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Protein

Biofilm PGA synthesis protein PgaD

Gene

pgaD

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Required for the synthesis of poly-beta-1,6-N-acetyl-D-glucosamine (PGA), a biofilm adhesin polysaccharide. May assist the glycosyltransferase PgaC in the polymerization of PGA.3 Publications

GO - Biological processi

  • cell adhesion involved in biofilm formation Source: EcoCyc
Complete GO annotation...

Enzyme and pathway databases

BioCyciEcoCyc:G6528-MONOMER.
ECOL316407:JW1006-MONOMER.
MetaCyc:G6528-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Biofilm PGA synthesis protein PgaD
Gene namesi
Name:pgaD
Synonyms:ycdP
Ordered Locus Names:b1021, JW1006
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13862. pgaD.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2323CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei24 – 4421HelicalSequence AnalysisAdd
BLAST
Topological domaini45 – 539PeriplasmicSequence Analysis
Transmembranei54 – 7421HelicalSequence AnalysisAdd
BLAST
Topological domaini75 – 13763CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Cells lacking this gene do not synthesize PGA.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 137137Biofilm PGA synthesis protein PgaDPRO_0000058349Add
BLAST

Expressioni

Inductioni

Levels of this protein are positively controlled by the second messenger c-di-GMP (at protein level) at a post-transcriptional level. Increased levels of c-di-GMP lead to increased levels of PgaD.1 Publication

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
mnmEP255223EBI-562069,EBI-550986
pgaCP759052EBI-562069,EBI-561450

Protein-protein interaction databases

DIPiDIP-48114N.
IntActiP69432. 7 interactions.
STRINGi511145.b1021.

Structurei

3D structure databases

ProteinModelPortaliP69432.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG263988.
HOGENOMiHOG000118885.
KOiK11937.
OMAiKKQQHHA.
OrthoDBiEOG6Q2SN7.

Family and domain databases

InterProiIPR023829. PGA_PgaD.
[Graphical view]
PfamiPF13994. PgaD. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03940. PGA_PgaD. 1 hit.

Sequencei

Sequence statusi: Complete.

P69432-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNLIITTRQ SPVRLLVDYV ATTILWTLFA LFIFLFAMDL LTGYYWQSEA
60 70 80 90 100
RSRLQFYFLL AVANAVVLIV WALYNKLRFQ KQQHHAAYQY TPQEYAESLA
110 120 130
IPDELYQQLQ KSHRMSVHFT SQGQIKMVVS EKALVRA
Length:137
Mass (Da):16,082
Last modified:March 1, 2005 - v1
Checksum:i574776B68F667D5D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74106.1.
AP009048 Genomic DNA. Translation: BAA35802.1.
PIRiC64844.
RefSeqiNP_415540.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC74106; AAC74106; b1021.
BAA35802; BAA35802; BAA35802.
GeneIDi947503.
KEGGiecj:Y75_p0993.
eco:b1021.
PATRICi32117275. VBIEscCol129921_1061.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74106.1.
AP009048 Genomic DNA. Translation: BAA35802.1.
PIRiC64844.
RefSeqiNP_415540.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP69432.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48114N.
IntActiP69432. 7 interactions.
STRINGi511145.b1021.

Protocols and materials databases

DNASUi947503.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74106; AAC74106; b1021.
BAA35802; BAA35802; BAA35802.
GeneIDi947503.
KEGGiecj:Y75_p0993.
eco:b1021.
PATRICi32117275. VBIEscCol129921_1061.

Organism-specific databases

EchoBASEiEB3622.
EcoGeneiEG13862. pgaD.

Phylogenomic databases

eggNOGiNOG263988.
HOGENOMiHOG000118885.
KOiK11937.
OMAiKKQQHHA.
OrthoDBiEOG6Q2SN7.

Enzyme and pathway databases

BioCyciEcoCyc:G6528-MONOMER.
ECOL316407:JW1006-MONOMER.
MetaCyc:G6528-MONOMER.

Miscellaneous databases

PROiP69432.

Family and domain databases

InterProiIPR023829. PGA_PgaD.
[Graphical view]
PfamiPF13994. PgaD. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03940. PGA_PgaD. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "The pgaABCD locus of Escherichia coli promotes the synthesis of a polysaccharide adhesin required for biofilm formation."
    Wang X., Preston J.F. III, Romeo T.
    J. Bacteriol. 186:2724-2734(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: ROLE IN THE SYNTHESIS OF A BIOFILM POLYSACCHARIDE, OPERON STRUCTURE, DISRUPTION PHENOTYPE.
    Strain: K12 / MG1655 / ATCC 47076.
  5. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION.
    Strain: K12 / MG1655 / ATCC 47076.
  6. "Roles of pgaABCD genes in synthesis, modification, and export of the Escherichia coli biofilm adhesin poly-beta-1,6-N-acetyl-D-glucosamine."
    Itoh Y., Rice J.D., Goller C., Pannuri A., Taylor J., Meisner J., Beveridge T.J., Preston J.F. III, Romeo T.
    J. Bacteriol. 190:3670-3680(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN PGA SYNTHESIS, DISRUPTION PHENOTYPE.
    Strain: K12 / MG1655 / ATCC 47076.
  7. "Second messenger signalling governs Escherichia coli biofilm induction upon ribosomal stress."
    Boehm A., Steiner S., Zaehringer F., Casanova A., Hamburger F., Ritz D., Keck W., Ackermann M., Schirmer T., Jenal U.
    Mol. Microbiol. 72:1500-1516(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: ROLE IN BIOFILM FORMATION, INDUCTION.
    Strain: K12 / MG1655 / AB400.

Entry informationi

Entry nameiPGAD_ECOLI
AccessioniPrimary (citable) accession number: P69432
Secondary accession number(s): P75904
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: June 24, 2015
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.