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Protein

Ribulose bisphosphate carboxylase small chain, chloroplastic

Gene

RBCS

Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate (PubMed:3681999). Both reactions occur simultaneously and in competition at the same active site.Curated1 Publication

Catalytic activityi

2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O.1 Publication
3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase, Monooxygenase, Oxidoreductase

Keywords - Biological processi

Carbon dioxide fixation, Photorespiration, Photosynthesis

Enzyme and pathway databases

BRENDAi4.1.1.39. 3645.
SABIO-RKP69249.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribulose bisphosphate carboxylase small chain, chloroplastic (EC:4.1.1.391 Publication)
Short name:
RuBisCO small subunit
Alternative name(s):
TSSU3-8
Gene namesi
Name:RBCS
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 57Chloroplast1 PublicationAdd BLAST57
ChainiPRO_000003156058 – 180Ribulose bisphosphate carboxylase small chain, chloroplasticAdd BLAST123

Proteomic databases

PRIDEiP69249.

Interactioni

Subunit structurei

8 large chains + 8 small chains.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TOC159O812835EBI-1766821,EBI-639063From a different organism.
TOC34Q389066EBI-1766821,EBI-1766808From a different organism.

Protein-protein interaction databases

DIPiDIP-40635N.
IntActiP69249. 3 interactors.
MINTiMINT-1176585.

Structurei

Secondary structure

1180
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi63 – 65Combined sources3
Turni71 – 74Combined sources4
Helixi80 – 92Combined sources13
Beta strandi96 – 104Combined sources9
Beta strandi125 – 128Combined sources4
Helixi137 – 150Combined sources14
Beta strandi154 – 162Combined sources9
Turni163 – 166Combined sources4
Beta strandi167 – 175Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EJ7X-ray2.45S58-180[»]
1RLCX-ray2.70S58-180[»]
1RLDX-ray2.50S/T58-180[»]
3RUBX-ray2.00S58-180[»]
4RUBX-ray2.70S/T/U/V58-180[»]
ProteinModelPortaliP69249.
SMRiP69249.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69249.

Family & Domainsi

Sequence similaritiesi

Belongs to the RuBisCO small chain family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.30.190.10. 1 hit.
InterProiIPR024681. RuBisCO_sc.
IPR000894. RuBisCO_sc_dom.
IPR024680. RuBisCO_ssu_N.
[Graphical view]
PfamiPF12338. RbcS. 1 hit.
PF00101. RuBisCO_small. 1 hit.
[Graphical view]
PRINTSiPR00152. RUBISCOSMALL.
SMARTiSM00961. RuBisCO_small. 1 hit.
[Graphical view]
SUPFAMiSSF55239. SSF55239. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P69249-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASSVLSSAA VATRSNVAQA NMVAPFTGLK SAASFPVSRK QNLDITSIAS
60 70 80 90 100
NGGRVQCMQV WPPINKKKYE TLSYLPDLSQ EQLLSEVEYL LKNGWVPCLE
110 120 130 140 150
FETEHGFVYR ENNKSPGYYD GRYWTMWKLP MFGCTDATQV LAEVEEAKKA
160 170 180
YPQAWIRIIG FDNVRQVQCI SFIAYKPEGY
Length:180
Mass (Da):20,311
Last modified:July 21, 1986 - v1
Checksum:i26CF3F48EF0860AD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti87 – 88VE → PD AA sequence (Ref. 2) Curated2
Sequence conflicti93N → D AA sequence (Ref. 2) Curated1
Sequence conflicti153Q → E AA sequence (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02353 Genomic DNA. Translation: CAA26208.1.
M32419 Genomic DNA. Translation: AAA34116.1.
PIRiA22934. RKNTSP.
RefSeqiXP_016440853.1. XM_016585367.1.
XP_016481946.1. XM_016626460.1.

Genome annotation databases

GeneIDi107766567.
107802883.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X02353 Genomic DNA. Translation: CAA26208.1.
M32419 Genomic DNA. Translation: AAA34116.1.
PIRiA22934. RKNTSP.
RefSeqiXP_016440853.1. XM_016585367.1.
XP_016481946.1. XM_016626460.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EJ7X-ray2.45S58-180[»]
1RLCX-ray2.70S58-180[»]
1RLDX-ray2.50S/T58-180[»]
3RUBX-ray2.00S58-180[»]
4RUBX-ray2.70S/T/U/V58-180[»]
ProteinModelPortaliP69249.
SMRiP69249.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-40635N.
IntActiP69249. 3 interactors.
MINTiMINT-1176585.

Proteomic databases

PRIDEiP69249.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi107766567.
107802883.

Enzyme and pathway databases

BRENDAi4.1.1.39. 3645.
SABIO-RKP69249.

Miscellaneous databases

EvolutionaryTraceiP69249.

Family and domain databases

Gene3Di3.30.190.10. 1 hit.
InterProiIPR024681. RuBisCO_sc.
IPR000894. RuBisCO_sc_dom.
IPR024680. RuBisCO_ssu_N.
[Graphical view]
PfamiPF12338. RbcS. 1 hit.
PF00101. RuBisCO_small. 1 hit.
[Graphical view]
PRINTSiPR00152. RUBISCOSMALL.
SMARTiSM00961. RuBisCO_small. 1 hit.
[Graphical view]
SUPFAMiSSF55239. SSF55239. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRBS_TOBAC
AccessioniPrimary (citable) accession number: P69249
Secondary accession number(s): P00866, P00867, P26666
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 2, 2016
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.