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P68734 (NPRE_BACPU) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neutral protease NprE

EC=3.4.24.28
Alternative name(s):
Bacillolysin-like protease
Milk-clotting protease from Bacillus mesentericus strain 76
Short name=MCP 76
Gene names
Name:nprE
OrganismBacillus pumilus (Bacillus mesentericus)
Taxonomic identifier1408 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length300 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Extracellular zinc metalloprotease.

Catalytic activity

Similar, but not identical, to that of thermolysin.

Cofactor

Binds 4 calcium ions per subunit Potential.

Binds 1 zinc ion per subunit By similarity.

Subcellular location

Secreted.

Sequence similarities

Belongs to the peptidase M4 family.

Biophysicochemical properties

Temperature dependence:

Thermolabile.

Ontologies

Keywords
   Cellular componentSecreted
   LigandCalcium
Metal-binding
Zinc
   Molecular functionHydrolase
Metalloprotease
Protease
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

metalloendopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 300300Neutral protease NprE
PRO_0000078173

Sites

Active site1441 By similarity
Active site2281Proton donor By similarity
Metal binding1391Calcium 1 Potential
Metal binding1431Zinc; catalytic By similarity
Metal binding1471Zinc; catalytic By similarity
Metal binding1671Zinc; catalytic By similarity
Metal binding1781Calcium 1 Potential
Metal binding1781Calcium 2 Potential
Metal binding1811Calcium 1 Potential
Metal binding1811Calcium 2 Potential
Metal binding1831Calcium 1; via carbonyl oxygen Potential
Metal binding1861Calcium 1 Potential
Metal binding1861Calcium 2 Potential

Sequences

Sequence LengthMass (Da)Tools
P68734 [UniParc].

Last modified December 7, 2004. Version 1.
Checksum: 6207B816C446385B

FASTA30032,674
        10         20         30         40         50         60 
AAATGSGTTL KGATVPLNIS YEGGKYVLRD LSKPTGTQII TYDLQNRQSR LPGTLVSSTT 

        70         80         90        100        110        120 
KTFTSSSQRA AVDAHYNLGK VYDYFYSNFK RNSYDNKGSK IVSSVHYGTQ YNNAAWTGDQ 

       130        140        150        160        170        180 
MIYGDGDGSF FSPLSGSLDV TAHEMTHGVT QETANLIYEN QPGALNESFS DVFGYFNDTE 

       190        200        210        220        230        240 
DWDIGEDITV SQPALRSLSN PTKYNQPDNY ANYRNLPNTD EGDYGGVHTN SGIPNKAAYN 

       250        260        270        280        290        300 
TITKLGVSKS QQIYYRALTT YLTPSSTFKD AKAALIQSAR DLYGSTDAAK VEAAWNAVGL 

« Hide

References

[1]"Primary structure of a zinc protease from Bacillus mesentericus strain 76."
Stoeva S., Kleinschmidt T., Mesrob B., Braunitzer G.
Biochemistry 29:527-534(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE.
Strain: 76.

Cross-references

3D structure databases

ProteinModelPortalP68734.
SMRP68734. Positions 1-300.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSM04.014.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.10.170.10. 1 hit.
InterProIPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamPF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSPR00730. THERMOLYSIN.
PROSITEPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNPRE_BACPU
AccessionPrimary (citable) accession number: P68734
Secondary accession number(s): P06142, P25268
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: December 7, 2004
Last modified: May 14, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries