P69186 (KITH_EHV1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
EC=2.7.1.21

Gene names

Name:

TK

Organism

Equine herpesvirus 1 (strain HVS25A) (EHV-1) (Equine abortion virus)

Taxonomic identifier

10327 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Alphaherpesvirinae › Varicellovirus › Equid herpesvirus 1

Virus host

Equus caballus (Horse) [TaxID: 9796]

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate.
Sequence similarities	Belongs to the herpesviruses thymidine kinase family.

Ontologies

Keywords
Biological process	DNA synthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW TMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW thymidine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 352	352	Thymidine kinase		PRO_0000175065
Regions
	Nucleotide binding	32 – 39	8	ATP By similarity
Sites
	Active site	60	1	Proton acceptor Potential
	Binding site	78	1	Substrate By similarity
	Binding site	102	1	Substrate By similarity
	Binding site	148	1	Substrate By similarity
	Binding site	192	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P69186 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 66EF05158DD3B3F9	FASTA	352	38,750
	10 20 30 40 50 60 MAARVPSGEA RRSASGAPVR RQVTIVRIYL DGVYGIGKST TGRVMASAAS GGSPTLYFPE 70 80 90 100 110 120 PMAYWRTLFE ADVISGIYDT QNRKQQGDLA ADDAASITAH YQSRFTTPYL ILHDHTFGLF 130 140 150 160 170 180 GGDSLQRGTR PDLTVVFDRH PVASAVCFPA ARYLIGDMSM CALIAMVATL PREPQGGNIV 190 200 210 220 230 240 VTTLNVDEHV RRLRTRARIG EQIDMKLIAT LRNVYSMLAN TSNFLRSGRV WRDGWGELPL 250 260 270 280 290 300 SCETYKHRAT QMDAFQERES PELSDTLFAM FKTPELLDDR GVILEVHAWA LDALMLKLRN 310 320 330 340 350 LSVFCADLSG TPRQCAATVE SLIPLMSSTL SDSESASSLE RAARTFNAEM GV

References

[1]

"Evolution of the herpes thymidine kinase: identification and comparison of the equine herpesvirus 1 thymidine kinase gene reveals similarity to a cell-encoded thymidylate kinase." Robertson G.R., Whalley J.M. Nucleic Acids Res. 16:11303-11317(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	X13209 Genomic DNA. Translation: CAA31599.1.
PIR	KIBED2. S01995.
RefSeq	YP_053084.1. NC_001491.2.
3D structure databases
ProteinModelPortal	P69186.
SMR	P69186. Positions 23-352.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1487533.
Phylogenomic databases
ProtClustDB	PHA3134.
Family and domain databases
InterPro	IPR001889. Herpes_TK. [Graphical view]
Pfam	PF00693. Herpes_TK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KITH_EHV1

Accession

Primary (citable) accession number: P69186
Secondary accession number(s): P09100, P28856

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	February 15, 2005
Last modified:	April 3, 2013
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program