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P68761

- HEMA_INCYA

UniProt

P68761 - HEMA_INCYA

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Protein
Hemagglutinin-esterase-fusion glycoprotein
Gene
HE
Organism
Influenza C virus (strain C/Yamagata/10/1981)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Binds to the N-acetyl-9-O-acetylneuraminic acid residues on the cell surface, bringing about the attachment of the virus particle to the cell. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induce an irreversible conformational change in HEF2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore. Displays a receptor-destroying activity which is a neuraminidate-O-acetyl esterase. This activity cleaves off any receptor on the cell surface, which would otherwise prevent virions release. These cleavages prevent self-aggregation and ensure the efficient spread of the progeny virus from cell to cell By similarity.

Catalytic activityi

N-acetyl-O-acetylneuraminate + H2O = N-acetylneuraminate + acetate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei58 – 581Nucleophile By similarity
Active sitei353 – 3531Charge relay system By similarity
Active sitei356 – 3561Charge relay system By similarity

GO - Molecular functioni

  1. sialate O-acetylesterase activity Source: UniProtKB-EC

GO - Biological processi

  1. fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
  2. fusion of virus membrane with host plasma membrane Source: InterPro
  3. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hemagglutinin, Hydrolase

Keywords - Biological processi

Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Hemagglutinin-esterase-fusion glycoprotein (EC:3.1.1.53)
Short name:
HEF
Cleaved into the following 2 chains:
Gene namesi
Name:HE
OrganismiInfluenza C virus (strain C/Yamagata/10/1981)
Taxonomic identifieri11568 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus C
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Sus scrofa (Pig) [TaxID: 9823]

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 617616Extracellular Reviewed prediction
Add
BLAST
Transmembranei618 – 63821Helical; Reviewed prediction
Add
BLAST
Topological domaini639 – 6424Cytoplasmic Reviewed prediction

GO - Cellular componenti

  1. host cell plasma membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. viral envelope Source: UniProtKB-KW
  4. virion membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane, Viral envelope protein, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei‹1 – 1›1
Chaini2 – 433432Hemagglutinin-esterase-fusion glycoprotein chain 1
PRO_0000039170Add
BLAST
Chaini434 – 642209Hemagglutinin-esterase-fusion glycoprotein chain 2
PRO_0000039171Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi7 ↔ 570Interchain (between HEF1 and HEF2 chains) By similarity
Glycosylationi13 – 131N-linked (GlcNAc...); by host Reviewed prediction
Glycosylationi48 – 481N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi107 ↔ 152 By similarity
Disulfide bondi127 ↔ 175 By similarity
Glycosylationi131 – 1311N-linked (GlcNAc...); by host Reviewed prediction
Disulfide bondi197 ↔ 239 By similarity
Disulfide bondi216 ↔ 303 By similarity
Disulfide bondi224 ↔ 276 By similarity
Glycosylationi382 – 3821N-linked (GlcNAc...); by host Reviewed prediction

Post-translational modificationi

In natural infection, inactive HEF is matured into HEF1 and HEF2 outside the cell by one or more trypsin-like, arginine-specific endoprotease By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Homotrimer of disulfide-linked HEF1-HEF2 By similarity.

Structurei

3D structure databases

ProteinModelPortaliP68761.
SMRiP68761. Positions 2-428, 437-598.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 2726Fusion domain-1 By similarity
Add
BLAST
Regioni28 – 138111Esterase domain-1 By similarity
Add
BLAST
Regioni138 – 297160N-acetyl-9-O-acetylneuraminic acid binding By similarity
Add
BLAST
Regioni298 – 35255Esterase domain-2 By similarity
Add
BLAST
Regioni353 – 638286Fusion domain-2 By similarity
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di3.90.20.10. 1 hit.
InterProiIPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
IPR013829. Hemagglutn_stalk.
IPR014831. Hemagglutn_stalk_influenz-C.
[Graphical view]
PfamiPF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
PF08720. Hema_stalk. 1 hit.
[Graphical view]
SUPFAMiSSF49818. SSF49818. 1 hit.

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

P68761-1 [UniParc]FASTAAdd to Basket

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AEKIKICLQK QVNSSFSLHN GFGGNLYATE EKRMFELVKP KAGASVLNQS    50
TWIGFGDSRT DKSNSAFPRS ADVSAKTADK FRSLSGGSLM LSMFGPPGKV 100
DYLYQGCGKH KVFYEGVNWS PHAAINCYRK NWTDIKLNFQ KNIYELASQS 150
HCMSLVNALD KTIPLQATAG VAKNCNNSFL KNPALYTQEV NPSEEICGKE 200
YLAFFTLPTQ FGTYECKLHL VASCYFIYDS KEVYNKRGCD NYFQVIYDSY 250
GKVVGGLDNR VSPYTGNSGD TPTMQCDMLQ LKPGRYSVRS SPRFLLMPER 300
SYCFDMKEKG LVTAVQSIWG KGRESDHAVD QAYLSTPGCM LIQKQKPYIG 350
EADDHHGDQE MRELLSGLDY EARCISQSGW VNETSPFTEE YLLPPKFGRC 400
PLAAKEESIP KIPDGLLIPT SGTDTTVTKP KSRIFGIDDL IIGLFFVAIV 450
EAGIGGYLLG SRKESGGGVT KESAEKGFEK IGNDIQILRS STNIAIEKLN 500
DRISHDEQAI RDLTLEIENA RSEALLGELG IIRALLVGNI SIGLQESLWE 550
LASEITNRAG DLAVEVSPGC WVIDNNICDQ SCQNFIFKFN ETAPVPTIPP 600
LDTKIDLQSD PFYWGSSLGL AITTPISLAA LVISGIAICR TK 642
Length:642
Mass (Da):70,796
Last modified:August 1, 1988 - v1
Checksum:iA66DDC0D8DFAAFA4
GO

Non-terminal residue

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M11641 Genomic RNA. Translation: AAA43786.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M11641 Genomic RNA. Translation: AAA43786.1 .

3D structure databases

ProteinModelPortali P68761.
SMRi P68761. Positions 2-428, 437-598.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.90.20.10. 1 hit.
InterProi IPR008980. Capsid_hemagglutn.
IPR007142. Hemagglutn-estrase_core.
IPR003860. Hemagglutn-estrase_hemagglutn.
IPR013829. Hemagglutn_stalk.
IPR014831. Hemagglutn_stalk_influenz-C.
[Graphical view ]
Pfami PF03996. Hema_esterase. 1 hit.
PF02710. Hema_HEFG. 1 hit.
PF08720. Hema_stalk. 1 hit.
[Graphical view ]
SUPFAMi SSF49818. SSF49818. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Noncumulative sequence changes in the hemagglutinin genes of influenza C virus isolates."
    Buonagurio D.A., Nakada S., Desselberger U., Krystal M., Palese P.
    Virology 146:221-232(1985) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiHEMA_INCYA
AccessioniPrimary (citable) accession number: P68761
Secondary accession number(s): P07969
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: August 1, 1988
Last modified: April 16, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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