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P68734

- NPRE_BACPU

UniProt

P68734 - NPRE_BACPU

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Protein
Neutral protease NprE
Gene
nprE
Organism
Bacillus pumilus (Bacillus mesentericus)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Extracellular zinc metalloprotease.

Catalytic activityi

Similar, but not identical, to that of thermolysin.

Cofactori

Binds 4 calcium ions per subunit Reviewed prediction.
Binds 1 zinc ion per subunit By similarity.

Temperature dependencei

Thermolabile.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi139 – 1391Calcium 1 Reviewed prediction
Metal bindingi143 – 1431Zinc; catalytic By similarity
Active sitei144 – 1441 By similarity
Metal bindingi147 – 1471Zinc; catalytic By similarity
Metal bindingi167 – 1671Zinc; catalytic By similarity
Metal bindingi178 – 1781Calcium 1 Reviewed prediction
Metal bindingi178 – 1781Calcium 2 Reviewed prediction
Metal bindingi181 – 1811Calcium 1 Reviewed prediction
Metal bindingi181 – 1811Calcium 2 Reviewed prediction
Metal bindingi183 – 1831Calcium 1; via carbonyl oxygen Reviewed prediction
Metal bindingi186 – 1861Calcium 1 Reviewed prediction
Metal bindingi186 – 1861Calcium 2 Reviewed prediction
Active sitei228 – 2281Proton donor By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. metalloendopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiM04.014.

Names & Taxonomyi

Protein namesi
Recommended name:
Neutral protease NprE (EC:3.4.24.28)
Alternative name(s):
Bacillolysin-like protease
Milk-clotting protease from Bacillus mesentericus strain 76
Short name:
MCP 76
Gene namesi
Name:nprE
OrganismiBacillus pumilus (Bacillus mesentericus)
Taxonomic identifieri1408 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 300300Neutral protease NprE
PRO_0000078173Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP68734.
SMRiP68734. Positions 1-300.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M4 family.

Family and domain databases

Gene3Di3.10.170.10. 1 hit.
InterProiIPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P68734-1 [UniParc]FASTAAdd to Basket

« Hide

AAATGSGTTL KGATVPLNIS YEGGKYVLRD LSKPTGTQII TYDLQNRQSR    50
LPGTLVSSTT KTFTSSSQRA AVDAHYNLGK VYDYFYSNFK RNSYDNKGSK 100
IVSSVHYGTQ YNNAAWTGDQ MIYGDGDGSF FSPLSGSLDV TAHEMTHGVT 150
QETANLIYEN QPGALNESFS DVFGYFNDTE DWDIGEDITV SQPALRSLSN 200
PTKYNQPDNY ANYRNLPNTD EGDYGGVHTN SGIPNKAAYN TITKLGVSKS 250
QQIYYRALTT YLTPSSTFKD AKAALIQSAR DLYGSTDAAK VEAAWNAVGL 300
Length:300
Mass (Da):32,674
Last modified:December 7, 2004 - v1
Checksum:i6207B816C446385B
GO

Cross-referencesi

3D structure databases

ProteinModelPortali P68734.
SMRi P68734. Positions 1-300.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi M04.014.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.10.170.10. 1 hit.
InterProi IPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view ]
Pfami PF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view ]
PRINTSi PR00730. THERMOLYSIN.
PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Primary structure of a zinc protease from Bacillus mesentericus strain 76."
    Stoeva S., Kleinschmidt T., Mesrob B., Braunitzer G.
    Biochemistry 29:527-534(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
    Strain: 76.

Entry informationi

Entry nameiNPRE_BACPU
AccessioniPrimary (citable) accession number: P68734
Secondary accession number(s): P06142, P25268
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: December 7, 2004
Last modified: May 14, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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