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Protein

Neutral protease NprE

Gene

nprE

Organism
Bacillus pumilus (Bacillus mesentericus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Extracellular zinc metalloprotease.

Catalytic activityi

Similar, but not identical, to that of thermolysin.

Cofactori

Protein has several cofactor binding sites:
  • Ca2+CuratedNote: Binds 4 Ca2+ ions per subunit.Curated
  • Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Temperature dependencei

Thermolabile.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi139 – 1391Calcium 1Sequence Analysis
Metal bindingi143 – 1431Zinc; catalyticPROSITE-ProRule annotation
Active sitei144 – 1441PROSITE-ProRule annotation
Metal bindingi147 – 1471Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi167 – 1671Zinc; catalyticPROSITE-ProRule annotation
Metal bindingi178 – 1781Calcium 1Sequence Analysis
Metal bindingi178 – 1781Calcium 2Sequence Analysis
Metal bindingi181 – 1811Calcium 1Sequence Analysis
Metal bindingi181 – 1811Calcium 2Sequence Analysis
Metal bindingi183 – 1831Calcium 1; via carbonyl oxygenSequence Analysis
Metal bindingi186 – 1861Calcium 1Sequence Analysis
Metal bindingi186 – 1861Calcium 2Sequence Analysis
Active sitei228 – 2281Proton donorPROSITE-ProRule annotation

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. metalloendopeptidase activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Calcium, Metal-binding, Zinc

Protein family/group databases

MEROPSiM04.014.

Names & Taxonomyi

Protein namesi
Recommended name:
Neutral protease NprE (EC:3.4.24.28)
Alternative name(s):
Bacillolysin-like protease
Milk-clotting protease from Bacillus mesentericus strain 76
Short name:
MCP 76
Gene namesi
Name:nprE
OrganismiBacillus pumilus (Bacillus mesentericus)
Taxonomic identifieri1408 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 300300Neutral protease NprEPRO_0000078173Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP68734.
SMRiP68734. Positions 1-300.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M4 family.Curated

Family and domain databases

Gene3Di3.10.170.10. 1 hit.
InterProiIPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P68734-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
AAATGSGTTL KGATVPLNIS YEGGKYVLRD LSKPTGTQII TYDLQNRQSR
60 70 80 90 100
LPGTLVSSTT KTFTSSSQRA AVDAHYNLGK VYDYFYSNFK RNSYDNKGSK
110 120 130 140 150
IVSSVHYGTQ YNNAAWTGDQ MIYGDGDGSF FSPLSGSLDV TAHEMTHGVT
160 170 180 190 200
QETANLIYEN QPGALNESFS DVFGYFNDTE DWDIGEDITV SQPALRSLSN
210 220 230 240 250
PTKYNQPDNY ANYRNLPNTD EGDYGGVHTN SGIPNKAAYN TITKLGVSKS
260 270 280 290 300
QQIYYRALTT YLTPSSTFKD AKAALIQSAR DLYGSTDAAK VEAAWNAVGL
Length:300
Mass (Da):32,674
Last modified:December 7, 2004 - v1
Checksum:i6207B816C446385B
GO

Cross-referencesi

3D structure databases

ProteinModelPortaliP68734.
SMRiP68734. Positions 1-300.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiM04.014.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.10.170.10. 1 hit.
InterProiIPR023612. Peptidase_M4.
IPR001570. Peptidase_M4_C_domain.
IPR013856. Peptidase_M4_domain.
[Graphical view]
PfamiPF01447. Peptidase_M4. 1 hit.
PF02868. Peptidase_M4_C. 1 hit.
[Graphical view]
PRINTSiPR00730. THERMOLYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Primary structure of a zinc protease from Bacillus mesentericus strain 76."
    Stoeva S., Kleinschmidt T., Mesrob B., Braunitzer G.
    Biochemistry 29:527-534(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE.
    Strain: 76.

Entry informationi

Entry nameiNPRE_BACPU
AccessioniPrimary (citable) accession number: P68734
Secondary accession number(s): P06142, P25268
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 1, 1988
Last sequence update: December 7, 2004
Last modified: January 7, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.