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P69249

- RBS_TOBAC

UniProt

P69249 - RBS_TOBAC

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Protein

Ribulose bisphosphate carboxylase small chain, chloroplastic

Gene

RBCS

Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site.

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.

GO - Molecular functioni

  1. monooxygenase activity Source: UniProtKB-KW
  2. ribulose-bisphosphate carboxylase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbon fixation Source: UniProtKB-KW
  2. photorespiration Source: UniProtKB-KW
  3. photosynthesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Lyase, Monooxygenase, Oxidoreductase

Keywords - Biological processi

Carbon dioxide fixation, Photorespiration, Photosynthesis

Enzyme and pathway databases

SABIO-RKP69249.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylase small chain, chloroplastic (EC:4.1.1.39)
Short name:
RuBisCO small subunit
Alternative name(s):
TSSU3-8
Gene namesi
Name:RBCS
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5757Chloroplast1 PublicationAdd
BLAST
Chaini58 – 180123Ribulose bisphosphate carboxylase small chain, chloroplasticPRO_0000031560Add
BLAST

Interactioni

Subunit structurei

8 large chains + 8 small chains.

Binary interactionsi

WithEntry#Exp.IntActNotes
TOC159O812835EBI-1766821,EBI-639063From a different organism.
TOC34Q389066EBI-1766821,EBI-1766808From a different organism.

Protein-protein interaction databases

DIPiDIP-40635N.
IntActiP69249. 3 interactions.
MINTiMINT-1176585.

Structurei

Secondary structure

1
180
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi63 – 653Combined sources
Turni71 – 744Combined sources
Helixi80 – 9213Combined sources
Beta strandi96 – 1049Combined sources
Beta strandi125 – 1284Combined sources
Helixi137 – 15014Combined sources
Beta strandi154 – 1629Combined sources
Turni163 – 1664Combined sources
Beta strandi167 – 1759Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1EJ7X-ray2.45S58-180[»]
1RLCX-ray2.70S58-180[»]
1RLDX-ray2.50S/T58-180[»]
3RUBX-ray2.00S58-180[»]
4RUBX-ray2.70S/T/U/V58-180[»]
ProteinModelPortaliP69249.
SMRiP69249. Positions 58-180.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69249.

Family & Domainsi

Sequence similaritiesi

Belongs to the RuBisCO small chain family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.30.190.10. 1 hit.
InterProiIPR024681. RuBisCO_sc.
IPR000894. RuBisCO_sc_dom.
IPR024680. RuBisCO_ssu_N.
[Graphical view]
PfamiPF12338. RbcS. 1 hit.
PF00101. RuBisCO_small. 1 hit.
[Graphical view]
PRINTSiPR00152. RUBISCOSMALL.
SUPFAMiSSF55239. SSF55239. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P69249-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASSVLSSAA VATRSNVAQA NMVAPFTGLK SAASFPVSRK QNLDITSIAS
60 70 80 90 100
NGGRVQCMQV WPPINKKKYE TLSYLPDLSQ EQLLSEVEYL LKNGWVPCLE
110 120 130 140 150
FETEHGFVYR ENNKSPGYYD GRYWTMWKLP MFGCTDATQV LAEVEEAKKA
160 170 180
YPQAWIRIIG FDNVRQVQCI SFIAYKPEGY
Length:180
Mass (Da):20,311
Last modified:July 21, 1986 - v1
Checksum:i26CF3F48EF0860AD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti87 – 882VE → PD AA sequence 1 PublicationCurated
Sequence conflicti93 – 931N → D AA sequence 1 PublicationCurated
Sequence conflicti153 – 1531Q → E AA sequence 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02353 Genomic DNA. Translation: CAA26208.1.
M32419 Genomic DNA. Translation: AAA34116.1.
PIRiA22934. RKNTSP.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02353 Genomic DNA. Translation: CAA26208.1 .
M32419 Genomic DNA. Translation: AAA34116.1 .
PIRi A22934. RKNTSP.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1EJ7 X-ray 2.45 S 58-180 [» ]
1RLC X-ray 2.70 S 58-180 [» ]
1RLD X-ray 2.50 S/T 58-180 [» ]
3RUB X-ray 2.00 S 58-180 [» ]
4RUB X-ray 2.70 S/T/U/V 58-180 [» ]
ProteinModelPortali P69249.
SMRi P69249. Positions 58-180.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-40635N.
IntActi P69249. 3 interactions.
MINTi MINT-1176585.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

SABIO-RK P69249.

Miscellaneous databases

EvolutionaryTracei P69249.

Family and domain databases

Gene3Di 3.30.190.10. 1 hit.
InterProi IPR024681. RuBisCO_sc.
IPR000894. RuBisCO_sc_dom.
IPR024680. RuBisCO_ssu_N.
[Graphical view ]
Pfami PF12338. RbcS. 1 hit.
PF00101. RuBisCO_small. 1 hit.
[Graphical view ]
PRINTSi PR00152. RUBISCOSMALL.
SUPFAMi SSF55239. SSF55239. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Sequence of a genomic DNA clone for the small subunit of ribulose bis-phosphate carboxylase-oxygenase from tobacco."
    Mazur B.J., Chui C.-F.
    Nucleic Acids Res. 13:2373-2386(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Amino acid sequence of the small subunit of D-ribulose bisphosphate carboxylase/oxygenase from Nicotiana tabacum."
    Mueller K.-D., Salnikow J., Vater J.
    Biochim. Biophys. Acta 742:78-83(1983)
    Cited for: PROTEIN SEQUENCE OF 58-180.
  3. "Isolation of tobacco SSU genes: characterization of a transcriptionally active pseudogene."
    O'Neal J.K., Pokalsky A.R., Kiehne K.L., Shewmaker C.K.
    Nucleic Acids Res. 15:8661-8677(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-101.
  4. "Crystal structure of the unactivated form of ribulose-1,5-bisphosphate carboxylase/oxygenase from tobacco refined at 2.0-A resolution."
    Curmi P.M.G., Cascio D., Sweet R.M., Eisenberg D., Schreuder H.
    J. Biol. Chem. 267:16980-16989(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).

Entry informationi

Entry nameiRBS_TOBAC
AccessioniPrimary (citable) accession number: P69249
Secondary accession number(s): P00866, P00867, P26666
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 26, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3