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P68735 (NPRE_BACSA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bacillolysin

EC=3.4.24.28
Alternative name(s):
MCP 76
Neutral protease
Gene names
Name:nprE
OrganismBacillus subtilis subsp. amylosacchariticus
Taxonomic identifier1483 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length521 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Extracellular zinc metalloprotease.

Catalytic activity

Similar, but not identical, to that of thermolysin.

Cofactor

Binds 4 calcium ions per subunit Potential.

Binds 1 zinc ion per subunit Potential.

Subcellular location

Secreted.

Sequence similarities

Belongs to the peptidase M4 family.

Biophysicochemical properties

Temperature dependence:

Thermolabile.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   LigandCalcium
Metal-binding
Zinc
   Molecular functionHydrolase
Metalloprotease
Protease
   PTMZymogen
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

metalloendopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Propeptide28 – 221194Activation peptide
PRO_0000028604
Chain222 – 521300Bacillolysin
PRO_0000028605

Sites

Active site3651 By similarity
Active site4491Proton donor By similarity
Metal binding3601Calcium 1 Potential
Metal binding3641Zinc; catalytic By similarity
Metal binding3681Zinc; catalytic By similarity
Metal binding3881Zinc; catalytic By similarity
Metal binding3991Calcium 1 Potential
Metal binding3991Calcium 2 Potential
Metal binding4021Calcium 1 Potential
Metal binding4021Calcium 2 Potential
Metal binding4041Calcium 1; via carbonyl oxygen Potential
Metal binding4071Calcium 1 Potential
Metal binding4071Calcium 2 Potential

Sequences

Sequence LengthMass (Da)Tools
P68735 [UniParc].

Last modified August 1, 1988. Version 1.
Checksum: 439E7B8F19D1E8F4

FASTA52156,522
        10         20         30         40         50         60 
MGLGKKLSVA VAASFMSLSI SLPGVQAAEG HQLKENQTNF LSKNAIAQSE LSAPNDKAVK 

        70         80         90        100        110        120 
QFLKKNSNIF KGDPSKRLKL VESTTDALGY KHFRYAPVVN GVPIKDSQVI VHVDKSDNVY 

       130        140        150        160        170        180 
AVNGELHNQS AAKTDNSQKV SSEKALALAF KAIGKSPDAV SNGAAKNSNK AELKAIETKD 

       190        200        210        220        230        240 
GSYRLAYDVT IRYVEPEPAN WEVLVDAETG SILKQQNKVE HAAATGSGTT LKGATVPLNI 

       250        260        270        280        290        300 
SYEGGKYVLR DLSKPTGTQI ITYDLQNRQS RLPGTLVSST TKTFTSSSQR AAVDAHYNLG 

       310        320        330        340        350        360 
KVYDYFYSNF KRNSYDNKGS KIVSSVHYGT QYNNAAWTGD QMIYGDGDGS FFSPLSGSLD 

       370        380        390        400        410        420 
VTAHEMTHGV TQETANLIYE NQPGALNESF SDVFGYFNDT EDWDIGEDIT VSQPALRSLS 

       430        440        450        460        470        480 
NPTKYNQPDN YANYRNLPNT DEGDYGGVHT NSGIPNKAAY NTITKLGVSK SQQIYYRALT 

       490        500        510        520 
TYLTPSSTFK DAKAALIQSA RDLYGSTDAA KVEAAWNAVG L 

« Hide

References

[1]"Nucleotide sequence of the neutral protease gene from Bacillus subtillis var, amylosacchariticus."
Yoshimoto T., Oyama H., Takeshita T., Higashi H., Xu S., Tsuru D.
J. Ferment. Bioeng. 70:370-375(1991)
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Complete amino acid sequence of neutral protease from Bacillus subtilis var. amylosacchariticus."
Kobayashi R., Yoshimoto T., Tsuru D.
Agric. Biol. Chem. 53:2737-2749(1989)
Cited for: PROTEIN SEQUENCE OF 222-521.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D10773 Genomic DNA. Translation: BAA01604.1.

3D structure databases

ProteinModelPortalP68735.
SMRP68735. Positions 222-521.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.10.170.10. 1 hit.
InterProIPR011096. FTP_domain.
IPR025711. PepSY.
IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamPF07504. FTP. 1 hit.
PF03413. PepSY. 1 hit.
PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSPR00730. THERMOLYSIN.
PROSITEPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNPRE_BACSA
AccessionPrimary (citable) accession number: P68735
Secondary accession number(s): P06142, P25268
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: August 1, 1988
Last modified: May 14, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries