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P69432

- PGAD_ECOLI

UniProt

P69432 - PGAD_ECOLI

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Protein

Biofilm PGA synthesis protein PgaD

Gene
pgaD, ycdP, b1021, JW1006
Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Required for the synthesis of poly-beta-1,6-N-acetyl-D-glucosamine (PGA), a biofilm adhesin polysaccharide. May assist the glycosyltransferase PgaC in the polymerization of PGA.3 Publications

GO - Molecular functioni

  1. protein binding Source: IntAct

GO - Biological processi

  1. cell adhesion involved in biofilm formation Source: EcoCyc
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:G6528-MONOMER.
ECOL316407:JW1006-MONOMER.
MetaCyc:G6528-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Biofilm PGA synthesis protein PgaD
Gene namesi
Name:pgaD
Synonyms:ycdP
Ordered Locus Names:b1021, JW1006
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG13862. pgaD.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2323Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei24 – 4421Helical; Reviewed predictionAdd
BLAST
Topological domaini45 – 539Periplasmic Reviewed prediction
Transmembranei54 – 7421Helical; Reviewed predictionAdd
BLAST
Topological domaini75 – 13763Cytoplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene do not synthesize PGA.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 137137Biofilm PGA synthesis protein PgaDPRO_0000058349Add
BLAST

Expressioni

Inductioni

Levels of this protein are positively controlled by the second messenger c-di-GMP (at protein level) at a post-transcriptional level. Increased levels of c-di-GMP lead to increased levels of PgaD.1 Publication

Gene expression databases

GenevestigatoriP69432.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
mnmEP255223EBI-562069,EBI-550986
pgaCP759052EBI-562069,EBI-561450

Protein-protein interaction databases

DIPiDIP-48114N.
IntActiP69432. 7 interactions.
STRINGi511145.b1021.

Structurei

3D structure databases

ProteinModelPortaliP69432.

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG263988.
HOGENOMiHOG000118885.
KOiK11937.
OMAiKKQQHHA.
OrthoDBiEOG6Q2SN7.

Family and domain databases

InterProiIPR023829. PGA_PgaD.
[Graphical view]
PfamiPF13994. PgaD. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03940. PGA_PgaD. 1 hit.

Sequencei

Sequence statusi: Complete.

P69432-1 [UniParc]FASTAAdd to Basket

« Hide

MNNLIITTRQ SPVRLLVDYV ATTILWTLFA LFIFLFAMDL LTGYYWQSEA    50
RSRLQFYFLL AVANAVVLIV WALYNKLRFQ KQQHHAAYQY TPQEYAESLA 100
IPDELYQQLQ KSHRMSVHFT SQGQIKMVVS EKALVRA 137
Length:137
Mass (Da):16,082
Last modified:March 1, 2005 - v1
Checksum:i574776B68F667D5D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00096 Genomic DNA. Translation: AAC74106.1.
AP009048 Genomic DNA. Translation: BAA35802.1.
PIRiC64844.
RefSeqiNP_415540.1. NC_000913.3.
YP_489292.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74106; AAC74106; b1021.
BAA35802; BAA35802; BAA35802.
GeneIDi12931061.
947503.
KEGGiecj:Y75_p0993.
eco:b1021.
PATRICi32117275. VBIEscCol129921_1061.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U00096 Genomic DNA. Translation: AAC74106.1 .
AP009048 Genomic DNA. Translation: BAA35802.1 .
PIRi C64844.
RefSeqi NP_415540.1. NC_000913.3.
YP_489292.1. NC_007779.1.

3D structure databases

ProteinModelPortali P69432.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-48114N.
IntActi P69432. 7 interactions.
STRINGi 511145.b1021.

Protocols and materials databases

DNASUi 947503.
Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74106 ; AAC74106 ; b1021 .
BAA35802 ; BAA35802 ; BAA35802 .
GeneIDi 12931061.
947503.
KEGGi ecj:Y75_p0993.
eco:b1021.
PATRICi 32117275. VBIEscCol129921_1061.

Organism-specific databases

EchoBASEi EB3622.
EcoGenei EG13862. pgaD.

Phylogenomic databases

eggNOGi NOG263988.
HOGENOMi HOG000118885.
KOi K11937.
OMAi KKQQHHA.
OrthoDBi EOG6Q2SN7.

Enzyme and pathway databases

BioCyci EcoCyc:G6528-MONOMER.
ECOL316407:JW1006-MONOMER.
MetaCyc:G6528-MONOMER.

Miscellaneous databases

PROi P69432.

Gene expression databases

Genevestigatori P69432.

Family and domain databases

InterProi IPR023829. PGA_PgaD.
[Graphical view ]
Pfami PF13994. PgaD. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR03940. PGA_PgaD. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "The pgaABCD locus of Escherichia coli promotes the synthesis of a polysaccharide adhesin required for biofilm formation."
    Wang X., Preston J.F. III, Romeo T.
    J. Bacteriol. 186:2724-2734(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: ROLE IN THE SYNTHESIS OF A BIOFILM POLYSACCHARIDE, OPERON STRUCTURE, DISRUPTION PHENOTYPE.
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.
  6. "Roles of pgaABCD genes in synthesis, modification, and export of the Escherichia coli biofilm adhesin poly-beta-1,6-N-acetyl-D-glucosamine."
    Itoh Y., Rice J.D., Goller C., Pannuri A., Taylor J., Meisner J., Beveridge T.J., Preston J.F. III, Romeo T.
    J. Bacteriol. 190:3670-3680(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN PGA SYNTHESIS, DISRUPTION PHENOTYPE.
    Strain: K12 / MG1655 / ATCC 47076.
  7. "Second messenger signalling governs Escherichia coli biofilm induction upon ribosomal stress."
    Boehm A., Steiner S., Zaehringer F., Casanova A., Hamburger F., Ritz D., Keck W., Ackermann M., Schirmer T., Jenal U.
    Mol. Microbiol. 72:1500-1516(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ROLE IN BIOFILM FORMATION, INDUCTION.
    Strain: K12 / MG1655 / AB400.

Entry informationi

Entry nameiPGAD_ECOLI
AccessioniPrimary (citable) accession number: P69432
Secondary accession number(s): P75904
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: June 11, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

External Data

Dasty 3

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