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Protein

Mediator of RNA polymerase II transcription subunit 28

Gene

Med28

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May be part of a complex containing NF2/merlin that participates in cellular signaling to the actin cytoskeleton downstream of tyrosine kinase signaling pathways (By similarity). Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding, Activator
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 28
Alternative name(s):
Mediator complex subunit 28
Gene namesi
Name:Med28
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi1305875. Med28.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001139831 – 178Mediator of RNA polymerase II transcription subunit 28Add BLAST178

Proteomic databases

PaxDbiP68943.

Expressioni

Gene expression databases

BgeeiENSRNOG00000003592.
GenevisibleiP68943. RN.

Interactioni

Subunit structurei

Forms a ternary complex with NF2/merlin and GRB2. Binds to actin (By similarity). Component of the Mediator complex, which is probably composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP.By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004912.

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili109 – 145Sequence analysisAdd BLAST37

Sequence similaritiesi

Belongs to the Mediator complex subunit 28 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IETV. Eukaryota.
ENOG4111HUA. LUCA.
GeneTreeiENSGT00390000006192.
HOGENOMiHOG000113540.
HOVERGENiHBG052448.
InParanoidiP68943.
KOiK15141.
OMAiMQHKKPA.
OrthoDBiEOG091G0R50.
PhylomeDBiP68943.
TreeFamiTF326988.

Family and domain databases

InterProiView protein in InterPro
IPR021640. Mediator_Med28.
PfamiView protein in Pfam
PF11594. Med28. 1 hit.

Sequencei

Sequence statusi: Complete.

P68943-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASLGGMFA GQPPGPPPPP PGLPGQASLL QAAPGAPRPS NSTLVDELES
60 70 80 90 100
SFEACFASLV SQDYVNGTDQ EEIRTGVDQC IQKFLDIARQ TECFFLQKRL
110 120 130 140 150
QLSVQKPDQV IKEDVSELRS ELQRKDALVQ KHLTKLRHWQ QVLEDINVQH
160 170
KKPADMPQGS LAYLEQASAN IPAPLKQT
Length:178
Mass (Da):19,524
Last modified:February 1, 2005 - v1
Checksum:i73057B935817FB0B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AABR03090012 Genomic DNA. No translation available.
RefSeqiNP_001100687.1. NM_001107217.1.
UniGeneiRn.34860.

Genome annotation databases

EnsembliENSRNOT00000004912; ENSRNOP00000004912; ENSRNOG00000003592.
GeneIDi305391.
KEGGirno:305391.

Similar proteinsi

Entry informationi

Entry nameiMED28_RAT
AccessioniPrimary (citable) accession number: P68943
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: February 1, 2005
Last modified: November 22, 2017
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families