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P68735

- NPRE_BACSA

UniProt

P68735 - NPRE_BACSA

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Protein

Bacillolysin

Gene

nprE

Organism
Bacillus subtilis subsp. amylosacchariticus
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Extracellular zinc metalloprotease.

Catalytic activityi

Similar, but not identical, to that of thermolysin.

Cofactori

Binds 4 calcium ions per subunit.Curated
Binds 1 zinc ion per subunit.Curated

Temperature dependencei

Thermolabile.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi360 – 3601Calcium 1Sequence Analysis
Metal bindingi364 – 3641Zinc; catalyticPROSITE-ProRule annotation
Active sitei365 – 3651PROSITE-ProRule annotation
Metal bindingi368 – 3681Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi388 – 3881Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi399 – 3991Calcium 1Sequence Analysis
Metal bindingi399 – 3991Calcium 2Sequence Analysis
Metal bindingi402 – 4021Calcium 1Sequence Analysis
Metal bindingi402 – 4021Calcium 2Sequence Analysis
Metal bindingi404 – 4041Calcium 1; via carbonyl oxygenSequence Analysis
Metal bindingi407 – 4071Calcium 1Sequence Analysis
Metal bindingi407 – 4071Calcium 2Sequence Analysis
Active sitei449 – 4491Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. metalloendopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Bacillolysin (EC:3.4.24.28)
Alternative name(s):
MCP 76
Neutral protease
Gene namesi
Name:nprE
OrganismiBacillus subtilis subsp. amylosacchariticus
Taxonomic identifieri1483 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence AnalysisAdd
BLAST
Propeptidei28 – 221194Activation peptide1 PublicationPRO_0000028604Add
BLAST
Chaini222 – 521300BacillolysinPRO_0000028605Add
BLAST

Keywords - PTMi

Zymogen

Structurei

3D structure databases

ProteinModelPortaliP68735.
SMRiP68735. Positions 222-521.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M4 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.10.170.10. 1 hit.
InterProiIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P68735-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGLGKKLSVA VAASFMSLSI SLPGVQAAEG HQLKENQTNF LSKNAIAQSE
60 70 80 90 100
LSAPNDKAVK QFLKKNSNIF KGDPSKRLKL VESTTDALGY KHFRYAPVVN
110 120 130 140 150
GVPIKDSQVI VHVDKSDNVY AVNGELHNQS AAKTDNSQKV SSEKALALAF
160 170 180 190 200
KAIGKSPDAV SNGAAKNSNK AELKAIETKD GSYRLAYDVT IRYVEPEPAN
210 220 230 240 250
WEVLVDAETG SILKQQNKVE HAAATGSGTT LKGATVPLNI SYEGGKYVLR
260 270 280 290 300
DLSKPTGTQI ITYDLQNRQS RLPGTLVSST TKTFTSSSQR AAVDAHYNLG
310 320 330 340 350
KVYDYFYSNF KRNSYDNKGS KIVSSVHYGT QYNNAAWTGD QMIYGDGDGS
360 370 380 390 400
FFSPLSGSLD VTAHEMTHGV TQETANLIYE NQPGALNESF SDVFGYFNDT
410 420 430 440 450
EDWDIGEDIT VSQPALRSLS NPTKYNQPDN YANYRNLPNT DEGDYGGVHT
460 470 480 490 500
NSGIPNKAAY NTITKLGVSK SQQIYYRALT TYLTPSSTFK DAKAALIQSA
510 520
RDLYGSTDAA KVEAAWNAVG L
Length:521
Mass (Da):56,522
Last modified:August 1, 1988 - v1
Checksum:i439E7B8F19D1E8F4
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10773 Genomic DNA. Translation: BAA01604.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10773 Genomic DNA. Translation: BAA01604.1 .

3D structure databases

ProteinModelPortali P68735.
SMRi P68735. Positions 222-521.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.10.170.10. 1 hit.
InterProi IPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view ]
Pfami PF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view ]
PRINTSi PR00730. THERMOLYSIN.
PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence of the neutral protease gene from Bacillus subtillis var, amylosacchariticus."
    Yoshimoto T., Oyama H., Takeshita T., Higashi H., Xu S., Tsuru D.
    J. Ferment. Bioeng. 70:370-375(1991)
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Complete amino acid sequence of neutral protease from Bacillus subtilis var. amylosacchariticus."
    Kobayashi R., Yoshimoto T., Tsuru D.
    Agric. Biol. Chem. 53:2737-2749(1989)
    Cited for: PROTEIN SEQUENCE OF 222-521.

Entry informationi

Entry nameiNPRE_BACSA
AccessioniPrimary (citable) accession number: P68735
Secondary accession number(s): P06142, P25268
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: August 1, 1988
Last modified: October 29, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3