P68831 (B2_FHV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein B2

Gene names

Name:

B2

Organism

Flock house virus (FHV)

Taxonomic identifier

12287 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA positive-strand viruses, no DNA stage › Nodaviridae › Alphanodavirus

Virus host

Costelytra zealandica [TaxID: 50579]
Galleria mellonella (Greater wax moth) [TaxID: 7137]
Hordeum vulgare (Barley) [TaxID: 4513]
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932]

Protein attributes

Sequence length	106 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds double-strand RNA (dsRNA) with high affinity. Suppresses the host RNA silencing-based antiviral response. Ref.2 Ref.3
Subunit structure	Homodimer. Ref.3
Developmental stage	Expressed at high levels early in the viral replication cycle.
Domain	The dsRNA-binding region is required for suppression of RNA silencing.
Miscellaneous	Encoded on a subgenomic RNA (RNA3) synthesized during replication and which is co-terminal with RNA1.
Sequence similarities	Belongs to the nodaviridae B2 protein family.

Ontologies

Keywords
Ligand	RNA-binding
Molecular function	Suppressor of RNA silencing
Technical term	3D-structure
Gene Ontology (GO)
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 106

106

Protein B2

PRO_0000222454

Regions

Region

1 – 73

73

RNA-binding

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

106

Helix Strand Turn

Details...

Helix

4 – 9

6

Helix

11 – 20

10

Helix

22 – 24

3

Beta strand

28 – 30

3

Helix

32 – 59

28

Helix

63 – 70

8

Sequences

	Sequence		Length	Mass (Da)
	P68831 [UniParc]. Last modified December 21, 2004. Version 1. Checksum: FA79D28C4AB99CA9	FASTA	106	11,646
	10 20 30 40 50 60 MPSKLALIQE LPDRIQTAVE AAMGMSYQDA PNNVRRDLDN LHACLNKAKL TVSRMVTSLL 70 80 90 100 EKPSVVAYLE GKAPEEAKPT LEERLRKLEL SHSLPTTGSD PPPAKL

References

[1]	"Near identity in the polymerase gene of two serologically distinct nodaviruses." Dasgupta R. Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Interferon antagonist proteins of influenza and vaccinia viruses are suppressors of RNA silencing." Li W.-X., Li H., Lu R., Li F., Dus M., Atkinson P., Brydon E.W.A., Johnson K.L., Garcia-Sastre A., Ball L.A., Palese P., Ding S.-W. Proc. Natl. Acad. Sci. U.S.A. 101:1350-1355(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[3]	"Dual modes of RNA-silencing suppression by Flock House virus protein B2." Chao J.A., Lee J.H., Chapados B.R., Debler E.W., Schneemann A., Williamson J.R. Nat. Struct. Mol. Biol. 12:952-957(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBUNIT, RNA-BINDING.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X77156 Genomic RNA. Translation: CAA54401.1.

PIR

S41398.

RefSeq

NP_689446.1. NC_004146.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2AZ0	X-ray	2.60	A/B	1-73	[»]
2AZ2	X-ray	2.60	A/B	1-73	[»]
2B9Z	NMR	-	A/B	1-72	[»]

ProteinModelPortal

P68831.

SMR

P68831. Positions 2-72.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

1724847.

Family and domain databases

InterPro

IPR024377. RNA-bd_B2.
[Graphical view]

Pfam

PF11473. B2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P68831.

Entry information

Entry name

B2_FHV

Accession

Primary (citable) accession number: P68831
Secondary accession number(s): P29100, Q89618, Q96632

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 21, 2004
Last sequence update:	December 21, 2004
Last modified:	April 18, 2012
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program