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Protein

Protein A36

Gene

VACWR159

Organism
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the intracellular transport of virions to the host cell surface. Participates also in the formation of actin tails at the plasma membrane to allow efficient actin-based motility and thus cell to cell transmission of viral particles. Recruits host intersectin-1/ITSN1 and activates host CDC42 to drive ARP2/3-mediated actin polymerization.4 Publications

GO - Biological processi

  • actin-dependent intracellular transport of virus Source: UniProtKB
  • viral release from host cell Source: UniProtKB

Keywordsi

Biological processHost-virus interaction

Names & Taxonomyi

Protein namesi
Recommended name:
Protein A36
Gene namesi
Ordered Locus Names:VACWR159
ORF Names:A36R
OrganismiVaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR))
Taxonomic identifieri10254 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePoxviridaeChordopoxvirinaeOrthopoxvirusVaccinia virus
Virus hostiBos taurus (Bovine) [TaxID: 9913]
Proteomesi
  • UP000000344 Componenti: Genome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1Extracellular1
Transmembranei2 – 22HelicalSequence analysisAdd BLAST21
Topological domaini23 – 221CytoplasmicAdd BLAST199

GO - Cellular componenti

  • host cell plasma membrane Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW

Keywords - Cellular componenti

Host cell membrane, Host membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi112Y → F: About 85% loss of virus-induced actin tail formation. 1 Publication1
Mutagenesisi112Y → F: Complete loss of virus-induced actin tail formation; when associated with Y-132. 1 Publication1
Mutagenesisi132Y → F: About 20% loss of virus-induced actin tail formation. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000406001 – 221Protein A36Add BLAST221

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei112Phosphotyrosine; by host1 Publication1
Modified residuei132Phosphotyrosine; by host1 Publication1

Post-translational modificationi

Phosphorylation of Tyr-112 and Tyr-132 of A36 Mechanistically, phosphorylation of Tyr-112 and Tyr-132 of A36 activates the host ARP2-ARP3 complex and leads to actin nucleation.1 Publication

Keywords - PTMi

Phosphoprotein

PTM databases

iPTMnetiP68619.

Interactioni

Subunit structurei

Interacts with host NCK (PubMed:10553910). Interacts with protein A33 (via C-terminus) (PubMed:12634370). Interacts with protein F12 (PubMed:20195521). Interacts (via C-terminus) with host kinesin light chain/KLC1 (PubMed:14963148). Interacts with host intersectin-1/ITSN1 and EPS15 (PubMed:27670116).5 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

IntActiP68619. 7 interactors.
MINTiMINT-6740391.

Structurei

3D structure databases

ProteinModelPortaliP68619.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi161 – 163NPF-motif1 Publication3
Motifi176 – 178NPF-motif1 Publication3
Motifi190 – 192NPF-motif1 Publication3

Sequence similaritiesi

Belongs to the orthopoxvirus A36 protein family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

OrthoDBiVOG090000TQ.

Family and domain databases

InterProiView protein in InterPro
IPR010274. Orthopox_A36R.
PfamiView protein in Pfam
PF05950. Orthopox_A36R. 1 hit.
ProDomiView protein in ProDom or Entries sharing at least one domain
PD499178. Orthopox_A36R. 1 hit.

Sequencei

Sequence statusi: Complete.

P68619-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMLVPLITVT VVAGTILVCY ILYICRKKIR TVYNDNKIIM TKLKKIKSSN
60 70 80 90 100
SSKSSKSTDS ESDWEDHCSA MEQNNDVDNI SRNEILDDDS FAGSLIWDNE
110 120 130 140 150
SNVMAPSTEH IYDSVAGSTL LINNDRNEQT IYQNTTVVIN ETETVEVLNE
160 170 180 190 200
DTKQNPNYSS NPFVNYNKTS ICSKSNPFIT ELNNKFSENN PFRRAHSDDY
210 220
LNKQEQDHEH DDIESSVVSL V
Length:221
Mass (Da):25,133
Last modified:December 7, 2004 - v1
Checksum:i5D15758981B0E2E2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D11079 Genomic DNA. Translation: BAA01808.1.
M61187 Genomic DNA. Translation: AAA48332.1.
X57318 Genomic DNA. Translation: CAA40585.1.
AY243312 Genomic DNA. Translation: AAO89438.1.
PIRiJQ1772. B42521.
RefSeqiYP_233041.1. NC_006998.1.

Genome annotation databases

GeneIDi3707689.
KEGGivg:3707689.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiA36_VACCW
AccessioniPrimary (citable) accession number: P68619
Secondary accession number(s): P21059, Q76ZP0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 7, 2004
Last sequence update: December 7, 2004
Last modified: June 7, 2017
This is version 48 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families