Reviewed, UniProtKB/Swiss-Prot P68638 (VCP_VACCW)

Names and origin

Protein names

Recommended name:
    Complement control protein
Alternative name(s):
    VCP
    Secretory protein 35
      Short name=Protein C3
    28 kDa protein

Gene names

ORF Names:

C3L

Organism

Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))

Taxonomic identifier

10254 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Orthopoxvirus › Vaccinia virus

Virus host

Bos taurus (Bovine) [TaxID: 9913]

Protein attributes

Sequence length	263 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Serves to protect the virus against complement attack by inhibiting both classical and alternative pathways of complement activation. Binds C3b and C4b. Ref.3
Sequence similarities	Belongs to the receptors of complement activation (RCA) family. Contains 4 Sushi (CCP/SCR) domains.

Ontologies

Keywords
Domain	Repeat Signal Sushi
PTM	Disulfide bond
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	negative regulation of complement activation Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: InterPro
Molecular function	complement binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

19

Ref.1

Chain

20 – 263

244

Complement control protein

PRO_0000006024

Regions

Domain

20 – 83

64

Sushi 1

Domain

84 – 145

62

Sushi 2

Domain

146 – 203

58

Sushi 3

Domain

204 – 263

60

Sushi 4

Amino acid modifications

Disulfide bond

21 ↔ 70

Ref.5

Disulfide bond

54 ↔ 81

Ref.5

Disulfide bond

86 ↔ 126

Ref.5

Disulfide bond

112 ↔ 143

Ref.5

Disulfide bond

148 ↔ 190

Ref.5

Disulfide bond

176 ↔ 201

Ref.5

Disulfide bond

206 ↔ 248

Ref.5

Disulfide bond

234 ↔ 261

Ref.5

Secondary structure

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263

Helix Strand Turn

Details...

Beta strand

44 – 46

3

Beta strand

72 – 74

3

Beta strand

96 – 99

4

Beta strand

122 – 126

5

Beta strand

129 – 132

4

Beta strand

136 – 138

3

Beta strand

143 – 146

4

Beta strand

157 – 159

3

Beta strand

162 – 165

4

Beta strand

168 – 172

5

Beta strand

174 – 176

3

Beta strand

181 – 193

13

Beta strand

200 – 203

4

Beta strand

212 – 216

5

Beta strand

232 – 234

3

Beta strand

239 – 242

4

Turn

250 – 252

3

Beta strand

253 – 256

4

Beta strand

260 – 262

3

Sequences

	Sequence		Length	Mass (Da)
	P68638-1 [UniParc]. Last modified December 7, 2004. Version 1. Checksum: E4322CC9A6EF8997	FASTA	263	28,629
	10 20 30 40 50 60 MKVESVTFLT LLGIGCVLSC CTIPSRPINM KFKNSVETDA NANYNIGDTI EYLCLPGYRK 70 80 90 100 110 120 QKMGPIYAKC TGTGWTLFNQ CIKRRCPSPR DIDNGQLDIG GVDFGSSITY SCNSGYHLIG 130 140 150 160 170 180 ESKSYCELGS TGSMVWNPEA PICESVKCQS PPSISNGRHN GYEDFYTDGS VVTYSCNSGY 190 200 210 220 230 240 SLIGNSGVLC SGGEWSDPPT CQIVKCPHPT ISNGYLSSGF KRSYSYNDNV DFKCKYGYKL 250 260 SGSSSSTCSP GNTWKPELPK CVR

References

[1]	"Vaccinia virus encodes a secretory polypeptide structurally related to complement control proteins." Kotwal G.J., Moss B. Nature 335:176-178(1988) [PubMed: 3412473] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 20-37.
[2]	"Analysis of a large cluster of nonessential genes deleted from a vaccinia virus terminal transposition mutant." Kotwal G.J., Moss B. Virology 167:524-537(1988) [PubMed: 2849238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Vaccinia virus complement-control protein prevents antibody-dependent complement-enhanced neutralization of infectivity and contributes to virulence." Isaacs S.N., Kotwal G.J., Moss B. Proc. Natl. Acad. Sci. U.S.A. 89:628-632(1992) [PubMed: 1731333] [Abstract] Cited for: FUNCTION.
[4]	"NMR studies of a viral protein that mimics the regulators of complement activation." Wiles A.P., Shaw G., Bright J., Perczel A., Campbell I.D., Barlow P.N. J. Mol. Biol. 272:253-265(1997) [PubMed: 9299352] [Abstract] Cited for: STRUCTURE BY NMR OF 146-263.
[5]	"Crystal structure of a complement control protein that regulates both pathways of complement activation and binds heparan sulfate proteoglycans." Murthy K.H.M., Smith S.A., Ganesh V.K., Judge K.W., Mullin N., Barlow P.N., Ogata C.M., Kotwal G.J. Cell 104:301-311(2001) [PubMed: 11207370] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 20-263, DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X13166 Genomic DNA. Translation: CAA31564.1.
M22812 Genomic DNA. Translation: AAA69605.1.

PIR

WMVZSP. A31005.

RefSeq

YP_232907.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1E5G	NMR	-	A	84-203	[»]
1G40	X-ray	2.20	A/B	20-263	[»]
1G44	X-ray	2.60	A/B/C	20-263	[»]
1RID	X-ray	2.10	A/B	20-263	[»]
1VVC	NMR	-	A	146-263	[»]
1VVD	NMR	-	A	146-263	[»]
1VVE	NMR	-	A	146-263	[»]
1Y8E	X-ray	2.20	A/B	20-263	[»]

ModBase

Search...

Genome annotation databases

GeneID

3707640.

Family and domain databases

InterPro

IPR011176. CIP_VAC_C3L.
IPR016060. Complement_control_module.
IPR000436. Sushi_SCR_CCP.
[Graphical view]

Gene3D

G3DSA:2.10.70.10. Complement_control_module. 4 hits.

Pfam

PF00084. Sushi. 4 hits.
[Graphical view]

PIRSF

PIRSF002486. CIP_VAC_C3L. 1 hit.

SMART

SM00032. CCP. 4 hits.
[Graphical view]

PROSITE

PS50923. SUSHI. 4 hits.
[Graphical view]

ProtoNet

Search...

Other Resources

DrugBank

DB04786. Suramin.

Entry information

Entry name

VCP_VACCW

Accession

Primary (citable) accession number: P68638
Secondary accession number(s): P10998

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	December 7, 2004
Last modified:	December 15, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)