P68921 (LYS_BPVT2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lysozyme
EC=3.2.1.17

Alternative name(s):
Endolysin
Lysis protein
Muramidase

Gene names

Name:

R

Organism

Enterobacteria phage VT2-Sa (Bacteriophage VT2-Sa) [Complete proteome]

Taxonomic identifier

97081 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Caudovirales › Podoviridae › unclassified Podoviridae › Enterobacteria phage 933W sensu lato

Virus host

Escherichia coli O157:H7 [TaxID: 83334]

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Essential for lysis of bacterial cell wall, by showing cell wall hydrolyzing activity By similarity.
Catalytic activity	Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
Sequence similarities	Belongs to the glycosyl hydrolase 24 family.

Ontologies

Keywords
Molecular function	Antimicrobial Bacteriolytic enzyme Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cytolysis Inferred from electronic annotation. Source: UniProtKB-KW defense response to bacterium Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	lysozyme activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 177	177	Lysozyme		PRO_0000218090
Sites
	Active site	35	1	Proton donor By similarity
	Active site	44	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	P68921 [UniParc]. Last modified February 1, 2005. Version 1. Checksum: E546FDCB1201F036	FASTA	177	19,607
	10 20 30 40 50 60 MSRKLRYGLS AAVLALIAAG ASAPEILDQF LDEKEGNHTT AYRDGAGIWT ICRGATRVDG 70 80 90 100 110 120 KPVIPGMKLS KEKCDRVNAI ERDKALAWVE KNIKVPLTEP QKAGIASFCP YNIGPGKCFP 130 140 150 160 170 STFYRRINAG DRKGACEAIR WWIKDGGRDC RIRSNNCYGQ VSRRDQESAL ACWGIDR

References

[1]

"Sequence analysis of Stx2-converting phage VT2-Sa shows a great divergence in early regulation and replication regions." Miyamoto H., Nakai W., Yajima N., Fujibayashi A., Higuchi T., Sato K., Matsushiro A. DNA Res. 6:235-240(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP000363 Genomic DNA. Translation: BAA84328.1.
RefSeq	NP_050544.1. NC_000902.1.
3D structure databases
ProteinModelPortal	P68921.
ModBase	Search...
Protein family/group databases
CAZy	GH24. Glycoside Hydrolase Family 24.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1262225.
Phylogenomic databases
ProtClustDB	CLSP2343233.
Family and domain databases
Gene3D	1.10.530.40. 1 hit.
InterPro	IPR002196. Glyco_hydro_24. IPR023346. Lysozyme-like_dom. IPR023347. Lysozyme_dom. [Graphical view]
Pfam	PF00959. Phage_lysozyme. 1 hit. [Graphical view]
SUPFAM	SSF53955. SSF53955. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LYS_BPVT2

Accession

Primary (citable) accession number: P68921
Secondary accession number(s): Q9T0Q1

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 2005
Last sequence update:	February 1, 2005
Last modified:	May 1, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program