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Protein

Translation initiation factor IF-1

Gene

infA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

One of the essential components for the initiation of protein synthesis. Binds in the vicinity of the A-site. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre-initiation complex (PIC). Upon addition of the 50S ribosomal subunit, IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initiation complex.2 Publications

GO - Molecular functioni

  • ribosome binding Source: EcoCyc
  • rRNA binding Source: UniProtKB-KW
  • translation initiation factor activity Source: UniProtKB-KW

Keywordsi

Molecular functionInitiation factor, RNA-binding, rRNA-binding
Biological processProtein biosynthesis

Enzyme and pathway databases

BioCyciEcoCyc:EG10504-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Translation initiation factor IF-1UniRule annotation
Gene namesi
Name:infAUniRule annotation
Ordered Locus Names:b0884, JW0867
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10504 infA

Subcellular locationi

  • Cytoplasm UniRule annotation1 Publication

GO - Cellular componenti

  • cytosol Source: EcoCyc

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00000957852 – 72Translation initiation factor IF-1Add BLAST71

Proteomic databases

EPDiP69222
PaxDbiP69222
PRIDEiP69222

Interactioni

Subunit structurei

Component of the 30S ribosomal translation pre-initiation complex which assembles on the 30S ribosome in the order IF-2 and IF-3, IF-1 and N-formylmethionyl-tRNA(fMet); mRNA recruitment can occur at any time during PIC assembly.UniRule annotation2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
mqsAQ468642EBI-1120746,EBI-1120353

Protein-protein interaction databases

BioGridi4262047, 51 interactors
DIPiDIP-48250N
IntActiP69222, 10 interactors
STRINGi316385.ECDH10B_0954

Structurei

Secondary structure

172
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi11 – 16Combined sources6
Beta strandi18 – 26Combined sources9
Beta strandi31 – 36Combined sources6
Helixi40 – 42Combined sources3
Beta strandi64 – 67Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AH9NMR-A2-72[»]
1ZO1electron microscopy13.80W2-70[»]
ProteinModelPortaliP69222
SMRiP69222
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69222

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 72S1-likeUniRule annotationAdd BLAST71

Sequence similaritiesi

Belongs to the IF-1 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0361 LUCA
HOGENOMiHOG000221323
InParanoidiP69222
KOiK02518
OMAiAHVSGKM
PhylomeDBiP69222

Family and domain databases

CDDicd04451 S1_IF1, 1 hit
HAMAPiMF_00075 IF_1, 1 hit
InterProiView protein in InterPro
IPR012340 NA-bd_OB-fold
IPR006196 RNA-binding_domain_S1_IF1
IPR022967 S1_dom
IPR004368 TIF_IF1
PANTHERiPTHR33370 PTHR33370, 1 hit
PfamiView protein in Pfam
PF01176 eIF-1a, 1 hit
SMARTiView protein in SMART
SM00316 S1, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
TIGRFAMsiTIGR00008 infA, 1 hit
PROSITEiView protein in PROSITE
PS50832 S1_IF1_TYPE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P69222-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKEDNIEMQ GTVLETLPNT MFRVELENGH VVTAHISGKM RKNYIRILTG
60 70
DKVTVELTPY DLSKGRIVFR SR
Length:72
Mass (Da):8,250
Last modified:January 23, 2007 - v2
Checksum:iCDFE0845C4CBE147
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00373 Genomic DNA Translation: CAA68446.1
M63145 Genomic DNA Translation: AAC36912.1 Sequence problems.
L10383 Unassigned DNA Translation: AAA03232.1
U00096 Genomic DNA Translation: AAC73970.1
AP009048 Genomic DNA Translation: BAA35602.1
PIRiA27855 FIEC1
RefSeqiNP_415404.1, NC_000913.3
WP_001040187.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73970; AAC73970; b0884
BAA35602; BAA35602; BAA35602
GeneIDi35808257
945500
KEGGiecj:JW0867
eco:b0884
PATRICifig|1411691.4.peg.1394

Similar proteinsi

Entry informationi

Entry nameiIF1_ECOLI
AccessioniPrimary (citable) accession number: P69222
Secondary accession number(s): P02998
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: April 25, 2018
This is version 118 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Translation initiation factors
    List of translation initiation factor entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

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