Reviewed, UniProtKB/Swiss-Prot P68698 (TOP1_VACCW)

Names and origin

Protein names

Recommended name:
    DNA topoisomerase 1
    EC=5.99.1.2
Alternative name(s):
    DNA topoisomerase I
    Late protein H7

Gene names

Name:	TOP1
Ordered Locus Names:	VACWR104
ORF Names:	H7

Organism

Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))

Taxonomic identifier

10254 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus

Virus host

Bos taurus (Bovine) [TaxID: 9913]

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another.
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
Miscellaneous	When a topoisomerase transiently breaks a DNA backbone bond, it simultaneously forms a protein-DNA link, in which a tyrosyl oxygen in the enzyme is joined to a DNA phosphorus at one end of the enzyme-severed DNA strand.
Sequence similarities	Belongs to the eukaryotic type I topoisomerase family.

Ontologies

Keywords
Developmental stage	Late protein
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro DNA unwinding during replication Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 314

314

DNA topoisomerase 1

PRO_0000145216

Sites

Active site

274

1

O-(3'-phospho-DNA)-tyrosine intermediate By similarity

Experimental info

Mutagenesis

216

1

G → E or K: No change in activity.

Mutagenesis

217

1

I → P: Temperature-sensitive for DNA relaxation in vitro.

Mutagenesis

220

1

K → D: Loss of activity.

Mutagenesis

220

1

K → I or N: Reduced activity.

Mutagenesis

223

1

R → E or G: Loss of activity.

Mutagenesis

223

1

R → K: Reduced activity.

Mutagenesis

224

1

T → G or P: Reduced activity.

Mutagenesis

225

1

Y → H: No change in activity.

Mutagenesis

225

1

Y → R or S: Reduced activity.

Secondary structure

1

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314

Helix Strand Turn

Details...

Beta strand

3 – 7

5

Beta strand

10 – 14

5

Helix

27 – 33

7

Beta strand

41 – 46

6

Helix

50 – 53

4

Beta strand

56 – 62

7

Beta strand

68 – 72

5

Helix

82 – 105

24

Turn

109 – 111

3

Helix

115 – 125

11

Helix

140 – 146

7

Helix

150 – 152

3

Beta strand

153 – 156

4

Beta strand

159 – 162

4

Beta strand

164 – 166

3

Turn

167 – 169

3

Beta strand

170 – 172

3

Beta strand

175 – 177

3

Beta strand

180 – 182

3

Helix

183 – 189

7

Beta strand

197 – 200

4

Helix

205 – 214

10

Helix

219 – 241

23

Beta strand

242 – 244

3

Helix

248 – 263

16

Helix

269 – 282

14

Helix

288 – 292

5

Helix

297 – 309

13

Sequences

	Sequence		Length	Mass (Da)
	P68698-1 [UniParc]. Last modified August 1, 1988. Version 1. Checksum: E76FBCFCBF259D27	FASTA	314	36,666
	10 20 30 40 50 60 MRALFYKDGK LFTDNNFLNP VSDDNPAYEV LQHVKIPTHL TDVVVYEQTW EEALTRLIFV 70 80 90 100 110 120 GSDSKGRRQY FYGKMHVQNR NAKRDRIFVR VYNVMKRINC FINKNIKKSS TDSNYQLAVF 130 140 150 160 170 180 MLMETMFFIR FGKMKYLKEN ETVGLLTLKN KHIEISPDEI VIKFVGKDKV SHEFVVHKSN 190 200 210 220 230 240 RLYKPLLKLT DDSSPEEFLF NKLSERKVYE CIKQFGIRIK DLRTYGVNYT FLYNFWTNVK 250 260 270 280 290 300 SISPLPSPKK LIALTIKQTA EVVGHTPSIS KRAYMATTIL EMVKDKNFLD VVSKTTFDEF 310 LSIVVDHVKS STDG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Conserved TAAATG sequence at the transcriptional and translational initiation sites of vaccinia virus late genes deduced by structural and functional analysis of the HindIII H genome fragment." Rosel J.L., Earl P.L., Weir J.P., Moss B. J. Virol. 60:436-449(1986) [PubMed: 3021979] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Biochemical analysis of mutant alleles of the vaccinia virus topoisomerase I carrying targeted substitutions in a highly conserved domain." Klemperer N., Traktman P. J. Biol. Chem. 268:15887-15899(1993) [PubMed: 8393454] [Abstract] Cited for: NUCLEOTIDE SEQUENCE, MUTAGENESIS.
[3]	"Sequencing of the coding region of Vaccinia-WR to an average 9-fold redundancy and an error rate of 0.16/10kb." Esposito J.J., Frace A.M., Sammons S.A., Olsen-Rasmussen M., Osborne J., Wohlhueter R. Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"Identification of a vaccinia virus gene encoding a type I DNA topoisomerase." Shuman S., Moss B. Proc. Natl. Acad. Sci. U.S.A. 84:7478-7482(1987) [PubMed: 2823264] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-22, IDENTIFICATION.
[5]	"Crystal structure of the amino-terminal fragment of vaccinia virus DNA topoisomerase I at 1.6-A resolution." Sharma A., Hanai R., Mondragon A. Structure 2:767-777(1994) [PubMed: 7994576] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 1-77.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M13209 Genomic DNA. Translation: AAB59842.1.
L13447 Unassigned DNA. Translation: AAA02841.1.
AY243312 Genomic DNA. Translation: AAO89383.1.

PIR

QQVZH7. G24481.

RefSeq

YP_232986.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1A41	X-ray	2.30	A	81-314	[»]
1VCC	X-ray	1.60	A	1-77	[»]

SMR

P68698. Positions 1-312.

ModBase

Search...

Genome annotation databases

GeneID

3707560.

Family and domain databases

InterPro

IPR018521. TopoI_AS.
IPR001631. TopoI_C.
IPR014711. TopoI_cat_a-hlx-sub_euk.
IPR013500. TopoI_cat_euk.
IPR015346. TopoI_N_vir.
[Graphical view]

Gene3D

G3DSA:3.90.15.10. TopoI_cat_a-hlx-sub_euk. 1 hit.

Pfam

PF01028. Topoisom_I. 1 hit.
PF09266. VirDNA-topo-I_N. 1 hit.
[Graphical view]

PRINTS

PR00416. EUTPISMRASEI.

PROSITE

PS00176. TOPOISOMERASE_I_EUK. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

TOP1_VACCW

Accession

Primary (citable) accession number: P68698
Secondary accession number(s): P08585

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1988
Last sequence update:	August 1, 1988
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)