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P69432

- PGAD_ECOLI

UniProt

P69432 - PGAD_ECOLI

Protein

Biofilm PGA synthesis protein PgaD

Gene

pgaD

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Required for the synthesis of poly-beta-1,6-N-acetyl-D-glucosamine (PGA), a biofilm adhesin polysaccharide. May assist the glycosyltransferase PgaC in the polymerization of PGA.3 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct

    GO - Biological processi

    1. cell adhesion involved in biofilm formation Source: EcoCyc

    Enzyme and pathway databases

    BioCyciEcoCyc:G6528-MONOMER.
    ECOL316407:JW1006-MONOMER.
    MetaCyc:G6528-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biofilm PGA synthesis protein PgaD
    Gene namesi
    Name:pgaD
    Synonyms:ycdP
    Ordered Locus Names:b1021, JW1006
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG13862. pgaD.

    Subcellular locationi

    Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    Cells lacking this gene do not synthesize PGA.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 137137Biofilm PGA synthesis protein PgaDPRO_0000058349Add
    BLAST

    Expressioni

    Inductioni

    Levels of this protein are positively controlled by the second messenger c-di-GMP (at protein level) at a post-transcriptional level. Increased levels of c-di-GMP lead to increased levels of PgaD.1 Publication

    Gene expression databases

    GenevestigatoriP69432.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    mnmEP255223EBI-562069,EBI-550986
    pgaCP759052EBI-562069,EBI-561450

    Protein-protein interaction databases

    DIPiDIP-48114N.
    IntActiP69432. 7 interactions.
    STRINGi511145.b1021.

    Structurei

    3D structure databases

    ProteinModelPortaliP69432.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2323CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini45 – 539PeriplasmicSequence Analysis
    Topological domaini75 – 13763CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei24 – 4421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei54 – 7421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG263988.
    HOGENOMiHOG000118885.
    KOiK11937.
    OMAiKKQQHHA.
    OrthoDBiEOG6Q2SN7.

    Family and domain databases

    InterProiIPR023829. PGA_PgaD.
    [Graphical view]
    PfamiPF13994. PgaD. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR03940. PGA_PgaD. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P69432-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNNLIITTRQ SPVRLLVDYV ATTILWTLFA LFIFLFAMDL LTGYYWQSEA    50
    RSRLQFYFLL AVANAVVLIV WALYNKLRFQ KQQHHAAYQY TPQEYAESLA 100
    IPDELYQQLQ KSHRMSVHFT SQGQIKMVVS EKALVRA 137
    Length:137
    Mass (Da):16,082
    Last modified:March 1, 2005 - v1
    Checksum:i574776B68F667D5D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC74106.1.
    AP009048 Genomic DNA. Translation: BAA35802.1.
    PIRiC64844.
    RefSeqiNP_415540.1. NC_000913.3.
    YP_489292.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74106; AAC74106; b1021.
    BAA35802; BAA35802; BAA35802.
    GeneIDi12931061.
    947503.
    KEGGiecj:Y75_p0993.
    eco:b1021.
    PATRICi32117275. VBIEscCol129921_1061.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC74106.1 .
    AP009048 Genomic DNA. Translation: BAA35802.1 .
    PIRi C64844.
    RefSeqi NP_415540.1. NC_000913.3.
    YP_489292.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P69432.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-48114N.
    IntActi P69432. 7 interactions.
    STRINGi 511145.b1021.

    Protocols and materials databases

    DNASUi 947503.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74106 ; AAC74106 ; b1021 .
    BAA35802 ; BAA35802 ; BAA35802 .
    GeneIDi 12931061.
    947503.
    KEGGi ecj:Y75_p0993.
    eco:b1021.
    PATRICi 32117275. VBIEscCol129921_1061.

    Organism-specific databases

    EchoBASEi EB3622.
    EcoGenei EG13862. pgaD.

    Phylogenomic databases

    eggNOGi NOG263988.
    HOGENOMi HOG000118885.
    KOi K11937.
    OMAi KKQQHHA.
    OrthoDBi EOG6Q2SN7.

    Enzyme and pathway databases

    BioCyci EcoCyc:G6528-MONOMER.
    ECOL316407:JW1006-MONOMER.
    MetaCyc:G6528-MONOMER.

    Miscellaneous databases

    PROi P69432.

    Gene expression databases

    Genevestigatori P69432.

    Family and domain databases

    InterProi IPR023829. PGA_PgaD.
    [Graphical view ]
    Pfami PF13994. PgaD. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR03940. PGA_PgaD. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "The pgaABCD locus of Escherichia coli promotes the synthesis of a polysaccharide adhesin required for biofilm formation."
      Wang X., Preston J.F. III, Romeo T.
      J. Bacteriol. 186:2724-2734(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: ROLE IN THE SYNTHESIS OF A BIOFILM POLYSACCHARIDE, OPERON STRUCTURE, DISRUPTION PHENOTYPE.
      Strain: K12 / MG1655 / ATCC 47076.
    5. "Global topology analysis of the Escherichia coli inner membrane proteome."
      Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
      Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION.
      Strain: K12 / MG1655 / ATCC 47076.
    6. "Roles of pgaABCD genes in synthesis, modification, and export of the Escherichia coli biofilm adhesin poly-beta-1,6-N-acetyl-D-glucosamine."
      Itoh Y., Rice J.D., Goller C., Pannuri A., Taylor J., Meisner J., Beveridge T.J., Preston J.F. III, Romeo T.
      J. Bacteriol. 190:3670-3680(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN PGA SYNTHESIS, DISRUPTION PHENOTYPE.
      Strain: K12 / MG1655 / ATCC 47076.
    7. "Second messenger signalling governs Escherichia coli biofilm induction upon ribosomal stress."
      Boehm A., Steiner S., Zaehringer F., Casanova A., Hamburger F., Ritz D., Keck W., Ackermann M., Schirmer T., Jenal U.
      Mol. Microbiol. 72:1500-1516(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: ROLE IN BIOFILM FORMATION, INDUCTION.
      Strain: K12 / MG1655 / AB400.

    Entry informationi

    Entry nameiPGAD_ECOLI
    AccessioniPrimary (citable) accession number: P69432
    Secondary accession number(s): P75904
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 1, 2005
    Last sequence update: March 1, 2005
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene

    External Data

    Dasty 3