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P68736

- NPRE_BACSU

UniProt

P68736 - NPRE_BACSU

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Protein

Bacillolysin

Gene
nprE, BSU14700
Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Extracellular zinc metalloprotease.

Catalytic activityi

Similar, but not identical, to that of thermolysin.

Cofactori

Binds 4 calcium ions per subunit Reviewed prediction.
Binds 1 zinc ion per subunit By similarity.

Temperature dependencei

Thermolabile.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi360 – 3601Calcium 1 Reviewed prediction
Metal bindingi364 – 3641Zinc; catalytic By similarity
Active sitei365 – 3651 By similarity
Metal bindingi368 – 3681Zinc; catalytic By similarity
Metal bindingi388 – 3881Zinc; catalytic By similarity
Metal bindingi399 – 3991Calcium 1 Reviewed prediction
Metal bindingi399 – 3991Calcium 2 Reviewed prediction
Metal bindingi402 – 4021Calcium 1 Reviewed prediction
Metal bindingi402 – 4021Calcium 2 Reviewed prediction
Metal bindingi404 – 4041Calcium 1; via carbonyl oxygen Reviewed prediction
Metal bindingi407 – 4071Calcium 1 Reviewed prediction
Metal bindingi407 – 4071Calcium 2 Reviewed prediction
Active sitei449 – 4491Proton donor By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. metalloendopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciBSUB:BSU14700-MONOMER.

Protein family/group databases

MEROPSiM04.014.

Names & Taxonomyi

Protein namesi
Recommended name:
Bacillolysin (EC:3.4.24.28)
Alternative name(s):
Neutral protease NprE
Gene namesi
Name:nprE
Ordered Locus Names:BSU14700
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU14700. [Micado]

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727 Reviewed predictionAdd
BLAST
Propeptidei28 – 221194Activation peptide By similarityPRO_0000028606Add
BLAST
Chaini222 – 521300BacillolysinPRO_0000028607Add
BLAST

Keywords - PTMi

Zymogen

Proteomic databases

PaxDbiP68736.

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU14700.

Structurei

3D structure databases

ProteinModelPortaliP68736.
SMRiP68736. Positions 222-521.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M4 family.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3227.
HOGENOMiHOG000247250.
KOiK01400.
OMAiDPANWDL.
OrthoDBiEOG6BW4V3.
PhylomeDBiP68736.

Family and domain databases

Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P68736-1 [UniParc]FASTAAdd to Basket

« Hide

MGLGKKLSVA VAASFMSLSI SLPGVQAAEG HQLKENQTNF LSKNAIAQSE    50
LSAPNDKAVK QFLKKNSNIF KGDPSKRLKL VESTTDALGY KHFRYAPVVN 100
GVPIKDSQVI VHVDKSDNVY AVNGELHNQS AAKTDNSQKV SSEKALALAF 150
KAIGKSPDAV SNGAAKNSNK AELKAIETKD GSYRLAYDVT IRYVEPEPAN 200
WEVLVDAETG SILKQQNKVE HAAATGSGTT LKGATVPLNI SYEGGKYVLR 250
DLSKPTGTQI ITYDLQNRQS RLPGTLVSST TKTFTSSSQR AAVDAHYNLG 300
KVYDYFYSNF KRNSYDNKGS KIVSSVHYGT QYNNAAWTGD QMIYGDGDGS 350
FFSPLSGSLD VTAHEMTHGV TQETANLIYE NQPGALNESF SDVFGYFNDT 400
EDWDIGEDIT VSQPALRSLS NPTKYNQPDN YANYRNLPNT DEGDYGGVHT 450
NSGIPNKAAY NTITKLGVSK SQQIYYRALT TYLTPSSTFK DAKAALIQSA 500
RDLYGSTDAA KVEAAWNAVG L 521
Length:521
Mass (Da):56,522
Last modified:August 1, 1988 - v1
Checksum:i439E7B8F19D1E8F4
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti10 – 101A → R1 Publication
Sequence conflicti10 – 101A → R1 Publication
Sequence conflicti44 – 452NA → KP1 Publication
Sequence conflicti44 – 452NA → KP1 Publication
Sequence conflicti77 – 782RL → SV1 Publication
Sequence conflicti77 – 782RL → SV1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K01985 Genomic DNA. Translation: AAA22627.1.
AF012285 Genomic DNA. Translation: AAC24942.1.
U30932 Genomic DNA. Translation: AAA82609.1.
AL009126 Genomic DNA. Translation: CAB13343.1.
Z97025 Genomic DNA. Translation: CAB09705.1.
PIRiA25414. HYBS.
JQ2129.
RefSeqiNP_389353.1. NC_000964.3.
WP_003245026.1. NZ_CM000487.1.

Genome annotation databases

EnsemblBacteriaiCAB13343; CAB13343; BSU14700.
GeneIDi935981.
KEGGibsu:BSU14700.
PATRICi18974737. VBIBacSub10457_1560.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K01985 Genomic DNA. Translation: AAA22627.1 .
AF012285 Genomic DNA. Translation: AAC24942.1 .
U30932 Genomic DNA. Translation: AAA82609.1 .
AL009126 Genomic DNA. Translation: CAB13343.1 .
Z97025 Genomic DNA. Translation: CAB09705.1 .
PIRi A25414. HYBS.
JQ2129.
RefSeqi NP_389353.1. NC_000964.3.
WP_003245026.1. NZ_CM000487.1.

3D structure databases

ProteinModelPortali P68736.
SMRi P68736. Positions 222-521.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224308.BSU14700.

Protein family/group databases

MEROPSi M04.014.

Proteomic databases

PaxDbi P68736.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB13343 ; CAB13343 ; BSU14700 .
GeneIDi 935981.
KEGGi bsu:BSU14700.
PATRICi 18974737. VBIBacSub10457_1560.

Organism-specific databases

GenoListi BSU14700. [Micado ]

Phylogenomic databases

eggNOGi COG3227.
HOGENOMi HOG000247250.
KOi K01400.
OMAi DPANWDL.
OrthoDBi EOG6BW4V3.
PhylomeDBi P68736.

Enzyme and pathway databases

BioCyci BSUB:BSU14700-MONOMER.

Family and domain databases

Gene3Di 3.10.170.10. 1 hit.
InterProi IPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view ]
Pfami PF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view ]
PRINTSi PR00730. THERMOLYSIN.
PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of the neutral protease gene of Bacillus subtilis and the use of the cloned gene to create an in vitro-derived deletion mutation."
    Yang M.Y., Ferrari E., Henner D.J.
    J. Bacteriol. 160:15-21(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The ampS-nprE (124 degrees-127 degrees) region of the Bacillus subtilis 168 chromosome: sequencing of a 27 kb segment and identification of several genes in the area."
    Winters P., Caldwell R.M., Enfield L., Ferrari E.
    Microbiology 142:3033-3037(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  3. Lee S., Yoon K., Nam H., Chae K.
    Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: NS15-4.
  4. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  5. Purnell B., Presecan E., Glaser P., Richou A., Danchin A., Goffeau A.
    Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-158.
    Strain: 168.

Entry informationi

Entry nameiNPRE_BACSU
AccessioniPrimary (citable) accession number: P68736
Secondary accession number(s): P06142, P25268
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: August 1, 1988
Last modified: September 3, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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