P69405 (RCSA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Colanic acid capsular biosynthesis activation protein A

Gene names

Name:	rcsA
Ordered Locus Names:	b1951, JW1935

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia › Escherichia coli

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Positive regulator of capsular polysaccharide synthesis. RcsA and RcsB form a complex to promote transcription of the genes for capsule synthesis.
Post-translational modification	Degradation of the RcsA protein in vivo depends on the Lon protease. Its stability is increased by RcsB. RcsA* is more stable than RcsA.
Sequence similarities	Contains 1 HTH luxR-type DNA-binding domain.

Ontologies

Keywords
Biological process	Capsule biogenesis/degradation Sensory transduction Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Activator
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: GOC transcription, DNA-dependent Inferred from direct assay PubMed 10702265PubMed 12864862. Source: EcoCyc
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	phosphorelay response regulator activity Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 207	207	Colanic acid capsular biosynthesis activation protein A		PRO_0000184178
Regions
	Domain	131 – 196	66	HTH luxR-type
	DNA binding	155 – 174	20	H-T-H motif By similarity
Natural variations
	Natural variant	67	1	R → H in strain: O9:K30:H12.
	Natural variant	70	1	L → H in strain: O9:K30:H12.
Experimental info
	Mutagenesis	145	1	M → V in rcsA*; increased capsule synthesis. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P69405 [UniParc]. Last modified March 1, 2005. Version 1. Checksum: 6DAA22097807AF62	FASTA	207	23,516
	10 20 30 40 50 60 MSTIIMDLCS YTRLGLTGYL LSRGVKKREI NDIETVDDLA IACDSQRPSV VFINEDCFIH 70 80 90 100 110 120 DASNSQRIKL IINQHPNTLF IVFMAIANVH FDEYLLVRKN LLISSKSIKP ESLDDILGDI 130 140 150 160 170 180 LKKETTITSF LNMPTLSLSR TESSMLRMWM AGQGTIQISD QMNIKAKTVS SHKGNIKRKI 190 200 KTHNKQVIYH VVRLTDNVTN GIFVNMR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"RcsA, an unstable positive regulator of capsular polysaccharide synthesis." Stout V., Torres-Cabassa A., Maurizi M.R., Gutnick D., Gottesman S. J. Bacteriol. 173:1738-1747(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-8, MUTAGENESIS OF MET-145.
[2]	"The rcsA gene of Escherichia coli 09:K30:H12 is involved in the expression of the serotype-specific group I K (capsular) antigen." Keenleyside W.J., Jayaratne P., MacLachlan P.R., Whitfield C. J. Bacteriol. 174:8-16(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: O9:K30:H12.
[3]	"A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S. , Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y., Horiuchi T. DNA Res. 3:379-392(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[5]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[6]	"A small RNA acts as an antisilencer of the H-NS-silenced rcsA gene of Escherichia coli." Sledjeski D., Gottesman S. Proc. Natl. Acad. Sci. U.S.A. 92:2003-2007(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-20. Strain: K12.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M58003 Genomic DNA. Translation: AAA82970.1. M81191 Genomic DNA. Translation: AAA24502.1. U00096 Genomic DNA. Translation: AAC75018.1. AP009048 Genomic DNA. Translation: BAA15776.1. U17137 Genomic DNA. Translation: AAC43315.1.
PIR	A37320.
RefSeq	NP_416461.1. NC_000913.2. YP_490205.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P69405.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-48090N.
IntAct	P69405. 29 interactions.
STRING	511145.b1951.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC75018; AAC75018; b1951. BAA15776; BAA15776; BAA15776.
GeneID	12932053. 946467.
KEGG	ecj:Y75_p1921. eco:b1951.
PATRIC	32119231. VBIEscCol129921_2030.
Organism-specific databases
EchoBASE	EB0813.
EcoGene	EG10820. rcsA.
Phylogenomic databases
eggNOG	COG2771.
HOGENOM	HOG000121332.
KO	K07781.
OMA	EDCFIHE.
ProtClustDB	PRK15411.
Enzyme and pathway databases
BioCyc	EcoCyc:PD02233. ECOL316407:JW1935-MONOMER.
Gene expression databases
Genevestigator	P69405.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR016032. Sig_transdc_resp-reg_C-effctor. IPR000792. Tscrpt_reg_LuxR_C. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
Pfam	PF00196. GerE. 1 hit. [Graphical view]
PRINTS	PR00038. HTHLUXR.
SMART	SM00421. HTH_LUXR. 1 hit. [Graphical view]
SUPFAM	SSF46894. Bipartite_resp_reg_C-effector. 1 hit.
PROSITE	PS00622. HTH_LUXR_1. 1 hit. PS50043. HTH_LUXR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RCSA_ECOLI

Accession

Primary (citable) accession number: P69405
Secondary accession number(s): P24210, Q47585

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2005
Last sequence update:	March 1, 2005
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program