P68644 (FIXC_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein fixC

Gene names

Name:	fixC
Synonyms:	yaaS
Ordered Locus Names:	b0043, JW0042

Organism

Escherichia coli (strain K12)

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia

Protein attributes

Sequence length	428 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Could be part of an electron transfer system required for anaerobic carnitine reduction. Ref.5
Cofactor	FAD Potential.
Sequence similarities	Belongs to the ETF-QO/fixC family.

Ontologies

Keywords
Biological process	Electron transport Transport
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	carnitine metabolic process Inferred from expression pattern Ref.4. Source: EcoliWiki electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	oxidoreductase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 428	428	Protein fixC		PRO_0000200690
Regions
	Nucleotide binding	12 – 28	17	FAD or NAD or NADP Potential
Experimental info
	Sequence conflict	1 – 3	3	MSE → MT Ref.4
	Sequence conflict	64	1	D → E in CAA50799. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P68644 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: 4D089EE9A30C7FBC	FASTA	428	45,703
	10 20 30 40 50 60 MSEDIFDAII VGAGLAGSVA ALVLAREGAQ VLVIERGNSA GAKNVTGGRL YAHSLEHIIP 70 80 90 100 110 120 GFADSAPVER LITHEKLAFM TEKSAMTMDY CNGDETSPSQ RSYSVLRSKF DAWLMEQAEE 130 140 150 160 170 180 AGAQLITGIR VDNLVQRDGK VVGVEADGDV IEAKTVILAD GVNSILAEKL GMAKRVKPTD 190 200 210 220 230 240 VAVGVKELIE LPKSVIEDRF QLQGNQGAAC LFAGSPTDGL MGGGFLYTNE NTLSLGLVCG 250 260 270 280 290 300 LHHLHDAKKS VPQMLEDFKQ HPAVAPLIAG GKLVEYSAHV VPEAGINMLP ELVGDGVLIA 310 320 330 340 350 360 GDAAGMCMNL GFTIRGMDLA IAAGEAAAKT VLSAMKSDDF SKQKLAEYRQ HLESGPLRDM 370 380 390 400 410 420 RMYQKLPAFL DNPRMFSGYP ELAVGVARDL FTIDGSAPEL MRKKILRHGK KVGFINLIKD GMKGVTVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Nucleic Acids Res. 20:3305-3308(1992) [PubMed: 1630901] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed: 9278503] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed: 16738553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION TO 64; 280 AND 310. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"The fix Escherichia coli region contains four genes related to carnitine metabolism." Eichler K., Buchet A., Bourgis F., Kleber H.-P., Mandrand-Berthelot M.-A. J. Basic Microbiol. 35:217-227(1995) [PubMed: 7473063] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-188. Strain: O44:K74.
[5]	"The fixA and fixB genes are necessary for anaerobic carnitine reduction in Escherichia coli." Walt A., Kahn M.L. J. Bacteriol. 184:4044-4047(2002) [PubMed: 12081978] [Abstract] Cited for: PROBABLE FUNCTION. Strain: K12 / BW25113.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC73154.1. AP009048 Genomic DNA. Translation: BAB96611.2. X71977 Genomic DNA. Translation: CAA50799.1.
PIR	C64725.
RefSeq	NP_414585.1. NC_000913.2.
3D structure databases
ProteinModelPortal	P68644.
SMR	P68644. Positions 5-404.
ModBase	Search...
Protein-protein interaction databases
IntAct	P68644. 7 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBESCT00000000203; EBESCP00000000203; EBESCG00000000162. EBESCT00000015856; EBESCP00000015147; EBESCG00000014916.
GeneID	948958.
GenomeReviews	Gene locus JW0042 in contig AP009048_GR. Gene locus b0043 in contig U00096_GR.
KEGG	ecj:JW0042. eco:b0043.
PATRIC	32115185. VBIEscCol129921_0044.
Organism-specific databases
EchoBASE	EB1525.
EcoGene	EG11564. fixC.
Phylogenomic databases
eggNOG	COG0644.
GeneTree	EBGT00050000009863.
HOGENOM	HBG295204.
OMA	YPEMAVN.
PhylomeDB	P68644.
ProtClustDB	PRK10157.
Enzyme and pathway databases
BioCyc	EcoCyc:EG11564-MONOMER.
Gene expression databases
Genevestigator	P68644.
Family and domain databases
InterPro	IPR006076. FAD-dep_OxRdtase. IPR003042. Rng_hydrolase-like. [Graphical view]
KO	K00313.
Pfam	PF01266. DAO. 2 hits. [Graphical view]
PRINTS	PR00420. RNGMNOXGNASE.
ProtoNet	Search...

Entry information

Entry name

FIXC_ECOLI

Accession

Primary (citable) accession number: P68644
Secondary accession number(s): P31575, P75626

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	December 7, 2004
Last modified:	January 25, 2012
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program