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Protein

Bacillolysin

Gene

nprE

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Extracellular zinc metalloprotease.

Catalytic activityi

Similar, but not identical, to that of thermolysin.

Cofactori

Protein has several cofactor binding sites:
  • Ca2+CuratedNote: Binds 4 Ca2+ ions per subunit.Curated
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Temperature dependencei

Thermolabile.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi360Calcium 1Sequence analysis1
Metal bindingi364Zinc; catalyticPROSITE-ProRule annotation1
Active sitei365PROSITE-ProRule annotation1
Metal bindingi368Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi388Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi399Calcium 1Sequence analysis1
Metal bindingi399Calcium 2Sequence analysis1
Metal bindingi402Calcium 1Sequence analysis1
Metal bindingi402Calcium 2Sequence analysis1
Metal bindingi404Calcium 1; via carbonyl oxygenSequence analysis1
Metal bindingi407Calcium 1Sequence analysis1
Metal bindingi407Calcium 2Sequence analysis1
Active sitei449Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciBSUB:BSU14700-MONOMER.
SABIO-RKP68736.

Protein family/group databases

MEROPSiM04.014.

Names & Taxonomyi

Protein namesi
Recommended name:
Bacillolysin (EC:3.4.24.28)
Alternative name(s):
Neutral protease NprE
Gene namesi
Name:nprE
Ordered Locus Names:BSU14700
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
PropeptideiPRO_000002860628 – 221Activation peptideBy similarityAdd BLAST194
ChainiPRO_0000028607222 – 521BacillolysinAdd BLAST300

Keywords - PTMi

Zymogen

Proteomic databases

PaxDbiP68736.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100008146.

Structurei

3D structure databases

ProteinModelPortaliP68736.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M4 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105D4Y. Bacteria.
COG3227. LUCA.
HOGENOMiHOG000247250.
InParanoidiP68736.
KOiK01400.
OMAiVTIRYIE.
PhylomeDBiP68736.

Family and domain databases

CDDicd09597. M4_neutral_protease. 1 hit.
Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P68736-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLGKKLSVA VAASFMSLSI SLPGVQAAEG HQLKENQTNF LSKNAIAQSE
60 70 80 90 100
LSAPNDKAVK QFLKKNSNIF KGDPSKRLKL VESTTDALGY KHFRYAPVVN
110 120 130 140 150
GVPIKDSQVI VHVDKSDNVY AVNGELHNQS AAKTDNSQKV SSEKALALAF
160 170 180 190 200
KAIGKSPDAV SNGAAKNSNK AELKAIETKD GSYRLAYDVT IRYVEPEPAN
210 220 230 240 250
WEVLVDAETG SILKQQNKVE HAAATGSGTT LKGATVPLNI SYEGGKYVLR
260 270 280 290 300
DLSKPTGTQI ITYDLQNRQS RLPGTLVSST TKTFTSSSQR AAVDAHYNLG
310 320 330 340 350
KVYDYFYSNF KRNSYDNKGS KIVSSVHYGT QYNNAAWTGD QMIYGDGDGS
360 370 380 390 400
FFSPLSGSLD VTAHEMTHGV TQETANLIYE NQPGALNESF SDVFGYFNDT
410 420 430 440 450
EDWDIGEDIT VSQPALRSLS NPTKYNQPDN YANYRNLPNT DEGDYGGVHT
460 470 480 490 500
NSGIPNKAAY NTITKLGVSK SQQIYYRALT TYLTPSSTFK DAKAALIQSA
510 520
RDLYGSTDAA KVEAAWNAVG L
Length:521
Mass (Da):56,522
Last modified:August 1, 1988 - v1
Checksum:i439E7B8F19D1E8F4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti10A → R (PubMed:6090407).Curated1
Sequence conflicti10A → R (PubMed:8969500).Curated1
Sequence conflicti44 – 45NA → KP (PubMed:6090407).Curated2
Sequence conflicti44 – 45NA → KP (PubMed:8969500).Curated2
Sequence conflicti77 – 78RL → SV (PubMed:6090407).Curated2
Sequence conflicti77 – 78RL → SV (PubMed:8969500).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01985 Genomic DNA. Translation: AAA22627.1.
AF012285 Genomic DNA. Translation: AAC24942.1.
U30932 Genomic DNA. Translation: AAA82609.1.
AL009126 Genomic DNA. Translation: CAB13343.1.
Z97025 Genomic DNA. Translation: CAB09705.1.
PIRiA25414. HYBS.
JQ2129.
RefSeqiNP_389353.1. NC_000964.3.
WP_003245026.1. NZ_JNCM01000035.1.

Genome annotation databases

EnsemblBacteriaiCAB13343; CAB13343; BSU14700.
GeneIDi935981.
KEGGibsu:BSU14700.
PATRICi18974737. VBIBacSub10457_1560.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K01985 Genomic DNA. Translation: AAA22627.1.
AF012285 Genomic DNA. Translation: AAC24942.1.
U30932 Genomic DNA. Translation: AAA82609.1.
AL009126 Genomic DNA. Translation: CAB13343.1.
Z97025 Genomic DNA. Translation: CAB09705.1.
PIRiA25414. HYBS.
JQ2129.
RefSeqiNP_389353.1. NC_000964.3.
WP_003245026.1. NZ_JNCM01000035.1.

3D structure databases

ProteinModelPortaliP68736.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100008146.

Protein family/group databases

MEROPSiM04.014.

Proteomic databases

PaxDbiP68736.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB13343; CAB13343; BSU14700.
GeneIDi935981.
KEGGibsu:BSU14700.
PATRICi18974737. VBIBacSub10457_1560.

Phylogenomic databases

eggNOGiENOG4105D4Y. Bacteria.
COG3227. LUCA.
HOGENOMiHOG000247250.
InParanoidiP68736.
KOiK01400.
OMAiVTIRYIE.
PhylomeDBiP68736.

Enzyme and pathway databases

BioCyciBSUB:BSU14700-MONOMER.
SABIO-RKP68736.

Family and domain databases

CDDicd09597. M4_neutral_protease. 1 hit.
Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNPRE_BACSU
AccessioniPrimary (citable) accession number: P68736
Secondary accession number(s): P06142, P25268
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: August 1, 1988
Last modified: November 30, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.