Reviewed, UniProtKB/Swiss-Prot P68884 (SPEB_STRP3)

Names and origin

Protein names

Recommended name:
    Streptopain
    EC=3.4.22.10
Alternative name(s):
    Streptococcal cysteine proteinase
    Streptococcus peptidase A
      Short name=SPP
    Exotoxin type B
    SPE B

Gene names

Name:	speB
Ordered Locus Names:	SpyM3_1742, SPs1739

Organism

Streptococcus pyogenes serotype M3 [Complete proteome] [HAMAP]

Taxonomic identifier

301448 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	398 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Important streptococcal virulence factor which cleaves human fibronectin and degrades vitronectin. Also cleaves human IL1B precursor to form biologically active IL1B. Can induce apoptosis in human monocytes and epithelial cells in vitro, and reduces phagocytic activity in monocytic cells. Thus, may play a role in bacterial colonization, invasion, and inhibition of wound healing.
Catalytic activity	Preferential cleavage with hydrophobic residues at P2, P1 and P1'.
Subcellular location	Secreted.
Sequence similarities	Belongs to the peptidase C10 family.

Ontologies

Keywords
Biological process	Virulence
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease Toxin
PTM	Zymogen
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	By similarity
	Propeptide	28 – 145	118	By similarity		PRO_0000028505
	Chain	146 – 398	253	Streptopain		PRO_0000028506
Sites
	Active site	192	1	By similarity
	Active site	340	1	By similarity
Amino acid modifications
	Modified residue	192	1	Cysteine methyl disulfide; in zymogen form By similarity

Sequences

	Sequence		Length	Mass (Da)
	P68884-1 [UniParc]. Last modified January 4, 2005. Version 1. Checksum: 16FF180D720AEE0F	FASTA	398	43,174
	10 20 30 40 50 60 MNKKKLGIRL LSLLALGGFV LANPVFADQN FARNEKEAKD SAITFIQKSA AIKAGARSAE 70 80 90 100 110 120 DIKLDKVNLG GELSGSNMYV YNISTGGFVI VSGDKRSPEI LGYSTSGSFD ANGKENIASF 130 140 150 160 170 180 MESYVEQIKE NKKLDTTYAG TAEIKQPVVK SLLDSKGIHY NQGNPYNLLT PVIEKVKPGE 190 200 210 220 230 240 QSFVGQHAAT GCVATATAQI MKYHNYPNKG LKDYTYTLSS NNPYFNHPKN LFAAISTRQY 250 260 270 280 290 300 NWNNILPTYS GRESNVQKMA ISELMADVGI SVDMDYGPSS GSAGSSRVQR ALKENFGYNQ 310 320 330 340 350 360 SVHQINRSDF SKQDWEAQID KELSQNQPVY YQGVGKVGGH AFVIDGADGR NFYHVNWGWG 370 380 390 GVSDGFFRLD ALNPSALGTG GGAGGFNGYQ SAVVGIKP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A conserved Streptococcus pyogenes extracellular cysteine protease cleaves human fibronectin and degrades vitronectin." Kapur V., Topouzis S., Majesky M.W., Li L.L., Hamrick M.R., Hamill R.J., Patti J.M., Musser J.M. Microb. Pathog. 15:327-346(1993) [PubMed: 7516997] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: 1251 / Serotype M3, 157 / Serotype M3, 75 / Serotype M3 and ATCC BAA-595 / MGAS315 / Serotype M3.
[2]	"Genome sequence of a serotype M3 strain of group A Streptococcus: phage-encoded toxins, the high-virulence phenotype, and clone emergence." Beres S.B., Sylva G.L., Barbian K.D., Lei B., Hoff J.S., Mammarella N.D., Liu M.-Y., Smoot J.C., Porcella S.F., Parkins L.D., Campbell D.S., Smith T.M., McCormick J.K., Leung D.Y.M., Schlievert P.M., Musser J.M. Proc. Natl. Acad. Sci. U.S.A. 99:10078-10083(2002) [PubMed: 12122206] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC BAA-595 / MGAS315 / Serotype M3.
[3]	"Genome sequence of an M3 strain of Streptococcus pyogenes reveals a large-scale genomic rearrangement in invasive strains and new insights into phage evolution." Nakagawa I., Kurokawa K., Yamashita A., Nakata M., Tomiyasu Y., Okahashi N., Kawabata S., Yamazaki K., Shiba T., Yasunaga T., Hayashi H., Hattori M., Hamada S. Genome Res. 13:1042-1055(2003) [PubMed: 12799345] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SSI-1 / Serotype M3.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L26146 Genomic DNA. Translation: AAA27012.1. AE014074 Genomic DNA. Translation: AAM80349.1. BA000034 Genomic DNA. Translation: BAC64834.1.
RefSeq	NP_665546.1. NP_803001.1.
3D structure databases
SMR	P68884. Positions 31-398.
ModBase	Search...
Genome annotation databases
GeneID	1010057. 1066249.
GenomeReviews	Gene locus SpyM3_1742 in contig AE014074_GR. Gene locus SPs1739 in contig BA000034_GR.
KEGG	spg:SpyM3_1742. sps:SPs1739.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	P68884. WESQIDK.
Enzyme and pathway databases
BioCyc	SPYO193567:SPS1739-MON. SPYO198466:SPYM3_1742-MON.
Family and domain databases
InterPro	IPR000200. Peptidase_C10. [Graphical view]
Pfam	PF01640. Peptidase_C10. 1 hit. [Graphical view]
PRINTS	PR00797. STREPTOPAIN.
ProDom	PD004169. Peptidase_C10. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

SPEB_STRP3

Accession

Primary (citable) accession number: P68884
Secondary accession number(s): P00788 , P26296, Q54960, Q54961, Q54962, Q54963, Q54964, Q54965, Q54966, Q54967, Q54968, Q57024, Q57082, Q57202, Q57211, Q57212, Q9S680

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 4, 2005
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)