Reviewed, UniProtKB/Swiss-Prot P68679 (RS21_ECOLI)

Names and origin

Protein names

Recommended name:
    30S ribosomal protein S21

Gene names

Name:	rpsU
Ordered Locus Names:	b3065, JW3037

Organism

Escherichia coli (strain K12) [Complete proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	71 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Sequence similarities	Belongs to the ribosomal protein S21P family.
Mass spectrometry	Molecular mass is 8368.8 Da from positions 2 - 71. Determined by MALDI. Ref.7

Ontologies

Keywords
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Cellular component	ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.5

Chain

2 – 71

70

30S ribosomal protein S21 HAMAP MF_00358

PRO_0000178332

Secondary structure

1

.

.

.

.

.

.

.

.

.

71

Helix Strand Turn

Details...

Helix

17 – 19

3

Turn

20 – 23

4

Helix

25 – 28

4

Turn

29 – 32

4

Helix

39 – 51

13

Sequences

	Sequence		Length	Mass (Da)
	P68679-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: B2E8A18BFBB2F685	FASTA	71	8,500
	10 20 30 40 50 60 MPVIKVRENE PFDVALRRFK RSCEKAGVLA EVRRREFYEK PTTERKRAKA SAVKRHAKKL 70 ARENARRTRL Y

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The operon that encodes the sigma subunit of RNA polymerase also encodes ribosomal protein S21 and DNA primase in E. coli K12." Burton Z.F., Gross C.A., Watanabe K.K., Burgess R.R. Cell 32:335-349(1983) [PubMed: 6186393] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[2]	"Regulation of the rpsU-dnaG-rpoD macromolecular synthesis operon and the initiation of DNA replication in Escherichia coli K-12." Lupski J.R., Smiley B.L., Godson G.N. Mol. Gen. Genet. 189:48-57(1983) [PubMed: 6222240] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: K12.
[3]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[4]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]	"Determination of the complete amino acid sequence of protein S21 from Escherichia coli ribosomes." Vandekerckhove J., Rombauts W., Peeters B., Wittmann-Liebold B. Hoppe-Seyler's Z. Physiol. Chem. 356:1955-1976(1975) [PubMed: 765257] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-71. Strain: K.
[6]	"Protein-rRNA binding features and their structural and functional implications in ribosomes as determined by cross-linking studies." Urlaub H., Kruft V., Bischof O., Mueller E.-C., Wittmann-Liebold B. EMBO J. 14:4578-4588(1995) [PubMed: 7556101] [Abstract] Cited for: PROTEIN SEQUENCE OF 26-35, CROSS-LINKING TO RRNA. Strain: MRE-600.
[7]	"Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry." Arnold R.J., Reilly J.P. Anal. Biochem. 269:105-112(1999) [PubMed: 10094780] [Abstract] Cited for: MASS SPECTROMETRY. Strain: K12 / ATCC 25404 / DSM 5698 / NCIMB 11290.
[8]	"Structures of the bacterial ribosome at 3.5 A resolution." Schuwirth B.S., Borovinskaya M.A., Hau C.W., Zhang W., Vila-Sanjurjo A., Holton J.M., Cate J.H.D. Science 310:827-834(2005) [PubMed: 16272117] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.46 ANGSTROMS) OF 2 DIFFERENT RIBOSOME STRUCTURES. Strain: MRE-600.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

J01687 Genomic DNA. Translation: AAA24599.1.
V00346 Genomic DNA. Translation: CAA23635.1.
U28379 Genomic DNA. Translation: AAA89145.1.
U00096 Genomic DNA. Translation: AAC76101.1.
AP009048 Genomic DNA. Translation: BAE77116.1.

PIR

R3EC21. A02749.

RefSeq

AP_003615.1.
NP_417537.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1VS5	X-ray	3.46	U	1-71	[»]
1VS7	X-ray	3.46	U	1-71	[»]
2AVY	X-ray	3.46	U	1-71	[»]
2AW7	X-ray	3.46	U	1-71	[»]
2I2P	X-ray	3.22	U	2-70	[»]
2I2U	X-ray	3.22	U	2-70	[»]
2QAL	X-ray	3.21	U	2-71	[»]
2QAN	X-ray	3.21	U	2-71	[»]
2QB9	X-ray	3.54	U	2-71	[»]
2QBB	X-ray	3.54	U	2-71	[»]
2QBD	X-ray	3.30	U	2-71	[»]
2QBF	X-ray	3.30	U	2-71	[»]
2QBH	X-ray	4.00	U	2-71	[»]
2QBJ	X-ray	4.00	U	2-71	[»]
2QOU	X-ray	3.93	U	2-71	[»]
2QOW	X-ray	3.93	U	2-71	[»]
2QOY	X-ray	3.50	U	2-71	[»]
2QP0	X-ray	3.50	U	2-71	[»]
2VHO	X-ray	3.74	U	1-71	[»]
2VHP	X-ray	3.74	U	1-71	[»]
2Z4K	X-ray	4.45	U	2-71	[»]
2Z4M	X-ray	4.45	U	2-71	[»]
3DF1	X-ray	3.50	U	1-71	[»]
3DF3	X-ray	3.50	U	1-71	[»]
3FIH	electron microscopy	-	U	4-54	[»]

ModBase

Search...

Genome annotation databases

GeneID

947577.

GenomeReviews

Gene locus JW3037 in contig AP009048_GR.
Gene locus b3065 in contig U00096_GR.

KEGG

ecj:JW3037.
eco:b3065.

Organism-specific databases

EchoBASE

EB0913.

EcoGene

EG10920. rpsU.

CMR

Search...

Phylogenomic databases

HOGENOM

P68679.

OMA

P68679. PFERKPP.

Enzyme and pathway databases

BioCyc

EcoCyc:EG10920-MON.

Family and domain databases

HAMAP

MF_00358.
[Tree]

InterPro

IPR001911. Ribosomal_S21.
IPR018278. Ribosomal_S21_CS.
[Graphical view]

Pfam

PF01165. Ribosomal_S21. 1 hit.
[Graphical view]

PRINTS

PR00976. RIBOSOMALS21.

ProDom

PD005521. Ribosomal_S21. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR00030. S21p. 1 hit.

PROSITE

PS01181. RIBOSOMAL_S21. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

RS21_ECOLI

Accession

Primary (citable) accession number: P68679
Secondary accession number(s): P02379, Q2M9E0, Q8ZI69

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 49 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)