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Reviewed, UniProtKB/Swiss-Prot P68734 (NPRE_BACPU)

Last modified June 16, 2009. Version 29. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Bacillolysin
    EC=3.4.24.28
Alternative name(s):
    Neutral protease
    MCP 76
Gene names
Name: nprE
OrganismBacillus pumilus (Bacillus mesentericus)
Taxonomic identifier1408 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

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Protein attributes

Sequence length300 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Extracellular zinc metalloprotease.

Catalytic activity

Similar, but not identical, to that of thermolysin.

Cofactor

Binds 4 calcium ions per subunit Potential.

Binds 1 zinc ion per subunit Potential.

Subcellular location

Secreted.

Sequence similarities

Belongs to the peptidase M4 family.

biophysicochemical properties

Temperature dependence:

Thermolabile.

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Ontologies

Keywords
   Cellular componentSecreted
   LigandCalcium
Metal-binding
Zinc
   Molecular functionHydrolase
Metalloprotease
Protease
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processproteolysis

Inferred from electronic annotation. Source: InterPro

   Cellular componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncalcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

metalloendopeptidase activity

Inferred from electronic annotation. Source: InterPro

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 300300Bacillolysin
PRO_0000078173

Sites

Active site1441 By similarity
Active site2281Proton donor By similarity
Metal binding1391Calcium 1 Potential
Metal binding1431Zinc; catalytic By similarity
Metal binding1471Zinc; catalytic By similarity
Metal binding1671Zinc; catalytic By similarity
Metal binding1781Calcium 1 Potential
Metal binding1781Calcium 2 Potential
Metal binding1811Calcium 1 Potential
Metal binding1811Calcium 2 Potential
Metal binding1831Calcium 1; via carbonyl oxygen Potential
Metal binding1861Calcium 1 Potential
Metal binding1861Calcium 2 Potential

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Sequences

Sequence LengthMass (Da)Tools
P68734-1 [UniParc].

Last modified December 7, 2004. Version 1.
Checksum: 6207B816C446385B

FASTA30032,674
        10         20         30         40         50         60 
AAATGSGTTL KGATVPLNIS YEGGKYVLRD LSKPTGTQII TYDLQNRQSR LPGTLVSSTT 

        70         80         90        100        110        120 
KTFTSSSQRA AVDAHYNLGK VYDYFYSNFK RNSYDNKGSK IVSSVHYGTQ YNNAAWTGDQ 

       130        140        150        160        170        180 
MIYGDGDGSF FSPLSGSLDV TAHEMTHGVT QETANLIYEN QPGALNESFS DVFGYFNDTE 

       190        200        210        220        230        240 
DWDIGEDITV SQPALRSLSN PTKYNQPDNY ANYRNLPNTD EGDYGGVHTN SGIPNKAAYN 

       250        260        270        280        290        300 
TITKLGVSKS QQIYYRALTT YLTPSSTFKD AKAALIQSAR DLYGSTDAAK VEAAWNAVGL

« Hide

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References

[1]"Primary structure of a zinc protease from Bacillus mesentericus strain 76."
Stoeva S., Kleinschmidt T., Mesrob B., Braunitzer G.
Biochemistry 29:527-534(1990) [PubMed: 2302386] [Abstract]
Cited for: PROTEIN SEQUENCE.
Strain: 76.

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Cross-references

3D structure databases

HSSPHSSP built from PDB template 1BQB based on UniProtKB P81177.
ModBaseSearch...

Protein family/group databases

MEROPSM04.014.

Enzyme and pathway databases

BRENDA3.4.24.28. 1189.

Family and domain databases

InterProIPR006025. Pept_M_Zn_BS.
IPR013856. Peptidase_M4.
IPR001570. Peptidase_M4_C.
[Graphical view]
Gene3DG3DSA:3.10.170.10. Peptidase_M4. 1 hit.
G3DSA:1.10.390.10. Peptidase_M4/M36. 1 hit.
PfamPF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSPR00730. THERMOLYSIN.
PROSITEPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameNPRE_BACPU
AccessionPrimary (citable) accession number: P68734
Secondary accession number(s): P06142, P25268
Entry history
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: December 7, 2004
Last modified: June 16, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents