P68885 (SPEB_STRP8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Streptopain
EC=3.4.22.10

Alternative name(s):
Exotoxin type B
SPE B
Streptococcal cysteine proteinase
Streptococcus peptidase A
Short name=SPP

Gene names

Name:	speB
Ordered Locus Names:	spyM18_2099

Organism

Streptococcus pyogenes serotype M18 [Complete proteome] [HAMAP]

Taxonomic identifier

301451 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Important streptococcal virulence factor which cleaves human fibronectin and degrades vitronectin. Also cleaves human IL1B precursor to form biologically active IL1B. Can induce apoptosis in human monocytes and epithelial cells in vitro, and reduces phagocytic activity in monocytic cells. Thus, may play a role in bacterial colonization, invasion, and inhibition of wound healing.
Catalytic activity	Preferential cleavage with hydrophobic residues at P2, P1 and P1'.
Subunit structure	Homodimer By similarity.
Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase C10 family.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease Toxin
PTM	Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	By similarity
	Propeptide	28 – 145	118	By similarity		PRO_0000028509
	Chain	146 – 398	253	Streptopain		PRO_0000028510
Sites
	Active site	192	1	Nucleophile By similarity
	Active site	340	1	Proton acceptor By similarity
Amino acid modifications
	Modified residue	192	1	Cysteine methyl disulfide; in zymogen form By similarity

Sequences

	Sequence		Length	Mass (Da)
	P68885 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 16FF180D720AEE0F	FASTA	398	43,174
	10 20 30 40 50 60 MNKKKLGIRL LSLLALGGFV LANPVFADQN FARNEKEAKD SAITFIQKSA AIKAGARSAE 70 80 90 100 110 120 DIKLDKVNLG GELSGSNMYV YNISTGGFVI VSGDKRSPEI LGYSTSGSFD ANGKENIASF 130 140 150 160 170 180 MESYVEQIKE NKKLDTTYAG TAEIKQPVVK SLLDSKGIHY NQGNPYNLLT PVIEKVKPGE 190 200 210 220 230 240 QSFVGQHAAT GCVATATAQI MKYHNYPNKG LKDYTYTLSS NNPYFNHPKN LFAAISTRQY 250 260 270 280 290 300 NWNNILPTYS GRESNVQKMA ISELMADVGI SVDMDYGPSS GSAGSSRVQR ALKENFGYNQ 310 320 330 340 350 360 SVHQINRSDF SKQDWEAQID KELSQNQPVY YQGVGKVGGH AFVIDGADGR NFYHVNWGWG 370 380 390 GVSDGFFRLD ALNPSALGTG GGAGGFNGYQ SAVVGIKP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A conserved Streptococcus pyogenes extracellular cysteine protease cleaves human fibronectin and degrades vitronectin." Kapur V., Topouzis S., Majesky M.W., Li L.L., Hamrick M.R., Hamill R.J., Patti J.M., Musser J.M. Microb. Pathog. 15:327-346(1993) [PubMed: 7516997] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: 156 / Serotype M18 and 300 / Serotype M18.
[2]	"Genome sequence and comparative microarray analysis of serotype M18 group A Streptococcus strains associated with acute rheumatic fever outbreaks." Smoot J.C., Barbian K.D., Van Gompel J.J., Smoot L.M., Chaussee M.S., Sylva G.L., Sturdevant D.E., Ricklefs S.M., Porcella S.F., Parkins L.D., Beres S.B., Campbell D.S., Smith T.M., Zhang Q., Kapur V., Daly J.A., Veasy L.G., Musser J.M. Proc. Natl. Acad. Sci. U.S.A. 99:4668-4673(2002) [PubMed: 11917108] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MGAS8232 / Serotype M18.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L26125 Genomic DNA. Translation: AAA26979.1. AE009949 Genomic DNA. Translation: AAL98559.1.
RefSeq	NP_608060.1. NC_003485.1.
3D structure databases
ProteinModelPortal	P68885.
SMR	P68885. Positions 31-398.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000029711; EBSTRP00000028633; EBSTRG00000029711.
GeneID	995105.
GenomeReviews	Gene locus spyM18_2099 in contig AE009949_GR.
KEGG	spm:spyM18_2099.
PATRIC	19750614. VBIStrPyo4396_1872.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000030149.
OMA	WESQIDK.
ProtClustDB	CLSK698945.
Enzyme and pathway databases
BioCyc	SPYO186103:SPYM18_2099-MONOMER.
Family and domain databases
InterPro	IPR000200. Peptidase_C10. [Graphical view]
KO	K01364.
Pfam	PF01640. Peptidase_C10. 1 hit. [Graphical view]
PRINTS	PR00797. STREPTOPAIN.
ProDom	PD004169. Peptidase_C10. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

SPEB_STRP8

Accession

Primary (citable) accession number: P68885
Secondary accession number(s): P00788 , P26296, Q54960, Q54961, Q54962, Q54963, Q54964, Q54965, Q54966, Q54967, Q54968, Q57024, Q57082, Q57202, Q57211, Q57212, Q9S680

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 4, 2005
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program