Reviewed, UniProtKB/Swiss-Prot P69222 (IF1_ECOLI)

Names and origin

Protein names

Recommended name:
    Translation initiation factor IF-1

Gene names

Name:	infA
Ordered Locus Names:	b0884, JW0867

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	72 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	No specific function has so far been attributed to this initiation factor; however, it seems to stimulate more or less all the activities of the other two initiation factors, IF-2 and IF-3. HAMAP MF_00075
Subcellular location	Cytoplasm. HAMAP MF_00075
Sequence similarities	Belongs to the IF-1 family. Contains 1 S1-like domain.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Molecular function	Initiation factor
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translational initiation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: HAMAP translation initiation factor activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.7 Ref.8

Chain

2 – 72

71

Translation initiation factor IF-1 HAMAP MF_00075

PRO_0000095785

Regions

Domain

2 – 72

71

S1-like

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

72

Helix Strand Turn

Details...

Beta strand

11 – 16

6

Beta strand

18 – 26

9

Beta strand

31 – 36

6

Helix

40 – 42

3

Beta strand

64 – 67

4

Sequences

	Sequence		Length	Mass (Da)
	P69222-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: CDFE0845C4CBE147	FASTA	72	8,250
	10 20 30 40 50 60 MAKEDNIEMQ GTVLETLPNT MFRVELENGH VVTAHISGKM RKNYIRILTG DKVTVELTPY 70 DLSKGRIVFR SR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and mapping of infA, the gene for protein synthesis initiation factor IF1." Sands J.F., Cummings H.S., Sacerdot C., Dondon L., Grunberg-Manago M., Hershey J.W.B. Nucleic Acids Res. 15:5157-5168(1987) [PubMed: 3037488] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Structure and expression of the infA operon encoding translational initiation factor IF1. Transcriptional control by growth rate." Cummings H.S., Sands J.F., Foreman P.C., Fraser J., Hershey J.W.B. J. Biol. Chem. 266:16491-16498(1991) [PubMed: 1909328] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The N-end rule in Escherichia coli: cloning and analysis of the leucyl, phenylalanyl-tRNA-protein transferase gene aat." Shrader T.E., Tobias J.W., Varshavsky A. J. Bacteriol. 175:4364-4374(1993) [PubMed: 8331068] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12 / MC1061 / ATCC 53338 / DSM 7140.
[4]	"A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K., Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S., Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K. , Mori H., Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G., Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M., Horiuchi T. DNA Res. 3:137-155(1996) [PubMed: 8905232] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[6]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[7]	"Structure-function relationships in Escherichia coli initiation factors. II. Elucidation of the primary structure of initiation factor IF-1." Pon C.L., Wittmann-Liebold B., Gualerzi C. FEBS Lett. 101:157-160(1979) [PubMed: 376343] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-72. Strain: MRE-600.
[8]	"Small genes/gene-products in Escherichia coli K-12." Wasinger V.C., Humphery-Smith I. FEMS Microbiol. Lett. 169:375-382(1998) [PubMed: 9868784] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-11. Strain: K12.
[9]	"The structure of the translational initiation factor IF1 from E.coli contains an oligomer-binding motif." Sette M., van Tilborg P., Spurio R., Kaptein R., Paci M., Gualerzi C.O., Boelens R. EMBO J. 16:1436-1443(1997) [PubMed: 9135158] [Abstract] Cited for: STRUCTURE BY NMR.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

Y00373 Genomic DNA. Translation: CAA68446.1.
M63145 Genomic DNA. Translation: AAC36912.1. Sequence problems.
L10383 Unassigned DNA. Translation: AAA03232.1.
U00096 Genomic DNA. Translation: AAC73970.1.
AP009048 Genomic DNA. Translation: BAA35602.1.

PIR

FIEC1. A27855.

RefSeq

AP_001514.1.
NP_415404.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1AH9	NMR	-	A	2-72	[»]
1ZO1	electron microscopy	13.80	W	22-61	[»]

ModBase

Search...

PTM databases

PhosSite

P69222.

Genome annotation databases

GeneID

945500.

GenomeReviews

Gene locus JW0867 in contig AP009048_GR.
Gene locus b0884 in contig U00096_GR.

KEGG

ecj:JW0867.
eco:b0884.

Organism-specific databases

EchoBASE

EB0499.

EcoGene

EG10504. infA.

CMR

Search...

Phylogenomic databases

HOGENOM

P69222.

OMA

P69222. KEDSIEM.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10504-MON.

Family and domain databases

HAMAP

MF_00075.
[Tree]

InterPro

IPR006196. S1_IF1.
IPR004368. TIF_IF1.
[Graphical view]

Pfam

PF01176. eIF-1a. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00008. infA. 1 hit.

PROSITE

PS50832. S1_IF1_TYPE. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

IF1_ECOLI

Accession

Primary (citable) accession number: P69222
Secondary accession number(s): P02998

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 46 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)