P68979 (E3GL_ADE06) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Early E3 18.5 kDa glycoprotein Alternative name(s): E3-19K E3gp 19 kDa Short name=E19 GP19K
Organism	Human adenovirus C serotype 6 (HAdV-6) (Human adenovirus 6)
Taxonomic identifier	10534 [NCBI]
Taxonomic lineage	Viruses › dsDNA viruses, no RNA stage › Adenoviridae › Mastadenovirus › Human adenovirus C
Virus host	Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	159 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds and retains class I heavy chains in the endoplasmic reticulum during the early period of virus infection, thereby impairing their transport to the cell surface. Also delays the expression of class I alleles that it cannot affect by direct retention. Binds transporters associated with antigen processing (TAP) and acts as a tapasin inhibitor, preventing class I/TAP association. In consequence, infected cells are masked for immune recognition by cytotoxic T-lymphocytes By similarity.
Subcellular location	Host endoplasmic reticulum membrane; Single-pass type I membrane protein.
Developmental stage	Expressed at early period of virus infection.
Domain	The lumenal domain binds directly to the peptide-binding domain of class I molecules. The di-lysine motif confers endoplasmic reticulum localization for type I membrane proteins By similarity.
Post-translational modification	Both disulfide bonds are absolutely critical for the interaction with MHC antigens By similarity. N-glycosylated; high-mannose By similarity.
Sequence similarities	Belongs to the adenoviridae E19 family.

Ontologies

Keywords
Biological process	Host-virus interaction Inhibition of host adaptive immune response by virus Inhibition of host tapasin by virus Viral immunoevasion
Cellular component	Host endoplasmic reticulum Host membrane Membrane
Developmental stage	Early protein
Domain	Signal Transmembrane Transmembrane helix
Ligand	Lectin Mannose-binding
PTM	Disulfide bond Glycoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	suppression by virus of host tapasin activity Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	mannose binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 17

17

By similarity

Chain

18 – 159

142

Early E3 18.5 kDa glycoprotein

PRO_0000036485

Regions

Topological domain

18 – 123

106

Lumenal Potential

Transmembrane

124 – 144

21

Helical; Potential

Topological domain

145 – 159

15

Cytoplasmic Potential

Motif

156 – 159

4

Di-lysine motif By similarity

Amino acid modifications

Glycosylation

29

1

N-linked (GlcNAc...); by host Potential

Glycosylation

78

1

N-linked (GlcNAc...); by host Potential

Disulfide bond

28 ↔ 45

By similarity

Disulfide bond

39 ↔ 100

By similarity

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

159

Helix Strand Turn

Details...

Beta strand

27 – 35

9

Beta strand

38 – 46

9

Beta strand

48 – 56

9

Beta strand

59 – 69

11

Beta strand

76 – 83

8

Beta strand

86 – 93

8

Helix

96 – 103

8

Helix

105 – 109

5

Sequences

	Sequence		Length	Mass (Da)
	P68979 [UniParc]. Last modified July 21, 1986. Version 1. Checksum: ED2519547E18AEB0	FASTA	159	18,438
	10 20 30 40 50 60 MRYMILGLLA LAAVCSAAKK VEFKEPACNV TFKSEANECT TLIKCTTEHE KLIIRHKDKI 70 80 90 100 110 120 GKYAVYAIWQ PGDTNDYNVT VFQGENRKTF MYKFPFYEMC DITMYMSKQY KLWPPQKCLE 130 140 150 NTGTFCSTAL LITALALVCT LLYLKYKSRR SFIDEKKMP

References

[1]	"Sequence analysis of group C human adenoviruses type 1 and 6 for five genes of region E3." Reichmann H., Schaarschmidt E., Geisler B., Hausmann J., Ortmann D., Bauer U., Flunker G., Seidel W. Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Y16037 Genomic DNA. Translation: CAA75990.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4E5X	X-ray	1.95	G/H	18-117	[»]

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

InterPro

IPR006965. Adenovirus_Gp19K.
[Graphical view]

Pfam

PF04881. Adeno_GP19K. 1 hit.
[Graphical view]

ProDom

PD006739. Adeno_GP19K. 1 hit.
[Graphical view] [Entries sharing at least one domain]

ProtoNet

Search...

Entry information

Entry name

E3GL_ADE06

Accession

Primary (citable) accession number: P68979
Secondary accession number(s): P03251

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	July 21, 1986
Last modified:	January 9, 2013
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program