P69167 (HSD_MYCTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase
EC=1.1.1.53

Gene names

Name:	fabG3
Ordered Locus Names:	Rv2002, MT2058
ORF Names:	MTCY39.16c

Organism

Mycobacterium tuberculosis

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Androstan-3-alpha,17-beta-diol + NAD+ = 17-beta-hydroxyandrostan-3-one + NADH.
Pathway	Lipid metabolism; C21-steroid hormone metabolism.
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Lipid metabolism Steroid metabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	steroid metabolic process Inferred from direct assay Ref.3. Source: MTBBASE
Cellular component	plasma membrane Inferred from direct assay. Source: MTBBASE
Molecular function	androstan-3-alpha,17-beta-diol dehydrogenase activity Inferred from direct assay Ref.3. Source: MTBBASE nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 260

260

3-alpha-(or 20-beta)-hydroxysteroid dehydrogenase

PRO_0000054712

Regions

Nucleotide binding

11 – 35

25

NAD By similarity

Sites

Active site

153

1

Proton acceptor

Binding site

140

1

Substrate

Experimental info

Sequence conflict

174

1

S → G in AAK46335. Ref.2

Secondary structure

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260

Helix Strand Turn

Details...

Turn

4 – 7

4

Beta strand

9 – 13

5

Turn

14 – 16

3

Helix

18 – 29

12

Beta strand

33 – 39

7

Helix

41 – 50

10

Helix

52 – 54

3

Beta strand

55 – 59

5

Helix

65 – 79

15

Beta strand

84 – 87

4

Turn

97 – 99

3

Helix

102 – 112

11

Helix

114 – 130

17

Beta strand

133 – 138

6

Helix

141 – 143

3

Helix

151 – 171

21

Helix

172 – 174

3

Beta strand

176 – 183

8

Helix

189 – 191

3

Beta strand

202 – 205

4

Helix

209 – 220

12

Helix

222 – 224

3

Beta strand

231 – 235

5

Helix

238 – 240

3

Sequences

	Sequence		Length	Mass (Da)
	P69167 [UniParc]. Last modified February 15, 2005. Version 1. Checksum: 0935A14ED36220B7	FASTA	260	27,030
	10 20 30 40 50 60 MSGRLIGKVA LVSGGARGMG ASHVRAMVAE GAKVVFGDIL DEEGKAVAAE LADAARYVHL 70 80 90 100 110 120 DVTQPAQWTA AVDTAVTAFG GLHVLVNNAG ILNIGTIEDY ALTEWQRILD VNLTGVFLGI 130 140 150 160 170 180 RAVVKPMKEA GRGSIINISS IEGLAGTVAC HGYTATKFAV RGLTKSTALE LGPSGIRVNS 190 200 210 220 230 240 IHPGLVKTPM TDWVPEDIFQ TALGRAAEPV EVSNLVVYLA SDESSYSTGA EFVVDGGTVA 250 260 GLAHNDFGAV EVSSQPEWVT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[3]	"Directed evolution approach to a structural genomics project: Rv2002 from Mycobacterium tuberculosis." Yang J.K., Park M.S., Waldo G.S., Suh S.W. Proc. Natl. Acad. Sci. U.S.A. 100:455-460(2003) [PubMed: 12524453] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF MUTANTS THR-6; MET-47 AND LYS-69. Strain: ATCC 25618 / H37Rv.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

BX842578 Genomic DNA. Translation: CAA98414.1.
AE000516 Genomic DNA. Translation: AAK46335.1.

PIR

H70758.

RefSeq

NP_216518.1. NC_000962.2.
NP_336521.1. NC_002755.2.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1NFF	X-ray	1.80	A/B	1-260	[»]
1NFQ	X-ray	2.40	A/B/C/D	1-260	[»]
1NFR	X-ray	2.10	A/B/C/D	1-260	[»]

ProteinModelPortal

P69167.

SMR

P69167. Positions 2-245.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBMYCT00000001684; EBMYCP00000001684; EBMYCG00000001683.
EBMYCT00000072037; EBMYCP00000070096; EBMYCG00000072032.

GeneID

888857.
923379.

GenomeReviews

Gene locus MT2058 in contig AE000516_GR.
Gene locus Rv2002 in contig AL123456_GR.

KEGG

mtc:MT2058.
mtu:Rv2002.

PATRIC

18126286. VBIMycTub22151_2255.

TIGR

MT2058.

Organism-specific databases

TubercuList

Rv2002.

Phylogenomic databases

GeneTree

EBGT00050000015165.

HOGENOM

HBG750976.

OMA

WQRILDI.

PhylomeDB

P69167.

ProtClustDB

CLSK871992.

Family and domain databases

InterPro

IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]

Gene3D

G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.

KO

K00038.

Pfam

PF00106. adh_short. 1 hit.
[Graphical view]

PRINTS

PR00081. GDHRDH.
PR00080. SDRFAMILY.

PROSITE

PS00061. ADH_SHORT. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

HSD_MYCTU

Accession

Primary (citable) accession number: P69167
Secondary accession number(s): Q10855

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 15, 2005
Last sequence update:	February 15, 2005
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program