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Protein

Multidrug resistance protein MdtH

Gene

mdtH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Confers resistance to norfloxacin and enoxacin.1 Publication

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Antibiotic resistance, Transport

Enzyme and pathway databases

BioCyciEcoCyc:B1065-MONOMER.
ECOL316407:JW1052-MONOMER.

Protein family/group databases

TCDBi2.A.1.2.21. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Multidrug resistance protein MdtH
Gene namesi
Name:mdtH
Synonyms:yceL
Ordered Locus Names:b1065, JW1052
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13878. mdtH.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212CytoplasmicSequence analysisAdd
BLAST
Transmembranei13 – 3321HelicalSequence analysisAdd
BLAST
Topological domaini34 – 9865PeriplasmicSequence analysisAdd
BLAST
Transmembranei99 – 11618HelicalSequence analysisAdd
BLAST
Topological domaini117 – 13822CytoplasmicSequence analysisAdd
BLAST
Transmembranei139 – 15921HelicalSequence analysisAdd
BLAST
Topological domaini160 – 1645PeriplasmicSequence analysis
Transmembranei165 – 18521HelicalSequence analysisAdd
BLAST
Topological domaini186 – 21328CytoplasmicSequence analysisAdd
BLAST
Transmembranei214 – 23421HelicalSequence analysisAdd
BLAST
Topological domaini235 – 2439PeriplasmicSequence analysis
Transmembranei244 – 26421HelicalSequence analysisAdd
BLAST
Topological domaini265 – 27612CytoplasmicSequence analysisAdd
BLAST
Transmembranei277 – 29721HelicalSequence analysisAdd
BLAST
Topological domaini298 – 2992PeriplasmicSequence analysis
Transmembranei300 – 32021HelicalSequence analysisAdd
BLAST
Topological domaini321 – 33919CytoplasmicSequence analysisAdd
BLAST
Transmembranei340 – 36021HelicalSequence analysisAdd
BLAST
Topological domaini361 – 3677PeriplasmicSequence analysis
Transmembranei368 – 38821HelicalSequence analysisAdd
BLAST
Topological domaini389 – 40214CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 402402Multidrug resistance protein MdtHPRO_0000173342Add
BLAST

Proteomic databases

PaxDbiP69367.
PRIDEiP69367.

Expressioni

Inductioni

Induced at least fivefold by indole in a dose-dependent manner.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi4260075. 295 interactions.
IntActiP69367. 1 interaction.
STRINGi511145.b1065.

Structurei

3D structure databases

ProteinModelPortaliP69367.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EV4. Bacteria.
ENOG410XSK2. LUCA.
HOGENOMiHOG000266157.
InParanoidiP69367.
KOiK08162.
OMAiTHAPSMM.
OrthoDBiEOG67Q97V.
PhylomeDBiP69367.

Family and domain databases

HAMAPiMF_01529. MFS_MdtH.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR022855. Multidrug-R_MdtH.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P69367-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRVSQARNL GKYFLLIDNM LVVLGFFVVF PLISIRFVDQ MGWAAVMVGI
60 70 80 90 100
ALGLRQFIQQ GLGIFGGAIA DRFGAKPMIV TGMLMRAAGF ATMGIAHEPW
110 120 130 140 150
LLWFSCLLSG LGGTLFDPPR SALVVKLIRP QQRGRFFSLL MMQDSAGAVI
160 170 180 190 200
GALLGSWLLQ YDFRLVCATG AVLFVLCAAF NAWLLPAWKL STVRTPVREG
210 220 230 240 250
MTRVMRDKRF VTYVLTLAGY YMLAVQVMLM LPIMVNDVAG APSAVKWMYA
260 270 280 290 300
IEACLSLTLL YPIARWSEKH FRLEHRLMAG LLIMSLSMMP VGMVSGLQQL
310 320 330 340 350
FTLICLFYIG SIIAEPARET LSASLADARA RGSYMGFSRL GLAIGGAIGY
360 370 380 390 400
IGGGWLFDLG KSAHQPELPW MMLGIIGIFT FLALGWQFSQ KRAARRLLER

DA
Length:402
Mass (Da):44,363
Last modified:March 1, 2005 - v1
Checksum:i7220F064CD012BB4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti322 – 3232SA → RS in CAA63057 (PubMed:9111025).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92076 Genomic DNA. Translation: CAA63057.1.
U00096 Genomic DNA. Translation: AAC74149.2.
AP009048 Genomic DNA. Translation: BAA35873.2.
PIRiF64849.
RefSeqiNP_415583.4. NC_000913.3.
WP_000092206.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74149; AAC74149; b1065.
BAA35873; BAA35873; BAA35873.
GeneIDi946920.
KEGGiecj:JW1052.
eco:b1065.
PATRICi32117369. VBIEscCol129921_1107.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92076 Genomic DNA. Translation: CAA63057.1.
U00096 Genomic DNA. Translation: AAC74149.2.
AP009048 Genomic DNA. Translation: BAA35873.2.
PIRiF64849.
RefSeqiNP_415583.4. NC_000913.3.
WP_000092206.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP69367.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260075. 295 interactions.
IntActiP69367. 1 interaction.
STRINGi511145.b1065.

Protein family/group databases

TCDBi2.A.1.2.21. the major facilitator superfamily (mfs).

Proteomic databases

PaxDbiP69367.
PRIDEiP69367.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74149; AAC74149; b1065.
BAA35873; BAA35873; BAA35873.
GeneIDi946920.
KEGGiecj:JW1052.
eco:b1065.
PATRICi32117369. VBIEscCol129921_1107.

Organism-specific databases

EchoBASEiEB3637.
EcoGeneiEG13878. mdtH.

Phylogenomic databases

eggNOGiENOG4105EV4. Bacteria.
ENOG410XSK2. LUCA.
HOGENOMiHOG000266157.
InParanoidiP69367.
KOiK08162.
OMAiTHAPSMM.
OrthoDBiEOG67Q97V.
PhylomeDBiP69367.

Enzyme and pathway databases

BioCyciEcoCyc:B1065-MONOMER.
ECOL316407:JW1052-MONOMER.

Miscellaneous databases

PROiP69367.

Family and domain databases

HAMAPiMF_01529. MFS_MdtH.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR022855. Multidrug-R_MdtH.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, overexpression, and characterization of glutaredoxin 2, an atypical glutaredoxin from Escherichia coli."
    Vlamis-Gardikas A., Aaslund F., Spyrou G., Bergman T., Holmgren A.
    J. Biol. Chem. 272:11236-11243(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: K12.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Analysis of a complete library of putative drug transporter genes in Escherichia coli."
    Nishino K., Yamaguchi A.
    J. Bacteriol. 183:5803-5812(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Indole induces the expression of multidrug exporter genes in Escherichia coli."
    Hirakawa H., Inazumi Y., Masaki T., Hirata T., Yamaguchi A.
    Mol. Microbiol. 55:1113-1126(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
    Strain: K12.
  7. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiMDTH_ECOLI
AccessioniPrimary (citable) accession number: P69367
Secondary accession number(s): P75929, P77042, Q9R7P2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: March 1, 2005
Last modified: April 13, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.