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P68736

- NPRE_BACSU

UniProt

P68736 - NPRE_BACSU

Protein

Bacillolysin

Gene

nprE

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Aug 1988)
      Previous versions | rss
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    Functioni

    Extracellular zinc metalloprotease.

    Catalytic activityi

    Similar, but not identical, to that of thermolysin.

    Cofactori

    Binds 4 calcium ions per subunit.Curated
    Binds 1 zinc ion per subunit.By similarity

    Temperature dependencei

    Thermolabile.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi360 – 3601Calcium 1Sequence Analysis
    Metal bindingi364 – 3641Zinc; catalyticPROSITE-ProRule annotation
    Active sitei365 – 3651PROSITE-ProRule annotation
    Metal bindingi368 – 3681Zinc; catalyticPROSITE-ProRule annotation
    Metal bindingi388 – 3881Zinc; catalyticPROSITE-ProRule annotation
    Metal bindingi399 – 3991Calcium 1Sequence Analysis
    Metal bindingi399 – 3991Calcium 2Sequence Analysis
    Metal bindingi402 – 4021Calcium 1Sequence Analysis
    Metal bindingi402 – 4021Calcium 2Sequence Analysis
    Metal bindingi404 – 4041Calcium 1; via carbonyl oxygenSequence Analysis
    Metal bindingi407 – 4071Calcium 1Sequence Analysis
    Metal bindingi407 – 4071Calcium 2Sequence Analysis
    Active sitei449 – 4491Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. metalloendopeptidase activity Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Calcium, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciBSUB:BSU14700-MONOMER.

    Protein family/group databases

    MEROPSiM04.014.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bacillolysin (EC:3.4.24.28)
    Alternative name(s):
    Neutral protease NprE
    Gene namesi
    Name:nprE
    Ordered Locus Names:BSU14700
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU14700. [Micado]

    Subcellular locationi

    Secreted By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Propeptidei28 – 221194Activation peptideBy similarityPRO_0000028606Add
    BLAST
    Chaini222 – 521300BacillolysinPRO_0000028607Add
    BLAST

    Keywords - PTMi

    Zymogen

    Proteomic databases

    PaxDbiP68736.

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU14700.

    Structurei

    3D structure databases

    ProteinModelPortaliP68736.
    SMRiP68736. Positions 222-521.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M4 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3227.
    HOGENOMiHOG000247250.
    KOiK01400.
    OMAiDPANWDL.
    OrthoDBiEOG6BW4V3.
    PhylomeDBiP68736.

    Family and domain databases

    Gene3Di3.10.170.10. 1 hit.
    InterProiIPR011096. FTP_domain.
    IPR025711. PepSY.
    IPR023612. Peptidase_M4.
    IPR001570. Peptidase_M4_C_domain.
    IPR013856. Peptidase_M4_domain.
    [Graphical view]
    PfamiPF07504. FTP. 1 hit.
    PF03413. PepSY. 1 hit.
    PF01447. Peptidase_M4. 1 hit.
    PF02868. Peptidase_M4_C. 1 hit.
    [Graphical view]
    PRINTSiPR00730. THERMOLYSIN.
    PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P68736-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLGKKLSVA VAASFMSLSI SLPGVQAAEG HQLKENQTNF LSKNAIAQSE    50
    LSAPNDKAVK QFLKKNSNIF KGDPSKRLKL VESTTDALGY KHFRYAPVVN 100
    GVPIKDSQVI VHVDKSDNVY AVNGELHNQS AAKTDNSQKV SSEKALALAF 150
    KAIGKSPDAV SNGAAKNSNK AELKAIETKD GSYRLAYDVT IRYVEPEPAN 200
    WEVLVDAETG SILKQQNKVE HAAATGSGTT LKGATVPLNI SYEGGKYVLR 250
    DLSKPTGTQI ITYDLQNRQS RLPGTLVSST TKTFTSSSQR AAVDAHYNLG 300
    KVYDYFYSNF KRNSYDNKGS KIVSSVHYGT QYNNAAWTGD QMIYGDGDGS 350
    FFSPLSGSLD VTAHEMTHGV TQETANLIYE NQPGALNESF SDVFGYFNDT 400
    EDWDIGEDIT VSQPALRSLS NPTKYNQPDN YANYRNLPNT DEGDYGGVHT 450
    NSGIPNKAAY NTITKLGVSK SQQIYYRALT TYLTPSSTFK DAKAALIQSA 500
    RDLYGSTDAA KVEAAWNAVG L 521
    Length:521
    Mass (Da):56,522
    Last modified:August 1, 1988 - v1
    Checksum:i439E7B8F19D1E8F4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti10 – 101A → R(PubMed:6090407)Curated
    Sequence conflicti10 – 101A → R(PubMed:8969500)Curated
    Sequence conflicti44 – 452NA → KP(PubMed:6090407)Curated
    Sequence conflicti44 – 452NA → KP(PubMed:8969500)Curated
    Sequence conflicti77 – 782RL → SV(PubMed:6090407)Curated
    Sequence conflicti77 – 782RL → SV(PubMed:8969500)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K01985 Genomic DNA. Translation: AAA22627.1.
    AF012285 Genomic DNA. Translation: AAC24942.1.
    U30932 Genomic DNA. Translation: AAA82609.1.
    AL009126 Genomic DNA. Translation: CAB13343.1.
    Z97025 Genomic DNA. Translation: CAB09705.1.
    PIRiA25414. HYBS.
    JQ2129.
    RefSeqiNP_389353.1. NC_000964.3.
    WP_003245026.1. NZ_CM000487.1.

    Genome annotation databases

    EnsemblBacteriaiCAB13343; CAB13343; BSU14700.
    GeneIDi935981.
    KEGGibsu:BSU14700.
    PATRICi18974737. VBIBacSub10457_1560.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    K01985 Genomic DNA. Translation: AAA22627.1 .
    AF012285 Genomic DNA. Translation: AAC24942.1 .
    U30932 Genomic DNA. Translation: AAA82609.1 .
    AL009126 Genomic DNA. Translation: CAB13343.1 .
    Z97025 Genomic DNA. Translation: CAB09705.1 .
    PIRi A25414. HYBS.
    JQ2129.
    RefSeqi NP_389353.1. NC_000964.3.
    WP_003245026.1. NZ_CM000487.1.

    3D structure databases

    ProteinModelPortali P68736.
    SMRi P68736. Positions 222-521.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU14700.

    Protein family/group databases

    MEROPSi M04.014.

    Proteomic databases

    PaxDbi P68736.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB13343 ; CAB13343 ; BSU14700 .
    GeneIDi 935981.
    KEGGi bsu:BSU14700.
    PATRICi 18974737. VBIBacSub10457_1560.

    Organism-specific databases

    GenoListi BSU14700. [Micado ]

    Phylogenomic databases

    eggNOGi COG3227.
    HOGENOMi HOG000247250.
    KOi K01400.
    OMAi DPANWDL.
    OrthoDBi EOG6BW4V3.
    PhylomeDBi P68736.

    Enzyme and pathway databases

    BioCyci BSUB:BSU14700-MONOMER.

    Family and domain databases

    Gene3Di 3.10.170.10. 1 hit.
    InterProi IPR011096. FTP_domain.
    IPR025711. PepSY.
    IPR023612. Peptidase_M4.
    IPR001570. Peptidase_M4_C_domain.
    IPR013856. Peptidase_M4_domain.
    [Graphical view ]
    Pfami PF07504. FTP. 1 hit.
    PF03413. PepSY. 1 hit.
    PF01447. Peptidase_M4. 1 hit.
    PF02868. Peptidase_M4_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00730. THERMOLYSIN.
    PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of the neutral protease gene of Bacillus subtilis and the use of the cloned gene to create an in vitro-derived deletion mutation."
      Yang M.Y., Ferrari E., Henner D.J.
      J. Bacteriol. 160:15-21(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The ampS-nprE (124 degrees-127 degrees) region of the Bacillus subtilis 168 chromosome: sequencing of a 27 kb segment and identification of several genes in the area."
      Winters P., Caldwell R.M., Enfield L., Ferrari E.
      Microbiology 142:3033-3037(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    3. Lee S., Yoon K., Nam H., Chae K.
      Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: NS15-4.
    4. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    5. Purnell B., Presecan E., Glaser P., Richou A., Danchin A., Goffeau A.
      Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-158.
      Strain: 168.

    Entry informationi

    Entry nameiNPRE_BACSU
    AccessioniPrimary (citable) accession number: P68736
    Secondary accession number(s): P06142, P25268
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: August 1, 1988
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3