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Protein

Cytosol aminopeptidase

Gene

pepA

Organism
Escherichia coli O157:H7
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. Required for plasmid ColE1 site-specific recombination but not in its aminopeptidase activity. Could act as a structural component of the putative nucleoprotein complex in which the Xer recombination reaction takes place (By similarity).By similarity

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.

Cofactori

Mn2+By similarityNote: Binds 2 manganese ions per subunit.By similarity

Enzyme regulationi

Inhibited by zinc and EDTA.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi270Manganese 2By similarity1
Metal bindingi275Manganese 1By similarity1
Metal bindingi275Manganese 2By similarity1
Active sitei282Sequence analysis1
Metal bindingi293Manganese 2By similarity1
Metal bindingi352Manganese 1By similarity1
Metal bindingi354Manganese 1By similarity1
Metal bindingi354Manganese 2By similarity1
Active sitei356Sequence analysis1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

BioCyciECOO157:PEPA-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosol aminopeptidase (EC:3.4.11.1)
Alternative name(s):
Aminopeptidase A/I
Leucine aminopeptidase (EC:3.4.11.10)
Short name:
LAP
Leucyl aminopeptidase
Gene namesi
Name:pepA
Synonyms:carP, xerB
Ordered Locus Names:Z5872, ECs5237
OrganismiEscherichia coli O157:H7
Taxonomic identifieri83334 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000558 Componenti: Chromosome
  • UP000002519 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001657521 – 503Cytosol aminopeptidaseAdd BLAST503

Interactioni

Subunit structurei

Homohexamer.By similarity

Protein-protein interaction databases

STRINGi155864.Z5872.

Structurei

3D structure databases

ProteinModelPortaliP68768.
SMRiP68768.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M17 family.Curated

Phylogenomic databases

eggNOGiENOG4105BZ6. Bacteria.
COG0260. LUCA.
HOGENOMiHOG000243132.
KOiK01255.
OMAiAICEMKL.

Family and domain databases

CDDicd00433. Peptidase_M17. 1 hit.
HAMAPiMF_00181. Cytosol_peptidase_M17. 1 hit.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PANTHERiPTHR11963. PTHR11963. 1 hit.
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P68768-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFSVKSGSP EKQRSACIVV GVFEPRRLSP IAEQLDKISD GYISALLRRG
60 70 80 90 100
ELEGKPGQTL LLHHVPNVLS ERILLIGCGK ERELDERQYK QVIQKTINTL
110 120 130 140 150
NDTGSMEAVC FLTELHVKGR NNYWKVRQAV ETAKETLYSF DQLKTNKSEP
160 170 180 190 200
RRPLRKMVFN VPTRRELTSG ERAIQHGLAI AAGIKAAKDL GNMPPNICNA
210 220 230 240 250
AYLASQARQL ADSYSKNVIT RVIGEQQMKE LGMHSYLAVG QGSQNESLMS
260 270 280 290 300
VIEYKGNASE DARPIVLVGK GLTFDSGGIS IKPSEGMDEM KYDMCGAAAV
310 320 330 340 350
YGVMRMVAEL QLPINVIGVL AGCENMPGGR AYRPGDVLTT MSGQTVEVLN
360 370 380 390 400
TDAEGRLVLC DVLTYVERFE PEAVIDVATL TGACVIALGH HITGLMANHN
410 420 430 440 450
PLAHELIAAS EQSGDRAWRL PLGDEYQEQL ESNFADMANI GGRPGGAITA
460 470 480 490 500
GCFLSRFTRK YNWAHLDIAG TAWRSGKAKG ATGRPVALLA QFLLNRAGFN

GEE
Length:503
Mass (Da):54,880
Last modified:December 21, 2004 - v1
Checksum:i643DED17EAC44DCD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG59459.1.
BA000007 Genomic DNA. Translation: BAB38660.1.
PIRiE91283.
G86124.
RefSeqiNP_313264.1. NC_002695.1.
WP_000397144.1. NZ_LPWC02000002.1.

Genome annotation databases

EnsemblBacteriaiAAG59459; AAG59459; Z5872.
BAB38660; BAB38660; BAB38660.
GeneIDi913804.
KEGGiece:Z5872.
ecs:ECs5237.
PATRICi18360111. VBIEscCol44059_5188.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE005174 Genomic DNA. Translation: AAG59459.1.
BA000007 Genomic DNA. Translation: BAB38660.1.
PIRiE91283.
G86124.
RefSeqiNP_313264.1. NC_002695.1.
WP_000397144.1. NZ_LPWC02000002.1.

3D structure databases

ProteinModelPortaliP68768.
SMRiP68768.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi155864.Z5872.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG59459; AAG59459; Z5872.
BAB38660; BAB38660; BAB38660.
GeneIDi913804.
KEGGiece:Z5872.
ecs:ECs5237.
PATRICi18360111. VBIEscCol44059_5188.

Phylogenomic databases

eggNOGiENOG4105BZ6. Bacteria.
COG0260. LUCA.
HOGENOMiHOG000243132.
KOiK01255.
OMAiAICEMKL.

Enzyme and pathway databases

BioCyciECOO157:PEPA-MONOMER.

Family and domain databases

CDDicd00433. Peptidase_M17. 1 hit.
HAMAPiMF_00181. Cytosol_peptidase_M17. 1 hit.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PANTHERiPTHR11963. PTHR11963. 1 hit.
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAMPA_ECO57
AccessioniPrimary (citable) accession number: P68768
Secondary accession number(s): P11648
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 21, 2004
Last sequence update: December 21, 2004
Last modified: November 2, 2016
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.