P69040 (IF5A1_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Eukaryotic translation initiation factor 5A-1
Short name=eIF-5A-1

Alternative name(s):
eIF-4D

Gene names

Name:

EIF-5A1

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	159 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The precise role of eIF-5A in protein biosynthesis is not known but it functions by promoting the formation of the first peptide bond.
Post-translational modification	eIF-5A seems to be the only eukaryotic protein to have a hypusine residue which is a post-translational modification of a lysine by the addition of a butylamino group (from spermidine).
Miscellaneous	There are at least two genes for eIF-5A in tobacco: 5A1 may regulate the light-dependent translation of specific transcripts while 5A2 may be a housekeeping protein.
Sequence similarities	Belongs to the eIF-5A family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Molecular function	Initiation factor
PTM	Hypusine
Gene Ontology (GO)
Biological_process	peptidyl-lysine modification to hypusine Inferred from electronic annotation. Source: InterPro positive regulation of translational elongation Inferred from electronic annotation. Source: InterPro positive regulation of translational termination Inferred from electronic annotation. Source: InterPro translational frameshifting Inferred from electronic annotation. Source: InterPro
Molecular_function	ribosome binding Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: InterPro translation initiation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 159	159	Eukaryotic translation initiation factor 5A-1		PRO_0000142482
Amino acid modifications
	Modified residue	52	1	Hypusine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P69040 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: 5174BC5809E8E275	FASTA	159	17,371
	10 20 30 40 50 60 MSDEEHHFES KADAGASKTY PQQAGTIRKN GHIVIKNRPC KVVEVSTSKT GKHGHAKCHF 70 80 90 100 110 120 VAIDIFTGKK LEDIVPSSHN CDVPHVNRTD YQLIDISEDG FVSLLTENGN TKDDLRLPTD 130 140 150 DNLLALIKDG FAEGKDLVLS VMSAMGEEQI CGIKDVGPK

References

[1]	"Differential expression of genes encoding the hypusine-containing translation initiation factor, eIF-5A, in tobacco." Chamot D., Kuhlemeter C. Nucleic Acids Res. 20:665-669(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X63543 Genomic DNA. Translation: CAA45105.1.
PIR	S21060.
3D structure databases
ProteinModelPortal	P69040.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	2.30.30.30. 1 hit. 2.40.50.140. 1 hit.
InterPro	IPR005824. KOW. IPR012340. NA-bd_OB-fold. IPR014722. Rib_L2_dom2. IPR019769. Trans_elong_IF5A_hypusine_site. IPR001884. Transl_elong_IF5A. IPR020189. Transl_elong_IF5A_C. IPR008991. Translation_prot_SH3-like. [Graphical view]
PANTHER	PTHR11673. PTHR11673. 1 hit.
Pfam	PF01287. eIF-5a. 1 hit. PF00467. KOW. 1 hit. [Graphical view]
PIRSF	PIRSF003025. eIF5A. 1 hit.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF50104. Transl_SH3_like. 1 hit.
TIGRFAMs	TIGR00037. eIF_5A. 1 hit.
PROSITE	PS00302. IF5A_HYPUSINE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IF5A1_TOBAC

Accession

Primary (citable) accession number: P69040
Secondary accession number(s): P24921

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	February 1, 2005
Last modified:	April 3, 2013
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program