P69000 (AMPM_CLOAB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionine aminopeptidase
Short name=MAP
EC=3.4.11.18

Alternative name(s):
Peptidase M

Gene names

Name:	map
Ordered Locus Names:	CA_C3111

Organism

Clostridium acetobutylicum

Taxonomic identifier

1488 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Clostridiales › Clostridiaceae › Clostridium

Protein attributes

Sequence length	250 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Removes the amino-terminal methionine from nascent proteins.
Catalytic activity	Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.
Cofactor	Binds 2 cobalt ions per subunit. The true nature of the physiological cofactor is under debate. The enzyme is also active with zinc, manganese or divalent iron ions By similarity.
Sequence similarities	Belongs to the peptidase M24A family.

Ontologies

Keywords
Ligand	Cobalt Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW metalloexopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 250	250	Methionine aminopeptidase		PRO_0000148935
Sites
	Metal binding	95	1	Cobalt 1 By similarity
	Metal binding	106	1	Cobalt 1 By similarity
	Metal binding	106	1	Cobalt 2 By similarity
	Metal binding	169	1	Cobalt 2 By similarity
	Metal binding	202	1	Cobalt 2 By similarity
	Metal binding	233	1	Cobalt 1 By similarity
	Metal binding	233	1	Cobalt 2 By similarity
	Binding site	77	1	Substrate By similarity
	Binding site	176	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P69000 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: FE811B9E55B0584B	FASTA	250	27,301
	10 20 30 40 50 60 MIIIKNDTEI EYMRQAGKIV GETLNMLEKA AKPGVTTADL DRLAEDFIKK YNAIPSFKGY 70 80 90 100 110 120 GGFPASICTS INEEVIHGIP SKHRVLHEGD IISVDCGAIL NGYQGDAART FAIGEISEEA 130 140 150 160 170 180 AKLIKVTKES FFKGVEKAVI GNRLTDISHS IQEYVESFGY GVVRDYVGHG IGKEMHEDPE 190 200 210 220 230 240 VPNYGRPGRG PKLVHGMVLA IEPMVDVGTY MVKTQSNDWT VVTQDGSLAA HYENTVAILD 250 NGPEILTLCE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome sequence and comparative analysis of the solvent-producing bacterium Clostridium acetobutylicum." Noelling J., Breton G., Omelchenko M.V., Makarova K.S., Zeng Q., Gibson R., Lee H.M., Dubois J., Qiu D., Hitti J., Wolf Y.I., Tatusov R.L., Sabathe F., Doucette-Stamm L.A., Soucaille P., Daly M.J., Bennett G.N., Koonin E.V., Smith D.R. J. Bacteriol. 183:4823-4838(2001) [PubMed: 11466286] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787.
[2]	"Improving genome annotations using phylogenetic profile anomaly detection." Mikkelsen T.S., Galagan J.E., Mesirov J.P. Bioinformatics 21:464-470(2005) [PubMed: 15374867] [Abstract] Cited for: IDENTIFICATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001437 Genomic DNA. No translation available.
3D structure databases
ProteinModelPortal	P69000.
SMR	P69000. Positions 1-248.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GenomeReviews	Gene locus CA_C3111 in contig AE001437_GR.
NMPDR	fig|272562.1.peg.3884.
Phylogenomic databases
HOGENOM	HBG299384.
OMA	CEILTDR.
PhylomeDB	P69000.
Family and domain databases
InterPro	IPR001714. Pept_M24_MAP. IPR000994. Pept_M24_structural-domain. IPR002467. Pept_M24A_MAP1. [Graphical view]
Gene3D	G3DSA:3.90.230.10. Peptidase_M24_cat_core. 1 hit.
Pfam	PF00557. Peptidase_M24. 1 hit. [Graphical view]
PRINTS	PR00599. MAPEPTIDASE.
SUPFAM	SSF55920. Peptidase_M24_cat_core. 1 hit.
TIGRFAMs	TIGR00500. Met_pdase_I. 1 hit.
PROSITE	PS00680. MAP_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPM_CLOAB

Accession

Primary (citable) accession number: P69000

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	February 1, 2005
Last modified:	December 14, 2011
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program