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Protein

Sec-independent protein translocase protein TatA

Gene

tatA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. TatA could form the protein-conducting channel of the Tat system.UniRule annotation2 Publications

GO - Molecular functioni

  • identical protein binding Source: EcoCyc
  • protein transporter activity Source: EcoliWiki
  • proton motive force dependent protein transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • intracellular protein transmembrane transport Source: EcoCyc
  • protein secretion Source: InterPro
  • protein transport by the Tat complex Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciEcoCyc:TATA.
ECOL316407:JW3813-MONOMER.
MetaCyc:TATA.

Protein family/group databases

TCDBi2.A.64.1.1. the twin arginine targeting (tat) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Sec-independent protein translocase protein TatAUniRule annotation
Gene namesi
Name:tatAUniRule annotation
Synonyms:mttA1, yigT
Ordered Locus Names:b3836, JW3813
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11478. tatA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1 – 2121HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

  • cytosol Source: EcoCyc
  • integral component of membrane Source: EcoliWiki
  • integral component of plasma membrane Source: EcoCyc
  • TAT protein transport complex Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Disruption of tatA affects the correct localization of multiple enzymes whose precursors bear twin arginine transfer peptides. Export is completely blocked when both tatA and tatE are inactivated.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi39 – 391F → A: Homooligomerizes more efficiently than wt, blocks translocation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 8989Sec-independent protein translocase protein TatAPRO_0000097934Add
BLAST

Proteomic databases

EPDiP69428.
PaxDbiP69428.
PRIDEiP69428.

Expressioni

Inductioni

Constitutively expressed. TatA is the most highly expressed of the tat genes.1 Publication

Interactioni

Subunit structurei

The Tat system comprises two distinct complexes: a TatABC complex, containing multiple copies of TatA, TatB and TatC subunits, and a separate TatA complex, containing only TatA subunits. Substrates initially bind to the TatABC complex, which probably triggers association of the separate TatA complex to form the active translocon. A complex containing only TatA and TatB has also been identified. It could be either an assembly intermediate or a disassembly intermediate generated during translocation activity. Each of TatA, TatB and TatC are able to interact in pairs without the third partner; TatA also forms homooligomers.UniRule annotation5 Publications

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4263055. 355 interactions.
DIPiDIP-10958N.
IntActiP69428. 2 interactions.
MINTiMINT-8083033.
STRINGi511145.b3836.

Structurei

Secondary structure

1
89
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 53Combined sources
Helixi6 – 3227Combined sources
Helixi34 – 429Combined sources
Helixi43 – 464Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LZRNMR-A1-49[»]
2LZSNMR-A/B/C/D/E/F/G/H/I1-49[»]
2MN6NMR-A/B1-89[»]
2MN7NMR-A1-89[»]
ProteinModelPortaliP69428.
SMRiP69428. Positions 1-48.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TatA/E family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105WKS. Bacteria.
COG1826. LUCA.
HOGENOMiHOG000245363.
InParanoidiP69428.
KOiK03116.
OMAiKEDAKRH.
PhylomeDBiP69428.

Family and domain databases

HAMAPiMF_00236. TatA_E. 1 hit.
InterProiIPR003369. TatA/B/E.
IPR006312. TatA/E.
[Graphical view]
PfamiPF02416. MttA_Hcf106. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01411. tatAE. 1 hit.

Sequencei

Sequence statusi: Complete.

P69428-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGGISIWQLL IIAVIVVLLF GTKKLGSIGS DLGASIKGFK KAMSDDEPKQ
60 70 80
DKTSQDADFT AKTIADKQAD TNQEQAKTED AKRHDKEQV
Length:89
Mass (Da):9,664
Last modified:March 1, 2005 - v1
Checksum:i4874F393EFFADC1B
GO

Sequence cautioni

The sequence AAA67633 differs from that shown. Reason: Frameshift at positions 11, 82 and 88. Curated
The sequence AAC19240 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005830 Genomic DNA. Translation: CAA06724.1.
AF067848 Genomic DNA. Translation: AAC19240.1. Different initiation.
M87049 Genomic DNA. Translation: AAA67633.1. Frameshift.
U00096 Genomic DNA. Translation: AAC76839.2.
AP009048 Genomic DNA. Translation: BAE77465.1.
PIRiE65188.
S30727.
RefSeqiNP_418280.4. NC_000913.3.
WP_001295260.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76839; AAC76839; b3836.
BAE77465; BAE77465; BAE77465.
GeneIDi948321.
KEGGiecj:JW3813.
eco:b3836.
PATRICi32123173. VBIEscCol129921_3952.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005830 Genomic DNA. Translation: CAA06724.1.
AF067848 Genomic DNA. Translation: AAC19240.1. Different initiation.
M87049 Genomic DNA. Translation: AAA67633.1. Frameshift.
U00096 Genomic DNA. Translation: AAC76839.2.
AP009048 Genomic DNA. Translation: BAE77465.1.
PIRiE65188.
S30727.
RefSeqiNP_418280.4. NC_000913.3.
WP_001295260.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LZRNMR-A1-49[»]
2LZSNMR-A/B/C/D/E/F/G/H/I1-49[»]
2MN6NMR-A/B1-89[»]
2MN7NMR-A1-89[»]
ProteinModelPortaliP69428.
SMRiP69428. Positions 1-48.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263055. 355 interactions.
DIPiDIP-10958N.
IntActiP69428. 2 interactions.
MINTiMINT-8083033.
STRINGi511145.b3836.

Protein family/group databases

TCDBi2.A.64.1.1. the twin arginine targeting (tat) family.

Proteomic databases

EPDiP69428.
PaxDbiP69428.
PRIDEiP69428.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76839; AAC76839; b3836.
BAE77465; BAE77465; BAE77465.
GeneIDi948321.
KEGGiecj:JW3813.
eco:b3836.
PATRICi32123173. VBIEscCol129921_3952.

Organism-specific databases

EcoGeneiEG11478. tatA.

Phylogenomic databases

eggNOGiENOG4105WKS. Bacteria.
COG1826. LUCA.
HOGENOMiHOG000245363.
InParanoidiP69428.
KOiK03116.
OMAiKEDAKRH.
PhylomeDBiP69428.

Enzyme and pathway databases

BioCyciEcoCyc:TATA.
ECOL316407:JW3813-MONOMER.
MetaCyc:TATA.

Miscellaneous databases

PROiP69428.

Family and domain databases

HAMAPiMF_00236. TatA_E. 1 hit.
InterProiIPR003369. TatA/B/E.
IPR006312. TatA/E.
[Graphical view]
PfamiPF02416. MttA_Hcf106. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01411. tatAE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTATA_ECOLI
AccessioniPrimary (citable) accession number: P69428
Secondary accession number(s): O65938, P27856, Q2M8E1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: September 7, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

TatA forms a transmembrane channel with a lid structure on the cytoplasmic side. The number of TatA protomers can vary so that the channel can adopt different diameters to accommodate substrates of various sizes. This enables TatA to pack tightly around substrate and prevents ion leakage during transport.
Absence of TatA from the TatBC complex leads to the instability of the complex and the specific breakdown of TatB.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.