Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Endonuclease V

Gene

nfi

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

DNA repair enzyme involved in the repair of deaminated bases. Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Has a wide substrate spectrum. In addition to deoxyinosine-containing DNA, the enzyme cleaves DNA containing urea residues, AP sites, base mismatches, insertion/deletion mismatches, flaps, and pseudo-Y structures. Participates in the excision repair of hypoxanthine and xanthine (deaminated adenine and guanine) in DNA. It thereby reduces the mutagenic effects of nitrous acid by attacking lesions caused by nitrosative deamination. Also active on inosines in single- and double-stranded RNA. May cleave tRNA(Arg2), which contains inosine at the wobble position.UniRule annotation5 Publications

Catalytic activityi

Endonucleolytic cleavage at apurinic or apyrimidinic sites to products with a 5'-phosphate.UniRule annotation3 Publications

Cofactori

Mg2+UniRule annotation2 Publications

pH dependencei

Optimum pH is 7.0-7.5.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi35 – 351MagnesiumUniRule annotation
Metal bindingi103 – 1031MagnesiumUniRule annotation

GO - Molecular functioni

  • deoxyribonuclease V activity Source: EcoCyc
  • magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciEcoCyc:EG11915-MONOMER.
ECOL316407:JW5547-MONOMER.
MetaCyc:EG11915-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Endonuclease VUniRule annotation (EC:3.1.21.7UniRule annotation)
Short name:
EndoV
Alternative name(s):
Deoxyinosine 3'endonucleaseUniRule annotation
Deoxyribonuclease VUniRule annotation
Short name:
DNase VUniRule annotation
Gene namesi
Name:nfiUniRule annotation
Synonyms:yjaF
Ordered Locus Names:b3998, JW5547
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11915. nfi.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi35 – 351D → A: Lack of activity on RNA. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 223223Endonuclease VPRO_0000159660Add
BLAST

Proteomic databases

PaxDbiP68739.
PRIDEiP68739.

Interactioni

Subunit structurei

Monomer.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei73 – 731Interaction with target DNAUniRule annotation

Protein-protein interaction databases

BioGridi4263468. 105 interactions.
DIPiDIP-47986N.
IntActiP68739. 30 interactions.
MINTiMINT-1226598.
STRINGi511145.b3998.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4XPUX-ray2.40A/B1-214[»]
ProteinModelPortaliP68739.
SMRiP68739. Positions 1-210.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the endonuclease V family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105Y7X. Bacteria.
COG1515. LUCA.
HOGENOMiHOG000229135.
InParanoidiP68739.
KOiK05982.
OMAiREYPALM.
PhylomeDBiP68739.

Family and domain databases

CDDicd06559. Endonuclease_V. 1 hit.
HAMAPiMF_00801. Endonuclease_5. 1 hit.
InterProiIPR007581. Endonuclease-V.
[Graphical view]
PfamiPF04493. Endonuclease_5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P68739-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDLASLRAQQ IELASSVIRE DRLDKDPPDL IAGADVGFEQ GGEVTRAAMV
60 70 80 90 100
LLKYPSLELV EYKVARIATT MPYIPGFLSF REYPALLAAW EMLSQKPDLV
110 120 130 140 150
FVDGHGISHP RRLGVASHFG LLVDVPTIGV AKKRLCGKFE PLSSEPGALA
160 170 180 190 200
PLMDKGEQLA WVWRSKARCN PLFIATGHRV SVDSALAWVQ RCMKGYRLPE
210 220
PTRWADAVAS ERPAFVRYTA NQP
Length:223
Mass (Da):24,673
Last modified:December 7, 2004 - v1
Checksum:iA876DEED5EB848E4
GO

Sequence cautioni

The sequence AAC43096 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA. Translation: AAC43096.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76972.2.
AP009048 Genomic DNA. Translation: BAE77321.1.
PIRiA65207.
RefSeqiNP_418426.2. NC_000913.3.
WP_000362388.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC76972; AAC76972; b3998.
BAE77321; BAE77321; BAE77321.
GeneIDi948502.
KEGGiecj:JW5547.
eco:b3998.
PATRICi32123519. VBIEscCol129921_4112.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00006 Genomic DNA. Translation: AAC43096.1. Different initiation.
U00096 Genomic DNA. Translation: AAC76972.2.
AP009048 Genomic DNA. Translation: BAE77321.1.
PIRiA65207.
RefSeqiNP_418426.2. NC_000913.3.
WP_000362388.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4XPUX-ray2.40A/B1-214[»]
ProteinModelPortaliP68739.
SMRiP68739. Positions 1-210.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4263468. 105 interactions.
DIPiDIP-47986N.
IntActiP68739. 30 interactions.
MINTiMINT-1226598.
STRINGi511145.b3998.

Proteomic databases

PaxDbiP68739.
PRIDEiP68739.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76972; AAC76972; b3998.
BAE77321; BAE77321; BAE77321.
GeneIDi948502.
KEGGiecj:JW5547.
eco:b3998.
PATRICi32123519. VBIEscCol129921_4112.

Organism-specific databases

EchoBASEiEB1859.
EcoGeneiEG11915. nfi.

Phylogenomic databases

eggNOGiENOG4105Y7X. Bacteria.
COG1515. LUCA.
HOGENOMiHOG000229135.
InParanoidiP68739.
KOiK05982.
OMAiREYPALM.
PhylomeDBiP68739.

Enzyme and pathway databases

BioCyciEcoCyc:EG11915-MONOMER.
ECOL316407:JW5547-MONOMER.
MetaCyc:EG11915-MONOMER.

Miscellaneous databases

PROiP68739.

Family and domain databases

CDDicd06559. Endonuclease_V. 1 hit.
HAMAPiMF_00801. Endonuclease_5. 1 hit.
InterProiIPR007581. Endonuclease-V.
[Graphical view]
PfamiPF04493. Endonuclease_5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNFI_ECOLI
AccessioniPrimary (citable) accession number: P68739
Secondary accession number(s): P32679, Q2M8T5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: December 7, 2004
Last modified: September 7, 2016
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.