Reviewed, UniProtKB/Swiss-Prot P68641 (ASCD_YERPE)

Names and origin

Protein names

Recommended name:
    CDP-6-deoxy-L-threo-D-glycero-4-hexulose-3-dehydrase reductase
    EC=1.17.1.-
Alternative name(s):
    CDP-6-deoxy-delta-3,4-glucoseen reductase
      Short name=E3

Gene names

Name:	ascD
Synonyms:	ddhD, rfbI
Ordered Locus Names:	YPO3116, y1067, YP_0814

Organism

Yersinia pestis [Complete proteome] [HAMAP]

Taxonomic identifier

632 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Yersinia

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Participates in the conversion of CDP-6-deoxy-D-glycero-L-threo-4-hexulose to 3,6-dideoxy-D-glycero-D-glycero-4-hexulose together with CDP-6-deoxy-D-glycero-L-threo-4-hexulose-3-dehydrase (E1) in two consecutive steps. The detailed mechanism of E3 is not yet resolved By similarity.
Pathway	Nucleotide-sugar biosynthesis; CDP-ascarylose biosynthesis. Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis.
Subunit structure	Monomer By similarity.
Sequence similarities	Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 FAD-binding FR-type domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 329	328	CDP-6-deoxy-L-threo-D-glycero-4-hexulose-3-dehydrase reductase		PRO_0000189393
Regions
	Domain	2 – 93	92	2Fe-2S ferredoxin-type
	Domain	98 – 197	100	FAD-binding FR-type
Sites
	Metal binding	37	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	42	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	45	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	75	1	Iron-sulfur (2Fe-2S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P68641-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 7930BD82FF126503	FASTA	329	36,047
	10 20 30 40 50 60 MSLNVKLHPS GIIFTSDGTS TILDAALDSN IHIEYSCKDG TCGSCKAILI SGEVDSAENT 70 80 90 100 110 120 FLTEEDVAKG AILTCCSKAK SDIELDVNYY PELSHIQKKT YPCKLDSIEF IGEDIAILSL 130 140 150 160 170 180 RLPPTAKIQY LAGQYIDLII NGQRRSYSIA NAPGGNGNIE LHVRKVVNGV FSNIIFNELK 190 200 210 220 230 240 LQQLLRIEGP QGTFFVREDN LPIVFLAGGT GFAPVKSMVE ALINKNDQRQ VHIYWGMPAG 250 260 270 280 290 300 HNFYSDIANE WAIKHPNIHY VPVVSGDDST WTGATGFVHQ AVLEDIPDLS LFNVYACGSL 310 320 AMITAARNDF INHGLAENKF FSDAFVPSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the O-antigen gene clusters of Yersinia pseudotuberculosis and the cryptic O-antigen gene cluster of Yersinia pestis shows that the plague bacillus is most closely related to and has evolved from Y. pseudotuberculosis serotype O:1b." Skurnik M., Peippo A., Ervela E. Mol. Microbiol. 37:316-330(2000) [PubMed: 10931327] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: EV 76.
[2]	"Genome sequence of Yersinia pestis, the causative agent of plague." Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G., Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L., Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M., Chillingworth T., Cronin A., Davies R.M. , Davis P., Dougan G., Feltwell T., Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S., Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S., Barrell B.G. Nature 413:523-527(2001) [PubMed: 11586360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CO-92 / Biovar Orientalis.
[3]	"Genome sequence of Yersinia pestis KIM." Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P., Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D., Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A., Nilles M.L. , Matson J.S., Blattner F.R., Perry R.D. J. Bacteriol. 184:4601-4611(2002) [PubMed: 12142430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KIM5 / Biovar Mediaevalis.
[4]	"Complete genome sequence of Yersinia pestis strain 91001, an isolate avirulent to humans." Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D., Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L., Dai R. , Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H., Wang J., Huang P., Yang R. DNA Res. 11:179-197(2004) [PubMed: 15368893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 91001 / Biovar Mediaevalis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ251713 Genomic DNA. Translation: CAB63270.1. AL590842 Genomic DNA. Translation: CAL21712.1. AE009952 Genomic DNA. Translation: AAM84648.1. AE017042 Genomic DNA. Translation: AAS61079.1.
PIR	AE0378.
RefSeq	NP_668397.1. NP_992202.1.
3D structure databases
HSSP	HSSP built from PDB template 1QFJ based on UniProtKB P23486.
ModBase	Search...
Genome annotation databases
GeneID	1146014. 2764213.
GenomeReviews	Gene locus y1067 in contig AE009952_GR. Gene locus YP_0814 in contig AE017042_GR. Gene locus YPO3116 in contig AL590842_GR.
KEGG	ype:YPO3116. ypk:y1067. ypm:YP_0814.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	P68641.
Enzyme and pathway databases
BioCyc	YPES187410:Y1067-MON. YPES214092:YPO3116-MON. YPES229193:YP0814-MON.
Family and domain databases
InterPro	IPR006058. 2Fe2S_fd_BS. IPR012675. b-grasp_ferredoxin-like. IPR001041. Ferredoxin. IPR008333. OxRdtase_FAD-bd. IPR001433. OxRdtase_FAD/NAD_bd. IPR001221. Phe_hydroxylase. [Graphical view]
Gene3D	G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit.
Pfam	PF00970. FAD_binding_6. 1 hit. PF00111. Fer2. 1 hit. PF00175. NAD_binding_1. 1 hit. [Graphical view]
PRINTS	PR00410. PHEHYDRXLASE.
PROSITE	PS00197. 2FE2S_FER_1. 1 hit. PS51085. 2FE2S_FER_2. 1 hit. PS51384. FAD_FR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASCD_YERPE

Accession

Primary (citable) accession number: P68641
Secondary accession number(s): P37911, Q7B0V7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 37 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)