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Protein

Protein B2

Gene

B2

Organism
Flock house virus (FHV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Binds double-strand RNA (dsRNA) with high affinity. Suppresses the host RNA silencing-based antiviral response.2 Publications

GO - Molecular functioni

  1. RNA binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Suppressor of RNA silencing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein B2
Gene namesi
Name:B2
OrganismiFlock house virus (FHV)
Taxonomic identifieri12287 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stageNodaviridaeAlphanodavirus
Virus hostiCostelytra zealandica [TaxID: 50579]
Galleria mellonella (Greater wax moth) [TaxID: 7137]
Hordeum vulgare (Barley) [TaxID: 4513]
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 106106Protein B2PRO_0000222454Add
BLAST

Expressioni

Developmental stagei

Expressed at high levels early in the viral replication cycle.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

DIPiDIP-60497N.

Structurei

Secondary structure

1
106
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 96Combined sources
Helixi11 – 2010Combined sources
Helixi22 – 243Combined sources
Beta strandi28 – 303Combined sources
Helixi32 – 5928Combined sources
Helixi63 – 708Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AZ0X-ray2.60A/B1-73[»]
2AZ2X-ray2.60A/B1-73[»]
2B9ZNMR-A/B1-72[»]
ProteinModelPortaliP68831.
SMRiP68831. Positions 2-72.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP68831.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 7373RNA-bindingAdd
BLAST

Domaini

The dsRNA-binding region is required for suppression of RNA silencing.

Sequence similaritiesi

Belongs to the nodaviridae B2 protein family.Curated

Family and domain databases

InterProiIPR024377. RNA-bd_B2.
[Graphical view]
PfamiPF11473. B2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P68831-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPSKLALIQE LPDRIQTAVE AAMGMSYQDA PNNVRRDLDN LHACLNKAKL
60 70 80 90 100
TVSRMVTSLL EKPSVVAYLE GKAPEEAKPT LEERLRKLEL SHSLPTTGSD

PPPAKL
Length:106
Mass (Da):11,646
Last modified:December 21, 2004 - v1
Checksum:iFA79D28C4AB99CA9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77156 Genomic RNA. Translation: CAA54401.1.
PIRiS41398.
RefSeqiNP_689446.1. NC_004146.1.

Genome annotation databases

GeneIDi1724847.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77156 Genomic RNA. Translation: CAA54401.1.
PIRiS41398.
RefSeqiNP_689446.1. NC_004146.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2AZ0X-ray2.60A/B1-73[»]
2AZ2X-ray2.60A/B1-73[»]
2B9ZNMR-A/B1-72[»]
ProteinModelPortaliP68831.
SMRiP68831. Positions 2-72.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-60497N.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1724847.

Miscellaneous databases

EvolutionaryTraceiP68831.

Family and domain databases

InterProiIPR024377. RNA-bd_B2.
[Graphical view]
PfamiPF11473. B2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Near identity in the polymerase gene of two serologically distinct nodaviruses."
    Dasgupta R.
    Submitted (JAN-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Interferon antagonist proteins of influenza and vaccinia viruses are suppressors of RNA silencing."
    Li W.-X., Li H., Lu R., Li F., Dus M., Atkinson P., Brydon E.W.A., Johnson K.L., Garcia-Sastre A., Ball L.A., Palese P., Ding S.-W.
    Proc. Natl. Acad. Sci. U.S.A. 101:1350-1355(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  3. "Dual modes of RNA-silencing suppression by Flock House virus protein B2."
    Chao J.A., Lee J.H., Chapados B.R., Debler E.W., Schneemann A., Williamson J.R.
    Nat. Struct. Mol. Biol. 12:952-957(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT, RNA-BINDING.

Entry informationi

Entry nameiB2_FHV
AccessioniPrimary (citable) accession number: P68831
Secondary accession number(s): P29100, Q89618, Q96632
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: December 21, 2004
Last modified: November 26, 2014
This is version 26 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Miscellaneous

Encoded on a subgenomic RNA (RNA3) synthesized during replication and which is co-terminal with RNA1.

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.