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Protein

Spermidine export protein MdtI

Gene

mdtI

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the excretion of spermidine. Can also confer resistance to deoxycholate and SDS.2 Publications

GO - Molecular functioni

  • spermidine transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • spermidine transmembrane transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:B1599-MONOMER.
ECOL316407:JW1591-MONOMER.
MetaCyc:B1599-MONOMER.

Protein family/group databases

TCDBi2.A.7.1.9. the drug/metabolite transporter (dmt) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Spermidine export protein MdtI
Gene namesi
Name:mdtI
Synonyms:ydgE
Ordered Locus Names:b1599, JW1591
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG13926. mdtI.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 55PeriplasmicSequence analysis
Transmembranei6 – 2621HelicalSequence analysisAdd
BLAST
Topological domaini27 – 359CytoplasmicSequence analysis
Transmembranei36 – 5621HelicalSequence analysisAdd
BLAST
Topological domaini57 – 637PeriplasmicSequence analysis
Transmembranei64 – 8421HelicalSequence analysisAdd
BLAST
Topological domaini85 – 873CytoplasmicSequence analysis
Transmembranei88 – 10821HelicalSequence analysisAdd
BLAST
Topological domaini109 – 1091PeriplasmicSequence analysis

GO - Cellular componenti

  • integral component of plasma membrane Source: EcoliWiki
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi5 – 51E → Q: Decrease in spermidine excretion. 1 Publication
Mutagenesisi19 – 191E → Q: Decrease in spermidine excretion. 1 Publication
Mutagenesisi60 – 601D → N: Decrease in spermidine excretion. 1 Publication
Mutagenesisi68 – 681W → L: Decrease in spermidine excretion. 1 Publication
Mutagenesisi81 – 811W → L: Decrease in spermidine excretion. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 109109Spermidine export protein MdtIPRO_0000108075Add
BLAST

Proteomic databases

PaxDbiP69210.

Expressioni

Inductioni

Induced by spermidine.1 Publication

Interactioni

Subunit structurei

Forms a complex with MdtJ.1 Publication

Protein-protein interaction databases

BioGridi4259124. 494 interactions.
IntActiP69210. 1 interaction.
STRINGi511145.b1599.

Structurei

3D structure databases

ProteinModelPortaliP69210.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108ZBZ. Bacteria.
COG2076. LUCA.
HOGENOMiHOG000268006.
InParanoidiP69210.
KOiK11742.
OMAiFRRKFYG.
OrthoDBiEOG6K13QJ.
PhylomeDBiP69210.

Family and domain databases

HAMAPiMF_01597. MdtI.
InterProiIPR000390. Small_multidrug_res.
IPR023737. Spermidine_export_MdtI.
[Graphical view]
PANTHERiPTHR30561:SF6. PTHR30561:SF6. 1 hit.
PfamiPF00893. Multi_Drug_Res. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P69210-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQFEWVHAA WLALAIVLEI VANVFLKFSD GFRRKIFGLL SLAAVLAAFS
60 70 80 90 100
ALSQAVKGID LSVAYALWGG FGIAATLAAG WILFGQRLNR KGWIGLVLLL

AGMIMVKLA
Length:109
Mass (Da):11,720
Last modified:February 15, 2005 - v1
Checksum:iBBDF61985CB7AE97
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74671.1.
AP009048 Genomic DNA. Translation: BAA15333.1.
PIRiA64916.
RefSeqiNP_416116.1. NC_000913.3.
WP_000046661.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74671; AAC74671; b1599.
BAA15333; BAA15333; BAA15333.
GeneIDi947333.
KEGGiecj:JW1591.
eco:b1599.
PATRICi32118500. VBIEscCol129921_1670.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74671.1.
AP009048 Genomic DNA. Translation: BAA15333.1.
PIRiA64916.
RefSeqiNP_416116.1. NC_000913.3.
WP_000046661.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP69210.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259124. 494 interactions.
IntActiP69210. 1 interaction.
STRINGi511145.b1599.

Protein family/group databases

TCDBi2.A.7.1.9. the drug/metabolite transporter (dmt) superfamily.

Proteomic databases

PaxDbiP69210.

Protocols and materials databases

DNASUi947333.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74671; AAC74671; b1599.
BAA15333; BAA15333; BAA15333.
GeneIDi947333.
KEGGiecj:JW1591.
eco:b1599.
PATRICi32118500. VBIEscCol129921_1670.

Organism-specific databases

EchoBASEiEB3685.
EcoGeneiEG13926. mdtI.

Phylogenomic databases

eggNOGiENOG4108ZBZ. Bacteria.
COG2076. LUCA.
HOGENOMiHOG000268006.
InParanoidiP69210.
KOiK11742.
OMAiFRRKFYG.
OrthoDBiEOG6K13QJ.
PhylomeDBiP69210.

Enzyme and pathway databases

BioCyciEcoCyc:B1599-MONOMER.
ECOL316407:JW1591-MONOMER.
MetaCyc:B1599-MONOMER.

Miscellaneous databases

PROiP69210.

Family and domain databases

HAMAPiMF_01597. MdtI.
InterProiIPR000390. Small_multidrug_res.
IPR023737. Spermidine_export_MdtI.
[Graphical view]
PANTHERiPTHR30561:SF6. PTHR30561:SF6. 1 hit.
PfamiPF00893. Multi_Drug_Res. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Analysis of a complete library of putative drug transporter genes in Escherichia coli."
    Nishino K., Yamaguchi A.
    J. Bacteriol. 183:5803-5812(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Rapid topology mapping of Escherichia coli inner-membrane proteins by prediction and PhoA/GFP fusion analysis."
    Drew D., Sjoestrand D., Nilsson J., Urbig T., Chin C.-N., de Gier J.-W., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 99:2690-2695(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY.
    Strain: K12 / JM109 / ATCC 53323.
  6. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.
  7. "Identification of a spermidine excretion protein complex (MdtJI) in Escherichia coli."
    Higashi K., Ishigure H., Demizu R., Uemura T., Nishino K., Yamaguchi A., Kashiwagi K., Igarashi K.
    J. Bacteriol. 190:872-878(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, INDUCTION, MUTAGENESIS OF GLU-5; GLU-19; ASP-60; TRP-68 AND TRP-81.
    Strain: K12 / C600 / ATCC 23724 / DSM 3925 / LMG 3041 / NCIB 10222.

Entry informationi

Entry nameiMDTI_ECOLI
AccessioniPrimary (citable) accession number: P69210
Secondary accession number(s): P77670
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 15, 2005
Last sequence update: February 15, 2005
Last modified: April 13, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.