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P69249 (RBS_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Ribulose bisphosphate carboxylase small chain, chloroplastic
Short name=RuBisCO small subunit
EC=4.1.1.39
Alternative name(s):
TSSU3-8
Gene names
Name:RBCS
OrganismNicotiana tabacum (Common tobacco)
Taxonomic identifier4097 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length180 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site.

Catalytic activity

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.

3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.

Subunit structure

8 large chains + 8 small chains.

Subcellular location

Plastid › chloroplast.

Sequence similarities

Belongs to the RuBisCO small chain family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

TOC159O812835EBI-1766821,EBI-639063From a different organism.
TOC34Q389066EBI-1766821,EBI-1766808From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5757Chloroplast Ref.2
Chain58 – 180123Ribulose bisphosphate carboxylase small chain, chloroplastic
PRO_0000031560

Experimental info

Sequence conflict87 – 882VE → PD AA sequence Ref.2
Sequence conflict931N → D AA sequence Ref.2
Sequence conflict1531Q → E AA sequence Ref.2

Secondary structure

1 ................. 180
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P69249 [UniParc].

Last modified July 21, 1986. Version 1.
Checksum: 26CF3F48EF0860AD

FASTA18020,311
        10         20         30         40         50         60 
MASSVLSSAA VATRSNVAQA NMVAPFTGLK SAASFPVSRK QNLDITSIAS NGGRVQCMQV 

        70         80         90        100        110        120 
WPPINKKKYE TLSYLPDLSQ EQLLSEVEYL LKNGWVPCLE FETEHGFVYR ENNKSPGYYD 

       130        140        150        160        170        180 
GRYWTMWKLP MFGCTDATQV LAEVEEAKKA YPQAWIRIIG FDNVRQVQCI SFIAYKPEGY

« Hide

References

[1]"Sequence of a genomic DNA clone for the small subunit of ribulose bis-phosphate carboxylase-oxygenase from tobacco."
Mazur B.J., Chui C.-F.
Nucleic Acids Res. 13:2373-2386(1985) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Amino acid sequence of the small subunit of D-ribulose bisphosphate carboxylase/oxygenase from Nicotiana tabacum."
Mueller K.-D., Salnikow J., Vater J.
Biochim. Biophys. Acta 742:78-83(1983)
Cited for: PROTEIN SEQUENCE OF 58-180.
[3]"Isolation of tobacco SSU genes: characterization of a transcriptionally active pseudogene."
O'Neal J.K., Pokalsky A.R., Kiehne K.L., Shewmaker C.K.
Nucleic Acids Res. 15:8661-8677(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-101.
[4]"Crystal structure of the unactivated form of ribulose-1,5-bisphosphate carboxylase/oxygenase from tobacco refined at 2.0-A resolution."
Curmi P.M.G., Cascio D., Sweet R.M., Eisenberg D., Schreuder H.
J. Biol. Chem. 267:16980-16989(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X02353 Genomic DNA. Translation: CAA26208.1.
M32419 Genomic DNA. Translation: AAA34116.1.
PIRRKNTSP. A22934.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1EJ7X-ray2.45S58-180[»]
1RLCX-ray2.70S58-180[»]
1RLDX-ray2.50S/T58-180[»]
3RUBX-ray2.00S58-180[»]
4RUBX-ray2.70S/T/U/V58-180[»]
ProteinModelPortalP69249.
SMRP69249. Positions 58-180.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-40635N.
IntActP69249. 3 interactions.
MINTMINT-1176585.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKP69249.

Family and domain databases

Gene3D3.30.190.10. 1 hit.
InterProIPR024681. RuBisCO_sc.
IPR000894. RuBisCO_sc_dom.
IPR024680. RuBisCO_ssu_N.
[Graphical view]
PfamPF12338. RbcS. 1 hit.
PF00101. RuBisCO_small. 1 hit.
[Graphical view]
PRINTSPR00152. RUBISCOSMALL.
SUPFAMSSF55239. RuBisCO_small. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP69249.

Entry information

Entry nameRBS_TOBAC
AccessionPrimary (citable) accession number: P69249
Secondary accession number(s): P00866, P00867, P26666
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: April 3, 2013
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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