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Protein

Elongation factor G

Gene

fusA

Organism
Staphylococcus aureus
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi17 – 248GTPBy similarity
Nucleotide bindingi81 – 855GTPBy similarity
Nucleotide bindingi135 – 1384GTPBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Elongation factor

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Elongation factor G
Short name:
EF-G
Alternative name(s):
85 kDa vitronectin-binding protein
Gene namesi
Name:fusA
Synonyms:fus
OrganismiStaphylococcus aureus
Taxonomic identifieri1280 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 693692Elongation factor GPRO_0000091219Add
BLAST

Proteomic databases

PRIDEiP68790.

Interactioni

Subunit structurei

Has vitronectin-binding activity.

Protein-protein interaction databases

STRINGi93062.SACOL0593.

Structurei

Secondary structure

1
693
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 93Combined sources
Beta strandi10 – 167Combined sources
Helixi19 – 213Combined sources
Helixi23 – 3412Combined sources
Helixi47 – 504Combined sources
Turni51 – 533Combined sources
Beta strandi66 – 727Combined sources
Beta strandi75 – 806Combined sources
Helixi90 – 989Combined sources
Beta strandi100 – 1078Combined sources
Turni108 – 1103Combined sources
Helixi114 – 12512Combined sources
Beta strandi130 – 1356Combined sources
Helixi144 – 15512Combined sources
Beta strandi159 – 1668Combined sources
Helixi169 – 1713Combined sources
Beta strandi174 – 1774Combined sources
Turni178 – 1814Combined sources
Beta strandi182 – 1854Combined sources
Beta strandi188 – 1914Combined sources
Beta strandi195 – 1973Combined sources
Helixi201 – 2033Combined sources
Helixi204 – 21916Combined sources
Helixi223 – 2308Combined sources
Helixi237 – 24913Combined sources
Beta strandi254 – 2585Combined sources
Turni261 – 2644Combined sources
Helixi267 – 27711Combined sources
Helixi281 – 2833Combined sources
Beta strandi287 – 2915Combined sources
Beta strandi294 – 3018Combined sources
Beta strandi305 – 3073Combined sources
Beta strandi310 – 31910Combined sources
Turni320 – 3223Combined sources
Beta strandi323 – 33614Combined sources
Beta strandi339 – 3435Combined sources
Turni344 – 3474Combined sources
Beta strandi348 – 3525Combined sources
Beta strandi355 – 3584Combined sources
Beta strandi363 – 3653Combined sources
Beta strandi367 – 3704Combined sources
Beta strandi374 – 3796Combined sources
Beta strandi388 – 3914Combined sources
Beta strandi407 – 41610Combined sources
Helixi417 – 43317Combined sources
Beta strandi438 – 4403Combined sources
Beta strandi448 – 4547Combined sources
Helixi455 – 46814Combined sources
Beta strandi473 – 4753Combined sources
Beta strandi483 – 4886Combined sources
Beta strandi490 – 4989Combined sources
Beta strandi505 – 51511Combined sources
Beta strandi523 – 5264Combined sources
Beta strandi530 – 5334Combined sources
Helixi535 – 5373Combined sources
Helixi538 – 55013Combined sources
Beta strandi553 – 5564Combined sources
Beta strandi561 – 57010Combined sources
Turni573 – 5753Combined sources
Helixi578 – 59316Combined sources
Beta strandi598 – 61215Combined sources
Helixi613 – 6153Combined sources
Helixi616 – 62510Combined sources
Beta strandi629 – 6368Combined sources
Beta strandi639 – 6479Combined sources
Helixi648 – 6503Combined sources
Helixi654 – 6618Combined sources
Turni662 – 6643Combined sources
Beta strandi667 – 67711Combined sources
Helixi680 – 69011Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2XEXX-ray1.90A/B1-693[»]
3ZZ0X-ray2.80A/B1-693[»]
3ZZTX-ray2.95A/B1-693[»]
3ZZUX-ray2.98A/B1-693[»]
ProteinModelPortaliP68790.
SMRiP68790. Positions 2-692.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP68790.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 282275tr-type GAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CEJ. Bacteria.
COG0480. LUCA.

Family and domain databases

CDDicd01434. EFG_mtEFG1_IV. 1 hit.
Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00054_B. EF_G_EF_2_B. 1 hit.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004540. Transl_elong_EFG/EF2.
IPR005517. Transl_elong_EFG/EF2_IV.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00484. EF-G. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P68790-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAREFSLEKT RNIGIMAHID AGKTTTTERI LYYTGRIHKI GETHEGASQM
60 70 80 90 100
DWMEQEQDRG ITITSAATTA AWEGHRVNII DTPGHVDFTV EVERSLRVLD
110 120 130 140 150
GAVTVLDAQS GVEPQTETVW RQATTYGVPR IVFVNKMDKL GANFEYSVST
160 170 180 190 200
LHDRLQANAA PIQLPIGAED EFEAIIDLVE MKCFKYTNDL GTEIEEIEIP
210 220 230 240 250
EDHLDRAEEA RASLIEAVAE TSDELMEKYL GDEEISVSEL KEAIRQATTN
260 270 280 290 300
VEFYPVLCGT AFKNKGVQLM LDAVIDYLPS PLDVKPIIGH RASNPEEEVI
310 320 330 340 350
AKADDSAEFA ALAFKVMTDP YVGKLTFFRV YSGTMTSGSY VKNSTKGKRE
360 370 380 390 400
RVGRLLQMHA NSRQEIDTVY SGDIAAAVGL KDTGTGDTLC GEKNDIILES
410 420 430 440 450
MEFPEPVIHL SVEPKSKADQ DKMTQALVKL QEEDPTFHAH TDEETGQVII
460 470 480 490 500
GGMGELHLDI LVDRMKKEFN VECNVGAPMV SYRETFKSSA QVQGKFSRQS
510 520 530 540 550
GGRGQYGDVH IEFTPNETGA GFEFENAIVG GVVPREYIPS VEAGLKDAME
560 570 580 590 600
NGVLAGYPLI DVKAKLYDGS YHDVDSSEMA FKIAASLALK EAAKKCDPVI
610 620 630 640 650
LEPMMKVTIE MPEEYMGDIM GDVTSRRGRV DGMEPRGNAQ VVNAYVPLSE
660 670 680 690
MFGYATSLRS NTQGRGTYTM YFDHYAEVPK SIAEDIIKKN KGE
Length:693
Mass (Da):76,612
Last modified:January 23, 2007 - v2
Checksum:iBAE273E3A33EEED9
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti4 – 41E → Q AA sequence (Ref. 2) Curated
Sequence conflicti8 – 81E → Q AA sequence (Ref. 2) Curated
Sequence conflicti19 – 191Missing AA sequence (Ref. 2) Curated
Sequence conflicti25 – 251T → L AA sequence (Ref. 2) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ237696 Genomic DNA. Translation: CAB40191.1.
RefSeqiWP_000090315.1. NZ_LT009690.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ237696 Genomic DNA. Translation: CAB40191.1.
RefSeqiWP_000090315.1. NZ_LT009690.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2XEXX-ray1.90A/B1-693[»]
3ZZ0X-ray2.80A/B1-693[»]
3ZZTX-ray2.95A/B1-693[»]
3ZZUX-ray2.98A/B1-693[»]
ProteinModelPortaliP68790.
SMRiP68790. Positions 2-692.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi93062.SACOL0593.

Proteomic databases

PRIDEiP68790.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105CEJ. Bacteria.
COG0480. LUCA.

Miscellaneous databases

EvolutionaryTraceiP68790.

Family and domain databases

CDDicd01434. EFG_mtEFG1_IV. 1 hit.
Gene3Di3.30.230.10. 1 hit.
3.30.70.240. 1 hit.
3.40.50.300. 1 hit.
HAMAPiMF_00054_B. EF_G_EF_2_B. 1 hit.
InterProiIPR009022. EFG_III-V.
IPR000640. EFG_V.
IPR004161. EFTu-like_2.
IPR031157. G_TR_CS.
IPR027417. P-loop_NTPase.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR014721. Ribosomal_S5_D2-typ_fold_subgr.
IPR005225. Small_GTP-bd_dom.
IPR000795. TF_GTP-bd_dom.
IPR009000. Transl_B-barrel.
IPR004540. Transl_elong_EFG/EF2.
IPR005517. Transl_elong_EFG/EF2_IV.
[Graphical view]
PfamiPF00679. EFG_C. 1 hit.
PF14492. EFG_II. 1 hit.
PF03764. EFG_IV. 1 hit.
PF03144. GTP_EFTU_D2. 1 hit.
[Graphical view]
PRINTSiPR00315. ELONGATNFCT.
SMARTiSM00838. EFG_C. 1 hit.
SM00889. EFG_IV. 1 hit.
[Graphical view]
SUPFAMiSSF50447. SSF50447. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF54211. SSF54211. 1 hit.
SSF54980. SSF54980. 2 hits.
TIGRFAMsiTIGR00484. EF-G. 1 hit.
TIGR00231. small_GTP. 1 hit.
PROSITEiPS00301. G_TR_1. 1 hit.
PS51722. G_TR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEFG_STAAU
AccessioniPrimary (citable) accession number: P68790
Secondary accession number(s): P81683, Q9X7M3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.