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P68697

- TOP1_VACCC

UniProt

P68697 - TOP1_VACCC

Protein

DNA topoisomerase 1B

Gene

TOP1

Organism
Vaccinia virus (strain Copenhagen) (VACV)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 51 (01 Oct 2014)
      Sequence version 1 (01 Aug 1988)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity.By similarity

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei168 – 1681Involved in religationBy similarity
    Active sitei274 – 2741O-(3'-phospho-DNA)-tyrosine intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-EC
    3. DNA topoisomerase type II (ATP-hydrolyzing) activity Source: InterPro

    GO - Biological processi

    1. DNA topological change Source: InterPro

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 1B (EC:5.99.1.2)
    Short name:
    TopIB
    Alternative name(s):
    DNA topoisomerase I
    Late protein H6
    Gene namesi
    Name:TOP1
    ORF Names:H6R
    OrganismiVaccinia virus (strain Copenhagen) (VACV)
    Taxonomic identifieri10249 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
    Virus hostiHomo sapiens (Human) [TaxID: 9606]
    ProteomesiUP000008269: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 314314DNA topoisomerase 1BPRO_0000145215Add
    BLAST

    Expressioni

    Inductioni

    Expressed in the late phase of the viral replicative cycle.

    Keywords - Developmental stagei

    Late protein

    Structurei

    3D structure databases

    ProteinModelPortaliP68697.
    SMRiP68697. Positions 1-314.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the type IB topoisomerase family.Curated

    Family and domain databases

    Gene3Di1.20.120.380. 1 hit.
    3.30.66.10. 1 hit.
    3.90.15.10. 1 hit.
    InterProiIPR011010. DNA_brk_join_enz.
    IPR001631. TopoI.
    IPR018521. TopoI_AS.
    IPR014711. TopoI_cat_a-hlx-sub_euk.
    IPR027362. TopoI_cat_dom2.
    IPR013500. TopoI_cat_euk.
    IPR015346. TopoI_N_vir.
    [Graphical view]
    PfamiPF01028. Topoisom_I. 1 hit.
    PF09266. VirDNA-topo-I_N. 1 hit.
    [Graphical view]
    PRINTSiPR00416. EUTPISMRASEI.
    SUPFAMiSSF56349. SSF56349. 1 hit.
    PROSITEiPS00176. TOPOISOMERASE_I_EUK. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P68697-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRALFYKDGK LFTDNNFLNP VSDDNPAYEV LQHVKIPTHL TDVVVYEQTW    50
    EEALTRLIFV GSDSKGRRQY FYGKMHVQNR NAKRDRIFVR VYNVMKRINC 100
    FINKNIKKSS TDSNYQLAVF MLMETMFFIR FGKMKYLKEN ETVGLLTLKN 150
    KHIEISPDEI VIKFVGKDKV SHEFVVHKSN RLYKPLLKLT DDSSPEEFLF 200
    NKLSERKVYE CIKQFGIRIK DLRTYGVNYT FLYNFWTNVK SISPLPSPKK 250
    LIALTIKQTA EVVGHTPSIS KRAYMATTIL EMVKDKNFLD VVSKTTFDEF 300
    LSIVVDHVKS STDG 314
    Length:314
    Mass (Da):36,666
    Last modified:August 1, 1988 - v1
    Checksum:iE76FBCFCBF259D27
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M35027 Genomic DNA. Translation: AAA48093.1.
    PIRiG24481. QQVZH7.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M35027 Genomic DNA. Translation: AAA48093.1 .
    PIRi G24481. QQVZH7.

    3D structure databases

    ProteinModelPortali P68697.
    SMRi P68697. Positions 1-314.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.20.120.380. 1 hit.
    3.30.66.10. 1 hit.
    3.90.15.10. 1 hit.
    InterProi IPR011010. DNA_brk_join_enz.
    IPR001631. TopoI.
    IPR018521. TopoI_AS.
    IPR014711. TopoI_cat_a-hlx-sub_euk.
    IPR027362. TopoI_cat_dom2.
    IPR013500. TopoI_cat_euk.
    IPR015346. TopoI_N_vir.
    [Graphical view ]
    Pfami PF01028. Topoisom_I. 1 hit.
    PF09266. VirDNA-topo-I_N. 1 hit.
    [Graphical view ]
    PRINTSi PR00416. EUTPISMRASEI.
    SUPFAMi SSF56349. SSF56349. 1 hit.
    PROSITEi PS00176. TOPOISOMERASE_I_EUK. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    2. "Appendix to 'The complete DNA sequence of vaccinia virus'."
      Goebel S.J., Johnson G.P., Perkus M.E., Davis S.W., Winslow J.P., Paoletti E.
      Virology 179:517-563(1990)
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

    Entry informationi

    Entry nameiTOP1_VACCC
    AccessioniPrimary (citable) accession number: P68697
    Secondary accession number(s): P08585
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1988
    Last sequence update: August 1, 1988
    Last modified: October 1, 2014
    This is version 51 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3