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Reviewed, UniProtKB/Swiss-Prot P68679 (RS21_ECOLI)

Last modified June 16, 2009. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    30S ribosomal protein S21
Gene names
Name: rpsU
Ordered Locus Names: b3065, JW3037
OrganismEscherichia coli (strain K12) [Complete proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length71 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Sequence similarities

Belongs to the ribosomal protein S21P family.

Mass spectrometry

Molecular mass is 8368.8 Da from positions 2 - 71. Determined by MALDI. Ref.7

Ontologies

Keywords
   Molecular functionRibonucleoprotein
Ribosomal protein
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological processtranslation

Inferred from electronic annotation. Source: HAMAP

   Cellular componentribosome

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionstructural constituent of ribosome

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.5
Chain2 – 717030S ribosomal protein S21 HAMAP MF_00358
PRO_0000178332

Secondary structure

......... 71
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P68679-1 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: B2E8A18BFBB2F685

FASTA718,500
        10         20         30         40         50         60 
MPVIKVRENE PFDVALRRFK RSCEKAGVLA EVRRREFYEK PTTERKRAKA SAVKRHAKKL 

        70 
ARENARRTRL Y 

« Hide

References

« Hide 'large scale' references
[1]"The operon that encodes the sigma subunit of RNA polymerase also encodes ribosomal protein S21 and DNA primase in E. coli K12."
Burton Z.F., Gross C.A., Watanabe K.K., Burgess R.R.
Cell 32:335-349(1983) [PubMed: 6186393] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[2]"Regulation of the rpsU-dnaG-rpoD macromolecular synthesis operon and the initiation of DNA replication in Escherichia coli K-12."
Lupski J.R., Smiley B.L., Godson G.N.
Mol. Gen. Genet. 189:48-57(1983) [PubMed: 6222240] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: K12.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Determination of the complete amino acid sequence of protein S21 from Escherichia coli ribosomes."
Vandekerckhove J., Rombauts W., Peeters B., Wittmann-Liebold B.
Hoppe-Seyler's Z. Physiol. Chem. 356:1955-1976(1975) [PubMed: 765257] [Abstract]
Cited for: PROTEIN SEQUENCE OF 2-71.
Strain: K.
[6]"Protein-rRNA binding features and their structural and functional implications in ribosomes as determined by cross-linking studies."
Urlaub H., Kruft V., Bischof O., Mueller E.-C., Wittmann-Liebold B.
EMBO J. 14:4578-4588(1995) [PubMed: 7556101] [Abstract]
Cited for: PROTEIN SEQUENCE OF 26-35, CROSS-LINKING TO RRNA.
Strain: MRE-600.
[7]"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry."
Arnold R.J., Reilly J.P.
Anal. Biochem. 269:105-112(1999) [PubMed: 10094780] [Abstract]
Cited for: MASS SPECTROMETRY.
Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
[8]"Structures of the bacterial ribosome at 3.5 A resolution."
Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D.
Science 310:827-834(2005) [PubMed: 16272117] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES.
Strain: MRE-600.
+Additional computationally mapped references.

Cross-references

Sequence databases

J01687 Genomic DNA. Translation: AAA24599.1.
V00346 Genomic DNA. Translation: CAA23635.1.
U28379 Genomic DNA. Translation: AAA89145.1.
U00096 Genomic DNA. Translation: AAC76101.1.
AP009048 Genomic DNA. Translation: BAE77116.1.
PIRR3EC21. A02749.
RefSeqAP_003615.1.
NP_417537.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1VS5X-ray3.46U1-71[»]
1VS7X-ray3.46U1-71[»]
2AVYX-ray3.46U1-71[»]
2AW7X-ray3.46U1-71[»]
2I2PX-ray3.22U2-70[»]
2I2UX-ray3.22U2-70[»]
2QALX-ray3.21U2-71[»]
2QANX-ray3.21U2-71[»]
2QB9X-ray3.54U2-71[»]
2QBBX-ray3.54U2-71[»]
2QBDX-ray3.30U2-71[»]
2QBFX-ray3.30U2-71[»]
2QBHX-ray4.00U2-71[»]
2QBJX-ray4.00U2-71[»]
2QOUX-ray3.93U2-71[»]
2QOWX-ray3.93U2-71[»]
2QOYX-ray3.50U2-71[»]
2QP0X-ray3.50U2-71[»]
2VHOX-ray3.74U1-71[»]
2VHPX-ray3.74U1-71[»]
2Z4KX-ray4.45U2-71[»]
2Z4MX-ray4.45U2-71[»]
3DF1X-ray3.50U1-71[»]
3DF3X-ray3.50U1-71[»]
3FIHelectron microscopy-U4-54[»]
ModBaseSearch...

Genome annotation databases

GeneID947577.
GenomeReviewsGene locus JW3037 in contig AP009048_GR.
Gene locus b3065 in contig U00096_GR.
KEGGecj:JW3037.
eco:b3065.

Organism-specific databases

EchoBASEEB0913.
EcoGeneEG10920. rpsU.
CMRSearch...

Phylogenomic databases

HOGENOMP68679.
OMAP68679. PFERKPP.

Enzyme and pathway databases

BioCycEcoCyc:EG10920-MON.

Family and domain databases

HAMAPMF_00358.
[Tree]
InterProIPR001911. Ribosomal_S21.
IPR018278. Ribosomal_S21_CS.
[Graphical view]
PfamPF01165. Ribosomal_S21. 1 hit.
[Graphical view]
PRINTSPR00976. RIBOSOMALS21.
ProDomPD005521. Ribosomal_S21. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR00030. S21p. 1 hit.
PROSITEPS01181. RIBOSOMAL_S21. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRS21_ECOLI
AccessionPrimary (citable) accession number: P68679
Secondary accession number(s): P02379, Q2M9E0, Q8ZI69
Entry history
Integrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: June 16, 2009
This is version 49 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Ribosomal proteins

Ribosomal proteins families and list of entries

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents