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Protein

Profilin-1A

Gene
N/A
Organism
Acanthamoeba castellanii (Amoeba)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds to actin and affects the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin, whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei116Actin1

GO - Molecular functioni

  • actin monomer binding Source: UniProtKB

GO - Biological processi

  • actin cytoskeleton organization Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Profilin-1A
Alternative name(s):
Acidic profilin IA
Profilin IA
OrganismiAcanthamoeba castellanii (Amoeba)
Taxonomic identifieri5755 [NCBI]
Taxonomic lineageiEukaryotaAmoebozoaDiscoseaLongamoebiaCentramoebidaAcanthamoebidaeAcanthamoeba

Subcellular locationi

GO - Cellular componenti

  • actin cytoskeleton Source: UniProtKB
  • cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001995832 – 126Profilin-1AAdd BLAST125

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei104N6,N6,N6-trimethyllysine1 Publication1

Keywords - PTMi

Methylation

Interactioni

Subunit structurei

Occurs in many kinds of cells as a complex with monomeric actin in a 1:1 ratio.

GO - Molecular functioni

  • actin monomer binding Source: UniProtKB

Structurei

Secondary structure

1126
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi3 – 8Combined sources6
Turni9 – 11Combined sources3
Helixi12 – 14Combined sources3
Beta strandi18 – 24Combined sources7
Beta strandi29 – 32Combined sources4
Helixi40 – 49Combined sources10
Helixi54 – 57Combined sources4
Beta strandi61 – 63Combined sources3
Beta strandi66 – 73Combined sources8
Beta strandi75 – 82Combined sources8
Beta strandi85 – 91Combined sources7
Beta strandi93 – 101Combined sources9
Helixi107 – 123Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PRQX-ray2.50A2-126[»]
2PRFNMR-A2-126[»]
ProteinModelPortaliP68696.
SMRiP68696.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP68696.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 36Actin-bindingSequence analysisAdd BLAST35

Sequence similaritiesi

Belongs to the profilin family.Curated

Family and domain databases

CDDicd00148. PROF. 1 hit.
InterProiIPR005455. PFN.
IPR027310. Profilin_CS.
[Graphical view]
PANTHERiPTHR11604. PTHR11604. 1 hit.
PfamiPF00235. Profilin. 1 hit.
[Graphical view]
PRINTSiPR00392. PROFILIN.
PR01640. PROFILINPLNT.
SMARTiSM00392. PROF. 1 hit.
[Graphical view]
SUPFAMiSSF55770. SSF55770. 1 hit.
PROSITEiPS00414. PROFILIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P68696-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSWQTYVDTN LVGTGAVTQA AILGLDGNTW ATSAGFAVTP AQGQTLASAF
60 70 80 90 100
NNADPIRASG FDLAGVHYVT LRADDRSIYG KKGSAGVITV KTSKSILVGV
110 120
YNEKIQPGTA ANVVEKLADY LIGQGF
Length:126
Mass (Da):13,084
Last modified:January 23, 2007 - v2
Checksum:i69EA51991FE3D58B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2S → T AA sequence (PubMed:3881427).Curated1
Sequence conflicti5T → S AA sequence (PubMed:3881427).Curated1
Sequence conflicti32 – 33TS → SF AA sequence (PubMed:3881427).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27485 mRNA. Translation: AAA27710.1.
PIRiB48405.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L27485 mRNA. Translation: AAA27710.1.
PIRiB48405.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PRQX-ray2.50A2-126[»]
2PRFNMR-A2-126[»]
ProteinModelPortaliP68696.
SMRiP68696.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP68696.

Family and domain databases

CDDicd00148. PROF. 1 hit.
InterProiIPR005455. PFN.
IPR027310. Profilin_CS.
[Graphical view]
PANTHERiPTHR11604. PTHR11604. 1 hit.
PfamiPF00235. Profilin. 1 hit.
[Graphical view]
PRINTSiPR00392. PROFILIN.
PR01640. PROFILINPLNT.
SMARTiSM00392. PROF. 1 hit.
[Graphical view]
SUPFAMiSSF55770. SSF55770. 1 hit.
PROSITEiPS00414. PROFILIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPRO1A_ACACA
AccessioniPrimary (citable) accession number: P68696
Secondary accession number(s): P07763
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1988
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 62 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.