UniProtKB - P68739 (NFI_ECOLI)
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Protein
Endonuclease V
Gene
nfi
Organism
Escherichia coli (strain K12)
Status
Functioni
DNA repair enzyme involved in the repair of deaminated bases. Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Has a wide substrate spectrum. In addition to deoxyinosine-containing DNA, the enzyme cleaves DNA containing urea residues, AP sites, base mismatches, insertion/deletion mismatches, flaps, and pseudo-Y structures. Participates in the excision repair of hypoxanthine and xanthine (deaminated adenine and guanine) in DNA. It thereby reduces the mutagenic effects of nitrous acid by attacking lesions caused by nitrosative deamination. Also active on inosines in single- and double-stranded RNA. May cleave tRNA(Arg2), which contains inosine at the wobble position.UniRule annotation5 Publications
Catalytic activityi
Endonucleolytic cleavage at apurinic or apyrimidinic sites to products with a 5'-phosphate.UniRule annotation3 Publications
Cofactori
Mg2+UniRule annotation2 Publications
pH dependencei
Optimum pH is 7.0-7.5.2 Publications
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 35 | MagnesiumUniRule annotation | 1 | |
Metal bindingi | 103 | MagnesiumUniRule annotation | 1 |
GO - Molecular functioni
- deoxyribonuclease V activity Source: EcoCyc
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- DNA repair Source: UniProtKB-KW
Keywordsi
Molecular function | Endonuclease, Hydrolase, Nuclease |
Biological process | DNA damage, DNA repair |
Ligand | Magnesium, Metal-binding |
Enzyme and pathway databases
BioCyci | EcoCyc:EG11915-MONOMER. MetaCyc:EG11915-MONOMER. |
Names & Taxonomyi
Protein namesi | Recommended name: Endonuclease VUniRule annotation (EC:3.1.21.7UniRule annotation)Short name: EndoV Alternative name(s): Deoxyinosine 3'endonucleaseUniRule annotation Deoxyribonuclease VUniRule annotation Short name: DNase VUniRule annotation |
Gene namesi | Ordered Locus Names:b3998, JW5547 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Organism-specific databases
EcoGenei | EG11915. nfi. |
Subcellular locationi
GO - Cellular componenti
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 35 | D → A: Lack of activity on RNA. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000159660 | 1 – 223 | Endonuclease VAdd BLAST | 223 |
Proteomic databases
PaxDbi | P68739. |
PRIDEi | P68739. |
Interactioni
Subunit structurei
Monomer.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 73 | Interaction with target DNAUniRule annotation | 1 |
Binary interactionsi
With | Entry | #Exp. | IntAct | Notes |
---|---|---|---|---|
phrB | P00914 | 2 | EBI-551698,EBI-555781 |
Protein-protein interaction databases
BioGridi | 4263468. 110 interactors. |
DIPi | DIP-47986N. |
IntActi | P68739. 30 interactors. |
STRINGi | 316385.ECDH10B_4187. |
Structurei
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4XPU | X-ray | 2.40 | A/B | 1-214 | [»] | |
ProteinModelPortali | P68739. | |||||
SMRi | P68739. | |||||
ModBasei | Search... | |||||
MobiDBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the endonuclease V family.UniRule annotation
Phylogenomic databases
eggNOGi | ENOG4105Y7X. Bacteria. COG1515. LUCA. |
HOGENOMi | HOG000229135. |
InParanoidi | P68739. |
KOi | K05982. |
OMAi | CKYRVPE. |
PhylomeDBi | P68739. |
Family and domain databases
CDDi | cd06559. Endonuclease_V. 1 hit. |
HAMAPi | MF_00801. Endonuclease_5. 1 hit. |
InterProi | View protein in InterPro IPR007581. Endonuclease-V. |
PANTHERi | PTHR28511. PTHR28511. 1 hit. |
Pfami | View protein in Pfam PF04493. Endonuclease_5. 1 hit. |
i Sequence
Sequence statusi: Complete.
P68739-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDLASLRAQQ IELASSVIRE DRLDKDPPDL IAGADVGFEQ GGEVTRAAMV
60 70 80 90 100
LLKYPSLELV EYKVARIATT MPYIPGFLSF REYPALLAAW EMLSQKPDLV
110 120 130 140 150
FVDGHGISHP RRLGVASHFG LLVDVPTIGV AKKRLCGKFE PLSSEPGALA
160 170 180 190 200
PLMDKGEQLA WVWRSKARCN PLFIATGHRV SVDSALAWVQ RCMKGYRLPE
210 220
PTRWADAVAS ERPAFVRYTA NQP
Sequence cautioni
The sequence AAC43096 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00006 Genomic DNA. Translation: AAC43096.1. Different initiation. U00096 Genomic DNA. Translation: AAC76972.2. AP009048 Genomic DNA. Translation: BAE77321.1. |
PIRi | A65207. |
RefSeqi | NP_418426.2. NC_000913.3. WP_000362388.1. NZ_LN832404.1. |
Genome annotation databases
EnsemblBacteriai | AAC76972; AAC76972; b3998. BAE77321; BAE77321; BAE77321. |
GeneIDi | 948502. |
KEGGi | ecj:JW5547. eco:b3998. |
PATRICi | fig|1411691.4.peg.2713. |
Similar proteinsi
Entry informationi
Entry namei | NFI_ECOLI | |
Accessioni | P68739Primary (citable) accession number: P68739 Secondary accession number(s): P32679, Q2M8T5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | December 7, 2004 |
Last sequence update: | December 7, 2004 | |
Last modified: | March 28, 2018 | |
This is version 102 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |