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Protein

Defensin-like protein 1

Gene

AFP1

Organism
Raphanus sativus (Radish)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Possesses antifungal activity sensitive to inorganic cations.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antimicrobial, Fungicide

Keywords - Biological processi

Plant defense

Protein family/group databases

TCDBi1.C.45.1.1. the plant defensin (plant defensin) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Defensin-like protein 1
Alternative name(s):
Cysteine-rich antifungal protein 1
Short name:
AFP1
Gene namesi
Name:AFP1
OrganismiRaphanus sativus (Radish)
Taxonomic identifieri3726 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeBrassiceaeRaphanus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 291 PublicationAdd BLAST29
ChainiPRO_000000703430 – 80Defensin-like protein 1Add BLAST51

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei30Pyrrolidone carboxylic acid1
Disulfide bondi33 ↔ 801 Publication
Disulfide bondi44 ↔ 651 Publication
Disulfide bondi50 ↔ 741 Publication
Disulfide bondi54 ↔ 761 Publication

Keywords - PTMi

Disulfide bond, Pyrrolidone carboxylic acid

Interactioni

Subunit structurei

Forms oligomers in its native state.

Structurei

Secondary structure

180
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi33 – 36Combined sources4
Helixi47 – 57Combined sources11
Beta strandi63 – 66Combined sources4
Beta strandi69 – 71Combined sources3
Beta strandi73 – 78Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AYJNMR-A31-80[»]
SMRiP69241.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69241.

Family & Domainsi

Sequence similaritiesi

Belongs to the DEFL family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR008176. Defensin_plant.
IPR003614. Scorpion_toxin-like.
[Graphical view]
SMARTiSM00505. Knot1. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiPS00940. GAMMA_THIONIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P69241-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKFASIIAL LFAALVLFAA FEAPTMVEAQ KLCERPSGTW SGVCGNNNAC
60 70 80
KNQCINLEKA RHGSCNYVFP AHKCICYFPC
Length:80
Mass (Da):8,734
Last modified:February 15, 2005 - v1
Checksum:i05B90FAAC8DA6C2B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18557 mRNA. Translation: AAA69541.1.
PIRiT10176.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U18557 mRNA. Translation: AAA69541.1.
PIRiT10176.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AYJNMR-A31-80[»]
SMRiP69241.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi1.C.45.1.1. the plant defensin (plant defensin) family.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP69241.

Family and domain databases

Gene3Di3.30.30.10. 1 hit.
InterProiIPR008176. Defensin_plant.
IPR003614. Scorpion_toxin-like.
[Graphical view]
SMARTiSM00505. Knot1. 1 hit.
[Graphical view]
SUPFAMiSSF57095. SSF57095. 1 hit.
PROSITEiPS00940. GAMMA_THIONIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDEF1_RAPSA
AccessioniPrimary (citable) accession number: P69241
Secondary accession number(s): P30225, Q41163
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: February 15, 2005
Last modified: November 2, 2016
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.