Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

G-protein coupled receptor homolog US28

Gene

US28

Organism
Human cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Receptor for a C-C type chemokine. Binds to a number of different CC-chemokines including CCL5/RANTES, CCL2/MCP-1, CCL3/MIP-1-alpha as well as CX3CL1/Fractalkine. Transduces signals resulting in the activation of MAP kinase signaling pathways and augmentation of intracellular calcium ion levels, leading to alterations in chemotactic behavior of vascular smooth muscle cells and macrophages. The US28 receptor also exhibits high levels of agonist-independent signaling activity and agonist-independent endocytosis. Interacts with endogenous Gaq/11 subunits and thereby constitutively activates phospholipase C.2 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionG-protein coupled receptor, Receptor, Transducer
Biological processHost-virus interaction, Inhibition of host chemokines by virus, Viral immunoevasion

Names & Taxonomyi

Protein namesi
Recommended name:
G-protein coupled receptor homolog US28
Alternative name(s):
HHRF3
Gene namesi
Name:US28
OrganismiHuman cytomegalovirus (strain AD169) (HHV-5) (Human herpesvirus 5)
Taxonomic identifieri10360 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeBetaherpesvirinaeCytomegalovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000008991 Componenti: Genome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 37ExtracellularSequence analysisAdd BLAST37
Transmembranei38 – 58Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini59 – 69CytoplasmicSequence analysisAdd BLAST11
Transmembranei70 – 90Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini91 – 101ExtracellularSequence analysisAdd BLAST11
Transmembranei102 – 122Helical; Name=3Sequence analysisAdd BLAST21
Topological domaini123 – 145CytoplasmicSequence analysisAdd BLAST23
Transmembranei146 – 166Helical; Name=4Sequence analysisAdd BLAST21
Topological domaini167 – 183ExtracellularSequence analysisAdd BLAST17
Transmembranei184 – 204Helical; Name=5Sequence analysisAdd BLAST21
Topological domaini205 – 228CytoplasmicSequence analysisAdd BLAST24
Transmembranei229 – 249Helical; Name=6Sequence analysisAdd BLAST21
Topological domaini250 – 273ExtracellularSequence analysisAdd BLAST24
Transmembranei274 – 294Helical; Name=7Sequence analysisAdd BLAST21
Topological domaini295 – 354CytoplasmicSequence analysisAdd BLAST60

GO - Cellular componenti

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4259

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000702461 – 354G-protein coupled receptor homolog US28Add BLAST354

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi30N-linked (GlcNAc...) asparagine; by hostSequence analysis1

Post-translational modificationi

Phosphorylated. High phosphorylation occurs concomitantly with receptor endocytosis and correlate with low receptor presence at the plasma membrane.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiP69332

PTM databases

iPTMnetiP69332

Interactioni

Subunit structurei

Interacts with host GPRASP1; this interaction targets US28 to lysosomes for degradation.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CX3CL1P784234EBI-16147206,EBI-15188013From Homo sapiens.

Protein-protein interaction databases

DIPiDIP-61489N
IntActiP69332 1 interactor.

Chemistry databases

BindingDBiP69332

Structurei

Secondary structure

1354
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi26 – 59Combined sources34
Helixi67 – 83Combined sources17
Helixi86 – 94Combined sources9
Helixi104 – 133Combined sources30
Helixi141 – 157Combined sources17
Helixi160 – 163Combined sources4
Beta strandi166 – 169Combined sources4
Beta strandi172 – 175Combined sources4
Beta strandi177 – 180Combined sources4
Helixi184 – 196Combined sources13
Helixi198 – 215Combined sources18
Helixi223 – 255Combined sources33
Helixi263 – 280Combined sources18
Helixi283 – 290Combined sources8
Turni291 – 294Combined sources4
Helixi296 – 308Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4XT1X-ray2.89A1-354[»]
4XT3X-ray3.80A1-354[»]
ProteinModelPortaliP69332
SMRiP69332
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi2 – 7Poly-Thr6

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

OrthoDBiVOG090000NY

Family and domain databases

InterProiView protein in InterPro
IPR000355 Chemokine_rcpt
IPR000276 GPCR_Rhodpsn
IPR017452 GPCR_Rhodpsn_7TM
PfamiView protein in Pfam
PF00001 7tm_1, 1 hit
PRINTSiPR00657 CCCHEMOKINER
PR00237 GPCRRHODOPSN
PROSITEiView protein in PROSITE
PS00237 G_PROTEIN_RECEP_F1_1, 1 hit
PS50262 G_PROTEIN_RECEP_F1_2, 1 hit

Sequencei

Sequence statusi: Complete.

P69332-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTPTTTTAEL TTEFDYDEDA TPCVFTDVLN QSKPVTLFLY GVVFLFGSIG
60 70 80 90 100
NFLVIFTITW RRRIQCSGDV YFINLAAADL LFVCTLPLWM QYLLDHNSLA
110 120 130 140 150
SVPCTLLTAC FYVAMFASLC FITEIALDRY YAIVYMRYRP VKQACLFSIF
160 170 180 190 200
WWIFAVIIAI PHFMVVTKKD NQCMTDYDYL EVSYPIILNV ELMLGAFVIP
210 220 230 240 250
LSVISYCYYR ISRIVAVSQS RHKGRIVRVL IAVVLVFIIF WLPYHLTLFV
260 270 280 290 300
DTLKLLKWIS SSCEFERSLK RALILTESLA FCHCCLNPLL YVFVGTKFRQ
310 320 330 340 350
ELHCLLAEFR QRLFSRDVSW YHSMSFSRRS SPSRRETSSD TLSDEVCRVS

QIIP
Length:354
Mass (Da):41,064
Last modified:February 15, 2005 - v1
Checksum:i295A2F7C54DF7AAD
GO

Sequence cautioni

The sequence CAA28338 differs from that shown. Reason: Frameshift at position 299.Curated
The sequence CAA35260 differs from that shown. Reason: Frameshift at position 299.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti18 – 19ED → DE in strain: Isolate clinical VHL/E. 2
Natural varianti25F → L in strain: Isolate clinical VHL/E. 1
Natural varianti267R → K in strain: Isolate clinical VHL/E. 1
Natural varianti346V → A in strain: Isolate clinical VHL/E. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04650 Genomic DNA Translation: CAA28338.1 Frameshift.
X17403 Genomic DNA Translation: CAA35260.1 Frameshift.
L20501 Genomic DNA Translation: AAA98741.1
PIRiC27216 QQBED3

Similar proteinsi

Entry informationi

Entry nameiUS28_HCMVA
AccessioniPrimary (citable) accession number: P69332
Secondary accession number(s): P09704, P32952, Q7M6H3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: February 15, 2005
Last modified: April 25, 2018
This is version 75 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome