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P68735

- NPRE_BACSA

UniProt

P68735 - NPRE_BACSA

Protein

Bacillolysin

Gene

nprE

Organism
Bacillus subtilis subsp. amylosacchariticus
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 1 (01 Aug 1988)
      Previous versions | rss
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    Functioni

    Extracellular zinc metalloprotease.

    Catalytic activityi

    Similar, but not identical, to that of thermolysin.

    Cofactori

    Binds 4 calcium ions per subunit.Curated
    Binds 1 zinc ion per subunit.Curated

    Temperature dependencei

    Thermolabile.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi360 – 3601Calcium 1Sequence Analysis
    Metal bindingi364 – 3641Zinc; catalyticPROSITE-ProRule annotation
    Active sitei365 – 3651PROSITE-ProRule annotation
    Metal bindingi368 – 3681Zinc; catalyticPROSITE-ProRule annotation
    Metal bindingi388 – 3881Zinc; catalyticPROSITE-ProRule annotation
    Metal bindingi399 – 3991Calcium 1Sequence Analysis
    Metal bindingi399 – 3991Calcium 2Sequence Analysis
    Metal bindingi402 – 4021Calcium 1Sequence Analysis
    Metal bindingi402 – 4021Calcium 2Sequence Analysis
    Metal bindingi404 – 4041Calcium 1; via carbonyl oxygenSequence Analysis
    Metal bindingi407 – 4071Calcium 1Sequence Analysis
    Metal bindingi407 – 4071Calcium 2Sequence Analysis
    Active sitei449 – 4491Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. metalloendopeptidase activity Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Calcium, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bacillolysin (EC:3.4.24.28)
    Alternative name(s):
    MCP 76
    Neutral protease
    Gene namesi
    Name:nprE
    OrganismiBacillus subtilis subsp. amylosacchariticus
    Taxonomic identifieri1483 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2727Sequence AnalysisAdd
    BLAST
    Propeptidei28 – 221194Activation peptide1 PublicationPRO_0000028604Add
    BLAST
    Chaini222 – 521300BacillolysinPRO_0000028605Add
    BLAST

    Keywords - PTMi

    Zymogen

    Structurei

    3D structure databases

    ProteinModelPortaliP68735.
    SMRiP68735. Positions 222-521.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M4 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.10.170.10. 1 hit.
    InterProiIPR011096. FTP_domain.
    IPR025711. PepSY.
    IPR023612. Peptidase_M4.
    IPR001570. Peptidase_M4_C_domain.
    IPR013856. Peptidase_M4_domain.
    [Graphical view]
    PfamiPF07504. FTP. 1 hit.
    PF03413. PepSY. 1 hit.
    PF01447. Peptidase_M4. 1 hit.
    PF02868. Peptidase_M4_C. 1 hit.
    [Graphical view]
    PRINTSiPR00730. THERMOLYSIN.
    PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P68735-1 [UniParc]FASTAAdd to Basket

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    MGLGKKLSVA VAASFMSLSI SLPGVQAAEG HQLKENQTNF LSKNAIAQSE    50
    LSAPNDKAVK QFLKKNSNIF KGDPSKRLKL VESTTDALGY KHFRYAPVVN 100
    GVPIKDSQVI VHVDKSDNVY AVNGELHNQS AAKTDNSQKV SSEKALALAF 150
    KAIGKSPDAV SNGAAKNSNK AELKAIETKD GSYRLAYDVT IRYVEPEPAN 200
    WEVLVDAETG SILKQQNKVE HAAATGSGTT LKGATVPLNI SYEGGKYVLR 250
    DLSKPTGTQI ITYDLQNRQS RLPGTLVSST TKTFTSSSQR AAVDAHYNLG 300
    KVYDYFYSNF KRNSYDNKGS KIVSSVHYGT QYNNAAWTGD QMIYGDGDGS 350
    FFSPLSGSLD VTAHEMTHGV TQETANLIYE NQPGALNESF SDVFGYFNDT 400
    EDWDIGEDIT VSQPALRSLS NPTKYNQPDN YANYRNLPNT DEGDYGGVHT 450
    NSGIPNKAAY NTITKLGVSK SQQIYYRALT TYLTPSSTFK DAKAALIQSA 500
    RDLYGSTDAA KVEAAWNAVG L 521
    Length:521
    Mass (Da):56,522
    Last modified:August 1, 1988 - v1
    Checksum:i439E7B8F19D1E8F4
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10773 Genomic DNA. Translation: BAA01604.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D10773 Genomic DNA. Translation: BAA01604.1 .

    3D structure databases

    ProteinModelPortali P68735.
    SMRi P68735. Positions 222-521.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.10.170.10. 1 hit.
    InterProi IPR011096. FTP_domain.
    IPR025711. PepSY.
    IPR023612. Peptidase_M4.
    IPR001570. Peptidase_M4_C_domain.
    IPR013856. Peptidase_M4_domain.
    [Graphical view ]
    Pfami PF07504. FTP. 1 hit.
    PF03413. PepSY. 1 hit.
    PF01447. Peptidase_M4. 1 hit.
    PF02868. Peptidase_M4_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00730. THERMOLYSIN.
    PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the neutral protease gene from Bacillus subtillis var, amylosacchariticus."
      Yoshimoto T., Oyama H., Takeshita T., Higashi H., Xu S., Tsuru D.
      J. Ferment. Bioeng. 70:370-375(1991)
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Complete amino acid sequence of neutral protease from Bacillus subtilis var. amylosacchariticus."
      Kobayashi R., Yoshimoto T., Tsuru D.
      Agric. Biol. Chem. 53:2737-2749(1989)
      Cited for: PROTEIN SEQUENCE OF 222-521.

    Entry informationi

    Entry nameiNPRE_BACSA
    AccessioniPrimary (citable) accession number: P68735
    Secondary accession number(s): P06142, P25268
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: August 1, 1988
    Last modified: October 1, 2014
    This is version 47 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3