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P68699

- ATPL_ECOLI

UniProt

P68699 - ATPL_ECOLI

Protein

ATP synthase subunit c

Gene

atpE

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    F1F0 ATP synthase produces ATP from ADP in the presence of a proton or sodium gradient. F-type ATPases consist of two structural domains, F1 containing the extramembraneous catalytic core and F0 containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation.
    Key component of the F0 channel; it plays a direct role in translocation across the membrane. A homomeric c-ring of 10 subunits forms the central stalk rotor element with the F1 delta and epsilon subunits.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei61 – 611Reversibly protonated during proton transport

    GO - Molecular functioni

    1. lipid binding Source: UniProtKB-KW
    2. proton-transporting ATPase activity, rotational mechanism Source: EcoCyc
    3. proton-transporting ATP synthase activity, rotational mechanism Source: EcoCyc

    GO - Biological processi

    1. ATP hydrolysis coupled proton transport Source: InterPro
    2. ATP synthesis coupled proton transport Source: InterPro

    Keywords - Biological processi

    ATP synthesis, Hydrogen ion transport, Ion transport, Transport

    Keywords - Ligandi

    Lipid-binding

    Enzyme and pathway databases

    BioCyciEcoCyc:ATPE-MONOMER.
    ECOL316407:JW3715-MONOMER.
    MetaCyc:ATPE-MONOMER.

    Protein family/group databases

    TCDBi3.A.2.1.1. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP synthase subunit c
    Alternative name(s):
    ATP synthase F(0) sector subunit c
    Dicyclohexylcarbodiimide-binding protein
    F-type ATPase subunit c
    Short name:
    F-ATPase subunit c
    Lipid-binding protein
    Gene namesi
    Name:atpE
    Synonyms:papH, uncE
    Ordered Locus Names:b3737, JW3715
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG10102. atpE.

    Subcellular locationi

    Cell inner membrane 1 Publication; Multi-pass membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: EcoliWiki
    2. plasma membrane Source: UniProtKB-SubCell
    3. proton-transporting ATP synthase complex, coupling factor F(o) Source: EcoliWiki

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, CF(0), Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi23 – 231G → D in uncE429; unable to assemble in the membrane. 1 Publication
    Mutagenesisi28 – 281I → V in DC1; has a functional F0 as well as F1 part. However, the ATPase activity is inhibited. 1 Publication
    Mutagenesisi31 – 311L → F in uncE408 and uncE463; unable to assemble in the membrane. 1 Publication
    Mutagenesisi61 – 611D → G in DG 7/1; contains an enzymatically active F1 component, but no functional F0 component. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 7979ATP synthase subunit cPRO_0000112144Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-formylmethionineBy similarity

    Keywords - PTMi

    Formylation

    Proteomic databases

    PaxDbiP68699.
    PRIDEiP68699.

    Expressioni

    Gene expression databases

    GenevestigatoriP68699.

    Interactioni

    Subunit structurei

    F-type ATPases have 2 components, F1 - the catalytic core - and F0 - the membrane proton channel. F1 has five subunits: alpha3, beta3, gamma1, delta1, epsilon1. F0 has three main subunits: a1, b2 and c(10). The alpha and beta chains form an alternating ring which encloses part of the gamma chain. F1 is attached to F0 by a central stalk formed by the gamma and epsilon chains, while a peripheral stalk is formed by the delta and b chains.1 Publication

    Protein-protein interaction databases

    DIPiDIP-2198N.
    IntActiP68699. 3 interactions.
    MINTiMINT-8087956.
    STRINGi511145.b3737.

    Structurei

    Secondary structure

    1
    79
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi2 – 3938
    Beta strandi44 – 463
    Helixi47 – 7832

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1A91NMR-A1-79[»]
    1ATYNMR-A1-79[»]
    1C0VNMR-A1-79[»]
    1C17NMR-A/B/C/D/E/F/G/H/I/J/K/L1-79[»]
    1C99NMR-A1-79[»]
    1IJPNMR-A1-79[»]
    1J7Fmodel-A/B/C/D/E/F/G/H/I/J/K/L1-79[»]
    1L6TNMR-A1-79[»]
    1QO1X-ray3.90K/L/M/N/O/P/Q/R/S/T1-79[»]
    ProteinModelPortaliP68699.
    SMRiP68699. Positions 1-79.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP68699.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1010Periplasmic
    Topological domaini32 – 5221CytoplasmicAdd
    BLAST
    Topological domaini74 – 796Periplasmic

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei11 – 3121HelicalAdd
    BLAST
    Transmembranei53 – 7321HelicalAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ATPase C chain family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0636.
    HOGENOMiHOG000235244.
    KOiK02110.
    OMAiDMLYIAA.
    OrthoDBiEOG68H8G3.

    Family and domain databases

    Gene3Di1.20.20.10. 1 hit.
    HAMAPiMF_01396. ATP_synth_c_bact.
    InterProiIPR000454. ATPase_F0-cplx_csu.
    IPR005953. ATPase_F0-cplx_csu_bac/chlpt.
    IPR020537. ATPase_F0-cplx_csu_DDCD_BS.
    IPR002379. ATPase_proteolipid_c_like_dom.
    [Graphical view]
    PfamiPF00137. ATP-synt_C. 1 hit.
    [Graphical view]
    PRINTSiPR00124. ATPASEC.
    SUPFAMiSSF81333. SSF81333. 1 hit.
    TIGRFAMsiTIGR01260. ATP_synt_c. 1 hit.
    PROSITEiPS00605. ATPASE_C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P68699-1 [UniParc]FASTAAdd to Basket

    « Hide

    MENLNMDLLY MAAAVMMGLA AIGAAIGIGI LGGKFLEGAA RQPDLIPLLR   50
    TQFFIVMGLV DAIPMIAVGL GLYVMFAVA 79
    Length:79
    Mass (Da):8,256
    Last modified:July 21, 1986 - v1
    Checksum:i0F595A69D8AD1F9E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti76 – 761F → S in CAA23591. (PubMed:6266400)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J01594 Genomic DNA. Translation: AAA24732.1.
    V00310 Genomic DNA. Translation: CAA23591.1.
    M25464 Genomic DNA. Translation: AAA83870.1.
    M12214 Genomic DNA. Translation: AAA23668.1.
    V00266 Genomic DNA. Translation: CAA23522.1.
    V00264 Genomic DNA. Translation: CAA23515.1.
    V01506 Genomic DNA. Translation: CAA24752.1.
    X01631 Genomic DNA. Translation: CAA25777.1.
    L10328 Genomic DNA. Translation: AAA62089.1.
    U00096 Genomic DNA. Translation: AAC76760.1.
    AP009048 Genomic DNA. Translation: BAE77551.1.
    PIRiB93732. LWECA.
    RefSeqiNP_418193.1. NC_000913.3.
    YP_491692.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC76760; AAC76760; b3737.
    BAE77551; BAE77551; BAE77551.
    GeneIDi12933605.
    948253.
    KEGGiecj:Y75_p3431.
    eco:b3737.
    PATRICi32122971. VBIEscCol129921_3861.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J01594 Genomic DNA. Translation: AAA24732.1 .
    V00310 Genomic DNA. Translation: CAA23591.1 .
    M25464 Genomic DNA. Translation: AAA83870.1 .
    M12214 Genomic DNA. Translation: AAA23668.1 .
    V00266 Genomic DNA. Translation: CAA23522.1 .
    V00264 Genomic DNA. Translation: CAA23515.1 .
    V01506 Genomic DNA. Translation: CAA24752.1 .
    X01631 Genomic DNA. Translation: CAA25777.1 .
    L10328 Genomic DNA. Translation: AAA62089.1 .
    U00096 Genomic DNA. Translation: AAC76760.1 .
    AP009048 Genomic DNA. Translation: BAE77551.1 .
    PIRi B93732. LWECA.
    RefSeqi NP_418193.1. NC_000913.3.
    YP_491692.1. NC_007779.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1A91 NMR - A 1-79 [» ]
    1ATY NMR - A 1-79 [» ]
    1C0V NMR - A 1-79 [» ]
    1C17 NMR - A/B/C/D/E/F/G/H/I/J/K/L 1-79 [» ]
    1C99 NMR - A 1-79 [» ]
    1IJP NMR - A 1-79 [» ]
    1J7F model - A/B/C/D/E/F/G/H/I/J/K/L 1-79 [» ]
    1L6T NMR - A 1-79 [» ]
    1QO1 X-ray 3.90 K/L/M/N/O/P/Q/R/S/T 1-79 [» ]
    ProteinModelPortali P68699.
    SMRi P68699. Positions 1-79.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-2198N.
    IntActi P68699. 3 interactions.
    MINTi MINT-8087956.
    STRINGi 511145.b3737.

    Protein family/group databases

    TCDBi 3.A.2.1.1. the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.

    Proteomic databases

    PaxDbi P68699.
    PRIDEi P68699.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC76760 ; AAC76760 ; b3737 .
    BAE77551 ; BAE77551 ; BAE77551 .
    GeneIDi 12933605.
    948253.
    KEGGi ecj:Y75_p3431.
    eco:b3737.
    PATRICi 32122971. VBIEscCol129921_3861.

    Organism-specific databases

    EchoBASEi EB0100.
    EcoGenei EG10102. atpE.

    Phylogenomic databases

    eggNOGi COG0636.
    HOGENOMi HOG000235244.
    KOi K02110.
    OMAi DMLYIAA.
    OrthoDBi EOG68H8G3.

    Enzyme and pathway databases

    BioCyci EcoCyc:ATPE-MONOMER.
    ECOL316407:JW3715-MONOMER.
    MetaCyc:ATPE-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P68699.
    PROi P68699.

    Gene expression databases

    Genevestigatori P68699.

    Family and domain databases

    Gene3Di 1.20.20.10. 1 hit.
    HAMAPi MF_01396. ATP_synth_c_bact.
    InterProi IPR000454. ATPase_F0-cplx_csu.
    IPR005953. ATPase_F0-cplx_csu_bac/chlpt.
    IPR020537. ATPase_F0-cplx_csu_DDCD_BS.
    IPR002379. ATPase_proteolipid_c_like_dom.
    [Graphical view ]
    Pfami PF00137. ATP-synt_C. 1 hit.
    [Graphical view ]
    PRINTSi PR00124. ATPASEC.
    SUPFAMi SSF81333. SSF81333. 1 hit.
    TIGRFAMsi TIGR01260. ATP_synt_c. 1 hit.
    PROSITEi PS00605. ATPASE_C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Amino acid sequence of the ATPase proteolipid from mitochondria, chloroplasts and bacteria (wild type and mutants)."
      Sebald W., Hoppe J., Wachter E.
      (In) Quagliariello E., Palmieri F., Papa S., Klingenberg M. (eds.); Function and molecular aspects of biomembrane transport, pp.63-74, Elsevier, Amsterdam (1979)
      Cited for: PROTEIN SEQUENCE.
    2. "Nucleotide sequence of genes coding for dicyclohexylcarbodiimide-binding protein and the alpha subunit of proton-translocating ATPase of Escherichia coli."
      Kanazawa H., Mabuchi K., Kayano T., Tamura F., Futai M.
      Biochem. Biophys. Res. Commun. 100:219-225(1981) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The nucleotide sequence of the atp genes coding for the F0 subunits a, b, c and the F1 subunit delta of the membrane bound ATP synthase of Escherichia coli."
      Nielsen J., Hansen F.G., Hoppe J., Friedl P., von Meyenburg K.
      Mol. Gen. Genet. 184:33-39(1981) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "The atp operon: nucleotide sequence of the promoter and the genes for the membrane proteins, and the delta subunit of Escherichia coli ATP-synthase."
      Gay N.J., Walker J.E.
      Nucleic Acids Res. 9:3919-3926(1981) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    5. "The F1F0-ATPase of Escherichia coli. Substitution of proline by leucine at position 64 in the c-subunit causes loss of oxidative phosphorylation."
      Fimmel A.L., Jans D.A., Langman L., James L.B., Ash G.R., Downie J.A., Senior A.E., Gibson F., Cox G.B.
      Biochem. J. 213:451-458(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    6. "DNA sequence around the Escherichia coli unc operon. Completion of the sequence of a 17 kilobase segment containing asnA, oriC, unc, glmS and phoS."
      Walker J.E., Gay N.J., Saraste M., Eberle A.N.
      Biochem. J. 224:799-815(1984) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    7. "Amino acid replacement in dicyclohexylcarbodiimide-reactive proteins from mutant strains of Escherichia coli defective in the energy-transducing ATPase complex."
      Wachter E., Schmid R., Deckers G., Altendorf K.
      FEBS Lett. 113:265-270(1980) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE, MUTAGENESIS OF ILE-28 AND ASP-61.
      Strain: K12.
    8. "Mutations in the uncE gene affecting assembly of the c-subunit of the adenosine triphosphatase of Escherichia coli."
      Jans D.A., Fimmel A.L., Langman L., James L.B., Downie J.A., Senior A.E., Ash G.R., Gibson F., Cox G.B.
      Biochem. J. 211:717-726(1983) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLY-23 AND LEU-31.
    9. "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication."
      Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.
      Genomics 16:551-561(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    10. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    11. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    12. "The preferred stoichiometry of c subunits in the rotary motor sector of Escherichia coli ATP synthase is 10."
      Jiang W., Hermolin J., Fillingame R.H.
      Proc. Natl. Acad. Sci. U.S.A. 98:4966-4971(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBUNIT, SUBUNIT C FUSIONS.
    13. "Global topology analysis of the Escherichia coli inner membrane proteome."
      Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
      Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION.
      Strain: K12 / MG1655 / ATCC 47076.
    14. "Heteronuclear 1H-15N nuclear magnetic resonance studies of the c subunit of the Escherichia coli F1F0 ATP synthase: assignment and secondary structure."
      Norwood T.J., Crawford D.A., Steventon M.E., Driscoll P.C., Campbell I.D.
      Biochemistry 31:6285-6290(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR, SITE.
    15. "Determination of local protein structure by spin label difference 2D NMR: the region neighboring Asp61 of subunit c of the F1F0 ATP synthase."
      Girvin M.E., Fillingame R.H.
      Biochemistry 34:1635-1645(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.
    16. "Solution structure of the transmembrane H+-transporting subunit c of the F1F0 ATP synthase."
      Girvin M.E., Rastogi V.K., Abildgaard F., Markley J.L., Fillingame R.H.
      Biochemistry 37:8817-8824(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.
    17. "Structural changes linked to proton translocation by subunit c of the ATP synthase."
      Rastogi V.K., Girvin M.E.
      Nature 402:263-268(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: STRUCTURE BY NMR.
    18. "Model of the c-subunit oligomer in the membrane domain of F-ATPases."
      Groth G., Walker J.E.
      FEBS Lett. 410:117-123(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING.

    Entry informationi

    Entry nameiATPL_ECOLI
    AccessioniPrimary (citable) accession number: P68699
    Secondary accession number(s): P00844, Q2M855
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: July 21, 1986
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Dicyclohexylcarbodiimide (DCDD) binding to the reversibly protonated aspartate residue inhibits ATPase in vitro.
    In this organism c-rings of between c8 and c(12) can be isolated in vivo following experimental manipulations, however only c8 and c9, in addition to c(10), are partially functional.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3