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Protein

Poly-beta-1,6-N-acetyl-D-glucosamine export protein

Gene

pgaA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Exports the biofilm adhesin polysaccharide poly-beta-1,6-N-acetyl-D-glucosamine (PGA) across the outer membrane. The PGA transported seems to be partially N-deacetylated since N-deacetylation of PGA by PgaB is needed for PGA export through the PgaA porin.
Required for the synthesis of the beta-1,6-GlcNAc polysaccharide (PGA or poly-GlcNAc) that seems to serve as a biofilm adhesin.

GO - Molecular functioni

Keywordsi

Biological processTransport

Enzyme and pathway databases

BioCyciEcoCyc:G6531-MONOMER
MetaCyc:G6531-MONOMER
BRENDAi3.1.1.58 2026

Protein family/group databases

TCDBi1.B.55.1.1 the poly acetyl glucosamine porin (pgaa) family

Names & Taxonomyi

Protein namesi
Recommended name:
Poly-beta-1,6-N-acetyl-D-glucosamine export protein
Short name:
PGA export protein
Short name:
Poly-beta-1,6-GlcNAc export protein
Gene namesi
Name:pgaA
Synonyms:ycdS
Ordered Locus Names:b1024, JW1010
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13865 pgaA

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell outer membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deletion of pgaA does not prevent PGA synthesis but does block its export. The synthesized PGA is retained in the periplasm and accumulates at the cell poles. Disruption of the pga operon causes severe and persistent defects in the biofilm formation process.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_000003569427 – 807Poly-beta-1,6-N-acetyl-D-glucosamine export proteinAdd BLAST781

Proteomic databases

PaxDbiP69434
PRIDEiP69434

Expressioni

Inductioni

Levels of this protein are negatively controlled by the second messenger ppGpp (at protein level) at a post-transcriptional level. Increased levels of c-di-GMP lead to decreased levels of PgaA.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4263226, 121 interactors
IntActiP69434, 1 interactor
STRINGi316385.ECDH10B_1097

Structurei

Secondary structure

1807
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi518 – 530Combined sources13
Beta strandi540 – 547Combined sources8
Beta strandi551 – 565Combined sources15
Beta strandi574 – 586Combined sources13
Beta strandi589 – 599Combined sources11
Beta strandi604 – 617Combined sources14
Beta strandi620 – 629Combined sources10
Helixi638 – 640Combined sources3
Beta strandi644 – 670Combined sources27
Beta strandi675 – 689Combined sources15
Beta strandi691 – 697Combined sources7
Beta strandi700 – 705Combined sources6
Beta strandi717 – 723Combined sources7
Beta strandi725 – 735Combined sources11
Beta strandi738 – 753Combined sources16
Turni754 – 756Combined sources3
Beta strandi757 – 772Combined sources16
Turni773 – 775Combined sources3
Beta strandi776 – 807Combined sources32

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4Y25X-ray2.82A511-807[»]
ProteinModelPortaliP69434
SMRiP69434
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati98 – 131TPR 1Add BLAST34
Repeati165 – 198TPR 2Add BLAST34
Repeati279 – 311TPR 3Add BLAST33

Domaini

Contains a predicted C-terminal beta-barrel porin domain and a N-terminal periplasmic superhelical domain containing tetratricopeptide repeats, which may mediate protein-protein interactions, perhaps with PgaB.1 Publication

Keywords - Domaini

Repeat, Signal, TPR repeat, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4108TUY Bacteria
ENOG4111HEP LUCA
HOGENOMiHOG000118896
KOiK11935
OMAiWDKRPYD

Family and domain databases

Gene3Di1.25.40.10, 2 hits
InterProiView protein in InterPro
IPR023870 PGA_export_porin_PgaA
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR019734 TPR_repeat
SMARTiView protein in SMART
SM00028 TPR, 1 hit
SUPFAMiSSF48452 SSF48452, 2 hits
TIGRFAMsiTIGR03939 PGA_TPR_OMP, 1 hit
PROSITEiView protein in PROSITE
PS50005 TPR, 1 hit
PS50293 TPR_REGION, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P69434-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYSSSRKRCP KTKWALKLLT AAFLAASPAA KSAVNNAYDA LIIEARKGNT
60 70 80 90 100
QPALSWFALK SALSNNQIAD WLQIALWAGQ DKQVITVYNR YRHQQLPARG
110 120 130 140 150
YAAVAVAYRN LQQWQNSLTL WQKALSLEPQ NKDYQRGQIL TLADAGHYDT
160 170 180 190 200
ALVKLKQLNS GAPDKANLLA EAYIYKLAGR HQDELRAMTE SLPENASTQQ
210 220 230 240 250
YPTEYVQALR NNQLAAAIDD ANLTPDIRAD IHAELVRLSF MPTRSESERY
260 270 280 290 300
AIADRALAQY AALEILWHDN PDRTAQYQRI QVDHLGALLT RDRYKDVISH
310 320 330 340 350
YQRLKKTGQI IPPWGQYWVA SAYLKDHQPK KAQSIMTELF YHKETIAPDL
360 370 380 390 400
SDEELADLFY SHLESENYPG ALTVTQHTIN TSPPFLRLMG TPTSIPNDTW
410 420 430 440 450
LQGHSFLSTV AKYSNDLPQA EMTARELAYN APGNQGLRID YASVLQARGW
460 470 480 490 500
PRAAENELKK AEVIEPRNIN LEVEQAWTAL TLQEWQQAAV LTHDVVEREP
510 520 530 540 550
QDPGVVRLKR AVDVHNLAEL RIAGSTGIDA EGPDSGKHDV DLTTIVYSPP
560 570 580 590 600
LKDNWRGFAG FGYADGQFSE GKGIVRDWLA GVEWRSRNIW LEAEYAERVF
610 620 630 640 650
NHEHKPGARL SGWYDFNDNW RIGSQLERLS HRVPLRAMKN GVTGNSAQAY
660 670 680 690 700
VRWYQNERRK YGVSWAFTDF SDSNQRHEVS LEGQERIWSS PYLIVDFLPS
710 720 730 740 750
LYYEQNTEHD TPYYNPIKTF DIVPAFEASH LLWRSYENSW EQIFSAGVGA
760 770 780 790 800
SWQKHYGTDV VTQLGYGQRI SWNDVIDAGA TLRWEKRPYD GDREHNLYVE

FDMTFRF
Length:807
Mass (Da):92,207
Last modified:March 1, 2005 - v1
Checksum:iB20067C3D41723FD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74109.1
AP009048 Genomic DNA Translation: BAA35806.1
PIRiF64844
RefSeqiNP_415543.1, NC_000913.3
WP_000287458.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74109; AAC74109; b1024
BAA35806; BAA35806; BAA35806
GeneIDi945596
KEGGiecj:JW1010
eco:b1024
PATRICifig|1411691.4.peg.1245

Similar proteinsi

Entry informationi

Entry nameiPGAA_ECOLI
AccessioniPrimary (citable) accession number: P69434
Secondary accession number(s): P75907
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: March 28, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

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