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Protein

Biofilm PGA synthesis protein PgaD

Gene

pgaD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for the synthesis of poly-beta-1,6-N-acetyl-D-glucosamine (PGA), a biofilm adhesin polysaccharide. May assist the glycosyltransferase PgaC in the polymerization of PGA.3 Publications

GO - Biological processi

  • cell adhesion involved in biofilm formation Source: EcoCyc

Enzyme and pathway databases

BioCyciEcoCyc:G6528-MONOMER
MetaCyc:G6528-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Biofilm PGA synthesis protein PgaD
Gene namesi
Name:pgaD
Synonyms:ycdP
Ordered Locus Names:b1021, JW1006
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13862 pgaD

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 23CytoplasmicSequence analysisAdd BLAST23
Transmembranei24 – 44HelicalSequence analysisAdd BLAST21
Topological domaini45 – 53PeriplasmicSequence analysis9
Transmembranei54 – 74HelicalSequence analysisAdd BLAST21
Topological domaini75 – 137CytoplasmicSequence analysisAdd BLAST63

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene do not synthesize PGA.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000583491 – 137Biofilm PGA synthesis protein PgaDAdd BLAST137

Proteomic databases

PaxDbiP69432
PRIDEiP69432

Expressioni

Inductioni

Levels of this protein are positively controlled by the second messenger c-di-GMP (at protein level) at a post-transcriptional level. Increased levels of c-di-GMP lead to increased levels of PgaD.1 Publication

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi4260055, 21 interactors
DIPiDIP-48114N
IntActiP69432, 7 interactors
MINTiP69432
STRINGi316385.ECDH10B_1094

Structurei

3D structure databases

ProteinModelPortaliP69432
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000118885
KOiK11937
OMAiKKQQHHA

Family and domain databases

InterProiView protein in InterPro
IPR023829 PGA_PgaD
PfamiView protein in Pfam
PF13994 PgaD, 1 hit
TIGRFAMsiTIGR03940 PGA_PgaD, 1 hit

Sequencei

Sequence statusi: Complete.

P69432-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNLIITTRQ SPVRLLVDYV ATTILWTLFA LFIFLFAMDL LTGYYWQSEA
60 70 80 90 100
RSRLQFYFLL AVANAVVLIV WALYNKLRFQ KQQHHAAYQY TPQEYAESLA
110 120 130
IPDELYQQLQ KSHRMSVHFT SQGQIKMVVS EKALVRA
Length:137
Mass (Da):16,082
Last modified:March 1, 2005 - v1
Checksum:i574776B68F667D5D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA Translation: AAC74106.1
AP009048 Genomic DNA Translation: BAA35802.1
PIRiC64844
RefSeqiNP_415540.1, NC_000913.3
WP_001061095.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC74106; AAC74106; b1021
BAA35802; BAA35802; BAA35802
GeneIDi947503
KEGGiecj:JW1006
eco:b1021
PATRICifig|1411691.4.peg.1248

Similar proteinsi

Entry informationi

Entry nameiPGAD_ECOLI
AccessioniPrimary (citable) accession number: P69432
Secondary accession number(s): P75904
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: March 28, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health