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P69432 (PGAD_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biofilm PGA synthesis protein PgaD
Gene names
Name:pgaD
Synonyms:ycdP
Ordered Locus Names:b1021, JW1006
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length137 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for the synthesis of poly-beta-1,6-N-acetyl-D-glucosamine (PGA), a biofilm adhesin polysaccharide. May assist the glycosyltransferase PgaC in the polymerization of PGA. Ref.4 Ref.6 Ref.7

Subcellular location

Cell inner membrane; Multi-pass membrane protein.

Induction

Levels of this protein are positively controlled by the second messenger c-di-GMP (at protein level) at a post-transcriptional level. Increased levels of c-di-GMP lead to increased levels of PgaD. Ref.7

Disruption phenotype

Cells lacking this gene do not synthesize PGA. Ref.4 Ref.6

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 137137Biofilm PGA synthesis protein PgaD
PRO_0000058349

Regions

Topological domain1 – 2323Cytoplasmic Potential
Transmembrane24 – 4421Helical; Potential
Topological domain45 – 539Periplasmic Potential
Transmembrane54 – 7421Helical; Potential
Topological domain75 – 13763Cytoplasmic Potential

Sequences

Sequence LengthMass (Da)Tools
P69432 [UniParc].

Last modified March 1, 2005. Version 1.
Checksum: 574776B68F667D5D

FASTA13716,082
        10         20         30         40         50         60 
MNNLIITTRQ SPVRLLVDYV ATTILWTLFA LFIFLFAMDL LTGYYWQSEA RSRLQFYFLL 

        70         80         90        100        110        120 
AVANAVVLIV WALYNKLRFQ KQQHHAAYQY TPQEYAESLA IPDELYQQLQ KSHRMSVHFT 

       130 
SQGQIKMVVS EKALVRA 

« Hide

References

« Hide 'large scale' references
[1]"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map."
Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. expand/collapse author list , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T.
DNA Res. 3:137-155(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"The pgaABCD locus of Escherichia coli promotes the synthesis of a polysaccharide adhesin required for biofilm formation."
Wang X., Preston J.F. III, Romeo T.
J. Bacteriol. 186:2724-2734(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: ROLE IN THE SYNTHESIS OF A BIOFILM POLYSACCHARIDE, OPERON STRUCTURE, DISRUPTION PHENOTYPE.
Strain: K12 / MG1655 / ATCC 47076.
[5]"Global topology analysis of the Escherichia coli inner membrane proteome."
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION.
Strain: K12 / MG1655 / ATCC 47076.
[6]"Roles of pgaABCD genes in synthesis, modification, and export of the Escherichia coli biofilm adhesin poly-beta-1,6-N-acetyl-D-glucosamine."
Itoh Y., Rice J.D., Goller C., Pannuri A., Taylor J., Meisner J., Beveridge T.J., Preston J.F. III, Romeo T.
J. Bacteriol. 190:3670-3680(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION IN PGA SYNTHESIS, DISRUPTION PHENOTYPE.
Strain: K12 / MG1655 / ATCC 47076.
[7]"Second messenger signalling governs Escherichia coli biofilm induction upon ribosomal stress."
Boehm A., Steiner S., Zaehringer F., Casanova A., Hamburger F., Ritz D., Keck W., Ackermann M., Schirmer T., Jenal U.
Mol. Microbiol. 72:1500-1516(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: ROLE IN BIOFILM FORMATION, INDUCTION.
Strain: K12 / MG1655 / AB400.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00096 Genomic DNA. Translation: AAC74106.1.
AP009048 Genomic DNA. Translation: BAA35802.1.
PIRC64844.
RefSeqNP_415540.1. NC_000913.3.
YP_489292.1. NC_007779.1.

3D structure databases

ProteinModelPortalP69432.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-48114N.
IntActP69432. 7 interactions.
STRING511145.b1021.

Protocols and materials databases

DNASU947503.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74106; AAC74106; b1021.
BAA35802; BAA35802; BAA35802.
GeneID12931061.
947503.
KEGGecj:Y75_p0993.
eco:b1021.
PATRIC32117275. VBIEscCol129921_1061.

Organism-specific databases

EchoBASEEB3622.
EcoGeneEG13862. pgaD.

Phylogenomic databases

eggNOGNOG263988.
HOGENOMHOG000118885.
KOK11937.
OMAKKQQHHA.
OrthoDBEOG6Q2SN7.
ProtClustDBPRK14585.

Enzyme and pathway databases

BioCycEcoCyc:G6528-MONOMER.
ECOL316407:JW1006-MONOMER.
MetaCyc:G6528-MONOMER.

Gene expression databases

GenevestigatorP69432.

Family and domain databases

InterProIPR023829. PGA_PgaD.
[Graphical view]
PfamPF13994. PgaD. 1 hit.
[Graphical view]
TIGRFAMsTIGR03940. PGA_PgaD. 1 hit.
ProtoNetSearch...

Other

PROP69432.

Entry information

Entry namePGAD_ECOLI
AccessionPrimary (citable) accession number: P69432
Secondary accession number(s): P75904
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: April 16, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene