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Protein

Defensin-like protein 1

Gene

AFP1

Organism
Raphanus sativus (Radish)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Possesses antifungal activity sensitive to inorganic cations.

GO - Biological processi

Keywordsi

Molecular functionAntimicrobial, Fungicide
Biological processPlant defense

Protein family/group databases

TCDBi1.C.45.1.1. the plant defensin (plant defensin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Defensin-like protein 1
Alternative name(s):
Cysteine-rich antifungal protein 1
Short name:
AFP1
Gene namesi
Name:AFP1
OrganismiRaphanus sativus (Radish)
Taxonomic identifieri3726 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeRaphanus

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 291 PublicationAdd BLAST29
ChainiPRO_000000703430 – 80Defensin-like protein 1Add BLAST51

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei30Pyrrolidone carboxylic acidBy similarity1
Disulfide bondi33 ↔ 801 Publication
Disulfide bondi44 ↔ 651 Publication
Disulfide bondi50 ↔ 741 Publication
Disulfide bondi54 ↔ 761 Publication

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Interactioni

Subunit structurei

Forms oligomers in its native state.

Structurei

Secondary structure

180
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi33 – 36Combined sources4
Helixi47 – 57Combined sources11
Beta strandi63 – 66Combined sources4
Beta strandi69 – 71Combined sources3
Beta strandi73 – 78Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AYJNMR-A31-80[»]
SMRiP69241.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69241.

Family & Domainsi

Sequence similaritiesi

Belongs to the DEFL family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiView protein in InterPro
IPR008176. Defensin_plant.
IPR003614. Scorpion_toxin-like.
SMARTiView protein in SMART
SM00505. Knot1. 1 hit.
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiView protein in PROSITE
PS00940. GAMMA_THIONIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P69241-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKFASIIAL LFAALVLFAA FEAPTMVEAQ KLCERPSGTW SGVCGNNNAC
60 70 80
KNQCINLEKA RHGSCNYVFP AHKCICYFPC
Length:80
Mass (Da):8,734
Last modified:February 15, 2005 - v1
Checksum:i05B90FAAC8DA6C2B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18557 mRNA. Translation: AAA69541.1.
PIRiT10176.
RefSeqiXP_018469135.1. XM_018613633.1.
UniGeneiRsa.21286.

Genome annotation databases

GeneIDi108840814.

Similar proteinsi

Entry informationi

Entry nameiDEF1_RAPSA
AccessioniPrimary (citable) accession number: P69241
Secondary accession number(s): P30225, Q41163
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: February 15, 2005
Last modified: August 30, 2017
This is version 41 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families