P68646 (FIXX_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ferredoxin-like protein fixX

Gene names

Name:	fixX
Synonyms:	yaaT
Ordered Locus Names:	b0044, JW0043

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	95 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Could be part of an electron transfer system required for anaerobic carnitine reduction. Could be a 3Fe-4S cluster-containing protein. Ref.5
Sequence similarities	Belongs to the bacterial-type ferredoxin family. FixX subfamily.

Ontologies

Keywords
Biological process	Electron transport Transport
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 95	95	Ferredoxin-like protein fixX		PRO_0000159212

Sequences

	Sequence		Length	Mass (Da)
	P68646 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 999A9C8D5B694BE3	FASTA	95	10,479
	10 20 30 40 50 60 MTSPVNVDVK LGVNKFNVDE EHPHIVVKAD ADKQALELLV KACPAGLYKK QDDGSVRFDY 70 80 90 AGCLECGTCR ILGLGSALEQ WEYPRGTFGV EFRYG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed: 1630901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 35 AND 95. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The fix Escherichia coli region contains four genes related to carnitine metabolism." Eichler K., Buchet A., Bourgis F., Kleber H.-P., Mandrand-Berthelot M.-A. J. Basic Microbiol. 35:217-227(1995) [PubMed: 7473063] [Abstract] Cited for: GENE NAME. Strain: O44:K74.
[5]	"The fixA and fixB genes are necessary for anaerobic carnitine reduction in Escherichia coli." Walt A., Kahn M.L. J. Bacteriol. 184:4044-4047(2002) [PubMed: 12081978] [Abstract] Cited for: PROBABLE FUNCTION. Strain: K12 / BW25113.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC73155.1. AP009048 Genomic DNA. Translation: BAB96612.2.
PIR	D64725.
RefSeq	NP_414586.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P68646.
SMR	P68646. Positions 36-79.
ModBase	Search...
Protein-protein interaction databases
IntAct	P68646. 4 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000002924; EBESCP00000002924; EBESCG00000002389. EBESCT00000014537; EBESCP00000013828; EBESCG00000013598.
GeneID	948590.
GenomeReviews	Gene locus JW0043 in contig AP009048_GR. Gene locus b0044 in contig U00096_GR.
KEGG	ecj:JW0043. eco:b0044.
PATRIC	32115187. VBIEscCol129921_0045.
Organism-specific databases
EchoBASE	EB1526.
EcoGene	EG11565. fixX.
Phylogenomic databases
eggNOG	COG2440.
GeneTree	EBGT00050000009379.
HOGENOM	HBG498683.
OMA	PARCYEL.
PhylomeDB	P68646.
ProtClustDB	PRK15449.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11565-MONOMER.
Gene expression databases
Genevestigator	P68646.
Family and domain databases
InterPro	IPR012206. Fd_FixX. [Graphical view]
KO	K03855.
PIRSF	PIRSF036548. Fdx_FixX. 1 hit.
PROSITE	PS00198. 4FE4S_FER_1. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FIXX_ECOLI

Accession

Primary (citable) accession number: P68646
Secondary accession number(s): P31576, P75627

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	December 7, 2004
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program