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Protein

Neutral protease NprE

Gene

nprE

Organism
Bacillus pumilus (Bacillus mesentericus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Extracellular zinc metalloprotease.

Catalytic activityi

Similar, but not identical, to that of thermolysin.

Cofactori

Protein has several cofactor binding sites:
  • Ca2+CuratedNote: Binds 4 Ca2+ ions per subunit.Curated
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Temperature dependencei

Thermolabile.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi139Calcium 1Sequence analysis1
Metal bindingi143Zinc; catalyticPROSITE-ProRule annotation1
Active sitei144PROSITE-ProRule annotation1
Metal bindingi147Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi167Zinc; catalyticPROSITE-ProRule annotation1
Metal bindingi178Calcium 1Sequence analysis1
Metal bindingi178Calcium 2Sequence analysis1
Metal bindingi181Calcium 1Sequence analysis1
Metal bindingi181Calcium 2Sequence analysis1
Metal bindingi183Calcium 1; via carbonyl oxygenSequence analysis1
Metal bindingi186Calcium 1Sequence analysis1
Metal bindingi186Calcium 2Sequence analysis1
Active sitei228Proton donorPROSITE-ProRule annotation1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Neutral protease NprE (EC:3.4.24.28)
Alternative name(s):
Bacillolysin-like protease
Milk-clotting protease from Bacillus mesentericus strain 76
Short name:
MCP 76
Gene namesi
Name:nprE
OrganismiBacillus pumilus (Bacillus mesentericus)
Taxonomic identifieri1408 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000781731 – 300Neutral protease NprEAdd BLAST300

Structurei

3D structure databases

ProteinModelPortaliP68734.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M4 family.Curated

Family and domain databases

CDDicd09597. M4_neutral_protease. 1 hit.
Gene3Di3.10.170.10. 1 hit.
InterProiIPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P68734-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
AAATGSGTTL KGATVPLNIS YEGGKYVLRD LSKPTGTQII TYDLQNRQSR
60 70 80 90 100
LPGTLVSSTT KTFTSSSQRA AVDAHYNLGK VYDYFYSNFK RNSYDNKGSK
110 120 130 140 150
IVSSVHYGTQ YNNAAWTGDQ MIYGDGDGSF FSPLSGSLDV TAHEMTHGVT
160 170 180 190 200
QETANLIYEN QPGALNESFS DVFGYFNDTE DWDIGEDITV SQPALRSLSN
210 220 230 240 250
PTKYNQPDNY ANYRNLPNTD EGDYGGVHTN SGIPNKAAYN TITKLGVSKS
260 270 280 290 300
QQIYYRALTT YLTPSSTFKD AKAALIQSAR DLYGSTDAAK VEAAWNAVGL
Length:300
Mass (Da):32,674
Last modified:December 7, 2004 - v1
Checksum:i6207B816C446385B
GO

Cross-referencesi

3D structure databases

ProteinModelPortaliP68734.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

CDDicd09597. M4_neutral_protease. 1 hit.
Gene3Di3.10.170.10. 1 hit.
InterProiIPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNPRE_BACPU
AccessioniPrimary (citable) accession number: P68734
Secondary accession number(s): P06142, P25268
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: December 7, 2004
Last modified: November 30, 2016
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.