P68747 (OTC2_PSESH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ornithine carbamoyltransferase 2, phaseolotoxin-insensitive
Short name=OTCase 2
EC=2.1.3.3

Alternative name(s):
ROCT

Gene names

Name:

argK

Organism

Pseudomonas syringae pv. phaseolicola

Taxonomic identifier

319 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas syringae pv. savastanoi

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Plays an important role in the survival and pathogenicity of P.syringae. Phaseolotoxin is a virulence factor that inhibits the catalysis of the host OTCase. Phaseolotoxin-producing bacteria do not suffer autointoxication because they possess the anabolic OTCase ArgK which can function even in the presence of phaseolotoxin. HAMAP MF_01109
Catalytic activity	Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline. HAMAP MF_01109
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 1/3. HAMAP MF_01109
Induction	By N-sulfodiaminophosphinyl, the inorganic moiety of phaseolotoxin, at 18 degrees Celsius. Ref.4 Ref.5
Miscellaneous	Optimum pH is 8.4. HAMAP MF_01109 This enzyme is insensitive to phaseolotoxin, a potent inhibitor of OTCase. HAMAP MF_01109
Sequence similarities	Belongs to the ATCase/OTCase family.
Caution	Lacks the conserved threonine residue in position 60, which is part of the carbamoylphosphate binding site; it is replaced by a glycine residue.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Molecular function	Transferase
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ornithine carbamoyltransferase complex Inferred from electronic annotation. Source: InterPro
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro ornithine carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 327	326	Ornithine carbamoyltransferase 2, phaseolotoxin-insensitive HAMAP MF_01109		PRO_0000112992
Regions
	Region	58 – 62	5	Carbamoyl phosphate binding By similarity
	Region	272 – 275	4	Ornithine binding By similarity
Sites
	Binding site	109	1	Carbamoyl phosphate By similarity
	Binding site	136	1	Carbamoyl phosphate By similarity
	Site	33	1	Important for structural integrity By similarity
	Site	149	1	Important for structural integrity By similarity
Experimental info
	Sequence conflict	153	1	R → P in CAA39136. Ref.1
	Sequence conflict	293	1	E → Q in CAA39136. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P68747 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 7D31F3A728A7352F	FASTA	327	36,562
	10 20 30 40 50 60 MKITSLKNRN LLTMNEFNQS ELSHLIDRAI ECKRLKKDRI FNLGLNHLNI CGIFLKPSGR 70 80 90 100 110 120 TSTSFVVASY DEGAHFQFFP ADNIRFGHKE SIKDFARVVG RLFDGIAFRG FEHEVAEELA 130 140 150 160 170 180 KHSGIPVWNA LTDTHHPTQV LADVMTVKEE FGRIEGVTIA YVGDGRNNMV TSLAIGALKF 190 200 210 220 230 240 GYNLRIIAPN ALHPTDAVLA GIYEQTPERN GSIEIFTEVA AGVHQADVIY TDVWISMGES 250 260 270 280 290 300 VSVEERIALL KPYKVTEKMM ALTGKADTIF MHCLPAFHDL DTEVARETPD LVEVEDSVFE 310 320 GPQSRVFDQG ENRMHTIKAL MLETVVP

References

[1]	"Isolation and characterization of the gene from Pseudomonas syringae pv. phaseolicola encoding the phaseolotoxin-insensitive ornithine carbamoyltransferase." Mosqueda G., van den Broeck G., Saucedo O., Bailey A.M., Alvarez-Morales A., Herrera-Estrella L. Mol. Gen. Genet. 222:461-466(1990) [PubMed: 2274044] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Isolate Texcoco.
[2]	"Origin, structure, and regulation of argK, encoding the phaseolotoxin-resistant ornithine carbamoyltransferase in Pseudomonas syringae pv. phaseolicola, and functional expression of argK in transgenic tobacco." Hatziloukas E., Panopoulos N.J. J. Bacteriol. 174:5895-5909(1992) [PubMed: 1522066] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NPS 3121.
[3]	"Characterization of two ornithine carbamoyltransferases from Pseudomonas syringae pv. phaseolicola, the producer of phaseolotoxin." Jahn O., Sauerstein J., Reuter G. Arch. Microbiol. 147:174-178(1987) [PubMed: 3592910] [Abstract] Cited for: CHARACTERIZATION.
[4]	"In Pseudomonas syringae pv. phaseolicola, expression of the argK gene, encoding the phaseolotoxin-resistant ornithine carbamoyltransferase, is regulated indirectly by temperature and directly by a precursor resembling carbamoylphosphate." Lopez-Lopez K., Hernandez-Flores J.L., Cruz-Aguilar M., Alvarez-Morales A. J. Bacteriol. 186:146-153(2004) [PubMed: 14679234] [Abstract] Cited for: INDUCTION. Strain: NPS 3121.
[5]	"The global arginine regulator ArgR controls expression of argF in Pseudomonas syringae pv. phaseolicola but is not required for the synthesis of phaseolotoxin or for the regulated expression of argK." Hernandez-Flores J.L., Lopez-Lopez K., Garciduenas-Pina R., Jofre-Garfias A.E., Alvarez-Morales A. J. Bacteriol. 186:3653-3655(2004) [PubMed: 15150254] [Abstract] Cited for: TRANSCRIPTIONAL REGULATION. Strain: NPS 3121.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M94049 Genomic DNA. Translation: AAA25722.1. X55520 Genomic DNA. Translation: CAA39136.1.
PIR	OWPSY. S11943.
3D structure databases
ProteinModelPortal	P68747.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
HAMAP	MF_01109. OTCase. Divergent sequence. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002292. Orn/put_carbamltrans. IPR024904. Orn_carbamltrans. [Graphical view]
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00102. OTCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00658. Orni_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTC2_PSESH

Accession

Primary (citable) accession number: P68747
Secondary accession number(s): P23752

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program