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Protein

Ferrous-iron efflux pump FieF

Gene

fieF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Iron-efflux transporter responsible for iron detoxification. Also able to transport Zn2+ in a proton-dependent manner.1 Publication

GO - Molecular functioni

  • cadmium ion transmembrane transporter activity Source: EcoCyc
  • ferrous iron transmembrane transporter activity Source: EcoCyc
  • zinc efflux active transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • cadmium ion transport Source: EcoCyc
  • cellular cadmium ion homeostasis Source: EcoCyc
  • cellular zinc ion homeostasis Source: EcoCyc
  • ferrous iron transport Source: EcoCyc
  • iron ion homeostasis Source: UniProtKB-KW
  • regulation of sequestering of zinc ion Source: GO_Central
  • response to zinc ion Source: GO_Central
  • zinc II ion transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Ion transport, Iron transport, Transport

Keywords - Ligandi

Iron

Enzyme and pathway databases

BioCyciEcoCyc:YIIP-MONOMER.
ECOL316407:JW3886-MONOMER.
MetaCyc:YIIP-MONOMER.

Protein family/group databases

TCDBi2.A.4.7.1. the cation diffusion facilitator (cdf) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Ferrous-iron efflux pump FieF
Gene namesi
Name:fieF
Synonyms:yiiP
Ordered Locus Names:b3915, JW3886
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11873. fieF.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1111CytoplasmicSequence analysisAdd
BLAST
Transmembranei12 – 3423HelicalSequence analysisAdd
BLAST
Topological domaini35 – 373PeriplasmicSequence analysis
Transmembranei38 – 6023HelicalSequence analysisAdd
BLAST
Topological domaini61 – 7919CytoplasmicSequence analysisAdd
BLAST
Transmembranei80 – 10223HelicalSequence analysisAdd
BLAST
Topological domaini103 – 11614PeriplasmicSequence analysisAdd
BLAST
Transmembranei117 – 13923HelicalSequence analysisAdd
BLAST
Topological domaini140 – 15718CytoplasmicSequence analysisAdd
BLAST
Transmembranei158 – 17518HelicalSequence analysisAdd
BLAST
Topological domaini176 – 1794PeriplasmicSequence analysis
Transmembranei180 – 19920HelicalSequence analysisAdd
BLAST
Topological domaini200 – 300101CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 300300Ferrous-iron efflux pump FieFPRO_0000206124Add
BLAST

Proteomic databases

PaxDbiP69380.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi4261076. 3 interactions.
DIPiDIP-48564N.
STRINGi511145.b3915.

Structurei

Secondary structure

1
300
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi12 – 2110Combined sources
Helixi24 – 296Combined sources
Beta strandi33 – 375Combined sources
Turni42 – 443Combined sources
Helixi45 – 6319Combined sources
Helixi78 – 10326Combined sources
Beta strandi105 – 1084Combined sources
Helixi119 – 14123Combined sources
Helixi145 – 15814Combined sources
Beta strandi166 – 1683Combined sources
Helixi180 – 20526Combined sources
Helixi212 – 22413Combined sources
Beta strandi225 – 2273Combined sources
Beta strandi229 – 23911Combined sources
Beta strandi242 – 2509Combined sources
Helixi257 – 27418Combined sources
Beta strandi279 – 2857Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2QFIX-ray3.80A/B1-300[»]
3H90X-ray2.90A/B/C/D8-290[»]
ProteinModelPortaliP69380.
SMRiP69380. Positions 5-290.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69380.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG41076HS. Bacteria.
COG0053. LUCA.
HOGENOMiHOG000069673.
InParanoidiP69380.
KOiK13283.
OMAiFQRWVIR.
OrthoDBiEOG6VQPXP.
PhylomeDBiP69380.

Family and domain databases

Gene3Di1.20.1510.10. 1 hit.
3.30.70.1350. 1 hit.
HAMAPiMF_01425. Cation_efflux_FieF.
InterProiIPR002524. Cation_efflux.
IPR027470. Cation_efflux_CTD.
IPR023783. Cation_efflux_FieF.
IPR027469. Cation_efflux_TMD.
[Graphical view]
PANTHERiPTHR11562. PTHR11562. 1 hit.
PfamiPF01545. Cation_efflux. 1 hit.
PF16916. ZT_dimer. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01297. CDF. 1 hit.

Sequencei

Sequence statusi: Complete.

P69380-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQSYGRLVS RAAIAATAMA SLLLLIKIFA WWYTGSVSIL AALVDSLVDI
60 70 80 90 100
GASLTNLLVV RYSLQPADDN HSFGHGKAES LAALAQSMFI SGSALFLFLT
110 120 130 140 150
GIQHLISPTP MTDPGVGVIV TIVALICTII LVSFQRWVVR RTQSQAVRAD
160 170 180 190 200
MLHYQSDVMM NGAILLALGL SWYGWHRADA LFALGIGIYI LYSALRMGYE
210 220 230 240 250
AVQSLLDRAL PDEERQEIID IVTSWPGVSG AHDLRTRQSG PTRFIQIHLE
260 270 280 290 300
MEDSLPLVQA HMVADQVEQA ILRRFPGSDV IIHQDPCSVV PREGKRSMLS
Length:300
Mass (Da):32,927
Last modified:March 1, 2005 - v1
Checksum:iA9ECC4D6ED9A750F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19201 Genomic DNA. Translation: AAB03047.1.
U00096 Genomic DNA. Translation: AAC76897.1.
AP009048 Genomic DNA. Translation: BAE77395.1.
PIRiS40858.
RefSeqiNP_418350.1. NC_000913.3.
WP_001076742.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76897; AAC76897; b3915.
BAE77395; BAE77395; BAE77395.
GeneIDi948413.
KEGGiecj:JW3886.
eco:b3915.
PATRICi32123341. VBIEscCol129921_4031.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19201 Genomic DNA. Translation: AAB03047.1.
U00096 Genomic DNA. Translation: AAC76897.1.
AP009048 Genomic DNA. Translation: BAE77395.1.
PIRiS40858.
RefSeqiNP_418350.1. NC_000913.3.
WP_001076742.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2QFIX-ray3.80A/B1-300[»]
3H90X-ray2.90A/B/C/D8-290[»]
ProteinModelPortaliP69380.
SMRiP69380. Positions 5-290.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261076. 3 interactions.
DIPiDIP-48564N.
STRINGi511145.b3915.

Protein family/group databases

TCDBi2.A.4.7.1. the cation diffusion facilitator (cdf) family.

Proteomic databases

PaxDbiP69380.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76897; AAC76897; b3915.
BAE77395; BAE77395; BAE77395.
GeneIDi948413.
KEGGiecj:JW3886.
eco:b3915.
PATRICi32123341. VBIEscCol129921_4031.

Organism-specific databases

EchoBASEiEB1819.
EcoGeneiEG11873. fieF.

Phylogenomic databases

eggNOGiENOG41076HS. Bacteria.
COG0053. LUCA.
HOGENOMiHOG000069673.
InParanoidiP69380.
KOiK13283.
OMAiFQRWVIR.
OrthoDBiEOG6VQPXP.
PhylomeDBiP69380.

Enzyme and pathway databases

BioCyciEcoCyc:YIIP-MONOMER.
ECOL316407:JW3886-MONOMER.
MetaCyc:YIIP-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP69380.
PROiP69380.

Family and domain databases

Gene3Di1.20.1510.10. 1 hit.
3.30.70.1350. 1 hit.
HAMAPiMF_01425. Cation_efflux_FieF.
InterProiIPR002524. Cation_efflux.
IPR027470. Cation_efflux_CTD.
IPR023783. Cation_efflux_FieF.
IPR027469. Cation_efflux_TMD.
[Graphical view]
PANTHERiPTHR11562. PTHR11562. 1 hit.
PfamiPF01545. Cation_efflux. 1 hit.
PF16916. ZT_dimer. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01297. CDF. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes."
    Plunkett G. III, Burland V., Daniels D.L., Blattner F.R.
    Nucleic Acids Res. 21:3391-3398(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Oligomeric state of the Escherichia coli metal transporter YiiP."
    Wei Y., Li H., Fu D.
    J. Biol. Chem. 279:39251-39259(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT.
  5. "FieF (YiiP) from Escherichia coli mediates decreased cellular accumulation of iron and relieves iron stress."
    Grass G., Otto M., Fricke B., Haney C.J., Rensing C., Nies D.H., Munkelt D.
    Arch. Microbiol. 183:9-18(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiFIEF_ECOLI
AccessioniPrimary (citable) accession number: P69380
Secondary accession number(s): P32159, Q2M8L1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: July 6, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.