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P68831

- B2_FHV

UniProt

P68831 - B2_FHV

Protein

Protein B2

Gene

B2

Organism
Flock house virus (FHV)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
  1. Functioni

    Binds double-strand RNA (dsRNA) with high affinity. Suppresses the host RNA silencing-based antiviral response.2 Publications

    GO - Molecular functioni

    1. RNA binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Suppressor of RNA silencing

    Keywords - Ligandi

    RNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein B2
    Gene namesi
    Name:B2
    OrganismiFlock house virus (FHV)
    Taxonomic identifieri12287 [NCBI]
    Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNodaviridaeAlphanodavirus
    Virus hostiCostelytra zealandica [TaxID: 50579]
    Galleria mellonella (Greater wax moth) [TaxID: 7137]
    Hordeum vulgare (Barley) [TaxID: 4513]
    Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 106106Protein B2PRO_0000222454Add
    BLAST

    Expressioni

    Developmental stagei

    Expressed at high levels early in the viral replication cycle.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    DIPiDIP-60497N.

    Structurei

    Secondary structure

    1
    106
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 96
    Helixi11 – 2010
    Helixi22 – 243
    Beta strandi28 – 303
    Helixi32 – 5928
    Helixi63 – 708

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2AZ0X-ray2.60A/B1-73[»]
    2AZ2X-ray2.60A/B1-73[»]
    2B9ZNMR-A/B1-72[»]
    ProteinModelPortaliP68831.
    SMRiP68831. Positions 2-72.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP68831.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 7373RNA-bindingAdd
    BLAST

    Domaini

    The dsRNA-binding region is required for suppression of RNA silencing.

    Sequence similaritiesi

    Belongs to the nodaviridae B2 protein family.Curated

    Family and domain databases

    InterProiIPR024377. RNA-bd_B2.
    [Graphical view]
    PfamiPF11473. B2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P68831-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPSKLALIQE LPDRIQTAVE AAMGMSYQDA PNNVRRDLDN LHACLNKAKL    50
    TVSRMVTSLL EKPSVVAYLE GKAPEEAKPT LEERLRKLEL SHSLPTTGSD 100
    PPPAKL 106
    Length:106
    Mass (Da):11,646
    Last modified:December 21, 2004 - v1
    Checksum:iFA79D28C4AB99CA9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X77156 Genomic RNA. Translation: CAA54401.1.
    PIRiS41398.
    RefSeqiNP_689446.1. NC_004146.1.

    Genome annotation databases

    GeneIDi1724847.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X77156 Genomic RNA. Translation: CAA54401.1 .
    PIRi S41398.
    RefSeqi NP_689446.1. NC_004146.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2AZ0 X-ray 2.60 A/B 1-73 [» ]
    2AZ2 X-ray 2.60 A/B 1-73 [» ]
    2B9Z NMR - A/B 1-72 [» ]
    ProteinModelPortali P68831.
    SMRi P68831. Positions 2-72.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60497N.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 1724847.

    Miscellaneous databases

    EvolutionaryTracei P68831.

    Family and domain databases

    InterProi IPR024377. RNA-bd_B2.
    [Graphical view ]
    Pfami PF11473. B2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Near identity in the polymerase gene of two serologically distinct nodaviruses."
      Dasgupta R.
      Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
    2. "Interferon antagonist proteins of influenza and vaccinia viruses are suppressors of RNA silencing."
      Li W.-X., Li H., Lu R., Li F., Dus M., Atkinson P., Brydon E.W.A., Johnson K.L., Garcia-Sastre A., Ball L.A., Palese P., Ding S.-W.
      Proc. Natl. Acad. Sci. U.S.A. 101:1350-1355(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    3. "Dual modes of RNA-silencing suppression by Flock House virus protein B2."
      Chao J.A., Lee J.H., Chapados B.R., Debler E.W., Schneemann A., Williamson J.R.
      Nat. Struct. Mol. Biol. 12:952-957(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, RNA-BINDING.

    Entry informationi

    Entry nameiB2_FHV
    AccessioniPrimary (citable) accession number: P68831
    Secondary accession number(s): P29100, Q89618, Q96632
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 21, 2004
    Last sequence update: December 21, 2004
    Last modified: October 1, 2014
    This is version 25 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Encoded on a subgenomic RNA (RNA3) synthesized during replication and which is co-terminal with RNA1.

    Keywords - Technical termi

    3D-structure

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3