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Protein

Ferrous-iron efflux pump FieF

Gene

fieF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Iron-efflux transporter responsible for iron detoxification. Also able to transport Zn2+ in a proton-dependent manner.1 Publication

GO - Molecular functioni

  • cadmium ion transmembrane transporter activity Source: EcoCyc
  • ferrous iron transmembrane transporter activity Source: EcoCyc
  • zinc efflux active transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • cadmium ion transport Source: EcoCyc
  • cellular cadmium ion homeostasis Source: EcoCyc
  • cellular zinc ion homeostasis Source: EcoCyc
  • ferrous iron transport Source: EcoCyc
  • iron ion homeostasis Source: UniProtKB-KW
  • regulation of sequestering of zinc ion Source: GO_Central
  • response to zinc ion Source: GO_Central
  • zinc II ion transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Ion transport, Iron transport, Transport

Keywords - Ligandi

Iron

Enzyme and pathway databases

BioCyciEcoCyc:YIIP-MONOMER.
ECOL316407:JW3886-MONOMER.
MetaCyc:YIIP-MONOMER.

Protein family/group databases

TCDBi2.A.4.7.1. the cation diffusion facilitator (cdf) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Ferrous-iron efflux pump FieF
Gene namesi
Name:fieF
Synonyms:yiiP
Ordered Locus Names:b3915, JW3886
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11873. fieF.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 11CytoplasmicSequence analysisAdd BLAST11
Transmembranei12 – 34HelicalSequence analysisAdd BLAST23
Topological domaini35 – 37PeriplasmicSequence analysis3
Transmembranei38 – 60HelicalSequence analysisAdd BLAST23
Topological domaini61 – 79CytoplasmicSequence analysisAdd BLAST19
Transmembranei80 – 102HelicalSequence analysisAdd BLAST23
Topological domaini103 – 116PeriplasmicSequence analysisAdd BLAST14
Transmembranei117 – 139HelicalSequence analysisAdd BLAST23
Topological domaini140 – 157CytoplasmicSequence analysisAdd BLAST18
Transmembranei158 – 175HelicalSequence analysisAdd BLAST18
Topological domaini176 – 179PeriplasmicSequence analysis4
Transmembranei180 – 199HelicalSequence analysisAdd BLAST20
Topological domaini200 – 300CytoplasmicSequence analysisAdd BLAST101

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002061241 – 300Ferrous-iron efflux pump FieFAdd BLAST300

Proteomic databases

PaxDbiP69380.
PRIDEiP69380.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi4261076. 3 interactors.
DIPiDIP-48564N.
STRINGi511145.b3915.

Structurei

Secondary structure

1300
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 21Combined sources10
Helixi24 – 29Combined sources6
Beta strandi33 – 37Combined sources5
Turni42 – 44Combined sources3
Helixi45 – 63Combined sources19
Helixi78 – 103Combined sources26
Beta strandi105 – 108Combined sources4
Helixi119 – 141Combined sources23
Helixi145 – 158Combined sources14
Beta strandi166 – 168Combined sources3
Helixi180 – 205Combined sources26
Helixi212 – 224Combined sources13
Beta strandi225 – 227Combined sources3
Beta strandi229 – 239Combined sources11
Beta strandi242 – 250Combined sources9
Helixi257 – 274Combined sources18
Beta strandi279 – 285Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QFIX-ray3.80A/B1-300[»]
3H90X-ray2.90A/B/C/D8-290[»]
ProteinModelPortaliP69380.
SMRiP69380.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69380.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG41076HS. Bacteria.
COG0053. LUCA.
HOGENOMiHOG000069673.
InParanoidiP69380.
KOiK13283.
OMAiFQRWVIR.
PhylomeDBiP69380.

Family and domain databases

Gene3Di1.20.1510.10. 1 hit.
3.30.70.1350. 1 hit.
HAMAPiMF_01425. Cation_efflux_FieF. 1 hit.
InterProiIPR002524. Cation_efflux.
IPR027470. Cation_efflux_CTD.
IPR023783. Cation_efflux_FieF.
IPR027469. Cation_efflux_TMD.
[Graphical view]
PANTHERiPTHR11562. PTHR11562. 1 hit.
PfamiPF01545. Cation_efflux. 1 hit.
PF16916. ZT_dimer. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01297. CDF. 1 hit.

Sequencei

Sequence statusi: Complete.

P69380-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNQSYGRLVS RAAIAATAMA SLLLLIKIFA WWYTGSVSIL AALVDSLVDI
60 70 80 90 100
GASLTNLLVV RYSLQPADDN HSFGHGKAES LAALAQSMFI SGSALFLFLT
110 120 130 140 150
GIQHLISPTP MTDPGVGVIV TIVALICTII LVSFQRWVVR RTQSQAVRAD
160 170 180 190 200
MLHYQSDVMM NGAILLALGL SWYGWHRADA LFALGIGIYI LYSALRMGYE
210 220 230 240 250
AVQSLLDRAL PDEERQEIID IVTSWPGVSG AHDLRTRQSG PTRFIQIHLE
260 270 280 290 300
MEDSLPLVQA HMVADQVEQA ILRRFPGSDV IIHQDPCSVV PREGKRSMLS
Length:300
Mass (Da):32,927
Last modified:March 1, 2005 - v1
Checksum:iA9ECC4D6ED9A750F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19201 Genomic DNA. Translation: AAB03047.1.
U00096 Genomic DNA. Translation: AAC76897.1.
AP009048 Genomic DNA. Translation: BAE77395.1.
PIRiS40858.
RefSeqiNP_418350.1. NC_000913.3.
WP_001076742.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76897; AAC76897; b3915.
BAE77395; BAE77395; BAE77395.
GeneIDi948413.
KEGGiecj:JW3886.
eco:b3915.
PATRICi32123341. VBIEscCol129921_4031.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L19201 Genomic DNA. Translation: AAB03047.1.
U00096 Genomic DNA. Translation: AAC76897.1.
AP009048 Genomic DNA. Translation: BAE77395.1.
PIRiS40858.
RefSeqiNP_418350.1. NC_000913.3.
WP_001076742.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2QFIX-ray3.80A/B1-300[»]
3H90X-ray2.90A/B/C/D8-290[»]
ProteinModelPortaliP69380.
SMRiP69380.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4261076. 3 interactors.
DIPiDIP-48564N.
STRINGi511145.b3915.

Protein family/group databases

TCDBi2.A.4.7.1. the cation diffusion facilitator (cdf) family.

Proteomic databases

PaxDbiP69380.
PRIDEiP69380.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76897; AAC76897; b3915.
BAE77395; BAE77395; BAE77395.
GeneIDi948413.
KEGGiecj:JW3886.
eco:b3915.
PATRICi32123341. VBIEscCol129921_4031.

Organism-specific databases

EchoBASEiEB1819.
EcoGeneiEG11873. fieF.

Phylogenomic databases

eggNOGiENOG41076HS. Bacteria.
COG0053. LUCA.
HOGENOMiHOG000069673.
InParanoidiP69380.
KOiK13283.
OMAiFQRWVIR.
PhylomeDBiP69380.

Enzyme and pathway databases

BioCyciEcoCyc:YIIP-MONOMER.
ECOL316407:JW3886-MONOMER.
MetaCyc:YIIP-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP69380.
PROiP69380.

Family and domain databases

Gene3Di1.20.1510.10. 1 hit.
3.30.70.1350. 1 hit.
HAMAPiMF_01425. Cation_efflux_FieF. 1 hit.
InterProiIPR002524. Cation_efflux.
IPR027470. Cation_efflux_CTD.
IPR023783. Cation_efflux_FieF.
IPR027469. Cation_efflux_TMD.
[Graphical view]
PANTHERiPTHR11562. PTHR11562. 1 hit.
PfamiPF01545. Cation_efflux. 1 hit.
PF16916. ZT_dimer. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01297. CDF. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiFIEF_ECOLI
AccessioniPrimary (citable) accession number: P69380
Secondary accession number(s): P32159, Q2M8L1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: November 2, 2016
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.