Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P68734

- NPRE_BACPU

UniProt

P68734 - NPRE_BACPU

Protein

Neutral protease NprE

Gene

nprE

Organism
Bacillus pumilus (Bacillus mesentericus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 1 (07 Dec 2004)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Extracellular zinc metalloprotease.

    Catalytic activityi

    Similar, but not identical, to that of thermolysin.

    Cofactori

    Binds 4 calcium ions per subunit.Curated
    Binds 1 zinc ion per subunit.By similarity

    Temperature dependencei

    Thermolabile.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi139 – 1391Calcium 1Sequence Analysis
    Metal bindingi143 – 1431Zinc; catalyticPROSITE-ProRule annotation
    Active sitei144 – 1441PROSITE-ProRule annotation
    Metal bindingi147 – 1471Zinc; catalyticPROSITE-ProRule annotation
    Metal bindingi167 – 1671Zinc; catalyticPROSITE-ProRule annotation
    Metal bindingi178 – 1781Calcium 1Sequence Analysis
    Metal bindingi178 – 1781Calcium 2Sequence Analysis
    Metal bindingi181 – 1811Calcium 1Sequence Analysis
    Metal bindingi181 – 1811Calcium 2Sequence Analysis
    Metal bindingi183 – 1831Calcium 1; via carbonyl oxygenSequence Analysis
    Metal bindingi186 – 1861Calcium 1Sequence Analysis
    Metal bindingi186 – 1861Calcium 2Sequence Analysis
    Active sitei228 – 2281Proton donorPROSITE-ProRule annotation

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. metalloendopeptidase activity Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Calcium, Metal-binding, Zinc

    Protein family/group databases

    MEROPSiM04.014.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Neutral protease NprE (EC:3.4.24.28)
    Alternative name(s):
    Bacillolysin-like protease
    Milk-clotting protease from Bacillus mesentericus strain 76
    Short name:
    MCP 76
    Gene namesi
    Name:nprE
    OrganismiBacillus pumilus (Bacillus mesentericus)
    Taxonomic identifieri1408 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 300300Neutral protease NprEPRO_0000078173Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP68734.
    SMRiP68734. Positions 1-300.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M4 family.Curated

    Family and domain databases

    Gene3Di3.10.170.10. 1 hit.
    InterProiIPR023612. Peptidase_M4.
    IPR001570. Peptidase_M4_C_domain.
    IPR013856. Peptidase_M4_domain.
    [Graphical view]
    PfamiPF01447. Peptidase_M4. 1 hit.
    PF02868. Peptidase_M4_C. 1 hit.
    [Graphical view]
    PRINTSiPR00730. THERMOLYSIN.
    PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P68734-1 [UniParc]FASTAAdd to Basket

    « Hide

    AAATGSGTTL KGATVPLNIS YEGGKYVLRD LSKPTGTQII TYDLQNRQSR    50
    LPGTLVSSTT KTFTSSSQRA AVDAHYNLGK VYDYFYSNFK RNSYDNKGSK 100
    IVSSVHYGTQ YNNAAWTGDQ MIYGDGDGSF FSPLSGSLDV TAHEMTHGVT 150
    QETANLIYEN QPGALNESFS DVFGYFNDTE DWDIGEDITV SQPALRSLSN 200
    PTKYNQPDNY ANYRNLPNTD EGDYGGVHTN SGIPNKAAYN TITKLGVSKS 250
    QQIYYRALTT YLTPSSTFKD AKAALIQSAR DLYGSTDAAK VEAAWNAVGL 300
    Length:300
    Mass (Da):32,674
    Last modified:December 7, 2004 - v1
    Checksum:i6207B816C446385B
    GO

    Cross-referencesi

    3D structure databases

    ProteinModelPortali P68734.
    SMRi P68734. Positions 1-300.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi M04.014.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.10.170.10. 1 hit.
    InterProi IPR023612. Peptidase_M4.
    IPR001570. Peptidase_M4_C_domain.
    IPR013856. Peptidase_M4_domain.
    [Graphical view ]
    Pfami PF01447. Peptidase_M4. 1 hit.
    PF02868. Peptidase_M4_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00730. THERMOLYSIN.
    PROSITEi PS00142. ZINC_PROTEASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of a zinc protease from Bacillus mesentericus strain 76."
      Stoeva S., Kleinschmidt T., Mesrob B., Braunitzer G.
      Biochemistry 29:527-534(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE.
      Strain: 76.

    Entry informationi

    Entry nameiNPRE_BACPU
    AccessioniPrimary (citable) accession number: P68734
    Secondary accession number(s): P06142, P25268
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 1, 1988
    Last sequence update: December 7, 2004
    Last modified: October 1, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3