P69434 (PGAA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Poly-beta-1,6-N-acetyl-D-glucosamine export protein
Short name=PGA export protein
Short name=Poly-beta-1,6-GlcNAc export protein

Gene names

Name:	pgaA
Synonyms:	ycdS
Ordered Locus Names:	b1024, JW1010

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	807 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Exports the biofilm adhesin polysaccharide poly-beta-1,6-N-acetyl-D-glucosamine (PGA) across the outer membrane. The PGA transported seems to be partially N-deacetylated since N-deacetylation of PGA by PgaB is needed for PGA export through the PgaA porin. Ref.4 Ref.5 Ref.6 Required for the synthesis of the beta-1,6-GlcNAc polysaccharide (PGA or poly-GlcNAc) that seems to serve as a biofilm adhesin. Ref.4 Ref.5 Ref.6
Subcellular location	Cell outer membrane; Multi-pass membrane protein Probable Ref.5.
Induction	Levels of this protein are negatively controlled by the second messenger ppGpp (at protein level) at a post-transcriptional level. Increased levels of c-di-GMP lead to decreased levels of PgaA. Ref.6
Domain	Contains a predicted C-terminal beta-barrel porin domain and a N-terminal periplasmic superhelical domain containing tetratricopeptide repeats, which may mediate protein-protein interactions, perhaps with PgaB. Ref.5
Disruption phenotype	Deletion of pgaA does not prevent PGA synthesis but does block its export. The synthesized PGA is retained in the periplasm and accumulates at the cell poles. Disruption of the pga operon causes severe and persistent defects in the biofilm formation process. Ref.4 Ref.5
Sequence similarities	Contains 3 TPR repeats.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Cell outer membrane Membrane
Domain	Repeat Signal TPR repeat Transmembrane Transmembrane beta strand
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	biofilm formation Inferred from mutant phenotype Ref.4. Source: EcoCyc
Cellular_component	cell outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	substrate-specific transmembrane transporter activity Inferred from mutant phenotype Ref.5. Source: EcoCyc
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 807	781	Poly-beta-1,6-N-acetyl-D-glucosamine export protein		PRO_0000035694
Regions
	Repeat	98 – 131	34	TPR 1
	Repeat	165 – 198	34	TPR 2
	Repeat	279 – 311	33	TPR 3

Sequences

	Sequence		Length	Mass (Da)
	P69434 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: B20067C3D41723FD	FASTA	807	92,207
	10 20 30 40 50 60 MYSSSRKRCP KTKWALKLLT AAFLAASPAA KSAVNNAYDA LIIEARKGNT QPALSWFALK 70 80 90 100 110 120 SALSNNQIAD WLQIALWAGQ DKQVITVYNR YRHQQLPARG YAAVAVAYRN LQQWQNSLTL 130 140 150 160 170 180 WQKALSLEPQ NKDYQRGQIL TLADAGHYDT ALVKLKQLNS GAPDKANLLA EAYIYKLAGR 190 200 210 220 230 240 HQDELRAMTE SLPENASTQQ YPTEYVQALR NNQLAAAIDD ANLTPDIRAD IHAELVRLSF 250 260 270 280 290 300 MPTRSESERY AIADRALAQY AALEILWHDN PDRTAQYQRI QVDHLGALLT RDRYKDVISH 310 320 330 340 350 360 YQRLKKTGQI IPPWGQYWVA SAYLKDHQPK KAQSIMTELF YHKETIAPDL SDEELADLFY 370 380 390 400 410 420 SHLESENYPG ALTVTQHTIN TSPPFLRLMG TPTSIPNDTW LQGHSFLSTV AKYSNDLPQA 430 440 450 460 470 480 EMTARELAYN APGNQGLRID YASVLQARGW PRAAENELKK AEVIEPRNIN LEVEQAWTAL 490 500 510 520 530 540 TLQEWQQAAV LTHDVVEREP QDPGVVRLKR AVDVHNLAEL RIAGSTGIDA EGPDSGKHDV 550 560 570 580 590 600 DLTTIVYSPP LKDNWRGFAG FGYADGQFSE GKGIVRDWLA GVEWRSRNIW LEAEYAERVF 610 620 630 640 650 660 NHEHKPGARL SGWYDFNDNW RIGSQLERLS HRVPLRAMKN GVTGNSAQAY VRWYQNERRK 670 680 690 700 710 720 YGVSWAFTDF SDSNQRHEVS LEGQERIWSS PYLIVDFLPS LYYEQNTEHD TPYYNPIKTF 730 740 750 760 770 780 DIVPAFEASH LLWRSYENSW EQIFSAGVGA SWQKHYGTDV VTQLGYGQRI SWNDVIDAGA 790 800 TLRWEKRPYD GDREHNLYVE FDMTFRF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The pgaABCD locus of Escherichia coli promotes the synthesis of a polysaccharide adhesin required for biofilm formation." Wang X., Preston J.F. III, Romeo T. J. Bacteriol. 186:2724-2734(2004) [PubMed] [Europe PMC] [Abstract] Cited for: ROLE IN THE SYNTHESIS OF A BIOFILM POLYSACCHARIDE, OPERON STRUCTURE, DISRUPTION PHENOTYPE. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Roles of pgaABCD genes in synthesis, modification, and export of the Escherichia coli biofilm adhesin poly-beta-1,6-N-acetyl-D-glucosamine." Itoh Y., Rice J.D., Goller C., Pannuri A., Taylor J., Meisner J., Beveridge T.J., Preston J.F. III, Romeo T. J. Bacteriol. 190:3670-3680(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN PGA EXPORT, DOMAIN, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE. Strain: K12 / MG1655 / ATCC 47076.
[6]	"Second messenger signalling governs Escherichia coli biofilm induction upon ribosomal stress." Boehm A., Steiner S., Zaehringer F., Casanova A., Hamburger F., Ritz D., Keck W., Ackermann M., Schirmer T., Jenal U. Mol. Microbiol. 72:1500-1516(2009) [PubMed] [Europe PMC] [Abstract] Cited for: ROLE IN BIOFILM FORMATION, INDUCTION. Strain: K12 / MG1655 / AB400.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC74109.1. AP009048 Genomic DNA. Translation: BAA35806.1.
PIR	F64844.
RefSeq	NP_415543.1. NC_000913.2. YP_489295.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P69434.
ModBase	Search...
Protein-protein interaction databases
IntAct	P69434. 1 interaction.
STRING	511145.b1024.
Protein family/group databases
TCDB	1.B.55.1.1. poly acetyl glucosamine porin (PgaA) family.
Proteomic databases
PaxDb	P69434.
PRIDE	P69434.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74109; AAC74109; b1024. BAA35806; BAA35806; BAA35806.
GeneID	12934232. 945596.
KEGG	ecj:Y75_p0996. eco:b1024.
PATRIC	32117281. VBIEscCol129921_1064.
Organism-specific databases
EchoBASE	EB3625.
EcoGene	EG13865. pgaA.
Phylogenomic databases
eggNOG	NOG06511.
HOGENOM	HOG000118896.
KO	K11935.
OMA	LWRHYER.
ProtClustDB	PRK10049.
Enzyme and pathway databases
BioCyc	EcoCyc:G6531-MONOMER. ECOL316407:JW1010-MONOMER.
Gene expression databases
Genevestigator	P69434.
Family and domain databases
Gene3D	1.25.40.10. 2 hits.
InterPro	IPR023870. PGA_export_porin_PgaA. IPR013026. TPR-contain_dom. IPR011990. TPR-like_helical. IPR019734. TPR_repeat. [Graphical view]
TIGRFAMs	TIGR03939. PGA_TPR_OMP. 1 hit.
PROSITE	PS50005. TPR. 1 hit. PS50293. TPR_REGION. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGAA_ECOLI

Accession

Primary (citable) accession number: P69434
Secondary accession number(s): P75907

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	March 1, 2005
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program