nfi

Functionⁱ

DNA repair enzyme involved in the repair of deaminated bases. Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Has a wide substrate spectrum. In addition to deoxyinosine-containing DNA, the enzyme cleaves DNA containing urea residues, AP sites, base mismatches, insertion/deletion mismatches, flaps, and pseudo-Y structures. Participates in the excision repair of hypoxanthine and xanthine (deaminated adenine and guanine) in DNA. It thereby reduces the mutagenic effects of nitrous acid by attacking lesions caused by nitrosative deamination. Also active on inosines in single- and double-stranded RNA. May cleave tRNA(Arg2), which contains inosine at the wobble position.UniRule annotation5 Publications

Catalytic activityⁱ

Endonucleolytic cleavage at apurinic or apyrimidinic sites to products with a 5'-phosphate.UniRule annotation3 Publications

Cofactorⁱ

Mg²⁺UniRule annotation2 Publications

pH dependenceⁱ

Optimum pH is 7.0-7.5.2 Publications

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Metal bindingⁱ	35	MagnesiumUniRule annotation		1
Metal bindingⁱ	103	MagnesiumUniRule annotation		1

GO - Molecular functionⁱ

deoxyribonuclease V activity
Source: EcoCyc
Inferred from direct assayⁱ
- 14159
metal ion binding Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

DNA repair Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Endonuclease, Hydrolase, Nuclease
Biological process	DNA damage, DNA repair
Ligand	Magnesium, Metal-binding

Enzyme and pathway databases

BioCycⁱ	EcoCyc:EG11915-MONOMER. MetaCyc:EG11915-MONOMER.

BioCycⁱ

EcoCyc:EG11915-MONOMER.
MetaCyc:EG11915-MONOMER.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Endonuclease VUniRule annotation (EC:3.1.21.7UniRule annotation) Short name: EndoV Alternative name(s): Deoxyinosine 3'endonucleaseUniRule annotation Deoxyribonuclease VUniRule annotation Short name: DNase VUniRule annotation
Gene namesⁱ	Name:nfiUniRule annotation Synonyms:yjaF Ordered Locus Names:b3998, JW5547
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG11915. nfi.

EcoGeneⁱ

EG11915. nfi.

Subcellular locationⁱ

Cytoplasm

GO - Cellular componentⁱ

cytoplasm Source: UniProtKB-SubCell

View the complete GO annotation on QuickGO ...

Keywords - Cellular componentⁱ

Cytoplasm

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	35	D → A: Lack of activity on RNA. 1 Publication		1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000159660	1 – 223	Endonuclease VAdd BLAST		223

Proteomic databases

PaxDbⁱ	P68739.
PRIDEⁱ	P68739.

Interactionⁱ

Subunit structureⁱ

Monomer.1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Siteⁱ	73	Interaction with target DNAUniRule annotation		1

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
phrB	P00914	2	EBI-551698,EBI-555781

With

Entry

#Exp.

IntAct

Notes

phrB

P00914

2

EBI-551698,EBI-555781

Protein-protein interaction databases

BioGridⁱ	4263468. 110 interactors.
Database of interacting proteins More...DIPⁱ	DIP-47986N.
IntActⁱ	P68739. 30 interactors.
STRINGⁱ	316385.ECDH10B_4187.

Structureⁱ

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	4XPU	X-ray	2.40	A/B	1-214	[»]
ProteinModelPortalⁱ	P68739.
SMRⁱ	P68739.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the endonuclease V family.UniRule annotation

Phylogenomic databases

eggNOGⁱ	ENOG4105Y7X. Bacteria. COG1515. LUCA.
HOGENOMⁱ	HOG000229135.
InParanoidⁱ	P68739.
KEGG Orthology (KO) More...KOⁱ	K05982.
OMAⁱ	CKYRVPE.
PhylomeDBⁱ	P68739.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd06559. Endonuclease_V. 1 hit.
HAMAPⁱ	MF_00801. Endonuclease_5. 1 hit.
InterProⁱ	View protein in InterPro IPR007581. Endonuclease-V.
PANTHERⁱ	PTHR28511. PTHR28511. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF04493. Endonuclease_5. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

P68739-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MDLASLRAQQ IELASSVIRE DRLDKDPPDL IAGADVGFEQ GGEVTRAAMV 
        60         70         80         90        100
LLKYPSLELV EYKVARIATT MPYIPGFLSF REYPALLAAW EMLSQKPDLV 
       110        120        130        140        150
FVDGHGISHP RRLGVASHFG LLVDVPTIGV AKKRLCGKFE PLSSEPGALA 
       160        170        180        190        200
PLMDKGEQLA WVWRSKARCN PLFIATGHRV SVDSALAWVQ RCMKGYRLPE 
       210        220 
PTRWADAVAS ERPAFVRYTA NQP

Length:223

Mass (Da):24,673

Last modified:December 7, 2004 - v1

Checksum:ⁱA876DEED5EB848E4

GO

Sequence cautionⁱ

The sequence AAC43096 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U00006 Genomic DNA. Translation: AAC43096.1. Different initiation. U00096 Genomic DNA. Translation: AAC76972.2. AP009048 Genomic DNA. Translation: BAE77321.1.
PIRⁱ	A65207.
NCBI Reference Sequences More...RefSeqⁱ	NP_418426.2. NC_000913.3. WP_000362388.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaⁱ	AAC76972; AAC76972; b3998. BAE77321; BAE77321; BAE77321.
GeneIDⁱ	948502.
KEGGⁱ	ecj:JW5547. eco:b3998.
PATRICⁱ	fig\|1411691.4.peg.2713.

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
P68739	B7UPF3 B7MIY4 B7LA92 P68741 B5Z094 B7NRT1 B7N2K0 B7M7Q7 C5A0T9 B1XC01 +138	Escherichia coli O127:H6 (strain E2348/69 / EPEC) Escherichia coli O45:K1 (strain S88 / ExPEC) Escherichia coli (strain 55989 / EAEC) Escherichia coli O157:H7 Escherichia coli O157:H7 (strain EC4115 / EHEC) Escherichia coli O7:K1 (strain IAI39 / ExPEC) Escherichia coli O81 (strain ED1a) Escherichia coli O8 (strain IAI1) Escherichia coli (strain K12 / MC4100 / BW2952) Escherichia coli (strain K12 / DH10B) And more	223	UniRef100_B7UPF3	Cluster: Endonuclease V	149

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
P68739	Q32AH1 E2X3J0 A0A090NXQ5 G5RNK3 UPI0009AF5A50 G5LWP7 G5MR35 A0A2J9LIN3 A0A0X6ZFU4 A0A156J6C1 +769	Shigella dysenteriae serotype 1 (strain Sd197) Shigella dysenteriae 1617 Shigella dysenteriae WRSd3 Salmonella enterica subsp. enterica serovar Uganda str. R8-3404 Salmonella choleraesuis Salmonella enterica subsp. enterica serovar Alachua str. R6-377 Salmonella enterica subsp. enterica serovar Give str. S5-487 Enterobacter hormaechei Enterobacter cloacae complex sp. GN02468 Enterobacter cloacae And more	223	UniRef90_Q32AH1	Cluster: Endonuclease V	780

Protein	Similar proteins	Organisms	Length	Cluster ID	Cluster name	Size
P68739	B4EYT7 A0A2I8JVT8 UPI00019CFF93 UPI00053661CF UPI00073C9509 UPI0006673C9B UPI0006656313 UPI0007DBF110 A0A0J1CC18 UPI000668EFEB +1548	Proteus mirabilis (strain HI4320) Proteus mirabilis Proteus sp. HMSC14B05 Stenotrophomonas maltophilia (Pseudomonas maltophilia) (Xanthomonas maltophilia) Halomonas sp. MC28 Gilliamella sp. A7 Gilliamella sp. N-G4 Gilliamella apicola Gilliamella sp. N-G2 Gilliamella sp. AM1 And more	222	UniRef50_B4EYT7	Cluster: Endonuclease V	1,559

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U00006 Genomic DNA. Translation: AAC43096.1. Different initiation. U00096 Genomic DNA. Translation: AAC76972.2. AP009048 Genomic DNA. Translation: BAE77321.1.
PIRⁱ	A65207.
NCBI Reference Sequences More...RefSeqⁱ	NP_418426.2. NC_000913.3. WP_000362388.1. NZ_LN832404.1.

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	4XPU	X-ray	2.40	A/B	1-214	[»]
ProteinModelPortalⁱ	P68739.
SMRⁱ	P68739.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4263468. 110 interactors.
Database of interacting proteins More...DIPⁱ	DIP-47986N.
IntActⁱ	P68739. 30 interactors.
STRINGⁱ	316385.ECDH10B_4187.

Proteomic databases

PaxDbⁱ	P68739.
PRIDEⁱ	P68739.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC76972; AAC76972; b3998. BAE77321; BAE77321; BAE77321.
GeneIDⁱ	948502.
KEGGⁱ	ecj:JW5547. eco:b3998.
PATRICⁱ	fig\|1411691.4.peg.2713.

Organism-specific databases

EchoBASEⁱ	EB1859.
EcoGeneⁱ	EG11915. nfi.

Phylogenomic databases

eggNOGⁱ	ENOG4105Y7X. Bacteria. COG1515. LUCA.
HOGENOMⁱ	HOG000229135.
InParanoidⁱ	P68739.
KEGG Orthology (KO) More...KOⁱ	K05982.
OMAⁱ	CKYRVPE.
PhylomeDBⁱ	P68739.

Enzyme and pathway databases

BioCycⁱ	EcoCyc:EG11915-MONOMER. MetaCyc:EG11915-MONOMER.

BioCycⁱ

EcoCyc:EG11915-MONOMER.
MetaCyc:EG11915-MONOMER.

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P68739.

PROⁱ

PR:P68739.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd06559. Endonuclease_V. 1 hit.
HAMAPⁱ	MF_00801. Endonuclease_5. 1 hit.
InterProⁱ	View protein in InterPro IPR007581. Endonuclease-V.
PANTHERⁱ	PTHR28511. PTHR28511. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF04493. Endonuclease_5. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	NFI_ECOLI
Accessionⁱ	P68739Primary (citable) accession number: P68739 Secondary accession number(s): P32679, Q2M8T5
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
	Last sequence update:	December 7, 2004
	Last modified:	March 28, 2018
	This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

UniProtKB

UniParc

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UniRef

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Annotation systems

Supporting data

UniProtKB - P68739 (NFI_ECOLI)

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Endonuclease V

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>pH dependencei

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Sites

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

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Functionⁱ

pH dependenceⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ