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Protein

Spermidine export protein MdtJ

Gene

mdtJ

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the excretion of spermidine. Can also confer resistance to deoxycholate and SDS.2 Publications

GO - Molecular functioni

  • spermidine transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • spermidine transmembrane transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:B1600-MONOMER.
ECOL316407:JW1592-MONOMER.
MetaCyc:B1600-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Spermidine export protein MdtJ
Gene namesi
Name:mdtJ
Synonyms:ydgF
Ordered Locus Names:b1600, JW1592
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13927. mdtJ.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 11CytoplasmicSequence analysis
Transmembranei2 – 2221HelicalSequence analysisAdd
BLAST
Topological domaini23 – 319PeriplasmicSequence analysis
Transmembranei32 – 5221HelicalSequence analysisAdd
BLAST
Topological domaini53 – 542CytoplasmicSequence analysis
Transmembranei55 – 7521HelicalSequence analysisAdd
BLAST
Topological domaini76 – 816PeriplasmicSequence analysis
Transmembranei82 – 10221HelicalSequence analysisAdd
BLAST
Topological domaini103 – 12119CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi4 – 41Y → L: Decrease in spermidine excretion. 1 Publication
Mutagenesisi5 – 51W → L: Decrease in spermidine excretion. 1 Publication
Mutagenesisi15 – 151E → Q: Decrease in spermidine excretion. 1 Publication
Mutagenesisi45 – 451Y → L: Decrease in spermidine excretion. 1 Publication
Mutagenesisi61 – 611Y → L: Decrease in spermidine excretion. 1 Publication
Mutagenesisi82 – 821E → Q: Decrease in spermidine excretion. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 121121Spermidine export protein MdtJPRO_0000108079Add
BLAST

Proteomic databases

PaxDbiP69212.

Expressioni

Inductioni

Induced by spermidine.1 Publication

Interactioni

Subunit structurei

Forms a complex with MdtI.1 Publication

Protein-protein interaction databases

BioGridi4260838. 195 interactions.
STRINGi511145.b1600.

Structurei

3D structure databases

ProteinModelPortaliP69212.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG41090TI. Bacteria.
COG2076. LUCA.
HOGENOMiHOG000268006.
InParanoidiP69212.
KOiK11743.
OMAiYALWEGV.
PhylomeDBiP69212.

Family and domain databases

HAMAPiMF_01598. MdtJ. 1 hit.
InterProiIPR000390. Small_multidrug_res.
IPR023740. Spermidine_export_MdtJ.
[Graphical view]
PANTHERiPTHR30561:SF2. PTHR30561:SF2. 1 hit.
PfamiPF00893. Multi_Drug_Res. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P69212-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYIYWILLGL AIATEITGTL SMKWASVSEG NGGFILMLVM ISLSYIFLSF
60 70 80 90 100
AVKKIALGVA YALWEGIGIL FITLFSVLLF DESLSLMKIA GLTTLVAGIV
110 120
LIKSGTRKAR KPELEVNHGA V
Length:121
Mass (Da):13,115
Last modified:February 15, 2005 - v1
Checksum:iB24330AF5BB181B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74672.1.
AP009048 Genomic DNA. Translation: BAA15334.1.
PIRiB64916.
RefSeqiNP_416117.1. NC_000913.3.
WP_000276149.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74672; AAC74672; b1600.
BAA15334; BAA15334; BAA15334.
GeneIDi946139.
KEGGiecj:JW1592.
eco:b1600.
PATRICi32118502. VBIEscCol129921_1671.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74672.1.
AP009048 Genomic DNA. Translation: BAA15334.1.
PIRiB64916.
RefSeqiNP_416117.1. NC_000913.3.
WP_000276149.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP69212.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4260838. 195 interactions.
STRINGi511145.b1600.

Proteomic databases

PaxDbiP69212.

Protocols and materials databases

DNASUi946139.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74672; AAC74672; b1600.
BAA15334; BAA15334; BAA15334.
GeneIDi946139.
KEGGiecj:JW1592.
eco:b1600.
PATRICi32118502. VBIEscCol129921_1671.

Organism-specific databases

EchoBASEiEB3686.
EcoGeneiEG13927. mdtJ.

Phylogenomic databases

eggNOGiENOG41090TI. Bacteria.
COG2076. LUCA.
HOGENOMiHOG000268006.
InParanoidiP69212.
KOiK11743.
OMAiYALWEGV.
PhylomeDBiP69212.

Enzyme and pathway databases

BioCyciEcoCyc:B1600-MONOMER.
ECOL316407:JW1592-MONOMER.
MetaCyc:B1600-MONOMER.

Miscellaneous databases

PROiP69212.

Family and domain databases

HAMAPiMF_01598. MdtJ. 1 hit.
InterProiIPR000390. Small_multidrug_res.
IPR023740. Spermidine_export_MdtJ.
[Graphical view]
PANTHERiPTHR30561:SF2. PTHR30561:SF2. 1 hit.
PfamiPF00893. Multi_Drug_Res. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMDTJ_ECOLI
AccessioniPrimary (citable) accession number: P69212
Secondary accession number(s): P77412
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: February 15, 2005
Last modified: September 7, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.