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Protein

Succinate dehydrogenase cytochrome b556 subunit

Gene

sdhC

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Membrane-anchoring subunit of succinate dehydrogenase (SDH).

Cofactori

hemeNote: The heme is bound between the two transmembrane subunits.

Pathwayi: tricarboxylic acid cycle

This protein is involved in the pathway tricarboxylic acid cycle, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi84 – 841Iron (heme axial ligand); shared with second transmembrane subunit

GO - Molecular functioni

  • electron carrier activity Source: EcoCyc
  • heme binding Source: EcoCyc
  • metal ion binding Source: UniProtKB-KW
  • succinate dehydrogenase (ubiquinone) activity Source: EcoliWiki
  • ubiquinone binding Source: EcoliWiki

GO - Biological processi

  • aerobic respiration Source: EcoCyc
  • cytochrome complex assembly Source: EcoCyc
  • tricarboxylic acid cycle Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport, Tricarboxylic acid cycle

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:SDH-MEMB1.
ECOL316407:JW0711-MONOMER.
MetaCyc:SDH-MEMB1.
UniPathwayiUPA00223.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate dehydrogenase cytochrome b556 subunit
Short name:
Cytochrome b-556
Gene namesi
Name:sdhC
Synonyms:cybA
Ordered Locus Names:b0721, JW0711
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10933. sdhC.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2626Cytoplasmic1 PublicationAdd
BLAST
Transmembranei27 – 5226HelicalAdd
BLAST
Topological domaini53 – 6816Periplasmic1 PublicationAdd
BLAST
Transmembranei69 – 8921HelicalAdd
BLAST
Topological domaini90 – 10819Cytoplasmic1 PublicationAdd
BLAST
Transmembranei109 – 12921HelicalAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 129129Succinate dehydrogenase cytochrome b556 subunitPRO_0000203510Add
BLAST

Proteomic databases

PaxDbiP69054.

Interactioni

Subunit structurei

Part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur protein, plus two membrane-anchoring proteins, SdhC and SdhD. The complex can form homotrimers.3 Publications

Protein-protein interaction databases

BioGridi4259942. 11 interactions.
DIPiDIP-10837N.
IntActiP69054. 4 interactions.
STRINGi511145.b0721.

Structurei

Secondary structure

1
129
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi15 – 173Combined sources
Helixi22 – 5231Combined sources
Helixi55 – 6612Combined sources
Helixi68 – 9528Combined sources
Helixi103 – 12624Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NEKX-ray2.60C1-129[»]
1NENX-ray2.90C1-129[»]
2ACZX-ray3.10C1-129[»]
2AD0model-C1-129[»]
2WDQX-ray2.40C/G/K1-129[»]
2WDRX-ray3.20C/G/K1-129[»]
2WDVX-ray3.20C/G/K1-129[»]
2WP9X-ray2.70C/G/K1-129[»]
2WS3X-ray3.20C/G/K1-129[»]
2WU2X-ray2.50C/G/K1-129[»]
2WU5X-ray2.80C/G/K1-129[»]
ProteinModelPortaliP69054.
SMRiP69054. Positions 8-128.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP69054.

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome b560 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108UTY. Bacteria.
COG2009. LUCA.
HOGENOMiHOG000160252.
InParanoidiP69054.
KOiK00241.
OMAiIVKFIVW.
PhylomeDBiP69054.

Family and domain databases

InterProiIPR018495. Succ_DH_cyt_bsu_CS.
IPR014314. Succ_DH_cytb556.
IPR000701. Succ_DH_Fumarate_Rdtase_TM-su.
[Graphical view]
PfamiPF01127. Sdh_cyt. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02970. succ_dehyd_cytB. 1 hit.
PROSITEiPS01000. SDH_CYT_1. 1 hit.
PS01001. SDH_CYT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P69054-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIRNVKKQRP VNLDLQTIRF PITAIASILH RVSGVITFVA VGILLWLLGT
60 70 80 90 100
SLSSPEGFEQ ASAIMGSFFV KFIMWGILTA LAYHVVVGIR HMMMDFGYLE
110 120
ETFEAGKRSA KISFVITVVL SLLAGVLVW
Length:129
Mass (Da):14,299
Last modified:February 1, 2005 - v1
Checksum:i57485D25D2535818
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01619 Genomic DNA. Translation: AAA23893.1.
M28989 Genomic DNA. Translation: AAA24616.1.
X00980 Genomic DNA. Translation: CAA25485.1.
U00096 Genomic DNA. Translation: AAC73815.1.
AP009048 Genomic DNA. Translation: BAA35388.1.
PIRiA28836. DEECS4.
RefSeqiNP_415249.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC73815; AAC73815; b0721.
BAA35388; BAA35388; BAA35388.
GeneIDi945316.
KEGGiecj:JW0711.
eco:b0721.
PATRICi32116637. VBIEscCol129921_0751.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01619 Genomic DNA. Translation: AAA23893.1.
M28989 Genomic DNA. Translation: AAA24616.1.
X00980 Genomic DNA. Translation: CAA25485.1.
U00096 Genomic DNA. Translation: AAC73815.1.
AP009048 Genomic DNA. Translation: BAA35388.1.
PIRiA28836. DEECS4.
RefSeqiNP_415249.1. NC_000913.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NEKX-ray2.60C1-129[»]
1NENX-ray2.90C1-129[»]
2ACZX-ray3.10C1-129[»]
2AD0model-C1-129[»]
2WDQX-ray2.40C/G/K1-129[»]
2WDRX-ray3.20C/G/K1-129[»]
2WDVX-ray3.20C/G/K1-129[»]
2WP9X-ray2.70C/G/K1-129[»]
2WS3X-ray3.20C/G/K1-129[»]
2WU2X-ray2.50C/G/K1-129[»]
2WU5X-ray2.80C/G/K1-129[»]
ProteinModelPortaliP69054.
SMRiP69054. Positions 8-128.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259942. 11 interactions.
DIPiDIP-10837N.
IntActiP69054. 4 interactions.
STRINGi511145.b0721.

Proteomic databases

PaxDbiP69054.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73815; AAC73815; b0721.
BAA35388; BAA35388; BAA35388.
GeneIDi945316.
KEGGiecj:JW0711.
eco:b0721.
PATRICi32116637. VBIEscCol129921_0751.

Organism-specific databases

EchoBASEiEB0926.
EcoGeneiEG10933. sdhC.

Phylogenomic databases

eggNOGiENOG4108UTY. Bacteria.
COG2009. LUCA.
HOGENOMiHOG000160252.
InParanoidiP69054.
KOiK00241.
OMAiIVKFIVW.
PhylomeDBiP69054.

Enzyme and pathway databases

UniPathwayiUPA00223.
BioCyciEcoCyc:SDH-MEMB1.
ECOL316407:JW0711-MONOMER.
MetaCyc:SDH-MEMB1.

Miscellaneous databases

EvolutionaryTraceiP69054.
PROiP69054.

Family and domain databases

InterProiIPR018495. Succ_DH_cyt_bsu_CS.
IPR014314. Succ_DH_cytb556.
IPR000701. Succ_DH_Fumarate_Rdtase_TM-su.
[Graphical view]
PfamiPF01127. Sdh_cyt. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02970. succ_dehyd_cytB. 1 hit.
PROSITEiPS01000. SDH_CYT_1. 1 hit.
PS01001. SDH_CYT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDHSC_ECOLI
AccessioniPrimary (citable) accession number: P69054
Secondary accession number(s): P10446
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: February 1, 2005
Last modified: September 7, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Miscellaneous

His-84 provides an axial ligand to heme b556 in the wild-type enzyme. The retention of low spin heme b556 in the SdhC 'Leu-84' mutant suggests that swapping of the heme axial ligand by His-91 or His-30 is possible.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.