRLLA YLP in AAA23752. (PubMed:1460045)CuratedAdd
BLAST
Sequence conflicti496 – 4961A → G in AAA23752. (PubMed:1460045)Curated
Sequence conflicti555 – 5617GKVDNKA → QSGQ in AAA23752. (PubMed:1460045)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X70994 Genomic DNA. Translation: CAA50321.1.
L02649 Genomic DNA. Translation: AAA23752.1.
U00096 Genomic DNA. Translation: AAC74875.1.
AP009048 Genomic DNA. Translation: BAA15609.1.
PIRiE64941. S41589.
RefSeqiNP_416319.1. NC_000913.3.
YP_490066.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74875; AAC74875; b1805.
BAA15609; BAA15609; BAA15609.
GeneIDi12930156.
946327.
KEGGiecj:Y75_p1780.
eco:b1805.
PATRICi32118927. VBIEscCol129921_1881.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X70994 Genomic DNA. Translation: CAA50321.1 .
L02649 Genomic DNA. Translation: AAA23752.1 .
U00096 Genomic DNA. Translation: AAC74875.1 .
AP009048 Genomic DNA. Translation: BAA15609.1 .
PIRi E64941. S41589.
RefSeqi NP_416319.1. NC_000913.3.
YP_490066.1. NC_007779.1.

3D structure databases

ProteinModelPortali P69451.
SMRi P69451. Positions 20-551.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P69451. 6 interactions.
STRINGi 511145.b1805.

Protein family/group databases

TCDBi 4.C.1.1.4. the proposed fatty acid transporter (fat) family.

Proteomic databases

PaxDbi P69451.
PRIDEi P69451.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74875 ; AAC74875 ; b1805 .
BAA15609 ; BAA15609 ; BAA15609 .
GeneIDi 12930156.
946327.
KEGGi ecj:Y75_p1780.
eco:b1805.
PATRICi 32118927. VBIEscCol129921_1881.

Organism-specific databases

EchoBASEi EB1492.
EcoGenei EG11530. fadD.

Phylogenomic databases

eggNOGi COG0318.
HOGENOMi HOG000229983.
InParanoidi P69451.
KOi K01897.
OMAi VATRWHE.
OrthoDBi EOG6MH5BV.
PhylomeDBi P69451.

Enzyme and pathway databases

BioCyci EcoCyc:ACYLCOASYN-MONOMER.
ECOL316407:JW1794-MONOMER.
MetaCyc:ACYLCOASYN-MONOMER.
RETL1328306-WGS:GSTH-490-MONOMER.
SABIO-RK P69451.

Miscellaneous databases

PROi P69451.

Gene expression databases

Genevestigatori P69451.

Family and domain databases

InterProi IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view ]
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The fadD gene of Escherichia coli K12 is located close to rnd at 39.6 min of the chromosomal map and is a new member of the AMP-binding protein family."
    Fulda M., Heinz E., Wolter F.P.
    Mol. Gen. Genet. 242:241-249(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. "Cloning, sequencing, and expression of the fadD gene of Escherichia coli encoding acyl coenzyme A synthetase."
    Black P.N., Dirusso C.C., Metzger A.K., Heimert T.L.
    J. Biol. Chem. 267:25513-25520(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-10.
    Strain: K12.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  6. "Functional role of fatty acyl-coenzyme A synthetase in the transmembrane movement and activation of exogenous long-chain fatty acids. Amino acid residues within the ATP/AMP signature motif of Escherichia coli FadD are required for enzyme activity and fatty acid transport."
    Weimar J.D., DiRusso C.C., Delio R., Black P.N.
    J. Biol. Chem. 277:29369-29376(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF ATP-BINDING MOTIF, MUTAGENESIS OF TYR-213; THR-214; GLY-216; THR-217; GLY-219; LYS-222 AND GLU-361.

Entry informationi

Entry nameiLCFA_ECOLI
AccessioniPrimary (citable) accession number: P69451
Secondary accession number(s): P29212
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: October 29, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

Activity is the highest with fatty acid substrates of > 10 carbon atoms.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3