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Protein

Sec-independent protein translocase protein TatC

Gene

tatC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of the twin-arginine translocation (Tat) system that transports large folded proteins containing a characteristic twin-arginine motif in their signal peptide across membranes. Together with TatB, TatC is part of a receptor directly interacting with Tat signal peptides.UniRule annotation5 Publications

GO - Molecular functioni

  • identical protein binding Source: EcoCyc
  • protein transporter activity Source: EcoliWiki
  • proton motive force dependent protein transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • intracellular protein transmembrane transport Source: EcoCyc
  • protein transport by the Tat complex Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Protein transport, Translocation, Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG11479-MONOMER.
ECOL316407:JW3815-MONOMER.
MetaCyc:EG11479-MONOMER.

Protein family/group databases

TCDBi2.A.64.1.1. the twin arginine targeting (tat) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Sec-independent protein translocase protein TatCUniRule annotation
Gene namesi
Name:tatCUniRule annotation
Synonyms:mttB, yigU, yigV
Ordered Locus Names:b3839, JW3815
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11479. tatC.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 23CytoplasmicSequence analysisAdd BLAST22
Transmembranei24 – 44HelicalUniRule annotationAdd BLAST21
Topological domaini45 – 75PeriplasmicSequence analysisAdd BLAST31
Transmembranei76 – 96HelicalUniRule annotationAdd BLAST21
Topological domaini97 – 115CytoplasmicSequence analysisAdd BLAST19
Transmembranei116 – 136HelicalUniRule annotationAdd BLAST21
Topological domaini137 – 156PeriplasmicSequence analysisAdd BLAST20
Transmembranei157 – 177HelicalUniRule annotationAdd BLAST21
Topological domaini178 – 192CytoplasmicSequence analysisAdd BLAST15
Transmembranei193 – 210HelicalUniRule annotationAdd BLAST18
Topological domaini211PeriplasmicSequence analysis1
Transmembranei212 – 232HelicalUniRule annotationAdd BLAST21
Topological domaini233 – 258CytoplasmicSequence analysisAdd BLAST26

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • intracellular Source: GOC
  • membrane Source: EcoliWiki
  • plasma membrane Source: EcoCyc
  • TAT protein transport complex Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Disruption blocks the export of at least five twin-arginine-containing precursor proteins that are predicted to bind redox cofactors, and hence fold prior to translocation. Disruption does not affect the Sec pathway.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi17R → A: No anaerobic growth and no TorA export. 1 Publication1
Mutagenesisi20 – 22Missing : No anaerobic growth and 75% decrease in TorA export. 1 Publication3
Mutagenesisi20L → A: 25% decrease in anaerobic growth and 75% decrease in TorA export. 1 Publication1
Mutagenesisi94F → A or L: Loss of function. 1 Publication1
Mutagenesisi94F → A: Export of TorA restored; when associated with A-211. 1 Publication1
Mutagenesisi103E → A, D or R: Loss of function. 1 Publication1
Mutagenesisi103E → A: Export of TorA restored; when associated with A-211. 1 Publication1
Mutagenesisi103E → Q: Severely retard but does not abolish activity. 1 Publication1
Mutagenesisi211D → A: Export activity of TorA blocked. Export of TorA restored; when associated with A-94 or A-103. 1 Publication1
Mutagenesisi211D → E or N: Decrease in TorA export activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00000980832 – 258Sec-independent protein translocase protein TatCAdd BLAST257

Proteomic databases

PaxDbiP69423.
PRIDEiP69423.

Expressioni

Inductioni

Constitutively expressed.1 Publication

Interactioni

Subunit structurei

The Tat system comprises two distinct complexes: a TatABC complex, containing multiple copies of TatA, TatB and TatC subunits, and a separate TatA complex, containing only TatA subunits. Substrates initially bind to the TatABC complex, which probably triggers association of the separate TatA complex to form the active translocon. TatC can form a distinct, stable, multimeric complex independent of TatA and TatB. Each of TatA, TatB and TatC are able to interact in pairs without the third partner. Interacts with the signal sequence of DmsA and DmsD.UniRule annotation6 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
tatBP694253EBI-4411641,EBI-4411577

GO - Molecular functioni

  • identical protein binding Source: EcoCyc

Protein-protein interaction databases

BioGridi4259567. 343 interactors.
DIPiDIP-58537N.
IntActiP69423. 4 interactors.
MINTiMINT-8083961.
STRINGi511145.b3839.

Structurei

3D structure databases

ProteinModelPortaliP69423.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TatC family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105DCX. Bacteria.
COG0805. LUCA.
HOGENOMiHOG000245379.
InParanoidiP69423.
KOiK03118.
OMAiATQIYKF.
PhylomeDBiP69423.

Family and domain databases

HAMAPiMF_00902. TatC. 1 hit.
InterProiIPR019820. Sec-indep_translocase_CS.
IPR002033. TatC.
[Graphical view]
PANTHERiPTHR30371:SF0. PTHR30371:SF0. 1 hit.
PfamiPF00902. TatC. 1 hit.
[Graphical view]
PRINTSiPR01840. TATCFAMILY.
TIGRFAMsiTIGR00945. tatC. 1 hit.
PROSITEiPS01218. TATC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P69423-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVEDTQPLI THLIELRKRL LNCIIAVIVI FLCLVYFAND IYHLVSAPLI
60 70 80 90 100
KQLPQGSTMI ATDVASPFFT PIKLTFMVSL ILSAPVILYQ VWAFIAPALY
110 120 130 140 150
KHERRLVVPL LVSSSLLFYI GMAFAYFVVF PLAFGFLANT APEGVQVSTD
160 170 180 190 200
IASYLSFVMA LFMAFGVSFE VPVAIVLLCW MGITSPEDLR KKRPYVLVGA
210 220 230 240 250
FVVGMLLTPP DVFSQTLLAI PMYCLFEIGV FFSRFYVGKG RNREEENDAE

AESEKTEE
Length:258
Mass (Da):28,876
Last modified:January 23, 2007 - v2
Checksum:iD2383F85AF62A81C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005830 Genomic DNA. Translation: CAA06726.1.
AF067848 Genomic DNA. Translation: AAC19242.1.
M87049 Genomic DNA. Translation: AAA67634.1. Frameshift.
M87049 Genomic DNA. Translation: AAA67635.1. Frameshift.
U00096 Genomic DNA. Translation: AAC76842.1.
AP009048 Genomic DNA. Translation: BAE77463.1.
PIRiH65188.
S30728.
RefSeqiNP_418282.1. NC_000913.3.
WP_000109943.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76842; AAC76842; b3839.
BAE77463; BAE77463; BAE77463.
GeneIDi948328.
KEGGiecj:JW3815.
eco:b3839.
PATRICi32123177. VBIEscCol129921_3954.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005830 Genomic DNA. Translation: CAA06726.1.
AF067848 Genomic DNA. Translation: AAC19242.1.
M87049 Genomic DNA. Translation: AAA67634.1. Frameshift.
M87049 Genomic DNA. Translation: AAA67635.1. Frameshift.
U00096 Genomic DNA. Translation: AAC76842.1.
AP009048 Genomic DNA. Translation: BAE77463.1.
PIRiH65188.
S30728.
RefSeqiNP_418282.1. NC_000913.3.
WP_000109943.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP69423.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259567. 343 interactors.
DIPiDIP-58537N.
IntActiP69423. 4 interactors.
MINTiMINT-8083961.
STRINGi511145.b3839.

Protein family/group databases

TCDBi2.A.64.1.1. the twin arginine targeting (tat) family.

Proteomic databases

PaxDbiP69423.
PRIDEiP69423.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76842; AAC76842; b3839.
BAE77463; BAE77463; BAE77463.
GeneIDi948328.
KEGGiecj:JW3815.
eco:b3839.
PATRICi32123177. VBIEscCol129921_3954.

Organism-specific databases

EchoBASEiEB1445.
EcoGeneiEG11479. tatC.

Phylogenomic databases

eggNOGiENOG4105DCX. Bacteria.
COG0805. LUCA.
HOGENOMiHOG000245379.
InParanoidiP69423.
KOiK03118.
OMAiATQIYKF.
PhylomeDBiP69423.

Enzyme and pathway databases

BioCyciEcoCyc:EG11479-MONOMER.
ECOL316407:JW3815-MONOMER.
MetaCyc:EG11479-MONOMER.

Miscellaneous databases

PROiP69423.

Family and domain databases

HAMAPiMF_00902. TatC. 1 hit.
InterProiIPR019820. Sec-indep_translocase_CS.
IPR002033. TatC.
[Graphical view]
PANTHERiPTHR30371:SF0. PTHR30371:SF0. 1 hit.
PfamiPF00902. TatC. 1 hit.
[Graphical view]
PRINTSiPR01840. TATCFAMILY.
TIGRFAMsiTIGR00945. tatC. 1 hit.
PROSITEiPS01218. TATC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTATC_ECOLI
AccessioniPrimary (citable) accession number: P69423
Secondary accession number(s): P27857
, P27858, P76765, P76766, Q2M8E3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 97 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.