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Protein

Defensin-like protein 1

Gene

AFP1

Organism
Raphanus sativus (Radish)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Possesses antifungal activity sensitive to inorganic cations.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Fungicide

Keywords - Biological processi

Plant defense

Protein family/group databases

TCDBi1.C.45.1.1. the plant defensin (plant defensin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Defensin-like protein 1
Alternative name(s):
Cysteine-rich antifungal protein 1
Short name:
AFP1
Gene namesi
Name:AFP1
OrganismiRaphanus sativus (Radish)
Taxonomic identifieri3726 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeRaphanus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 29291 PublicationAdd
BLAST
Chaini30 – 8051Defensin-like protein 1PRO_0000007034Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei30 – 301Pyrrolidone carboxylic acid
Disulfide bondi33 ↔ 801 Publication
Disulfide bondi44 ↔ 651 Publication
Disulfide bondi50 ↔ 741 Publication
Disulfide bondi54 ↔ 761 Publication

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Interactioni

Subunit structurei

Forms oligomers in its native state.

Structurei

Secondary structure

1
80
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi33 – 364Combined sources
Helixi47 – 5711Combined sources
Beta strandi63 – 664Combined sources
Beta strandi69 – 713Combined sources
Beta strandi73 – 786Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AYJNMR-A31-80[»]
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69241.

Family & Domainsi

Sequence similaritiesi

Belongs to the DEFL family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR008176. Defensin_plant.
IPR003614. Scorpion_toxin-like.
[Graphical view]
SMARTiSM00505. Knot1. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiPS00940. GAMMA_THIONIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P69241-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKFASIIAL LFAALVLFAA FEAPTMVEAQ KLCERPSGTW SGVCGNNNAC
60 70 80
KNQCINLEKA RHGSCNYVFP AHKCICYFPC
Length:80
Mass (Da):8,734
Last modified:February 15, 2005 - v1
Checksum:i05B90FAAC8DA6C2B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18557 mRNA. Translation: AAA69541.1.
PIRiT10176.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18557 mRNA. Translation: AAA69541.1.
PIRiT10176.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AYJNMR-A31-80[»]
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi1.C.45.1.1. the plant defensin (plant defensin) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP69241.

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR008176. Defensin_plant.
IPR003614. Scorpion_toxin-like.
[Graphical view]
SMARTiSM00505. Knot1. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiPS00940. GAMMA_THIONIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Ronde Rode Kleine Witpunt.
    Tissue: Seed.
  2. "Analysis of two novel classes of plant antifungal proteins from radish (Raphanus sativus L.) seeds."
    Terras F.R.G., Schoofs H.M.E., de Bolle M.F.C., van Leuven F., Rees S.B., Vanderleyden J., Cammue B.P.A., Broekaert W.F.
    J. Biol. Chem. 267:15301-15309(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 30-73.
    Tissue: Seed.
  3. "Determination of the three-dimensional solution structure of Raphanus sativus antifungal protein 1 by 1H NMR."
    Fant F., Vranken W.F., Broekaert W.F., Borremans F.A.M.
    J. Mol. Biol. 279:257-270(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 30-80, DISULFIDE BONDS.

Entry informationi

Entry nameiDEF1_RAPSA
AccessioniPrimary (citable) accession number: P69241
Secondary accession number(s): P30225, Q41163
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: February 15, 2005
Last modified: June 8, 2016
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.