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P68735

- NPRE_BACSA

UniProt

P68735 - NPRE_BACSA

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Protein

Bacillolysin

Gene
nprE
Organism
Bacillus subtilis subsp. amylosacchariticus
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Extracellular zinc metalloprotease.

Catalytic activityi

Similar, but not identical, to that of thermolysin.

Cofactori

Binds 4 calcium ions per subunit Reviewed prediction.
Binds 1 zinc ion per subunit Reviewed prediction.

Temperature dependencei

Thermolabile.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi360 – 3601Calcium 1 Reviewed prediction
Metal bindingi364 – 3641Zinc; catalytic By similarity
Active sitei365 – 3651 By similarity
Metal bindingi368 – 3681Zinc; catalytic By similarity
Metal bindingi388 – 3881Zinc; catalytic By similarity
Metal bindingi399 – 3991Calcium 1 Reviewed prediction
Metal bindingi399 – 3991Calcium 2 Reviewed prediction
Metal bindingi402 – 4021Calcium 1 Reviewed prediction
Metal bindingi402 – 4021Calcium 2 Reviewed prediction
Metal bindingi404 – 4041Calcium 1; via carbonyl oxygen Reviewed prediction
Metal bindingi407 – 4071Calcium 1 Reviewed prediction
Metal bindingi407 – 4071Calcium 2 Reviewed prediction
Active sitei449 – 4491Proton donor By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. metalloendopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Bacillolysin (EC:3.4.24.28)
Alternative name(s):
MCP 76
Neutral protease
Gene namesi
Name:nprE
OrganismiBacillus subtilis subsp. amylosacchariticus
Taxonomic identifieri1483 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727 Reviewed predictionAdd
BLAST
Propeptidei28 – 221194Activation peptidePRO_0000028604Add
BLAST
Chaini222 – 521300BacillolysinPRO_0000028605Add
BLAST

Keywords - PTMi

Zymogen

Structurei

3D structure databases

ProteinModelPortaliP68735.
SMRiP68735. Positions 222-521.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M4 family.

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P68735-1 [UniParc]FASTAAdd to Basket

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MGLGKKLSVA VAASFMSLSI SLPGVQAAEG HQLKENQTNF LSKNAIAQSE    50
LSAPNDKAVK QFLKKNSNIF KGDPSKRLKL VESTTDALGY KHFRYAPVVN 100
GVPIKDSQVI VHVDKSDNVY AVNGELHNQS AAKTDNSQKV SSEKALALAF 150
KAIGKSPDAV SNGAAKNSNK AELKAIETKD GSYRLAYDVT IRYVEPEPAN 200
WEVLVDAETG SILKQQNKVE HAAATGSGTT LKGATVPLNI SYEGGKYVLR 250
DLSKPTGTQI ITYDLQNRQS RLPGTLVSST TKTFTSSSQR AAVDAHYNLG 300
KVYDYFYSNF KRNSYDNKGS KIVSSVHYGT QYNNAAWTGD QMIYGDGDGS 350
FFSPLSGSLD VTAHEMTHGV TQETANLIYE NQPGALNESF SDVFGYFNDT 400
EDWDIGEDIT VSQPALRSLS NPTKYNQPDN YANYRNLPNT DEGDYGGVHT 450
NSGIPNKAAY NTITKLGVSK SQQIYYRALT TYLTPSSTFK DAKAALIQSA 500
RDLYGSTDAA KVEAAWNAVG L 521
Length:521
Mass (Da):56,522
Last modified:August 1, 1988 - v1
Checksum:i439E7B8F19D1E8F4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10773 Genomic DNA. Translation: BAA01604.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10773 Genomic DNA. Translation: BAA01604.1 .

3D structure databases

ProteinModelPortali P68735.
SMRi P68735. Positions 222-521.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.10.170.10. 1 hit.
InterProi IPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view ]
Pfami PF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view ]
PRINTSi PR00730. THERMOLYSIN.
PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the neutral protease gene from Bacillus subtillis var, amylosacchariticus."
    Yoshimoto T., Oyama H., Takeshita T., Higashi H., Xu S., Tsuru D.
    J. Ferment. Bioeng. 70:370-375(1991)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete amino acid sequence of neutral protease from Bacillus subtilis var. amylosacchariticus."
    Kobayashi R., Yoshimoto T., Tsuru D.
    Agric. Biol. Chem. 53:2737-2749(1989)
    Cited for: PROTEIN SEQUENCE OF 222-521.

Entry informationi

Entry nameiNPRE_BACSA
AccessioniPrimary (citable) accession number: P68735
Secondary accession number(s): P06142, P25268
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: August 1, 1988
Last modified: May 14, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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