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P68736

- NPRE_BACSU

UniProt

P68736 - NPRE_BACSU

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Protein

Bacillolysin

Gene

nprE

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Extracellular zinc metalloprotease.

Catalytic activityi

Similar, but not identical, to that of thermolysin.

Cofactori

Binds 4 calcium ions per subunit.Curated
Binds 1 zinc ion per subunit.By similarity

Temperature dependencei

Thermolabile.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi360 – 3601Calcium 1Sequence Analysis
Metal bindingi364 – 3641Zinc; catalyticPROSITE-ProRule annotation
Active sitei365 – 3651PROSITE-ProRule annotation
Metal bindingi368 – 3681Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi388 – 3881Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi399 – 3991Calcium 1Sequence Analysis
Metal bindingi399 – 3991Calcium 2Sequence Analysis
Metal bindingi402 – 4021Calcium 1Sequence Analysis
Metal bindingi402 – 4021Calcium 2Sequence Analysis
Metal bindingi404 – 4041Calcium 1; via carbonyl oxygenSequence Analysis
Metal bindingi407 – 4071Calcium 1Sequence Analysis
Metal bindingi407 – 4071Calcium 2Sequence Analysis
Active sitei449 – 4491Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. metalloendopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciBSUB:BSU14700-MONOMER.

Protein family/group databases

MEROPSiM04.014.

Names & Taxonomyi

Protein namesi
Recommended name:
Bacillolysin (EC:3.4.24.28)
Alternative name(s):
Neutral protease NprE
Gene namesi
Name:nprE
Ordered Locus Names:BSU14700
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU14700. [Micado]

Subcellular locationi

Secreted By similarity

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence AnalysisAdd
BLAST
Propeptidei28 – 221194Activation peptideBy similarityPRO_0000028606Add
BLAST
Chaini222 – 521300BacillolysinPRO_0000028607Add
BLAST

Keywords - PTMi

Zymogen

Proteomic databases

PaxDbiP68736.

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU14700.

Structurei

3D structure databases

ProteinModelPortaliP68736.
SMRiP68736. Positions 222-521.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M4 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3227.
HOGENOMiHOG000247250.
InParanoidiP68736.
KOiK01400.
OMAiDPANWDL.
OrthoDBiEOG6BW4V3.
PhylomeDBiP68736.

Family and domain databases

Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P68736-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGLGKKLSVA VAASFMSLSI SLPGVQAAEG HQLKENQTNF LSKNAIAQSE
60 70 80 90 100
LSAPNDKAVK QFLKKNSNIF KGDPSKRLKL VESTTDALGY KHFRYAPVVN
110 120 130 140 150
GVPIKDSQVI VHVDKSDNVY AVNGELHNQS AAKTDNSQKV SSEKALALAF
160 170 180 190 200
KAIGKSPDAV SNGAAKNSNK AELKAIETKD GSYRLAYDVT IRYVEPEPAN
210 220 230 240 250
WEVLVDAETG SILKQQNKVE HAAATGSGTT LKGATVPLNI SYEGGKYVLR
260 270 280 290 300
DLSKPTGTQI ITYDLQNRQS RLPGTLVSST TKTFTSSSQR AAVDAHYNLG
310 320 330 340 350
KVYDYFYSNF KRNSYDNKGS KIVSSVHYGT QYNNAAWTGD QMIYGDGDGS
360 370 380 390 400
FFSPLSGSLD VTAHEMTHGV TQETANLIYE NQPGALNESF SDVFGYFNDT
410 420 430 440 450
EDWDIGEDIT VSQPALRSLS NPTKYNQPDN YANYRNLPNT DEGDYGGVHT
460 470 480 490 500
NSGIPNKAAY NTITKLGVSK SQQIYYRALT TYLTPSSTFK DAKAALIQSA
510 520
RDLYGSTDAA KVEAAWNAVG L
Length:521
Mass (Da):56,522
Last modified:August 1, 1988 - v1
Checksum:i439E7B8F19D1E8F4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti10 – 101A → R(PubMed:6090407)Curated
Sequence conflicti10 – 101A → R(PubMed:8969500)Curated
Sequence conflicti44 – 452NA → KP(PubMed:6090407)Curated
Sequence conflicti44 – 452NA → KP(PubMed:8969500)Curated
Sequence conflicti77 – 782RL → SV(PubMed:6090407)Curated
Sequence conflicti77 – 782RL → SV(PubMed:8969500)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K01985 Genomic DNA. Translation: AAA22627.1.
AF012285 Genomic DNA. Translation: AAC24942.1.
U30932 Genomic DNA. Translation: AAA82609.1.
AL009126 Genomic DNA. Translation: CAB13343.1.
Z97025 Genomic DNA. Translation: CAB09705.1.
PIRiA25414. HYBS.
JQ2129.
RefSeqiNP_389353.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB13343; CAB13343; BSU14700.
GeneIDi935981.
KEGGibsu:BSU14700.
PATRICi18974737. VBIBacSub10457_1560.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
K01985 Genomic DNA. Translation: AAA22627.1 .
AF012285 Genomic DNA. Translation: AAC24942.1 .
U30932 Genomic DNA. Translation: AAA82609.1 .
AL009126 Genomic DNA. Translation: CAB13343.1 .
Z97025 Genomic DNA. Translation: CAB09705.1 .
PIRi A25414. HYBS.
JQ2129.
RefSeqi NP_389353.1. NC_000964.3.

3D structure databases

ProteinModelPortali P68736.
SMRi P68736. Positions 222-521.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224308.BSU14700.

Protein family/group databases

MEROPSi M04.014.

Proteomic databases

PaxDbi P68736.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB13343 ; CAB13343 ; BSU14700 .
GeneIDi 935981.
KEGGi bsu:BSU14700.
PATRICi 18974737. VBIBacSub10457_1560.

Organism-specific databases

GenoListi BSU14700. [Micado ]

Phylogenomic databases

eggNOGi COG3227.
HOGENOMi HOG000247250.
InParanoidi P68736.
KOi K01400.
OMAi DPANWDL.
OrthoDBi EOG6BW4V3.
PhylomeDBi P68736.

Enzyme and pathway databases

BioCyci BSUB:BSU14700-MONOMER.

Family and domain databases

Gene3Di 3.10.170.10. 1 hit.
InterProi IPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view ]
Pfami PF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view ]
PRINTSi PR00730. THERMOLYSIN.
PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of the neutral protease gene of Bacillus subtilis and the use of the cloned gene to create an in vitro-derived deletion mutation."
    Yang M.Y., Ferrari E., Henner D.J.
    J. Bacteriol. 160:15-21(1984) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The ampS-nprE (124 degrees-127 degrees) region of the Bacillus subtilis 168 chromosome: sequencing of a 27 kb segment and identification of several genes in the area."
    Winters P., Caldwell R.M., Enfield L., Ferrari E.
    Microbiology 142:3033-3037(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  3. Lee S., Yoon K., Nam H., Chae K.
    Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: NS15-4.
  4. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  5. Purnell B., Presecan E., Glaser P., Richou A., Danchin A., Goffeau A.
    Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-158.
    Strain: 168.

Entry informationi

Entry nameiNPRE_BACSU
AccessioniPrimary (citable) accession number: P68736
Secondary accession number(s): P06142, P25268
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: August 1, 1988
Last modified: October 29, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3