Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P68771

- DEF_STRP1

UniProt

P68771 - DEF_STRP1

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Peptide deformylase

Gene

def

Organism
Streptococcus pyogenes serotype M1
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.UniRule annotation

Catalytic activityi

Formyl-L-methionyl peptide + H2O = formate + methionyl peptide.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe(2+) ion.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi131 – 1311IronUniRule annotation
Metal bindingi174 – 1741IronUniRule annotation
Active sitei175 – 1751UniRule annotation
Metal bindingi178 – 1781IronUniRule annotation

GO - Molecular functioni

  1. iron ion binding Source: InterPro
  2. peptide deformylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. translation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciSPYO160490:GJ81-1613-MONOMER.
SPYO293653:GHFC-1748-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptide deformylaseUniRule annotation (EC:3.5.1.88UniRule annotation)
Short name:
PDFUniRule annotation
Alternative name(s):
Polypeptide deformylaseUniRule annotation
Gene namesi
Name:defUniRule annotation
Ordered Locus Names:SPy_1958, M5005_Spy1669
OrganismiStreptococcus pyogenes serotype M1
Taxonomic identifieri301447 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesStreptococcaceaeStreptococcus
ProteomesiUP000000750: Chromosome, UP000002702: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 204204Peptide deformylasePRO_0000082859Add
BLAST

Proteomic databases

PaxDbiP68771.

Interactioni

Protein-protein interaction databases

STRINGi160490.SPy_1958.

Structurei

Secondary structure

1
204
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 75Combined sources
Helixi16 – 183Combined sources
Helixi25 – 284Combined sources
Helixi40 – 5617Combined sources
Helixi59 – 657Combined sources
Beta strandi71 – 744Combined sources
Helixi75 – 784Combined sources
Beta strandi82 – 909Combined sources
Beta strandi101 – 11818Combined sources
Beta strandi120 – 1256Combined sources
Beta strandi144 – 15310Combined sources
Beta strandi159 – 1646Combined sources
Helixi166 – 17813Combined sources
Turni179 – 1813Combined sources
Helixi184 – 1874Combined sources
Beta strandi200 – 2034Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2OS3X-ray2.26A2-204[»]
ProteinModelPortaliP68771.
SMRiP68771. Positions 2-204.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP68771.

Family & Domainsi

Sequence similaritiesi

Belongs to the polypeptide deformylase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0242.
HOGENOMiHOG000243507.
KOiK01462.
OMAiHIDKENP.
OrthoDBiEOG6PZXGQ.

Family and domain databases

Gene3Di3.90.45.10. 1 hit.
HAMAPiMF_00163. Pep_deformylase.
InterProiIPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view]
PANTHERiPTHR10458. PTHR10458. 1 hit.
PfamiPF01327. Pep_deformylase. 1 hit.
[Graphical view]
PIRSFiPIRSF004749. Pep_def. 1 hit.
PRINTSiPR01576. PDEFORMYLASE.
SUPFAMiSSF56420. SSF56420. 1 hit.
TIGRFAMsiTIGR00079. pept_deformyl. 1 hit.

Sequencei

Sequence statusi: Complete.

P68771-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSAQDKLIKP SHLITMDDII REGNPTLRAV AKEVSLPLCD EDILLGEKMM
60 70 80 90 100
QFLKHSQDPV MAEKLGLRAG VGLAAPQIDV SKRIIAVLVP NLPDKEGNPP
110 120 130 140 150
KEAYSWQEVL YNPKIVSHSV QDAALSDGEG CLSVDRVVEG YVVRHARVTV
160 170 180 190 200
DYYDKEGQQH RIKLKGYNAI VVQHEIDHIN GVLFYDRINA KNPFETKEEL

LILD
Length:204
Mass (Da):22,862
Last modified:December 21, 2004 - v1
Checksum:i89F8EDE94D94DC05
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004092 Genomic DNA. Translation: AAK34651.1.
CP000017 Genomic DNA. Translation: AAZ52287.1.
RefSeqiNP_269930.1. NC_002737.1.
YP_283032.1. NC_007297.1.

Genome annotation databases

EnsemblBacteriaiAAK34651; AAK34651; SPy_1958.
AAZ52287; AAZ52287; M5005_Spy1669.
GeneIDi3571223.
901633.
KEGGispy:SPy_1958.
spz:M5005_Spy_1669.
PATRICi19717319. VBIStrPyo79812_1705.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE004092 Genomic DNA. Translation: AAK34651.1 .
CP000017 Genomic DNA. Translation: AAZ52287.1 .
RefSeqi NP_269930.1. NC_002737.1.
YP_283032.1. NC_007297.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2OS3 X-ray 2.26 A 2-204 [» ]
ProteinModelPortali P68771.
SMRi P68771. Positions 2-204.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 160490.SPy_1958.

Proteomic databases

PaxDbi P68771.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAK34651 ; AAK34651 ; SPy_1958 .
AAZ52287 ; AAZ52287 ; M5005_Spy1669 .
GeneIDi 3571223.
901633.
KEGGi spy:SPy_1958.
spz:M5005_Spy_1669.
PATRICi 19717319. VBIStrPyo79812_1705.

Phylogenomic databases

eggNOGi COG0242.
HOGENOMi HOG000243507.
KOi K01462.
OMAi HIDKENP.
OrthoDBi EOG6PZXGQ.

Enzyme and pathway databases

BioCyci SPYO160490:GJ81-1613-MONOMER.
SPYO293653:GHFC-1748-MONOMER.

Miscellaneous databases

EvolutionaryTracei P68771.

Family and domain databases

Gene3Di 3.90.45.10. 1 hit.
HAMAPi MF_00163. Pep_deformylase.
InterProi IPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view ]
PANTHERi PTHR10458. PTHR10458. 1 hit.
Pfami PF01327. Pep_deformylase. 1 hit.
[Graphical view ]
PIRSFi PIRSF004749. Pep_def. 1 hit.
PRINTSi PR01576. PDEFORMYLASE.
SUPFAMi SSF56420. SSF56420. 1 hit.
TIGRFAMsi TIGR00079. pept_deformyl. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700294 / SF370 / Serotype M1.
  2. "Evolutionary origin and emergence of a highly successful clone of serotype M1 group A Streptococcus involved multiple horizontal gene transfer events."
    Sumby P., Porcella S.F., Madrigal A.G., Barbian K.D., Virtaneva K., Ricklefs S.M., Sturdevant D.E., Graham M.R., Vuopio-Varkila J., Hoe N.P., Musser J.M.
    J. Infect. Dis. 192:771-782(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-947 / MGAS5005 / Serotype M1.

Entry informationi

Entry nameiDEF_STRP1
AccessioniPrimary (citable) accession number: P68771
Secondary accession number(s): P82590, Q48WI8, Q99XY7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: December 21, 2004
Last modified: November 26, 2014
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3