nfi

Functionⁱ

DNA repair enzyme involved in the repair of deaminated bases. Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Has a wide substrate spectrum. In addition to deoxyinosine-containing DNA, the enzyme cleaves DNA containing urea residues, AP sites, base mismatches, insertion/deletion mismatches, flaps, and pseudo-Y structures. Participates in the excision repair of hypoxanthine and xanthine (deaminated adenine and guanine) in DNA. It thereby reduces the mutagenic effects of nitrous acid by attacking lesions caused by nitrosative deamination. Also active on inosines in single- and double-stranded RNA. May cleave tRNA(Arg2), which contains inosine at the wobble position.UniRule annotation5 Publications

Catalytic activityⁱ

Endonucleolytic cleavage at apurinic or apyrimidinic sites to products with a 5'-phosphate.UniRule annotation3 Publications

Cofactorⁱ

Mg²⁺UniRule annotation2 Publications

pH dependenceⁱ

Optimum pH is 7.0-7.5.2 Publications

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Metal bindingⁱ	35	MagnesiumUniRule annotation		1
Metal bindingⁱ	103	MagnesiumUniRule annotation		1

GO - Molecular functionⁱ

deoxyribonuclease V activity
Source: EcoCyc
Inferred from direct assayⁱ
- 14159
metal ion binding Source: UniProtKB-KW

Complete GO annotation...

GO - Biological processⁱ

DNA repair Source: UniProtKB-KW

Complete GO annotation...

Keywordsⁱ

Molecular function	Endonuclease, Hydrolase, Nuclease
Biological process	DNA damage, DNA repair
Ligand	Magnesium, Metal-binding

Enzyme and pathway databases

BioCycⁱ	EcoCyc:EG11915-MONOMER. MetaCyc:EG11915-MONOMER.

BioCycⁱ

EcoCyc:EG11915-MONOMER.
MetaCyc:EG11915-MONOMER.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Endonuclease VUniRule annotation (EC:3.1.21.7UniRule annotation) Short name: EndoV Alternative name(s): Deoxyinosine 3'endonucleaseUniRule annotation Deoxyribonuclease VUniRule annotation Short name: DNase VUniRule annotation
Gene namesⁱ	Name:nfiUniRule annotation Synonyms:yjaF Ordered Locus Names:b3998, JW5547
Organismⁱ	Escherichia coli (strain K12)
Taxonomic identifierⁱ	83333 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › Escherichia coli
Proteomesⁱ	UP000000318 Componentⁱ: Chromosome UP000000625 Componentⁱ: Chromosome

Organism-specific databases

EcoGeneⁱ	EG11915. nfi.

EcoGeneⁱ

EG11915. nfi.

Subcellular locationⁱ

Cytoplasm

GO - Cellular componentⁱ

cytoplasm Source: UniProtKB-SubCell

Complete GO annotation...

Keywords - Cellular componentⁱ

Cytoplasm

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Mutagenesisⁱ	35	D → A: Lack of activity on RNA. 1 Publication		1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000159660	1 – 223	Endonuclease VAdd BLAST		223

Proteomic databases

PaxDbⁱ	P68739.
PRIDEⁱ	P68739.

Interactionⁱ

Subunit structureⁱ

Monomer.1 Publication

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Siteⁱ	73	Interaction with target DNAUniRule annotation		1

Protein-protein interaction databases

BioGridⁱ	4263468. 105 interactors.
Database of interacting proteins More...DIPⁱ	DIP-47986N.
IntActⁱ	P68739. 30 interactors.
Molecular INTeraction database More...MINTⁱ	MINT-1226598.
STRINGⁱ	511145.b3998.

Structureⁱ

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	4XPU	X-ray	2.40	A/B	1-214	[»]
ProteinModelPortalⁱ	P68739.
SMRⁱ	P68739.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the endonuclease V family.UniRule annotation

Phylogenomic databases

eggNOGⁱ	ENOG4105Y7X. Bacteria. COG1515. LUCA.
HOGENOMⁱ	HOG000229135.
InParanoidⁱ	P68739.
KEGG Orthology (KO) More...KOⁱ	K05982.
OMAⁱ	REYPALM.
PhylomeDBⁱ	P68739.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd06559. Endonuclease_V. 1 hit.
HAMAPⁱ	MF_00801. Endonuclease_5. 1 hit.
InterProⁱ	View protein in InterPro IPR007581. Endonuclease-V.
PANTHERⁱ	PTHR28511. PTHR28511. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF04493. Endonuclease_5. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

P68739-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MDLASLRAQQ IELASSVIRE DRLDKDPPDL IAGADVGFEQ GGEVTRAAMV 
        60         70         80         90        100
LLKYPSLELV EYKVARIATT MPYIPGFLSF REYPALLAAW EMLSQKPDLV 
       110        120        130        140        150
FVDGHGISHP RRLGVASHFG LLVDVPTIGV AKKRLCGKFE PLSSEPGALA 
       160        170        180        190        200
PLMDKGEQLA WVWRSKARCN PLFIATGHRV SVDSALAWVQ RCMKGYRLPE 
       210        220 
PTRWADAVAS ERPAFVRYTA NQP

Length:223

Mass (Da):24,673

Last modified:December 7, 2004 - v1

Checksum:ⁱA876DEED5EB848E4

GO

Sequence cautionⁱ

The sequence AAC43096 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U00006 Genomic DNA. Translation: AAC43096.1. Different initiation. U00096 Genomic DNA. Translation: AAC76972.2. AP009048 Genomic DNA. Translation: BAE77321.1.
PIRⁱ	A65207.
NCBI Reference Sequences More...RefSeqⁱ	NP_418426.2. NC_000913.3. WP_000362388.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaⁱ	AAC76972; AAC76972; b3998. BAE77321; BAE77321; BAE77321.
GeneIDⁱ	948502.
KEGGⁱ	ecj:JW5547. eco:b3998.
PATRICⁱ	32123519. VBIEscCol129921_4112.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	U00006 Genomic DNA. Translation: AAC43096.1. Different initiation. U00096 Genomic DNA. Translation: AAC76972.2. AP009048 Genomic DNA. Translation: BAE77321.1.
PIRⁱ	A65207.
NCBI Reference Sequences More...RefSeqⁱ	NP_418426.2. NC_000913.3. WP_000362388.1. NZ_LN832404.1.

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	4XPU	X-ray	2.40	A/B	1-214	[»]
ProteinModelPortalⁱ	P68739.
SMRⁱ	P68739.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	4263468. 105 interactors.
Database of interacting proteins More...DIPⁱ	DIP-47986N.
IntActⁱ	P68739. 30 interactors.
Molecular INTeraction database More...MINTⁱ	MINT-1226598.
STRINGⁱ	511145.b3998.

Proteomic databases

PaxDbⁱ	P68739.
PRIDEⁱ	P68739.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	AAC76972; AAC76972; b3998. BAE77321; BAE77321; BAE77321.
GeneIDⁱ	948502.
KEGGⁱ	ecj:JW5547. eco:b3998.
PATRICⁱ	32123519. VBIEscCol129921_4112.

Organism-specific databases

EchoBASEⁱ	EB1859.
EcoGeneⁱ	EG11915. nfi.

Phylogenomic databases

eggNOGⁱ	ENOG4105Y7X. Bacteria. COG1515. LUCA.
HOGENOMⁱ	HOG000229135.
InParanoidⁱ	P68739.
KEGG Orthology (KO) More...KOⁱ	K05982.
OMAⁱ	REYPALM.
PhylomeDBⁱ	P68739.

Enzyme and pathway databases

BioCycⁱ	EcoCyc:EG11915-MONOMER. MetaCyc:EG11915-MONOMER.

BioCycⁱ

EcoCyc:EG11915-MONOMER.
MetaCyc:EG11915-MONOMER.

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P68739.

PROⁱ

PR:P68739.

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd06559. Endonuclease_V. 1 hit.
HAMAPⁱ	MF_00801. Endonuclease_5. 1 hit.
InterProⁱ	View protein in InterPro IPR007581. Endonuclease-V.
PANTHERⁱ	PTHR28511. PTHR28511. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF04493. Endonuclease_5. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	NFI_ECOLI
Accessionⁱ	P68739Primary (citable) accession number: P68739 Secondary accession number(s): P32679, Q2M8T5
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
	Last sequence update:	December 7, 2004
	Last modified:	March 15, 2017
	This is version 97 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

Escherichia coli
Escherichia coli (strain K12): entries and cross-references to EcoGene
PDB cross-references
Index of Protein Data Bank (PDB) cross-references
SIMILARITY comments
Index of protein domains and families

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P68739 (NFI_ECOLI)

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Endonuclease V

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>pH dependencei

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Sites

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the ‘Sequence’ section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

pH dependenceⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

GO - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Sequence cautionⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ