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Protein

Fructose-bisphosphate aldolase

Gene

fba

Organism
Streptococcus pyogenes serotype M18 (strain MGAS8232)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3-phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis.By similarity

Catalytic activityi

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit. One is catalytic and the other provides a structural contribution.By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (pgi)
  3. ATP-dependent 6-phosphofructokinase (pfkA)
  4. Fructose-bisphosphate aldolase (fba)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei50Glyceraldehyde 3-phosphateBy similarity1
Active sitei85Proton donorBy similarity1
Metal bindingi86Zinc 1; catalyticBy similarity1
Metal bindingi106Zinc 2By similarity1
Metal bindingi136Zinc 2By similarity1
Metal bindingi178Zinc 1; catalyticBy similarity1
Binding sitei179Dihydroxyacetone phosphate; via amide nitrogenBy similarity1
Metal bindingi208Zinc 1; catalyticBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayiUPA00109; UER00183.

Names & Taxonomyi

Protein namesi
Recommended name:
Fructose-bisphosphate aldolase (EC:4.1.2.13)
Short name:
FBP aldolase
Short name:
FBPA
Alternative name(s):
Fructose-1,6-bisphosphate aldolase
Gene namesi
Name:fba
Ordered Locus Names:spyM18_1954
OrganismiStreptococcus pyogenes serotype M18 (strain MGAS8232)
Taxonomic identifieri186103 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001787502 – 293Fructose-bisphosphate aldolaseAdd BLAST292

Structurei

3D structure databases

ProteinModelPortaliP68906.
SMRiP68906.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni209 – 211Dihydroxyacetone phosphate bindingBy similarity3
Regioni230 – 233Dihydroxyacetone phosphate bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000227793.
KOiK01624.
OMAiELCKDCI.

Family and domain databases

CDDicd00947. TBP_aldolase_IIB. 1 hit.
Gene3Di3.20.20.70. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR000771. FBA_II.
IPR011289. Fruc_bis_ald_class-2.
PfamiView protein in Pfam
PF01116. F_bP_aldolase. 1 hit.
PIRSFiPIRSF001359. F_bP_aldolase_II. 1 hit.
TIGRFAMsiTIGR00167. cbbA. 1 hit.
TIGR01859. fruc_bis_ald_. 1 hit.
PROSITEiView protein in PROSITE
PS00602. ALDOLASE_CLASS_II_1. 1 hit.
PS00806. ALDOLASE_CLASS_II_2. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P68906-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIVSAEKFV QAARENGYAV GGFNTNNLEW TQAILRAAEA KQAPVLIQTS
60 70 80 90 100
MGAAKYMGGY KVCQSLITNL VESMGITVPV AIHLDHGHYE DALECIEVGY
110 120 130 140 150
TSIMFDGSHL PVEENLAKTA EVVKIAHAKG VSVEAEVGTI GGEEDGIIGK
160 170 180 190 200
GELAPIEDAK AMVETGIDFL AAGIGNIHGP YPENWEGLAL DHLEKLTAAV
210 220 230 240 250
PGFPIVLHGG SGIPDDQIKE AIRLGVAKVN VNTESQIAFS NATREFARNY
260 270 280 290
EANEAEYDGK KLFDPRKFLA PGMKAVQGAV EERIDVFGSA NKA
Length:293
Mass (Da):31,208
Last modified:January 23, 2007 - v2
Checksum:i8ECD3C3E41A79FD2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE009949 Genomic DNA. Translation: AAL98448.1.
RefSeqiWP_002982850.1. NC_003485.1.

Genome annotation databases

EnsemblBacteriaiAAL98448; AAL98448; spyM18_1954.
KEGGispm:spyM18_1954.

Similar proteinsi

Entry informationi

Entry nameiALF_STRP8
AccessioniPrimary (citable) accession number: P68906
Secondary accession number(s): P82486
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 4, 2005
Last sequence update: January 23, 2007
Last modified: June 7, 2017
This is version 72 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families