30S ribosomal protein S21 - Escherichia coli (strain K12)

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P68679 (RS21_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 86. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
30S ribosomal protein S21

Gene names

Name:	rpsU
Ordered Locus Names:	b3065, JW3037

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	71 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities	Belongs to the ribosomal protein S21P family.
Mass spectrometry	Molecular mass is 8368.8 Da from positions 2 - 71. Determined by MALDI. Ref.7

Ontologies

Keywords
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	translation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytosolic small ribosomal subunit Inferred from direct assay PubMed 4587209. Source: EcoliWiki
Molecular_function	structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed Ref.5

Chain

2 – 71

30S ribosomal protein S21 HAMAP-Rule MF_00358

PRO_0000178332

Secondary structure

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P68679 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: B2E8A18BFBB2F685
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FASTA

8,500

        10         20         30         40         50         60 
MPVIKVRENE PFDVALRRFK RSCEKAGVLA EVRRREFYEK PTTERKRAKA SAVKRHAKKL 

        70 
ARENARRTRL Y

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The operon that encodes the sigma subunit of RNA polymerase also encodes ribosomal protein S21 and DNA primase in E. coli K12." Burton Z.F., Gross C.A., Watanabe K.K., Burgess R.R. Cell 32:335-349(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Regulation of the rpsU-dnaG-rpoD macromolecular synthesis operon and the initiation of DNA replication in Escherichia coli K-12." Lupski J.R., Smiley B.L., Godson G.N. Mol. Gen. Genet. 189:48-57(1983) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Determination of the complete amino acid sequence of protein S21 from Escherichia coli ribosomes." Vandekerckhove J., Rombauts W., Peeters B., Wittmann-Liebold B. Hoppe-Seyler's Z. Physiol. Chem. 356:1955-1976(1975) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-71. Strain: K.
[6]	"Protein-rRNA binding features and their structural and functional implications in ribosomes as determined by cross-linking studies." Urlaub H., Kruft V., Bischof O., Mueller E.-C., Wittmann-Liebold B. EMBO J. 14:4578-4588(1995) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 26-35, CROSS-LINKING TO RRNA. Strain: MRE-600.
[7]	"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry." Arnold R.J., Reilly J.P. Anal. Biochem. 269:105-112(1999) [PubMed] [Europe PMC] [Abstract] Cited for: MASS SPECTROMETRY. Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
[8]	"Structures of the bacterial ribosome at 3.5 A resolution." Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D. Science 310:827-834(2005) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES. Strain: MRE-600.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

J01687 Genomic DNA. Translation: AAA24599.1.
V00346 Genomic DNA. Translation: CAA23635.1.
U28379 Genomic DNA. Translation: AAA89145.1.
U00096 Genomic DNA. Translation: AAC76101.1.
AP009048 Genomic DNA. Translation: BAE77116.1.

PIR

R3EC21. A02749.

RefSeq

NP_417537.1. NC_000913.2.
YP_491257.1. NC_007779.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1VS5	X-ray	3.46	U	1-71	[»]
1VS7	X-ray	3.46	U	1-71	[»]
2AVY	X-ray	3.46	U	1-71	[»]
2AW7	X-ray	3.46	U	1-71	[»]
2I2P	X-ray	3.22	U	2-70	[»]
2I2U	X-ray	3.22	U	2-70	[»]
2QAL	X-ray	3.21	U	2-71	[»]
2QAN	X-ray	3.21	U	2-71	[»]
2QB9	X-ray	3.54	U	2-71	[»]
2QBB	X-ray	3.54	U	2-71	[»]
2QBD	X-ray	3.30	U	2-71	[»]
2QBF	X-ray	3.30	U	2-71	[»]
2QBH	X-ray	4.00	U	2-71	[»]
2QBJ	X-ray	4.00	U	2-71	[»]
2QOU	X-ray	3.93	U	2-71	[»]
2QOW	X-ray	3.93	U	2-71	[»]
2QOY	X-ray	3.50	U	2-71	[»]
2QP0	X-ray	3.50	U	2-71	[»]
2VHO	X-ray	3.74	U	1-71	[»]
2VHP	X-ray	3.74	U	1-71	[»]
2WWL	electron microscopy	5.80	U	4-54	[»]
2YKR	electron microscopy	9.80	U	4-54	[»]
2Z4K	X-ray	4.45	U	2-71	[»]
2Z4M	X-ray	4.45	U	2-71	[»]
3DF1	X-ray	3.50	U	1-71	[»]
3DF3	X-ray	3.50	U	1-71	[»]
3E1A	electron microscopy	-	Q	1-71	[»]
3E1C	electron microscopy	-	Q	1-71	[»]
3FIH	electron microscopy	6.70	U	4-54	[»]
3I1M	X-ray	3.19	U	1-71	[»]
3I1O	X-ray	3.19	U	1-71	[»]
3I1Q	X-ray	3.81	U	1-71	[»]
3I1S	X-ray	3.81	U	1-71	[»]
3I1Z	X-ray	3.71	U	1-71	[»]
3I21	X-ray	3.71	U	1-71	[»]
3IZV	electron microscopy	-	Y	1-71	[»]
3IZW	electron microscopy	-	Y	1-71	[»]
3J00	electron microscopy	-	U	2-71	[»]
3J0U	electron microscopy	12.10	X	2-71	[»]
3J0V	electron microscopy	14.70	X	2-71	[»]
3J0X	electron microscopy	13.50	X	2-71	[»]
3J0Z	electron microscopy	11.50	X	2-71	[»]
3J10	electron microscopy	11.50	X	2-71	[»]
3J13	electron microscopy	13.10	W	2-71	[»]
3J18	electron microscopy	8.30	U	4-54	[»]
3KC4	electron microscopy	-	U	1-71	[»]
3OAQ	X-ray	3.25	U	4-54	[»]
3OAR	X-ray	3.25	U	4-54	[»]
3OFA	X-ray	3.19	U	4-54	[»]
3OFB	X-ray	3.19	U	4-54	[»]
3OFO	X-ray	3.10	U	4-54	[»]
3OFP	X-ray	3.10	U	4-54	[»]
3OFX	X-ray	3.29	U	4-54	[»]
3OFY	X-ray	3.29	U	4-54	[»]
3OR9	X-ray	3.30	U	1-71	[»]
3ORA	X-ray	3.30	U	1-71	[»]
3SFS	X-ray	3.20	U	1-71	[»]
3UOQ	X-ray	3.70	U	1-71	[»]
4A2I	electron microscopy	16.50	U	4-54	[»]
4ADV	electron microscopy	13.50	U	1-71	[»]
4GAQ	X-ray	3.30	U	1-71	[»]
4GAS	X-ray	3.30	U	1-71	[»]
4GD1	X-ray	3.00	U	4-54	[»]
4GD2	X-ray	3.00	U	4-54	[»]

ProteinModelPortal

P68679.

SMR

P68679. Positions 2-71.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-47839N.

IntAct

P68679. 22 interactions.

MINT

MINT-1281887.

STRING

511145.b3065.

Proteomic databases

PaxDb

P68679.

PRIDE

P68679.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC76101; AAC76101; b3065.
BAE77116; BAE77116; BAE77116.

GeneID

12933220.
947577.

KEGG

ecj:Y75_p2991.
eco:b3065.

PATRIC

32121544. VBIEscCol129921_3159.

Organism-specific databases

EchoBASE

EB0913.

EcoGene

EG10920. rpsU.

Phylogenomic databases

eggNOG

COG0828.

HOGENOM

HOG000157460.

K02970.

OMA

PFERKPP.

ProtClustDB

PRK00270.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10920-MONOMER.
ECOL316407:JW3037-MONOMER.

Gene expression databases

Genevestigator

P68679.

Family and domain databases

HAMAP

MF_00358. Ribosomal_S21.

InterPro

IPR001911. Ribosomal_S21.
IPR018278. Ribosomal_S21_CS.
[Graphical view]

Pfam

PF01165. Ribosomal_S21. 1 hit.
[Graphical view]

PRINTS

PR00976. RIBOSOMALS21.

ProDom

PD005521. Ribosomal_S21. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR00030. S21p. 1 hit.

PROSITE

PS01181. RIBOSOMAL_S21. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P68679.

Entry information

Entry name

RS21_ECOLI

Accession

Primary (citable) accession number: P68679
Secondary accession number(s): P02379, Q2M9E0, Q8ZI69

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Ribosomal proteins

Ribosomal proteins families and list of entries

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents