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Protein

Translation initiation factor IF-1

Gene

infA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

One of the essential components for the initiation of protein synthesis. Binds in the vicinity of the A-site. Stabilizes the binding of IF-2 and IF-3 on the 30S subunit to which N-formylmethionyl-tRNA(fMet) subsequently binds. Helps modulate mRNA selection, yielding the 30S pre-initiation complex (PIC). Upon addition of the 50S ribosomal subunit, IF-1, IF-2 and IF-3 are released leaving the mature 70S translation initation complex.2 Publications

GO - Molecular functioni

  • ribosome binding Source: EcoCyc
  • rRNA binding Source: UniProtKB-HAMAP
  • translation initiation factor activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Initiation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

RNA-binding, rRNA-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG10504-MONOMER.
ECOL316407:JW0867-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Translation initiation factor IF-1UniRule annotation
Gene namesi
Name:infAUniRule annotation
Ordered Locus Names:b0884, JW0867
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10504. infA.

Subcellular locationi

  • Cytoplasm UniRule annotation1 Publication

GO - Cellular componenti

  • cytosol Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved2 Publications
Chaini2 – 7271Translation initiation factor IF-1PRO_0000095785Add
BLAST

Proteomic databases

EPDiP69222.
PaxDbiP69222.
PRIDEiP69222.

Interactioni

Subunit structurei

Component of the 30S ribosomal translation pre-initiation complex which assembles on the 30S ribosome in the order IF-2 and IF-3, IF-1 and N-formylmethionyl-tRNA(fMet); mRNA recruitment can occur at any time during PIC assembly.UniRule annotation2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
mqsAQ468642EBI-1120746,EBI-1120353

Protein-protein interaction databases

BioGridi4262047. 10 interactions.
DIPiDIP-48250N.
IntActiP69222. 10 interactions.
STRINGi511145.b0884.

Structurei

Secondary structure

1
72
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi11 – 166Combined sources
Beta strandi18 – 269Combined sources
Beta strandi31 – 366Combined sources
Helixi40 – 423Combined sources
Beta strandi64 – 674Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AH9NMR-A2-72[»]
1ZO1electron microscopy13.80W34-61[»]
ProteinModelPortaliP69222.
SMRiP69222. Positions 2-72.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69222.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 7271S1-likeUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the IF-1 family.UniRule annotation
Contains 1 S1-like domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0361. LUCA.
HOGENOMiHOG000221323.
InParanoidiP69222.
KOiK02518.
OMAiKGRIIWR.
PhylomeDBiP69222.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00075. IF_1. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR006196. RNA-binding_domain_S1_IF1.
IPR022967. S1_dom.
IPR004368. TIF_IF1.
[Graphical view]
PfamiPF01176. eIF-1a. 1 hit.
[Graphical view]
SMARTiSM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00008. infA. 1 hit.
PROSITEiPS50832. S1_IF1_TYPE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P69222-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKEDNIEMQ GTVLETLPNT MFRVELENGH VVTAHISGKM RKNYIRILTG
60 70
DKVTVELTPY DLSKGRIVFR SR
Length:72
Mass (Da):8,250
Last modified:January 23, 2007 - v2
Checksum:iCDFE0845C4CBE147
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00373 Genomic DNA. Translation: CAA68446.1.
M63145 Genomic DNA. Translation: AAC36912.1. Sequence problems.
L10383 Unassigned DNA. Translation: AAA03232.1.
U00096 Genomic DNA. Translation: AAC73970.1.
AP009048 Genomic DNA. Translation: BAA35602.1.
PIRiA27855. FIEC1.
RefSeqiNP_415404.1. NC_000913.3.
WP_001040187.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73970; AAC73970; b0884.
BAA35602; BAA35602; BAA35602.
GeneIDi5552070.
945500.
KEGGiecj:JW0867.
eco:b0884.
PATRICi32116977. VBIEscCol129921_0913.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00373 Genomic DNA. Translation: CAA68446.1.
M63145 Genomic DNA. Translation: AAC36912.1. Sequence problems.
L10383 Unassigned DNA. Translation: AAA03232.1.
U00096 Genomic DNA. Translation: AAC73970.1.
AP009048 Genomic DNA. Translation: BAA35602.1.
PIRiA27855. FIEC1.
RefSeqiNP_415404.1. NC_000913.3.
WP_001040187.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AH9NMR-A2-72[»]
1ZO1electron microscopy13.80W34-61[»]
ProteinModelPortaliP69222.
SMRiP69222. Positions 2-72.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262047. 10 interactions.
DIPiDIP-48250N.
IntActiP69222. 10 interactions.
STRINGi511145.b0884.

Proteomic databases

EPDiP69222.
PaxDbiP69222.
PRIDEiP69222.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73970; AAC73970; b0884.
BAA35602; BAA35602; BAA35602.
GeneIDi5552070.
945500.
KEGGiecj:JW0867.
eco:b0884.
PATRICi32116977. VBIEscCol129921_0913.

Organism-specific databases

EchoBASEiEB0499.
EcoGeneiEG10504. infA.

Phylogenomic databases

eggNOGiCOG0361. LUCA.
HOGENOMiHOG000221323.
InParanoidiP69222.
KOiK02518.
OMAiKGRIIWR.
PhylomeDBiP69222.

Enzyme and pathway databases

BioCyciEcoCyc:EG10504-MONOMER.
ECOL316407:JW0867-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP69222.
PROiP69222.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
HAMAPiMF_00075. IF_1. 1 hit.
InterProiIPR012340. NA-bd_OB-fold.
IPR006196. RNA-binding_domain_S1_IF1.
IPR022967. S1_dom.
IPR004368. TIF_IF1.
[Graphical view]
PfamiPF01176. eIF-1a. 1 hit.
[Graphical view]
SMARTiSM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
TIGRFAMsiTIGR00008. infA. 1 hit.
PROSITEiPS50832. S1_IF1_TYPE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIF1_ECOLI
AccessioniPrimary (citable) accession number: P69222
Secondary accession number(s): P02998
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. Translation initiation factors
    List of translation initiation factor entries
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.