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Protein

Thymidylate kinase

Gene

TMK

Organism
Vaccinia virus (strain Copenhagen) (VACV)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Poxvirus TMP kinase is able to phosphorylate dTMP, dUMP and also dGMP from any purine and pyrimidine nucleoside triphosphate. The large substrate specificity is explained by the presence of a canal connecting the edge of the dimer interface to the TMP base binding pocket, canal not found in the human homolog (By similarity).By similarity

Catalytic activityi

ATP + dTMP = ADP + dTDP.

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 18ATPCurated8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Nucleotide biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.4.9. 6591.
UniPathwayiUPA00575.

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate kinase (EC:2.7.4.9)
Alternative name(s):
dTMP kinase
Gene namesi
Name:TMK
ORF Names:A48R
OrganismiVaccinia virus (strain Copenhagen) (VACV)
Taxonomic identifieri10249 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000008269 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001552171 – 204Thymidylate kinaseAdd BLAST204

Interactioni

Subunit structurei

Homodimer; the dimer arrangement is orthogonal and not antiparallel as in human enzyme.By similarity

Protein-protein interaction databases

DIPiDIP-48617N.

Structurei

Secondary structure

1204
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 10Combined sources5
Helixi17 – 26Combined sources10
Helixi30 – 32Combined sources3
Beta strandi33 – 39Combined sources7
Helixi44 – 53Combined sources10
Helixi61 – 73Combined sources13
Helixi76 – 84Combined sources9
Beta strandi88 – 93Combined sources6
Helixi95 – 104Combined sources10
Helixi109 – 116Combined sources8
Beta strandi119 – 121Combined sources3
Beta strandi123 – 128Combined sources6
Helixi132 – 135Combined sources4
Beta strandi140 – 142Combined sources3
Helixi148 – 161Combined sources14
Beta strandi168 – 171Combined sources4
Helixi177 – 194Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2V54X-ray2.40A/B1-204[»]
2W0SX-ray2.92A/B1-204[»]
ProteinModelPortaliP68693.
SMRiP68693.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP68693.

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate kinase family.Curated

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR018095. Thymidylate_kin_CS.
IPR018094. Thymidylate_kinase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00041. DTMP_kinase. 1 hit.
PROSITEiPS01331. THYMIDYLATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P68693-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRGALIVFE GLDKSGKTTQ CMNIMESIPA NTIKYLNFPQ RSTVTGKMID
60 70 80 90 100
DYLTRKKTYN DHIVNLLFCA NRWEFASFIQ EQLEQGITLI VDRYAFSGVA
110 120 130 140 150
YAAAKGASMT LSKSYESGLP KPDLVIFLES GSKEINRNVG EEIYEDVTFQ
160 170 180 190 200
QKVLQEYKKM IEEGDIHWQI ISSEFEEDVK KELIKNIVIE AIHTVTGPVG

QLWM
Length:204
Mass (Da):23,219
Last modified:December 7, 2004 - v1
Checksum:i911DFD2C671CF6B3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35027 Genomic DNA. Translation: AAA48180.1.
PIRiE42522. KIVZ5W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M35027 Genomic DNA. Translation: AAA48180.1.
PIRiE42522. KIVZ5W.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2V54X-ray2.40A/B1-204[»]
2W0SX-ray2.92A/B1-204[»]
ProteinModelPortaliP68693.
SMRiP68693.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-48617N.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00575.
BRENDAi2.7.4.9. 6591.

Miscellaneous databases

EvolutionaryTraceiP68693.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR018095. Thymidylate_kin_CS.
IPR018094. Thymidylate_kinase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00041. DTMP_kinase. 1 hit.
PROSITEiPS01331. THYMIDYLATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKTHY_VACCC
AccessioniPrimary (citable) accession number: P68693
Secondary accession number(s): P13410
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: December 7, 2004
Last modified: November 2, 2016
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.